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Q02401

- LPH_RAT

UniProt

Q02401 - LPH_RAT

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Protein
Lactase-phlorizin hydrolase
Gene
Lct, Lph
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

LPH splits lactose in the small intestine.

Catalytic activityi

Lactose + H2O = D-galactose + D-glucose.
Glycosyl-N-acylsphingosine + H2O = N-acylsphingosine + a sugar.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1067 – 10671Proton donor Reviewed prediction
Active sitei1274 – 12741Nucleophile By similarity
Active sitei1539 – 15391Proton donor Reviewed prediction
Active sitei1750 – 17501Nucleophile By similarity

GO - Molecular functioni

  1. glycosylceramidase activity Source: UniProtKB-EC
  2. lactase activity Source: RGD
  3. transferase activity Source: RGD

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. response to drug Source: RGD
  3. response to estrogen Source: RGD
  4. response to ethanol Source: RGD
  5. response to hormone Source: RGD
  6. response to hypoxia Source: RGD
  7. response to iron(II) ion Source: RGD
  8. response to lead ion Source: RGD
  9. response to nickel cation Source: RGD
  10. response to nutrient Source: RGD
  11. response to organic substance Source: RGD
  12. response to starvation Source: RGD
  13. response to sucrose Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactase-phlorizin hydrolase
Alternative name(s):
Lactase-glycosylceramidase
Including the following 2 domains:
Phlorizin hydrolase (EC:3.2.1.62)
Gene namesi
Name:Lct
Synonyms:Lph
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620823. Lct.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 18831862Extracellular Reviewed prediction
Add
BLAST
Transmembranei1884 – 190219Helical; Reviewed prediction
Add
BLAST
Topological domaini1903 – 192826Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. brush border Source: RGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 By similarity
Add
BLAST
Propeptidei20 – 867848Beta-glucosidase
PRO_0000011771Add
BLAST
Chaini868 – 19281061Lactase-phlorizin hydrolase
PRO_0000011772Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiQ02401.
PRIDEiQ02401.

PTM databases

PhosphoSiteiQ02401.

Expressioni

Tissue specificityi

Intestine.

Gene expression databases

GenevestigatoriQ02401.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ02401.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati89 – 175871
Add
BLAST
Repeati365 – 8494852
Add
BLAST
Repeati886 – 13664813
Add
BLAST
Repeati1371 – 18424724
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni89 – 184217544 X approximate repeats
Add
BLAST

Domaini

The sequence exhibits 4 regions (I-IV) of internal homology; therefore LPH might have evolved by two cycles of partial gene duplication.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2723.
HOVERGENiHBG006290.
InParanoidiQ02401.
PhylomeDBiQ02401.

Family and domain databases

Gene3Di3.20.20.80. 4 hits.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 4 hits.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 4 hits.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02401-1 [UniParc]FASTAAdd to Basket

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MELPWTALFL STVLLGLSCQ GSDWESDRNF ISAAGPLTND LVLNLNYPPG     50
KQGSDVVSGN TDHLLCQQPL PSFLSQYFSS LRASQVTHYK VLLSWAQLLP 100
TGSSKNPDQE AVQCYRQLLQ SLKDAQLEPM VVLCHQTPPT SSAIQREGAF 150
ADLFADYATL AFQSFGDLVE IWFTFSDLEK VIMDLPHKDL KASALQTLSN 200
AHRRAFEIYH RKFSSQGGKL SVVLKAEDIP ELLPDPALAA LVQGSVDFLS 250
LDLSYECQSV ATLPQKLSEL QNLEPKVKVF IYTLKLEDCP ATGTSPSSLL 300
ISLLEAINKD QIQTVGFDVN AFLSCTSNSE ESPSCSLTDS LALQTEQQQE 350
TAVPSSPGSA YQRVWAAFAN QSREERDAFL QDVFPEGFLW GISTGAFNVE 400
GGWAEGGRGP SIWDHYGNLN AAEGQATAKV ASDSYHKPAS DVALLRGIRA 450
QVYKFSISWS GLFPLGQKST PNRQGVAYYN KLIDRLLDSH IEPMATLFHW 500
DLPQALQEQG GWQNESVVEA FLDYAAFCFS TFGDRVKLWV TFHEPWVMSY 550
AGYGTGQHAP AISDPGMASF KVAHLILKAH ARTWHLYDLH HRLQQQGRVG 600
IVLNSDLAEP LDRKSPQDLA AAERFLHFML GWFAHPIFVD GDYPTTSAQI 650
QHINQQCGHP LAQLPEFTEA EKRLLKGSAD FLGLSHYTSR LISKAGRQTC 700
TSSYDNIGGF SQHVDPEWPQ TASPWIRVVP WGIRRLLRFA SMEYTKGKLP 750
IFLAGNGMPV GEEADLFDDS VRVNYFNWYI NEVLKAVKED LVDVRSYIVR 800
SLIDGYEGPL GFSQRFGLYH VNFNDSSRPR TPRKSAYLFT SIIEKNGFSA 850
KKVKRNPLPV RADFTSRARV TDSLPSEVPS KAKISVEKFS KQPRFERDLF 900
YDGRFRDDFL WGVSSSPYQI EGGWNADGKG PSIWDNFTHT PGNGVKDNAT 950
GDVACDSYHQ LDADLNILRT LKVKSYRFSI SWSRIFPTGR NSTINKQGVD 1000
YYNRLIDSLV DNNIFPMVTL FHWDLPQALQ DIGGWENPSL IELFDSYADY 1050
CFKTFGDRVK FWMTFNEPWC HVVLGYSSGI FPPSVQEPGW LPYKVSHIVI 1100
KAHARVYHTY DEKYRSEQKG VISLSLNTHW AEPKDPGLQR DVEAADRMLQ 1150
FTMGWFAHPI FKNGDYPDVM KWTVGNRSEL QHLASSRLPT FTEEEKNYVR 1200
GTADVFCHNT YTSVFVQHST PRLNPPSYDD DMELKLIEMN SSTGVMHQDV 1250
PWGTRRLLNW IKEEYGNIPI YITENGQGLE NPTLDDTERI FYHKTYINEA 1300
LKAYKLDGVD LRGYSAWTLM DDFEWLLGYT MRFGLYYVDF NHVSRPRTAR 1350
ASARYYPDLI ANNGMPLARE DEFLYGEFPK GFIWSAASAS YQVEGAWRAD 1400
GKGLSIWDTF SHTPLRIGND DNGDVACDSY HKIAEDVVAL QNLGVSHYRF 1450
SIAWSRILPD GTTKFINEAG LSYYVRFIDA LLAAGITPQV TIYHWDLPQA 1500
LQDVGGWENE TIVQRFKEYA DVLFQRLGDR VKFWITLNEP FVIAAQGYGT 1550
GVSAPGISFR PGTAPYIAGH NLIKAHAEAW HLYNDVYRAR QGGTISITIS 1600
SDWGEPRDPT NREHVEAARS YVQFMGGWFA HPIFKNGDYP EVMKTRIRDR 1650
SLGAGLNKSR LPEFTESEKS RIKGTFDFFG FNHNTTVLAY NLDYPAAFSS 1700
FDADRGVASI ADSSWPVSGS FWLKVTPFGF RRILNWLKEE YNNPPIYVTE 1750
NGVSRRGEPE LNDTDRIYYL RSYINEALKA VHDKVDLRGY TVWSIMDNFE 1800
WATGFAERFG VHFVNRSDPS LPRIPRASAK FYATIVRCNG FPDPAQGPHP 1850
CLQQPEDAAP TASPVQSEVP FLGLMLGIAE AQTALYVLFA LLLLGACSLA 1900
FLTYNTGRRS KQGNAQPSQH QLSPISSF 1928
Length:1,928
Mass (Da):217,268
Last modified:May 30, 2000 - v2
Checksum:i56DDCAAC4ACAEA85
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71A → R in AAA41539. 1 Publication
Sequence conflicti113 – 1131Q → E in AAA41539. 1 Publication
Sequence conflicti207 – 2071E → D in AAA41539. 1 Publication
Sequence conflicti1337 – 13371Y → H1 Publication
Sequence conflicti1357 – 13593PDL → AEV1 Publication
Sequence conflicti1369 – 13702RE → GK1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56748 Genomic DNA. Translation: CAA40070.1.
X56747 mRNA. Translation: CAA40069.1.
L04635 Genomic DNA. Translation: AAA41539.1.
PIRiJS0610.
UniGeneiRn.92376.

Genome annotation databases

UCSCiRGD:620823. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56748 Genomic DNA. Translation: CAA40070.1 .
X56747 mRNA. Translation: CAA40069.1 .
L04635 Genomic DNA. Translation: AAA41539.1 .
PIRi JS0610.
UniGenei Rn.92376.

3D structure databases

ProteinModelPortali Q02401.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q02401.
ChEMBLi CHEMBL3389.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

PTM databases

PhosphoSitei Q02401.

Proteomic databases

PaxDbi Q02401.
PRIDEi Q02401.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:620823. rat.

Organism-specific databases

RGDi 620823. Lct.

Phylogenomic databases

eggNOGi COG2723.
HOVERGENi HBG006290.
InParanoidi Q02401.
PhylomeDBi Q02401.

Miscellaneous databases

PROi Q02401.

Gene expression databases

Genevestigatori Q02401.

Family and domain databases

Gene3Di 3.20.20.80. 4 hits.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 4 hits.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 4 hits.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the precursor of intestinal lactase-phlorizin hydrolase from fetal rat."
    Duluc I., Boukamel R., Mantei N., Semenza G., Raul F., Freund J.-N.
    Gene 103:275-276(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Wistar.
    Tissue: Intestine.
  2. "The rat LPH gene 5' region: comparative structure with the human gene."
    Boukamel R., Freund J.-N.
    DNA Seq. 3:119-121(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192.
    Strain: Sprague-Dawley.
  3. "Coordinate expression of lactase-phlorizin hydrolase mRNA and enzyme levels in rat intestine during development."
    Bueller H.A., Kothe M.J., Goldman D.A., Grubman S.A., Sasak W.V., Matsudaira P.T., Montgomery R.K., Grand R.J.
    J. Biol. Chem. 265:6978-6983(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1288-1370.

Entry informationi

Entry nameiLPH_RAT
AccessioniPrimary (citable) accession number: Q02401
Secondary accession number(s): Q63712, Q63719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 30, 2000
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi