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Q02401

- LPH_RAT

UniProt

Q02401 - LPH_RAT

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Protein

Lactase-phlorizin hydrolase

Gene

Lct

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

LPH splits lactose in the small intestine.

Catalytic activityi

Lactose + H2O = D-galactose + D-glucose.
Glycosyl-N-acylsphingosine + H2O = N-acylsphingosine + a sugar.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1067 – 10671Proton donorSequence Analysis
Active sitei1274 – 12741NucleophilePROSITE-ProRule annotation
Active sitei1539 – 15391Proton donorSequence Analysis
Active sitei1750 – 17501NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  1. glycosylceramidase activity Source: UniProtKB-EC
  2. lactase activity Source: RGD
  3. transferase activity Source: RGD

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. response to drug Source: RGD
  3. response to estrogen Source: RGD
  4. response to ethanol Source: RGD
  5. response to hormone Source: RGD
  6. response to hypoxia Source: RGD
  7. response to iron(II) ion Source: RGD
  8. response to lead ion Source: RGD
  9. response to nickel cation Source: RGD
  10. response to nutrient Source: RGD
  11. response to organic substance Source: RGD
  12. response to starvation Source: RGD
  13. response to sucrose Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactase-phlorizin hydrolase
Alternative name(s):
Lactase-glycosylceramidase
Including the following 2 domains:
Phlorizin hydrolase (EC:3.2.1.62)
Gene namesi
Name:Lct
Synonyms:Lph
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620823. Lct.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 18831862ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1884 – 190219HelicalSequence AnalysisAdd
BLAST
Topological domaini1903 – 192826CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. brush border Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Propeptidei20 – 867848Beta-glucosidasePRO_0000011771Add
BLAST
Chaini868 – 19281061Lactase-phlorizin hydrolasePRO_0000011772Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiQ02401.
PRIDEiQ02401.

PTM databases

PhosphoSiteiQ02401.

Expressioni

Tissue specificityi

Intestine.

Gene expression databases

GenevestigatoriQ02401.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ02401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati89 – 175871Add
BLAST
Repeati365 – 8494852Add
BLAST
Repeati886 – 13664813Add
BLAST
Repeati1371 – 18424724Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni89 – 184217544 X approximate repeatsAdd
BLAST

Domaini

The sequence exhibits 4 regions (I-IV) of internal homology; therefore LPH might have evolved by two cycles of partial gene duplication.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2723.
HOVERGENiHBG006290.
InParanoidiQ02401.
PhylomeDBiQ02401.

Family and domain databases

Gene3Di3.20.20.80. 4 hits.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 4 hits.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 4 hits.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02401-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MELPWTALFL STVLLGLSCQ GSDWESDRNF ISAAGPLTND LVLNLNYPPG
60 70 80 90 100
KQGSDVVSGN TDHLLCQQPL PSFLSQYFSS LRASQVTHYK VLLSWAQLLP
110 120 130 140 150
TGSSKNPDQE AVQCYRQLLQ SLKDAQLEPM VVLCHQTPPT SSAIQREGAF
160 170 180 190 200
ADLFADYATL AFQSFGDLVE IWFTFSDLEK VIMDLPHKDL KASALQTLSN
210 220 230 240 250
AHRRAFEIYH RKFSSQGGKL SVVLKAEDIP ELLPDPALAA LVQGSVDFLS
260 270 280 290 300
LDLSYECQSV ATLPQKLSEL QNLEPKVKVF IYTLKLEDCP ATGTSPSSLL
310 320 330 340 350
ISLLEAINKD QIQTVGFDVN AFLSCTSNSE ESPSCSLTDS LALQTEQQQE
360 370 380 390 400
TAVPSSPGSA YQRVWAAFAN QSREERDAFL QDVFPEGFLW GISTGAFNVE
410 420 430 440 450
GGWAEGGRGP SIWDHYGNLN AAEGQATAKV ASDSYHKPAS DVALLRGIRA
460 470 480 490 500
QVYKFSISWS GLFPLGQKST PNRQGVAYYN KLIDRLLDSH IEPMATLFHW
510 520 530 540 550
DLPQALQEQG GWQNESVVEA FLDYAAFCFS TFGDRVKLWV TFHEPWVMSY
560 570 580 590 600
AGYGTGQHAP AISDPGMASF KVAHLILKAH ARTWHLYDLH HRLQQQGRVG
610 620 630 640 650
IVLNSDLAEP LDRKSPQDLA AAERFLHFML GWFAHPIFVD GDYPTTSAQI
660 670 680 690 700
QHINQQCGHP LAQLPEFTEA EKRLLKGSAD FLGLSHYTSR LISKAGRQTC
710 720 730 740 750
TSSYDNIGGF SQHVDPEWPQ TASPWIRVVP WGIRRLLRFA SMEYTKGKLP
760 770 780 790 800
IFLAGNGMPV GEEADLFDDS VRVNYFNWYI NEVLKAVKED LVDVRSYIVR
810 820 830 840 850
SLIDGYEGPL GFSQRFGLYH VNFNDSSRPR TPRKSAYLFT SIIEKNGFSA
860 870 880 890 900
KKVKRNPLPV RADFTSRARV TDSLPSEVPS KAKISVEKFS KQPRFERDLF
910 920 930 940 950
YDGRFRDDFL WGVSSSPYQI EGGWNADGKG PSIWDNFTHT PGNGVKDNAT
960 970 980 990 1000
GDVACDSYHQ LDADLNILRT LKVKSYRFSI SWSRIFPTGR NSTINKQGVD
1010 1020 1030 1040 1050
YYNRLIDSLV DNNIFPMVTL FHWDLPQALQ DIGGWENPSL IELFDSYADY
1060 1070 1080 1090 1100
CFKTFGDRVK FWMTFNEPWC HVVLGYSSGI FPPSVQEPGW LPYKVSHIVI
1110 1120 1130 1140 1150
KAHARVYHTY DEKYRSEQKG VISLSLNTHW AEPKDPGLQR DVEAADRMLQ
1160 1170 1180 1190 1200
FTMGWFAHPI FKNGDYPDVM KWTVGNRSEL QHLASSRLPT FTEEEKNYVR
1210 1220 1230 1240 1250
GTADVFCHNT YTSVFVQHST PRLNPPSYDD DMELKLIEMN SSTGVMHQDV
1260 1270 1280 1290 1300
PWGTRRLLNW IKEEYGNIPI YITENGQGLE NPTLDDTERI FYHKTYINEA
1310 1320 1330 1340 1350
LKAYKLDGVD LRGYSAWTLM DDFEWLLGYT MRFGLYYVDF NHVSRPRTAR
1360 1370 1380 1390 1400
ASARYYPDLI ANNGMPLARE DEFLYGEFPK GFIWSAASAS YQVEGAWRAD
1410 1420 1430 1440 1450
GKGLSIWDTF SHTPLRIGND DNGDVACDSY HKIAEDVVAL QNLGVSHYRF
1460 1470 1480 1490 1500
SIAWSRILPD GTTKFINEAG LSYYVRFIDA LLAAGITPQV TIYHWDLPQA
1510 1520 1530 1540 1550
LQDVGGWENE TIVQRFKEYA DVLFQRLGDR VKFWITLNEP FVIAAQGYGT
1560 1570 1580 1590 1600
GVSAPGISFR PGTAPYIAGH NLIKAHAEAW HLYNDVYRAR QGGTISITIS
1610 1620 1630 1640 1650
SDWGEPRDPT NREHVEAARS YVQFMGGWFA HPIFKNGDYP EVMKTRIRDR
1660 1670 1680 1690 1700
SLGAGLNKSR LPEFTESEKS RIKGTFDFFG FNHNTTVLAY NLDYPAAFSS
1710 1720 1730 1740 1750
FDADRGVASI ADSSWPVSGS FWLKVTPFGF RRILNWLKEE YNNPPIYVTE
1760 1770 1780 1790 1800
NGVSRRGEPE LNDTDRIYYL RSYINEALKA VHDKVDLRGY TVWSIMDNFE
1810 1820 1830 1840 1850
WATGFAERFG VHFVNRSDPS LPRIPRASAK FYATIVRCNG FPDPAQGPHP
1860 1870 1880 1890 1900
CLQQPEDAAP TASPVQSEVP FLGLMLGIAE AQTALYVLFA LLLLGACSLA
1910 1920
FLTYNTGRRS KQGNAQPSQH QLSPISSF
Length:1,928
Mass (Da):217,268
Last modified:May 30, 2000 - v2
Checksum:i56DDCAAC4ACAEA85
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71A → R in AAA41539. (PubMed:1909681)Curated
Sequence conflicti113 – 1131Q → E in AAA41539. (PubMed:1909681)Curated
Sequence conflicti207 – 2071E → D in AAA41539. (PubMed:1909681)Curated
Sequence conflicti1337 – 13371Y → H(PubMed:1691182)Curated
Sequence conflicti1357 – 13593PDL → AEV(PubMed:1691182)Curated
Sequence conflicti1369 – 13702RE → GK(PubMed:1691182)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56748 Genomic DNA. Translation: CAA40070.1.
X56747 mRNA. Translation: CAA40069.1.
L04635 Genomic DNA. Translation: AAA41539.1.
PIRiJS0610.
UniGeneiRn.92376.

Genome annotation databases

UCSCiRGD:620823. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56748 Genomic DNA. Translation: CAA40070.1 .
X56747 mRNA. Translation: CAA40069.1 .
L04635 Genomic DNA. Translation: AAA41539.1 .
PIRi JS0610.
UniGenei Rn.92376.

3D structure databases

ProteinModelPortali Q02401.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q02401.
ChEMBLi CHEMBL3389.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

PTM databases

PhosphoSitei Q02401.

Proteomic databases

PaxDbi Q02401.
PRIDEi Q02401.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:620823. rat.

Organism-specific databases

RGDi 620823. Lct.

Phylogenomic databases

eggNOGi COG2723.
HOVERGENi HBG006290.
InParanoidi Q02401.
PhylomeDBi Q02401.

Miscellaneous databases

PROi Q02401.

Gene expression databases

Genevestigatori Q02401.

Family and domain databases

Gene3Di 3.20.20.80. 4 hits.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 4 hits.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 4 hits.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the precursor of intestinal lactase-phlorizin hydrolase from fetal rat."
    Duluc I., Boukamel R., Mantei N., Semenza G., Raul F., Freund J.-N.
    Gene 103:275-276(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Wistar.
    Tissue: Intestine.
  2. "The rat LPH gene 5' region: comparative structure with the human gene."
    Boukamel R., Freund J.-N.
    DNA Seq. 3:119-121(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192.
    Strain: Sprague-Dawley.
  3. "Coordinate expression of lactase-phlorizin hydrolase mRNA and enzyme levels in rat intestine during development."
    Bueller H.A., Kothe M.J., Goldman D.A., Grubman S.A., Sasak W.V., Matsudaira P.T., Montgomery R.K., Grand R.J.
    J. Biol. Chem. 265:6978-6983(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1288-1370.

Entry informationi

Entry nameiLPH_RAT
AccessioniPrimary (citable) accession number: Q02401
Secondary accession number(s): Q63712, Q63719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 30, 2000
Last modified: October 29, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3