Reviewed,
UniProtKB/Swiss-Prot Q02401 (LPH_RAT)
Last modified
June 16, 2009.
Version 85.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Lactase-phlorizin hydrolase Alternative name(s): Lactase-glycosylceramidase Including the following 2 domains: 1- Recommended name: Lactase EC=3.2.1.108 2- Recommended name: Phlorizin hydrolase EC=3.2.1.62 | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 1928 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | LPH splits lactose in the small intestine. |
| Catalytic activity | Lactose + H2O = D-galactose + D-glucose. Glycosyl-N-acylsphingosine + H2O = N-acylsphingosine + a sugar. |
| Subcellular location | Apical cell membrane; Single-pass type I membrane protein. Note: Brush border. |
| Tissue specificity | Intestine. |
| Domain | The sequence exhibits 4 regions (I-IV) of internal homology; therefore LPH might have evolved by two cycles of partial gene duplication. |
| Sequence similarities | Belongs to the glycosyl hydrolase 1 family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | By similarity | ||||||
| Propeptide | 20 – 867 | 848 | Beta-glucosidase | PRO_0000011771 | |||||
| Chain | 868 – 1928 | 1061 | Lactase-phlorizin hydrolase | PRO_0000011772 | |||||
Regions | |||||||||
| Topological domain | 22 – 1883 | 1862 | Extracellular Potential | ||||||
| Transmembrane | 1884 – 1902 | 19 | Potential | ||||||
| Topological domain | 1903 – 1928 | 26 | Cytoplasmic Potential | ||||||
| Repeat | 89 – 175 | 87 | 1 | ||||||
| Repeat | 365 – 849 | 485 | 2 | ||||||
| Repeat | 886 – 1366 | 481 | 3 | ||||||
| Repeat | 1371 – 1842 | 472 | 4 | ||||||
| Region | 89 – 1842 | 1754 | 4 X approximate repeats | ||||||
Sites | |||||||||
| Active site | 1067 | 1 | Proton donor Potential | ||||||
| Active site | 1274 | 1 | Nucleophile By similarity | ||||||
| Active site | 1539 | 1 | Proton donor Potential | ||||||
| Active site | 1750 | 1 | Nucleophile By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1304 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 1314 | 1 | Phosphotyrosine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 7 | 1 | A → R in AAA41539. Ref.1 | ||||||
| Sequence conflict | 113 | 1 | Q → E in AAA41539. Ref.1 | ||||||
| Sequence conflict | 207 | 1 | E → D in AAA41539. Ref.1 | ||||||
| Sequence conflict | 1337 | 1 | Y → H Ref.3 | ||||||
| Sequence conflict | 1357 – 1359 | 3 | PDL → AEV Ref.3 | ||||||
| Sequence conflict | 1369 – 1370 | 2 | RE → GK Ref.3 | ||||||
Sequences
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References
| [1] | "Sequence of the precursor of intestinal lactase-phlorizin hydrolase from fetal rat." Duluc I., Boukamel R., Mantei N., Semenza G., Raul F., Freund J.-N. Gene 103:275-276(1991) [PubMed: 1909681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar. Tissue: Intestine. |
| [2] | "The rat LPH gene 5' region: comparative structure with the human gene." Boukamel R., Freund J.-N. DNA Seq. 3:119-121(1992) [PubMed: 1339333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192. Strain: Sprague-Dawley. |
| [3] | "Coordinate expression of lactase-phlorizin hydrolase mRNA and enzyme levels in rat intestine during development." Bueller H.A., Kothe M.J., Goldman D.A., Grubman S.A., Sasak W.V., Matsudaira P.T., Montgomery R.K., Grand R.J. J. Biol. Chem. 265:6978-6983(1990) [PubMed: 1691182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1288-1370. |
Cross-references
Sequence databases | |
|---|---|
| X56748 Genomic DNA. Translation: CAA40070.1. X56747 mRNA. Translation: CAA40069.1. L04635 Genomic DNA. Translation: AAA41539.1. | |
| IPI | IPI00206884. |
| PIR | JS0610. |
| UniGene | Rn.92376 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1OD0 based on UniProtKB Q08638. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH1. Glycoside Hydrolase Family 1. |
Proteomic databases | |
| PRIDE | Q02401. |
Genome annotation databases | |
| Ensembl | ENSRNOG00000003681. Rattus norvegicus. [Contig view] |
Organism-specific databases | |
| RGD | 620823. Lct. |
Phylogenomic databases | |
| HOVERGEN | Q02401. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.108. 248. 3.2.1.62. 248. |
Gene expression databases | |
| ArrayExpress | Q02401. |
| GermOnline | ENSRNOG00000003681. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view] |
| Gene3D | G3DSA:3.20.20.80. Glyco_hydro_cat. 4 hits. |
| PANTHER | PTHR10353. Glyco_hydro_1. 1 hit. |
| Pfam | PF00232. Glyco_hydro_1. 3 hits. [Graphical view] |
| PRINTS | PR00131. GLHYDRLASE1. |
| ProDom | PD000650. Glyco_hydro_euk. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LPH_RAT | ||||||||
| Accession | Primary (citable) accession number: Q02401 Secondary accession number(s): Q63712, Q63719 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |
