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Q02401

- LPH_RAT

UniProt

Q02401 - LPH_RAT

Protein

Lactase-phlorizin hydrolase

Gene

Lct

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    LPH splits lactose in the small intestine.

    Catalytic activityi

    Lactose + H2O = D-galactose + D-glucose.
    Glycosyl-N-acylsphingosine + H2O = N-acylsphingosine + a sugar.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei1067 – 10671Proton donorSequence Analysis
    Active sitei1274 – 12741NucleophilePROSITE-ProRule annotation
    Active sitei1539 – 15391Proton donorSequence Analysis
    Active sitei1750 – 17501NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. glycosylceramidase activity Source: UniProtKB-EC
    2. lactase activity Source: RGD
    3. transferase activity Source: RGD

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. response to drug Source: RGD
    3. response to estrogen Source: RGD
    4. response to ethanol Source: RGD
    5. response to hormone Source: RGD
    6. response to hypoxia Source: RGD
    7. response to iron(II) ion Source: RGD
    8. response to lead ion Source: RGD
    9. response to nickel cation Source: RGD
    10. response to nutrient Source: RGD
    11. response to organic substance Source: RGD
    12. response to starvation Source: RGD
    13. response to sucrose Source: RGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lactase-phlorizin hydrolase
    Alternative name(s):
    Lactase-glycosylceramidase
    Including the following 2 domains:
    Phlorizin hydrolase (EC:3.2.1.62)
    Gene namesi
    Name:Lct
    Synonyms:Lph
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620823. Lct.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. brush border Source: RGD
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919By similarityAdd
    BLAST
    Propeptidei20 – 867848Beta-glucosidasePRO_0000011771Add
    BLAST
    Chaini868 – 19281061Lactase-phlorizin hydrolasePRO_0000011772Add
    BLAST

    Keywords - PTMi

    Zymogen

    Proteomic databases

    PaxDbiQ02401.
    PRIDEiQ02401.

    PTM databases

    PhosphoSiteiQ02401.

    Expressioni

    Tissue specificityi

    Intestine.

    Gene expression databases

    GenevestigatoriQ02401.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliQ02401.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 18831862ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1903 – 192826CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1884 – 190219HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati89 – 175871Add
    BLAST
    Repeati365 – 8494852Add
    BLAST
    Repeati886 – 13664813Add
    BLAST
    Repeati1371 – 18424724Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni89 – 184217544 X approximate repeatsAdd
    BLAST

    Domaini

    The sequence exhibits 4 regions (I-IV) of internal homology; therefore LPH might have evolved by two cycles of partial gene duplication.

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2723.
    HOVERGENiHBG006290.
    InParanoidiQ02401.
    PhylomeDBiQ02401.

    Family and domain databases

    Gene3Di3.20.20.80. 4 hits.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 4 hits.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 4 hits.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q02401-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELPWTALFL STVLLGLSCQ GSDWESDRNF ISAAGPLTND LVLNLNYPPG     50
    KQGSDVVSGN TDHLLCQQPL PSFLSQYFSS LRASQVTHYK VLLSWAQLLP 100
    TGSSKNPDQE AVQCYRQLLQ SLKDAQLEPM VVLCHQTPPT SSAIQREGAF 150
    ADLFADYATL AFQSFGDLVE IWFTFSDLEK VIMDLPHKDL KASALQTLSN 200
    AHRRAFEIYH RKFSSQGGKL SVVLKAEDIP ELLPDPALAA LVQGSVDFLS 250
    LDLSYECQSV ATLPQKLSEL QNLEPKVKVF IYTLKLEDCP ATGTSPSSLL 300
    ISLLEAINKD QIQTVGFDVN AFLSCTSNSE ESPSCSLTDS LALQTEQQQE 350
    TAVPSSPGSA YQRVWAAFAN QSREERDAFL QDVFPEGFLW GISTGAFNVE 400
    GGWAEGGRGP SIWDHYGNLN AAEGQATAKV ASDSYHKPAS DVALLRGIRA 450
    QVYKFSISWS GLFPLGQKST PNRQGVAYYN KLIDRLLDSH IEPMATLFHW 500
    DLPQALQEQG GWQNESVVEA FLDYAAFCFS TFGDRVKLWV TFHEPWVMSY 550
    AGYGTGQHAP AISDPGMASF KVAHLILKAH ARTWHLYDLH HRLQQQGRVG 600
    IVLNSDLAEP LDRKSPQDLA AAERFLHFML GWFAHPIFVD GDYPTTSAQI 650
    QHINQQCGHP LAQLPEFTEA EKRLLKGSAD FLGLSHYTSR LISKAGRQTC 700
    TSSYDNIGGF SQHVDPEWPQ TASPWIRVVP WGIRRLLRFA SMEYTKGKLP 750
    IFLAGNGMPV GEEADLFDDS VRVNYFNWYI NEVLKAVKED LVDVRSYIVR 800
    SLIDGYEGPL GFSQRFGLYH VNFNDSSRPR TPRKSAYLFT SIIEKNGFSA 850
    KKVKRNPLPV RADFTSRARV TDSLPSEVPS KAKISVEKFS KQPRFERDLF 900
    YDGRFRDDFL WGVSSSPYQI EGGWNADGKG PSIWDNFTHT PGNGVKDNAT 950
    GDVACDSYHQ LDADLNILRT LKVKSYRFSI SWSRIFPTGR NSTINKQGVD 1000
    YYNRLIDSLV DNNIFPMVTL FHWDLPQALQ DIGGWENPSL IELFDSYADY 1050
    CFKTFGDRVK FWMTFNEPWC HVVLGYSSGI FPPSVQEPGW LPYKVSHIVI 1100
    KAHARVYHTY DEKYRSEQKG VISLSLNTHW AEPKDPGLQR DVEAADRMLQ 1150
    FTMGWFAHPI FKNGDYPDVM KWTVGNRSEL QHLASSRLPT FTEEEKNYVR 1200
    GTADVFCHNT YTSVFVQHST PRLNPPSYDD DMELKLIEMN SSTGVMHQDV 1250
    PWGTRRLLNW IKEEYGNIPI YITENGQGLE NPTLDDTERI FYHKTYINEA 1300
    LKAYKLDGVD LRGYSAWTLM DDFEWLLGYT MRFGLYYVDF NHVSRPRTAR 1350
    ASARYYPDLI ANNGMPLARE DEFLYGEFPK GFIWSAASAS YQVEGAWRAD 1400
    GKGLSIWDTF SHTPLRIGND DNGDVACDSY HKIAEDVVAL QNLGVSHYRF 1450
    SIAWSRILPD GTTKFINEAG LSYYVRFIDA LLAAGITPQV TIYHWDLPQA 1500
    LQDVGGWENE TIVQRFKEYA DVLFQRLGDR VKFWITLNEP FVIAAQGYGT 1550
    GVSAPGISFR PGTAPYIAGH NLIKAHAEAW HLYNDVYRAR QGGTISITIS 1600
    SDWGEPRDPT NREHVEAARS YVQFMGGWFA HPIFKNGDYP EVMKTRIRDR 1650
    SLGAGLNKSR LPEFTESEKS RIKGTFDFFG FNHNTTVLAY NLDYPAAFSS 1700
    FDADRGVASI ADSSWPVSGS FWLKVTPFGF RRILNWLKEE YNNPPIYVTE 1750
    NGVSRRGEPE LNDTDRIYYL RSYINEALKA VHDKVDLRGY TVWSIMDNFE 1800
    WATGFAERFG VHFVNRSDPS LPRIPRASAK FYATIVRCNG FPDPAQGPHP 1850
    CLQQPEDAAP TASPVQSEVP FLGLMLGIAE AQTALYVLFA LLLLGACSLA 1900
    FLTYNTGRRS KQGNAQPSQH QLSPISSF 1928
    Length:1,928
    Mass (Da):217,268
    Last modified:May 30, 2000 - v2
    Checksum:i56DDCAAC4ACAEA85
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71A → R in AAA41539. (PubMed:1909681)Curated
    Sequence conflicti113 – 1131Q → E in AAA41539. (PubMed:1909681)Curated
    Sequence conflicti207 – 2071E → D in AAA41539. (PubMed:1909681)Curated
    Sequence conflicti1337 – 13371Y → H(PubMed:1691182)Curated
    Sequence conflicti1357 – 13593PDL → AEV(PubMed:1691182)Curated
    Sequence conflicti1369 – 13702RE → GK(PubMed:1691182)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56748 Genomic DNA. Translation: CAA40070.1.
    X56747 mRNA. Translation: CAA40069.1.
    L04635 Genomic DNA. Translation: AAA41539.1.
    PIRiJS0610.
    UniGeneiRn.92376.

    Genome annotation databases

    UCSCiRGD:620823. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56748 Genomic DNA. Translation: CAA40070.1 .
    X56747 mRNA. Translation: CAA40069.1 .
    L04635 Genomic DNA. Translation: AAA41539.1 .
    PIRi JS0610.
    UniGenei Rn.92376.

    3D structure databases

    ProteinModelPortali Q02401.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q02401.
    ChEMBLi CHEMBL3389.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    PTM databases

    PhosphoSitei Q02401.

    Proteomic databases

    PaxDbi Q02401.
    PRIDEi Q02401.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:620823. rat.

    Organism-specific databases

    RGDi 620823. Lct.

    Phylogenomic databases

    eggNOGi COG2723.
    HOVERGENi HBG006290.
    InParanoidi Q02401.
    PhylomeDBi Q02401.

    Miscellaneous databases

    PROi Q02401.

    Gene expression databases

    Genevestigatori Q02401.

    Family and domain databases

    Gene3Di 3.20.20.80. 4 hits.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 4 hits.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 4 hits.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the precursor of intestinal lactase-phlorizin hydrolase from fetal rat."
      Duluc I., Boukamel R., Mantei N., Semenza G., Raul F., Freund J.-N.
      Gene 103:275-276(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Wistar.
      Tissue: Intestine.
    2. "The rat LPH gene 5' region: comparative structure with the human gene."
      Boukamel R., Freund J.-N.
      DNA Seq. 3:119-121(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192.
      Strain: Sprague-Dawley.
    3. "Coordinate expression of lactase-phlorizin hydrolase mRNA and enzyme levels in rat intestine during development."
      Bueller H.A., Kothe M.J., Goldman D.A., Grubman S.A., Sasak W.V., Matsudaira P.T., Montgomery R.K., Grand R.J.
      J. Biol. Chem. 265:6978-6983(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1288-1370.

    Entry informationi

    Entry nameiLPH_RAT
    AccessioniPrimary (citable) accession number: Q02401
    Secondary accession number(s): Q63712, Q63719
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3