Q02427 (RBP1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-binding protein 1

Gene names

Name:	Rbp1
Synonyms:	Rbp11
ORF Names:	CG17136

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Contributes to the activation of female-specific DSX splicing in vivo by recognizing the RBP1 target sequences within the purine-rich polypyrimidine tract of the female-specific 3' splice site. Ref.1 Ref.6
Subcellular location	Nucleus Ref.1.
Tissue specificity	Ubiquitous. Ref.1
Developmental stage	Found at all developmental stages. Ref.1
Post-translational modification	Extensively phosphorylated on serine residues in the RS domain Probable.
Sequence similarities	Belongs to the splicing factor SR family. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA splicing Inferred from mutant phenotype. Source: FlyBase mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay Ref.1. Source: FlyBase
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform D (identifier: Q02427-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform B (identifier: Q02427-2)

Also known as: RBP1-B;

The sequence of this isoform differs from the canonical sequence as follows:
     107-144: Missing.

Isoform A (identifier: Q02427-1)

Also known as: RBP1-A; C;

The sequence of this isoform differs from the canonical sequence as follows:
     107-144: ITPSARTTSTATSSFYNINNLQQQPSSQPQPATFNLQL → SRSPRRSRSPRSRSFSRDRRSRSDSRDRH

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 144	144	RNA-binding protein 1		PRO_0000081797
Regions
	Domain	11 – 84	74	RRM
	Compositional bias	82 – 133	52	Arg/Ser-rich (RS domain)
Natural variations
	Alternative sequence	107 – 144	38	ITPSA…FNLQL → SRSPRRSRSPRSRSFSRDRR SRSDSRDRH in isoform A.		VSP_011815
	Alternative sequence	107 – 144	38	Missing in isoform B.		VSP_005817
Experimental info
	Sequence conflict	14	1	Y → F in AAB24622. Ref.5
	Sequence conflict	14	1	Y → F in AAB24631. Ref.5
	Sequence conflict	19 – 29	11	GSSASKHEIEG → APRRSKPRDRS in AAB24631. Ref.5
	Sequence conflict	65	1	R → A in AAA28850. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform D [UniParc]. Last modified November 9, 2004. Version 3. Checksum: AB5B6D782CED5A46	FASTA	144	16,013
	10 20 30 40 50 60 MPRYREWDLA CKVYVGNLGS SASKHEIEGA FAKYGPLRNV WVARNPPGFA FVEFEDRRDA 70 80 90 100 110 120 EDATRALDGT RCCGTRIRVE MSSGRSRDRR RGEGGSSGRS GSGRYRITPS ARTTSTATSS 130 140 FYNINNLQQQ PSSQPQPATF NLQL
	Isoform B (RBP1-B) [UniParc]. Checksum: 6A033D384DD71F8F	FASTA	106	11,877
	10 20 30 40 50 60 MPRYREWDLA CKVYVGNLGS SASKHEIEGA FAKYGPLRNV WVARNPPGFA FVEFEDRRDA 70 80 90 100 EDATRALDGT RCCGTRIRVE MSSGRSRDRR RGEGGSSGRS GSGRYR
	Isoform A (RBP1-A) (C) [UniParc]. Checksum: 896DCE902518D991	FASTA	135	15,446
	10 20 30 40 50 60 MPRYREWDLA CKVYVGNLGS SASKHEIEGA FAKYGPLRNV WVARNPPGFA FVEFEDRRDA 70 80 90 100 110 120 EDATRALDGT RCCGTRIRVE MSSGRSRDRR RGEGGSSGRS GSGRYRSRSP RRSRSPRSRS 130 FSRDRRSRSD SRDRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila RNA-binding protein RBP1 is localized to transcriptionally active sites of chromosomes and shows a functional similarity to human splicing factor ASF/SF2." Kim Y.-J., Zuo P., Manley J.L., Baker B.S. Genes Dev. 6:2569-2579(1992) [PubMed: 1340470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS A AND B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D). Strain: Berkeley. Tissue: Ovary.
[5]	"Isolation of RRM-type RNA-binding protein genes and the analysis of their relatedness by using a numerical approach." Kim Y.-J., Baker B.S. Mol. Cell. Biol. 13:174-183(1993) [PubMed: 8417324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-52 (ISOFORMS A/B/D).
[6]	"The Drosophila SR protein RBP1 contributes to the regulation of doublesex alternative splicing by recognizing RBP1 RNA target sequences." Heinrichs V., Baker B.S. EMBO J. 14:3987-4000(1995) [PubMed: 7664738] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L04929 Genomic DNA. Translation: AAA28850.1. AE014297 Genomic DNA. Translation: AAF54555.1. AE014297 Genomic DNA. Translation: AAN13487.1. AE014297 Genomic DNA. Translation: AAN13488.1. AE014297 Genomic DNA. Translation: AAN13486.1. AY069252 mRNA. Translation: AAL39397.1. S51691 mRNA. Translation: AAB24622.1. S51740 mRNA. Translation: AAB24631.1.
PIR	A46398. A47752. A48110.
RefSeq	NP_524307.1. NM_079583.1. NP_731509.1. NM_169363.1. NP_731510.1. NM_169364.1. NP_731511.1. NM_169365.1.
UniGene	Dm.2213.
3D structure databases
ProteinModelPortal	Q02427.
SMR	Q02427. Positions 9-87.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q02427. 6 interactions.
MINT	MINT-277307.
STRING	Q02427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0082277; FBpp0081754; FBgn0260944.
GeneID	41294.
KEGG	dme:Dmel_CG17136.
Organism-specific databases
CTD	5947.
FlyBase	FBgn0260944. Rbp1.
Phylogenomic databases
eggNOG	inNOG10932.
GeneTree	EMGT00050000000447.
InParanoid	Q02427.
OMA	DNGYRRS.
OrthoDB	EOG4CNP7N.
PhylomeDB	Q02427.
Gene expression databases
ArrayExpress	Q02427.
Bgee	Q02427.
GermOnline	CG17136. Drosophila melanogaster.
Family and domain databases
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	823159.

Entry information

Entry name

RBP1_DROME

Accession

Primary (citable) accession number: Q02427
Secondary accession number(s): A4V2P0 , Q26271, Q26280, Q8T9K6, Q9VGW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 9, 2004
Last modified:	January 25, 2012
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project