Q02437 (E13D_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glucan endo-1,3-beta-glucosidase GIV EC=3.2.1.39 Alternative name(s): (1->3)-beta-glucan endohydrolase GIV (1->3)-beta-glucanase isoenzyme GIV Beta-1,3-endoglucanase GIV
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May provide a degree of protection against microbial invasion of germinated barley grain through its ability to degrade fungal cell wall polysaccharides.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Vacuole Probable.
Tissue specificity	Aleurone layer of germinated grain.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Vacuole
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Glucan endo-1,3-beta-glucosidase GIV		PRO_0000205274
Sites
	Active site	233	1	Nucleophile By similarity
	Active site	290	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	317	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q02437 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: C5002AC7AB830E85	FASTA	327	35,034
	10 20 30 40 50 60 IGVCYGIIAN NLPPRREVVQ LYRSKGITNM RIYSVQPQAI RALHGSGIRL MLGTTNNDVA 70 80 90 100 110 120 VLAGSLSAAT SWVHANVKPY HSAGVTIRYI AVGNEITGGA AQSILAAMRN LNKALAAARL 130 140 150 160 170 180 GGIKVSTAVR FDVITNSFPP SSAVFAQPYM VDIARHLAST NAPLLANVYP YFAYSGNPRD 190 200 210 220 230 240 IKLNYATFQP GATPVRDAGN GLIYTNLFNA MVDAMYAALE KAGAPSVRVV VSESGWPSAG 250 260 270 280 290 300 GFAATPENAR AYNQGLIDHV AHGTPKKPGH MEAYVFAMFN ENQKPGLETE RHFGLFYPNK 310 320 RPVYHINFAG GRLAPVNHTN SHGFGGH

References

[1]

"Evolution and differential expression of the (1-->3)-beta-glucan endohydrolase-encoding gene family in barley, Hordeum vulgare." Xu P., Wang J., Fincher G.B. Gene 120:157-165(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Clipper and cv. NK 1558. Tissue: Seedling.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96940 Genomic DNA. Translation: AAA32961.1.
PIR	JC1437.
3D structure databases
ProteinModelPortal	Q02437.
SMR	Q02437. Positions 1-308.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q02437.
Gene expression databases
Genevestigator	Q02437.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13D_HORVU

Accession

Primary (citable) accession number: Q02437

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program