Q02395 (MTF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metal-response element-binding transcription factor 2

Alternative name(s):
Metal regulatory transcription factor 2
Metal-response element DNA-binding protein M96
Polycomb-like protein 2
Short name=mPCl2
Zinc-regulated factor 1
Short name=ZiRF1

Gene names

Name:	Mtf2
Synonyms:	Pcl2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the metal-regulating-element (MRE) of metallothionein-1A gene promoter. Binding is zinc-dependent.
Subcellular location	Nucleus.
Sequence similarities	Contains 2 PHD-type zinc fingers.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Eed	Q921E6	3	EBI-2531578,EBI-904301
Ezh1	P70351	3	EBI-2531578,EBI-2531737
Ezh2	Q61188	4	EBI-2531578,EBI-904311

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q02395-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q02395-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 593

593

Metal-response element-binding transcription factor 2

PRO_0000059318

Regions

Zinc finger

102 – 157

56

PHD-type 1

Zinc finger

201 – 255

55

PHD-type 2

Compositional bias

338 – 341

4

Poly-Lys

Compositional bias

497 – 502

6

Poly-Arg

Amino acid modifications

Modified residue

24

1

Phosphothreonine By similarity

Modified residue

27

1

Phosphoserine By similarity

Modified residue

488

1

Phosphoserine By similarity

Natural variations

Alternative sequence

1 – 102

102

Missing in isoform 2.

VSP_016240

Experimental info

Sequence conflict

200 – 201

2

QQ → PE in AAC34714. Ref.3

Sequence conflict

282

1

Missing in AAC34714. Ref.3

Sequence conflict

394

1

E → K in AAH57163. Ref.2

Sequence conflict

397

1

G → K in AAH57163. Ref.2

Sequence conflict

519

1

D → G in BAC28027. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

593

Helix Strand Turn

Details...

Beta strand

105 – 108

4

Beta strand

119 – 121

3

Beta strand

123 – 125

3

Beta strand

128 – 130

3

Turn

131 – 133

3

Beta strand

134 – 136

3

Helix

140 – 144

5

Helix

152 – 156

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 22, 2005. Version 2. Checksum: C595438196DC3E0F	FASTA	593	66,924
	10 20 30 40 50 60 MRDSTGAGNS LVHKRSPLRR NQKTSASLNK LSLQDGHKAK KPACKFEEGQ DVLARWSDGL 70 80 90 100 110 120 FYLGTIKKIN ILKQSCFIIF EDSSKSWVLW KDIQTGATGS GEMVCTICQE EYSEAPNEMV 130 140 150 160 170 180 ICDKCGQGYH QLCHTPHIDS SVIDSDEKWL CRQCVFATTT KRGGALKKGP NAKALQVMKQ 190 200 210 220 230 240 TLPYSVADLE WDAGHKTNVQ QCYCYCGGPG DWYLKMLQCC KCKQWFHEAC VQCLQKPMLF 250 260 270 280 290 300 GDRFYTFICS VCSSGPEYLK RLPLQWVDIA HLCLYNLSVI HKKKYFDSEL ELMTYINENW 310 320 330 340 350 360 DRLHPGELAD TPKSERYEHV LEALNDYKTM FMSGKEIKKK KHLFGLRIRV PPVPPNVAFK 370 380 390 400 410 420 AEKEPEGTSH EFKIKGRKAS KPTSDSREVS NGIEKKGKKK SVGRPPGPYT RKMIQKTAEL 430 440 450 460 470 480 PLDKESVSEN PTLDLPCSIG RTEGIAHSSN TSDVDLTGAS SANETTSASI SRHCGLSDSR 490 500 510 520 530 540 KRTRTGRSWP AAIPHLRRRR GRLPRRALQT QNSEVVKDDE GKEDYQFEEL NTEILNNLAD 550 560 570 580 590 QELQLNHLKN SITSYFGAAG RIACGEKYRV LARRVTLDGK VQYLVEWEGA TAS
	Isoform 2 [UniParc]. Checksum: 7FBA64012D15553F	FASTA	491	55,586
	10 20 30 40 50 60 MVCTICQEEY SEAPNEMVIC DKCGQGYHQL CHTPHIDSSV IDSDEKWLCR QCVFATTTKR 70 80 90 100 110 120 GGALKKGPNA KALQVMKQTL PYSVADLEWD AGHKTNVQQC YCYCGGPGDW YLKMLQCCKC 130 140 150 160 170 180 KQWFHEACVQ CLQKPMLFGD RFYTFICSVC SSGPEYLKRL PLQWVDIAHL CLYNLSVIHK 190 200 210 220 230 240 KKYFDSELEL MTYINENWDR LHPGELADTP KSERYEHVLE ALNDYKTMFM SGKEIKKKKH 250 260 270 280 290 300 LFGLRIRVPP VPPNVAFKAE KEPEGTSHEF KIKGRKASKP TSDSREVSNG IEKKGKKKSV 310 320 330 340 350 360 GRPPGPYTRK MIQKTAELPL DKESVSENPT LDLPCSIGRT EGIAHSSNTS DVDLTGASSA 370 380 390 400 410 420 NETTSASISR HCGLSDSRKR TRTGRSWPAA IPHLRRRRGR LPRRALQTQN SEVVKDDEGK 430 440 450 460 470 480 EDYQFEELNT EILNNLADQE LQLNHLKNSI TSYFGAAGRI ACGEKYRVLA RRVTLDGKVQ 490 YLVEWEGATA S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Egg, Testis and Wolffian duct.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6 and NMRI. Tissue: Head, Mammary tumor and Placenta.
[3]	"Isolation of a cDNA encoding a metal response element binding protein using a novel expression cloning procedure: the one hybrid system." Inouye C., Remondelli P., Karin M., Elledge S. DNA Cell Biol. 13:731-742(1994) [PubMed: 7772254] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-476 (ISOFORM 2). Tissue: Lymphoma.
[4]	"Interactions of the zinc-regulated factor (ZiRF1) with the mouse metallothionein Ia promoter." Remondelli P., Leone A. Biochem. J. 323:79-85(1997) [PubMed: 9173905] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK032798 mRNA. Translation: BAC28027.1.
AK032819 mRNA. Translation: BAC28039.1.
AK031409 mRNA. Translation: BAC27390.1.
AK139685 mRNA. Translation: BAE24103.1.
BC057163 mRNA. Translation: AAH57163.1.
BC092237 mRNA. Translation: AAH92237.1.
BC100340 mRNA. Translation: AAI00341.1.
S78454 mRNA. Translation: AAC34714.1.

IPI

IPI00313570.
IPI00467892.

RefSeq

NP_038855.2. NM_013827.2.

UniGene

Mm.257149.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EQJ	NMR	-	A	40-98	[»]
2YT5	NMR	-	A	104-162	[»]

ProteinModelPortal

Q02395.

SMR

Q02395. Positions 36-162, 199-254.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q02395. 11 interactions.

STRING

Q02395.

PTM databases

PhosphoSite

Q02395.

2D gel databases

PMMA-2DPAGE

Q02395.

Proteomic databases

PRIDE

Q02395.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000081567; ENSMUSP00000080278; ENSMUSG00000029267.

GeneID

17765.

KEGG

mmu:17765.

UCSC

uc008ynj.1. mouse.
uc008ynk.1. mouse.

Organism-specific databases

CTD

22823.

MGI

MGI:105050. Mtf2.

Phylogenomic databases

eggNOG

roNOG05623.

HOGENOM

HBG713943.

HOVERGEN

HBG004755.

InParanoid

Q02395.

OrthoDB

EOG4TB4B3.

Gene expression databases

ArrayExpress

Q02395.

Bgee

Q02395.

Genevestigator

Q02395.

GermOnline

ENSMUSG00000029267. Mus musculus.

Family and domain databases

InterPro

IPR002999. Tudor.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 2 hits.

KO

K11485.

Pfam

PF00628. PHD. 1 hit.
[Graphical view]

SMART

SM00249. PHD. 2 hits.
SM00333. TUDOR. 1 hit.
[Graphical view]

SUPFAM

SSF57903. FYVE_PHD_ZnF. 2 hits.

PROSITE

PS01359. ZF_PHD_1. 2 hits.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

292469.

SOURCE

Search...

Entry information

Entry name

MTF2_MOUSE

Accession

Primary (citable) accession number: Q02395
Secondary accession number(s): Q569Z8 , Q6PG89, Q8BGP9, Q8BSJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 22, 2005
Last modified:	November 16, 2011
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program