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Protein

Basic phospholipase A2 RV-4

Gene
N/A
Organism
Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Heterodimer RV-4/RV-7: acts as a presynaptic neurotoxin.
Monomer: snake venom phospholipase A2 (PLA2) that acts as a presynaptic neurotoxin. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43Calcium; via carbonyl oxygenBy similarity1
Metal bindingi45Calcium; via carbonyl oxygenBy similarity1
Metal bindingi47Calcium; via carbonyl oxygenBy similarity1
Active sitei63By similarity1
Metal bindingi64CalciumBy similarity1
Active sitei105By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Neurotoxin, Presynaptic neurotoxin, Toxin
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Basic phospholipase A2 RV-4 (EC:3.1.1.4)
Short name:
svPLA2
Alternative name(s):
F4
Phosphatidylcholine 2-acylhydrolase
S2
Viperotoxin F
Viperotoxin toxic basic component
OrganismiDaboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Taxonomic identifieri343250 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeDaboia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.32 mg/kg by intravenous injection for RV-4 and 0.15 mg/kg for RV-4/RV-7 complex.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 162 PublicationsAdd BLAST16
ChainiPRO_000002297717 – 138Basic phospholipase A2 RV-4Add BLAST122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 1311 Publication
Disulfide bondi44 ↔ 601 Publication
Disulfide bondi59 ↔ 1111 Publication
Disulfide bondi65 ↔ 1381 Publication
Disulfide bondi66 ↔ 1041 Publication
Disulfide bondi73 ↔ 971 Publication
Disulfide bondi91 ↔ 1021 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ02471

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Heterodimer of a weakly toxic basic protein having phospholipase A2 activity (RV-4) and a non-toxic acidic protein which inhibits its enzymatic activity but potentiates its lethal potency and neurotoxicity (RV-7).

Structurei

Secondary structure

1138
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 29Combined sources12
Helixi33 – 37Combined sources5
Beta strandi38 – 40Combined sources3
Turni41 – 43Combined sources3
Beta strandi44 – 46Combined sources3
Helixi55 – 68Combined sources14
Turni75 – 77Combined sources3
Beta strandi82 – 85Combined sources4
Beta strandi88 – 91Combined sources4
Helixi97 – 114Combined sources18
Turni115 – 118Combined sources4
Helixi121 – 123Combined sources3
Helixi128 – 131Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OQSX-ray1.90B/D/F/H17-138[»]
ProteinModelPortaliQ02471
SMRiQ02471
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02471

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLWIVAVC LIGVEGNLFQ FARMINGKLG AFSVWNYISY GCYCGWGGQG
60 70 80 90 100
TPKDATDRCC FVHDCCYGGV KGCNPKLAIY SYSFQRGNIV CGRNNGCLRT
110 120 130
ICECDRVAAN CFHQNKNTYN KEYKFLSSSK CRQRSEQC
Length:138
Mass (Da):15,555
Last modified:July 1, 1993 - v1
Checksum:iBFD471C8BBFE7F77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68385 mRNA Translation: CAA48456.1

Similar proteinsi

Entry informationi

Entry nameiPA2B4_DABSI
AccessioniPrimary (citable) accession number: Q02471
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

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