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Q02455

- MLP1_YEAST

UniProt

Q02455 - MLP1_YEAST

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Protein
Protein MLP1
Gene
MLP1, YKR095W, YKR415
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved together with the closely related MLP2 in the structural and functional organization of perinuclear chromatin. MLP1/MLP2 associate with the nuclear pore complex and form filamentous structures along the nuclear periphery. According to 1 Publication some telomeres are tethered to the nuclear periphery through MLP1/MLP2, probably mediated by YKU70/YKU80 (HDF1/HDF2) heterodimer and show perinuclear location dependent silencing. According to 1 Publication MLP1 and MLP2 are involved in telomere length regulation but not silencing or telomere anchoring. MLP1 also recognizes the 5'-splice site of pre-mRNAs and retains unspliced pre-mRNA in the nucleus without affecting splicing itself.6 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. ribonucleoprotein complex binding Source: SGD

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. negative regulation of protein import into nucleus during spindle assembly checkpoint Source: SGD
  3. nuclear retention of unspliced pre-mRNA at the site of transcription Source: SGD
  4. poly(A)+ mRNA export from nucleus Source: SGD
  5. protein import into nucleus Source: SGD
  6. protein localization to nuclear pore Source: SGD
  7. telomere tethering at nuclear periphery Source: SGD
  8. transcriptional activation by promoter-terminator looping Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, mRNA transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32058-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MLP1
Alternative name(s):
Myosin-like protein 1
Gene namesi
Name:MLP1
Ordered Locus Names:YKR095W
ORF Names:YKR415
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR095w.
SGDiS000001803. MLP1.

Subcellular locationi

Nucleus
Note: Distributed fairly evenly along a C-shaped portion of the nuclear periphery, where the spindle pole body localizes in 90% of the cases.2 Publications

GO - Cellular componenti

  1. nuclear envelope Source: SGD
  2. nuclear pore nuclear basket Source: SGD
  3. nucleoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 18751874Protein MLP1
PRO_0000096501Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei337 – 3371Phosphothreonine2 Publications
Modified residuei379 – 3791Phosphoserine1 Publication
Modified residuei1670 – 16701Phosphoserine1 Publication
Modified residuei1710 – 17101Phosphoserine2 Publications
Modified residuei1733 – 17331Phosphoserine1 Publication
Modified residuei1803 – 18031Phosphoserine1 Publication

Post-translational modificationi

May be phosphorylated by CDC28.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ02455.
PaxDbiQ02455.
PeptideAtlasiQ02455.
PRIDEiQ02455.

Expressioni

Gene expression databases

GenevestigatoriQ02455.

Interactioni

Subunit structurei

Interacts with NAB2, a hnRNP required for mRNA export. Also interacts with MLP2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MLP2P404572EBI-11009,EBI-25261
NAB2P325054EBI-11009,EBI-11770

Protein-protein interaction databases

BioGridi34226. 101 interactions.
DIPiDIP-6675N.
IntActiQ02455. 8 interactions.
MINTiMINT-622372.
STRINGi4932.YKR095W.

Structurei

3D structure databases

ProteinModelPortaliQ02455.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili69 – 487419 Reviewed prediction
Add
BLAST
Coiled coili531 – 16781148 Reviewed prediction
Add
BLAST
Coiled coili1834 – 186633 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1496 – 156570Required for nuclear localization
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1804 – 186562Glu-rich
Add
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00730000111014.
HOGENOMiHOG000113604.
KOiK09291.
OMAiTRESTEN.
OrthoDBiEOG7BP89V.

Family and domain databases

InterProiIPR001611. Leu-rich_rpt.
IPR012929. TPR_MLP1_2.
[Graphical view]
PfamiPF07926. TPR_MLP1_2. 1 hit.
[Graphical view]
PROSITEiPS51450. LRR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02455-1 [UniParc]FASTAAdd to Basket

« Hide

MSDHDTPMES IQNGENSDER LNAIASFFGC SLEQVKSFDG DVVKHLNDKL     50
LQFNELKSEN LKVTVSFDEL KASSLKKIDG LKTEMENVIR ENDKIRKERN 100
DTFVKFESVE NEKMKLSSEL EFVKRKLDDL TEEKKETQSN QQRTLKILDE 150
RLKEIELVRV ENNRSNSECK KLRSTIMDLE TKQQGYITND LNSRTELERK 200
TQELTLLQSN NDWLEKELRS KNEQYLSYRQ KTDKVILDIR NELNRLRNDF 250
QMERTNNDVL KQKNNELSKS LQEKLLEIKG LSDSLNSEKQ EFSAEMSLKQ 300
RLVDLLESQL NAVKEELNSI RELNTAKVIA DDSKKQTPEN EDLLKELQLT 350
KEKLAQCEKE CLRLSSITDE ADEDNENLSA KSSSDFIFLK KQLIKERRTK 400
EHLQNQIETF IVELEHKVPI INSFKERTDM LENELNNAAL LLEHTSNEKN 450
AKVKELNAKN QKLVECENDL QTLTKQRLDL CRQIQYLLIT NSVSNDSKGP 500
LRKEEIQFIQ NIMQEDDSTI TESDSQKVVT ERLVEFKNII QLQEKNAELL 550
KVVRNLADKL ESKEKKSKQS LQKIESETVN EAKEAIITLK SEKMDLESRI 600
EELQKELEEL KTSVPNEDAS YSNVTIKQLT ETKRDLESQV QDLQTRISQI 650
TRESTENMSL LNKEIQDLYD SKSDISIKLG KEKSSRILAE ERFKLLSNTL 700
DLTKAENDQL RKRFDYLQNT ILKQDSKTHE TLNEYVSCKS KLSIVETELL 750
NLKEEQKLRV HLEKNLKQEL NKLSPEKDSL RIMVTQLQTL QKEREDLLEE 800
TRKSCQKKID ELEDALSELK KETSQKDHHI KQLEEDNNSN IEWYQNKIEA 850
LKKDYESVIT SVDSKQTDIE KLQYKVKSLE KEIEEDKIRL HTYNVMDETI 900
NDDSLRKELE KSKINLTDAY SQIKEYKDLY ETTSQSLQQT NSKLDESFKD 950
FTNQIKNLTD EKTSLEDKIS LLKEQMFNLN NELDLQKKGM EKEKADFKKR 1000
ISILQNNNKE VEAVKSEYES KLSKIQNDLD QQTIYANTAQ NNYEQELQKH 1050
ADVSKTISEL REQLHTYKGQ VKTLNLSRDQ LENALKENEK SWSSQKESLL 1100
EQLDLSNSRI EDLSSQNKLL YDQIQIYTAA DKEVNNSTNG PGLNNILITL 1150
RRERDILDTK VTVAERDAKM LRQKISLMDV ELQDARTKLD NSRVEKENHS 1200
SIIQQHDDIM EKLNQLNLLR ESNITLRNEL ENNNNKKKEL QSELDKLKQN 1250
VAPIESELTA LKYSMQEKEQ ELKLAKEEVH RWKKRSQDIL EKHEQLSSSD 1300
YEKLESEIEN LKEELENKER QGAEAEEKFN RLRRQAQERL KTSKLSQDSL 1350
TEQVNSLRDA KNVLENSLSE ANARIEELQN AKVAQGNNQL EAIRKLQEDA 1400
EKASRELQAK LEESTTSYES TINGLNEEIT TLKEEIEKQR QIQQQLQATS 1450
ANEQNDLSNI VESMKKSFEE DKIKFIKEKT QEVNEKILEA QERLNQPSNI 1500
NMEEIKKKWE SEHEQEVSQK IREAEEALKK RIRLPTEEKI NKIIERKKEE 1550
LEKEFEEKVE ERIKSMEQSG EIDVVLRKQL EAKVQEKQKE LENEYNKKLQ 1600
EELKDVPHSS HISDDERDKL RAEIESRLRE EFNNELQAIK KKSFDEGKQQ 1650
AMMKTTLLER KLAKMESQLS ETKQSAESPP KSVNNVQNPL LGLPRKIEEN 1700
SNSPFNPLLS GEKLLKLNSK SSSGGFNPFT SPSPNKHLQN DNDKRESLAN 1750
KTDPPTHLEP SFNIPASRGL ISSSSTLSTD TNDEELTSNN PAQKDSSNRN 1800
VQSEEDTEKK KEGEPVKRGE AIEEQTKSNK RPIDEVGELK NDEDDTTENI 1850
NESKKIKTED EEEKETDKVN DENSI 1875
Length:1,875
Mass (Da):218,456
Last modified:June 1, 1994 - v2
Checksum:i683A0D34C9066867
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti301 – 3011R → A in AAA34783. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01992 Genomic DNA. Translation: AAA34783.1.
X73541 Genomic DNA. Translation: CAA51948.1.
Z28320 Genomic DNA. Translation: CAA82174.1.
BK006944 Genomic DNA. Translation: DAA09245.1.
PIRiS38173.
RefSeqiNP_013021.1. NM_001179885.1.

Genome annotation databases

EnsemblFungiiYKR095W; YKR095W; YKR095W.
GeneIDi853970.
KEGGisce:YKR095W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01992 Genomic DNA. Translation: AAA34783.1 .
X73541 Genomic DNA. Translation: CAA51948.1 .
Z28320 Genomic DNA. Translation: CAA82174.1 .
BK006944 Genomic DNA. Translation: DAA09245.1 .
PIRi S38173.
RefSeqi NP_013021.1. NM_001179885.1.

3D structure databases

ProteinModelPortali Q02455.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34226. 101 interactions.
DIPi DIP-6675N.
IntActi Q02455. 8 interactions.
MINTi MINT-622372.
STRINGi 4932.YKR095W.

Protein family/group databases

TCDBi 1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

MaxQBi Q02455.
PaxDbi Q02455.
PeptideAtlasi Q02455.
PRIDEi Q02455.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKR095W ; YKR095W ; YKR095W .
GeneIDi 853970.
KEGGi sce:YKR095W.

Organism-specific databases

CYGDi YKR095w.
SGDi S000001803. MLP1.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00730000111014.
HOGENOMi HOG000113604.
KOi K09291.
OMAi TRESTEN.
OrthoDBi EOG7BP89V.

Enzyme and pathway databases

BioCyci YEAST:G3O-32058-MONOMER.

Miscellaneous databases

NextBioi 975413.
PROi Q02455.

Gene expression databases

Genevestigatori Q02455.

Family and domain databases

InterProi IPR001611. Leu-rich_rpt.
IPR012929. TPR_MLP1_2.
[Graphical view ]
Pfami PF07926. TPR_MLP1_2. 1 hit.
[Graphical view ]
PROSITEi PS51450. LRR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new yeast gene with a myosin-like heptad repeat structure."
    Koelling R., Nguyen T., Chen E.Y., Botstein D.
    Mol. Gen. Genet. 237:359-369(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The complete sequence of a 15,820 bp segment of Saccharomyces cerevisiae chromosome XI contains the UBI2 and MPL1 genes and three new open reading frames."
    Bou G., Esteban P.F., Baladron V., Gonzalez G.A., Cantalejo J.G., Remacha M.A., Jimenez A., del Rey F., Ballesta J.P.G., Revuelta J.L.
    Yeast 9:1349-1354(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Proteins connecting the nuclear pore complex with the nuclear interior."
    Strambio-de-Castillia C., Blobel G., Rout M.P.
    J. Cell Biol. 144:839-855(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, FORMATION OF CHROMATIN EXCLUDING FILAMENTOUS STRUCTURES WITH MLP2.
  6. "Nuclear pore complexes in the organization of silent telomeric chromatin."
    Galy V., Olivo-Marin J.-C., Scherthan H., Doye V., Rascalou N., Nehrbass U.
    Nature 403:108-112(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PERINUCLEAR TELOMERE CLUSTERING.
  7. "Nuclear architecture and spatial positioning help establish transcriptional states of telomeres in yeast."
    Feuerbach F., Galy V., Trelles-Sticken E., Fromont-Racine M., Jacquier A., Gilson E., Olivo-Marin J.-C., Scherthan H., Nehrbass U.
    Nat. Cell Biol. 4:214-221(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PERINUCLEAR-DEPENDENT SILENCING.
  8. "Myosin-like proteins 1 and 2 are not required for silencing or telomere anchoring, but act in the Tel1 pathway of telomere length control."
    Hediger F., Dubrana K., Gasser S.M.
    J. Struct. Biol. 140:79-91(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TELOMERE LENGTH REGULATION.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "The C-terminal domain of myosin-like protein 1 (Mlp1p) is a docking site for heterogeneous nuclear ribonucleoproteins that are required for mRNA export."
    Green D.M., Johnson C.P., Hagan H., Corbett A.H.
    Proc. Natl. Acad. Sci. U.S.A. 100:1010-1015(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NAB2.
  11. "Nuclear retention of unspliced mRNAs in yeast is mediated by perinuclear Mlp1."
    Galy V., Gadal O., Fromont-Racine M., Romano A., Jacquier A., Nehrbass U.
    Cell 116:63-73(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, NUCLEAR RETENTION OF UNSPLICED PRE-MRNA.
  12. Cited for: PHOSPHORYLATION BY CDC28.
  13. "The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly."
    Niepel M., Strambio-de-Castillia C., Fasolo J., Chait B.T., Rout M.P.
    J. Cell Biol. 170:225-235(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MLP2, SUBCELLULAR LOCATION.
  14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-337; SER-379; SER-1670; SER-1710 AND SER-1803, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-337; SER-1710 AND SER-1733, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMLP1_YEAST
AccessioniPrimary (citable) accession number: Q02455
Secondary accession number(s): D6VXF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2710 molecules/cell in log phase SD medium.

Caution

1 Publication misquoted the gene name as 'MPL1'.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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