Reviewed, UniProtKB/Swiss-Prot Q02446 (SP4_HUMAN)

Names and origin

Protein names

Recommended name:
    Transcription factor Sp4
Alternative name(s):
    SPR-1

Gene names

Name:

SP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	784 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to GT and GC boxes promoters elements. Probable transcriptional activator.
Subcellular location	Nucleus.
Tissue specificity	Abundant in brain.
Sequence similarities	Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II transcription factor activity Traceable author statement. Source: ProtInc transcription coactivator activity Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 784	784	Transcription factor Sp4		PRO_0000047144
Regions
	Zinc finger	647 – 671	25	C2H2-type 1
	Zinc finger	677 – 701	25	C2H2-type 2
	Zinc finger	707 – 729	23	C2H2-type 3
	Compositional bias	7 – 11	5	Poly-Glu
	Compositional bias	12 – 19	8	Poly-Ala
	Compositional bias	122 – 130	9	Poly-Ser
	Compositional bias	185 – 188	4	Poly-Ser
Amino acid modifications
	Modified residue	691	1	Phosphoserine Ref.6
Natural variations
	Natural variant	197	1	Q → K: dbSNP rs1042848. Ref.1		VAR_047975
Experimental info
	Sequence conflict	379 – 380	2	QP → HA in CAA48563. Ref.1
	Sequence conflict	386	1	A → Q in CAA48563. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q02446-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 5B0B03EC03657993	FASTA	784	81,985
	10 20 30 40 50 60 MSDQKKEEEE EAAAAAAMAT EGGKTSEPEN NNKKPKTSGS QDSQPSPLAL LAATCSKIGT 70 80 90 100 110 120 PGENQATGQQ QIIIDPSQGL VQLQNQPQQL ELVTTQLAGN AWQLVASTPP ASKENNVSQP 130 140 150 160 170 180 ASSSSSSSSS NNGSASPTKT KSGNSSTPGQ FQVIQVQNPS GSVQYQVIPQ LQTVEGQQIQ 190 200 210 220 230 240 INPTSSSSLQ DLQGQIQLIS AGNNQAILTA ANRTASGNIL AQNLANQTVP VQIRPGVSIP 250 260 270 280 290 300 LQLQTLPGTQ AQVVTTLPIN IGGVTLALPV INNVAAGGGT GQVGQPAATA DSGTSNGNQL 310 320 330 340 350 360 VSTPTNTTTS ASTMPESPSS STTCTTTAST SLTSSDTLVS SADTGQYAST SASSSERTIE 370 380 390 400 410 420 ESQTPAATES EAQSSSQLQP NGMQNAQDQS NSLQQVQIVG QPILQQIQIQ QPQQQIIQAI 430 440 450 460 470 480 PPQSFQLQSG QTIQTIQQQP LQNVQLQAVN PTQVLIRAPT LTPSGQISWQ TVQVQNIQSL 490 500 510 520 530 540 SNLQVQNAGL SQQLTITPVS SSGGTTLAQI APVAVAGAPI TLNTAQLASV PNLQTVSVAN 550 560 570 580 590 600 LGAAGVQVQG VPVTITSVAG QQQGQDGVKV QQATIAPVTV AVGGIANATI GAVSPDQLTQ 610 620 630 640 650 660 VHLQQGQQTS DQEVQPGKRL RRVACSCPNC REGEGRGSNE PGKKKQHICH IEGCGKVYGK 670 680 690 700 710 720 TSHLRAHLRW HTGERPFICN WMFCGKRFTR SDELQRHRRT HTGEKRFECP ECSKRFMRSD 730 740 750 760 770 780 HLSKHVKTHQ NKKGGGTALA IVTSGELDSS VTEVLGSPRI VTVAAISQDS NPATPNVSTN MEEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes." Hagen G., Mueller S., Beato M., Suske G. Nucleic Acids Res. 20:5519-5525(1992) [PubMed: 1454515] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-197. Tissue: Uterus.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-691, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X68561 mRNA. Translation: CAA48563.1. AK289728 mRNA. Translation: BAF82417.1. AC004595 Genomic DNA. No translation available. CH471073 Genomic DNA. Translation: EAW93733.1. BC109300 mRNA. Translation: AAI09301.1.
IPI	IPI00025805.
PIR	S26638.
RefSeq	NP_003103.2.
UniGene	Hs.88013
3D structure databases
HSSP	HSSP built from PDB template 1SP1 based on UniProtKB P08047.
ModBase	Search...
PTM databases
PhosphoSite	Q02446.
Genome annotation databases
Ensembl	ENSG00000105866. Homo sapiens. [Contig view]
GeneID	6671.
KEGG	hsa:6671.
Organism-specific databases
GeneCards	GC07P021434.
H-InvDB	HIX0033690.
HGNC	HGNC:11209. SP4.
HPA	CAB010246.
MIM	600540. gene.
PharmGKB	PA25069.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q02446.
HOVERGEN	Q02446.
OMA	Q02446. QLQSNGI.
Gene expression databases
ArrayExpress	Q02446.
Bgee	Q02446.
CleanEx	HS_SP4.
GermOnline	ENSG00000105866. Homo sapiens.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 2 hits.
Pfam	PF00096. zf-C2H2. 3 hits. [Graphical view]
ProDom	PD000003. Znf_C2H2. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00355. ZnF_C2H2. 3 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	26011.
SOURCE	Search...

Entry information

Entry name

SP4_HUMAN

Accession

Primary (citable) accession number: Q02446
Secondary accession number(s): O60402, Q32M52

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 16, 2008
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)