Reviewed, UniProtKB/Swiss-Prot Q02439 (E13F_HORVU)

Names and origin

Protein names	Recommended name:     Putative glucan endo-1,3-beta-glucosidase GVI     EC=3.2.1.39 Alternative name(s):     (1->3)-beta-glucan endohydrolase GVI     (1->3)-beta-glucanase isoenzyme GVI     Beta-1,3-endoglucanase GVI
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	321 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May provide a degree of protection against microbial invasion of germinated barley grain through its ability to degrade fungal cell wall polysaccharides.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 6	›6	By similarity
	Chain	7 – 321	315	Putative glucan endo-1,3-beta-glucosidase GVI		PRO_0000011850
Sites
	Active site	241	1	Nucleophile By similarity
	Active site	298	1	Proton donor By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q02439-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: D95BC94BF91573D9	FASTA	321	33,344
	10 20 30 40 50 60 LAGVEGIGVN YGMMGSDLPS PDKVVALYKA NNITDVRIFH PDTNVLEALR NSGLGVVLGT 70 80 90 100 110 120 LNSDLAPLAS DASYAASWVH SYVQPFAGAV SFRYINAGNE VIPGESAALV LPAMKNLEAA 130 140 150 160 170 180 LQAAGLSVPV TTAMATSVLG TSYPPSQGTF SEAALPTVGP IVSHLASSGT PLLVNVYPYF 190 200 210 220 230 240 AYSADPSSVR LDYALLSSSA AVAVTDNGVE YANMFDAILD AVYAAVEKAG GGESLELVVS 250 260 270 280 290 300 ETGWPSGGGG YGASVENAAA YINNLVRHVG GTPRRPGKAV ETYIFAMFNE NQKPEGVEQN 310 320 FGMFQPDMSQ VYHVDFTASS S

References

[1]

"Evolution and differential expression of the (1-->3)-beta-glucan endohydrolase-encoding gene family in barley, Hordeum vulgare." Xu P., Wang J., Fincher G.B. Gene 120:157-165(1992) [PubMed: 1398132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Clipper and cv. NK 1558. Tissue: Seedling.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96941 Genomic DNA. Translation: AAA32957.1.
PIR	JC1439.
3D structure databases
SMR	Q02439. Positions 7-316.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Organism-specific databases
Gramene	Q02439.
Enzyme and pathway databases
BRENDA	3.2.1.39. 283.
Gene expression databases
Genevestigator	Q02439.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13F_HORVU

Accession

Primary (citable) accession number: Q02439

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 19, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)