Reviewed, UniProtKB/Swiss-Prot Q02438 (E13E_HORVU)

Names and origin

Protein names	Recommended name:     Glucan endo-1,3-beta-glucosidase GV     EC=3.2.1.39 Alternative name(s):     (1->3)-beta-glucan endohydrolase GV     (1->3)-beta-glucanase isoenzyme GV     Beta-1,3-endoglucanase GV
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May provide a degree of protection against microbial invasion of germinated barley grain through its ability to degrade fungal cell wall polysaccharides.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Glucan endo-1,3-beta-glucosidase GV		PRO_0000205275
Sites
	Active site	239	1	Nucleophile By similarity
	Active site	296	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02438-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 0CC0AA9D48269B4F	FASTA	316	34,414
	10 20 30 40 50 60 MGAVHGVCYG MVGDNLPSRS DVVQLYKSRN IHAMRIYNPD QEALTALRGS GIFLILDVGG 70 80 90 100 110 120 VDEVRRLGRD PSYAAGWVRS NVQAYYPDVL IRYIAVGNEV PAGDTGIILL AMQNVHNALA 130 140 150 160 170 180 SANLSSSIKV STAVRFDVIT NSFPPSSGVF RDPSGLVPIA RFLDSTGAPF LANVYPYFAY 190 200 210 220 230 240 RDDRGQNIRL NYATLQPGTT VRDNGNGLTY TSLFDAMVDS IYAALEKAGT PNVRVVVSES 250 260 270 280 290 300 GWPSAGGFGA SVENARNYNQ GLIDHIRSGT PKRPGAIETY IFAMFNENRK PGDEVERNFG 310 LFFPNKQPVY PTTFPN

References

[1]	"Heterologous expression of cDNAs encoding barley (Hordeum vulgare) (1-->3)-beta-glucanase isoenzyme GV." Xu P., Harvey A.J., Fincher G.B. FEBS Lett. 348:206-210(1994) [PubMed: 8034043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Clipper. Tissue: Leaf and Root.
[2]	"Evolution and differential expression of the (1-->3)-beta-glucan endohydrolase-encoding gene family in barley, Hordeum vulgare." Xu P., Wang J., Fincher G.B. Gene 120:157-165(1992) [PubMed: 1398132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-316. Strain: cv. Clipper. Tissue: Leaf and Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M96939 mRNA. Translation: AAA21564.1.
PIR	S46237.
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Organism-specific databases
Gramene	Q02438.
Enzyme and pathway databases
BRENDA	3.2.1.39. 283.
Gene expression databases
Genevestigator	Q02438.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13E_HORVU

Accession

Primary (citable) accession number: Q02438

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	February 1, 1996
Last modified:	October 13, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)