Q02382 (RDRP_LRVLG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable RNA-directed RNA polymerase
EC=2.7.7.48

Gene names

Name:

ORF3

Organism

Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1) [Reference proteome]

Taxonomic identifier

58103 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Totiviridae › Leishmaniavirus

Virus host

Leishmania major [TaxID: 5664]

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which replicates the viral genome. Catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded from the virion into the cytoplasm where they function as mRNAs for translation of viral proteins and also as substrates for encapsidation to form new virions. Once encapsidated, the positive strand is converted to dsRNA by the RNA-directed RNA polymerase. Displays ssRNA-binding activity By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the totiviridae RNA-directed RNA polymerase family.

Ontologies

Keywords
Biological process	Viral RNA replication
Ligand	Nucleotide-binding RNA-binding
Molecular function	Hydrolase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	Probable RNA-directed RNA polymerase		PRO_0000404517

Sequences

	Sequence		Length	Mass (Da)
	Q02382 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A21CABDF4AA1A009	FASTA	874	98,321
	10 20 30 40 50 60 MQCPNQNHML VNRAMVVAAL DSFEDARRII SGVLDLSRLT NTSVKGQHTN DTNYFSRYSN 70 80 90 100 110 120 FFSQMPAIIL NQLKCCIKAQ VDDAVKMVLQ KAKKVEVSKP VEKMLTFTTL NTYLGYPEST 130 140 150 160 170 180 GCVMEYTEEQ SGPIAAKLLI TLLSSTLNAM VRPKSDPNIS QNKIPRHYYQ LKVHVGAQKK 190 200 210 220 230 240 VNLTAIEVIR GCQHECSVVY CEFVRYSAYF AGLYDDQVAA ILLYAVAAHN VQGFGARFCV 250 260 270 280 290 300 LWALMCVRIA GFADDINIYI KHRGMSGLLP QLVEMKCLLG RGVNEIDVET EARRRLDVGS 310 320 330 340 350 360 LSMQRLDENE LRAAVRLIYS EELRRPVTYP LICDFWSSRW LWAANGSHSR ALEHAHPELA 370 380 390 400 410 420 TRKEGQAYRK AVMEQWQHNP MDRWDGTVYV TPSAKLEHGK TRLLLACDTL SYMWFEYALR 430 440 450 460 470 480 PVERIWENSN VILDPGSMGN CGIATRINGW RNGMPGQSFF AVDYDDFNSQ HTLMSQKIVF 490 500 510 520 530 540 EELFHHIGYN ASWVKTLVDS FDSMELWIKG KCAGIMAGTL MSGHRATSFI NSVLNRAYII 550 560 570 580 590 600 CAGGHVPTSM HVGDDILMSC TLGHADNLIA NLNRKGVRLN ASKQVFSKTS GEFLRVAHRE 610 620 630 640 650 660 HTSHGYLARV ISSAVSGNWV SDHTLNQQEA LMNAIVCCRG ILNRSLPGEK NPVVRVISRS 670 680 690 700 710 720 VSKRTKIEEK TIRLLLSGRA CLKGGVVYGE QTNYIQVYRI NCRVERSEEK LPPYRHATED 730 740 750 760 770 780 YLNNHLADIE VMAVRQYGSD IADIMAQASW KKSMSTEGAE DVSRLSLQRD KTLPCLHCIT 790 800 810 820 830 840 EKETSLLPVR YGLFSSYPIL MMLKDRIPIK EALKLAVTIG YRPQPNSDLE LDLWGESNNS 850 860 870 CAIEGVLPYN EATSLAQKLP CGGVVIQVIH NVYV

References

[1]	"Molecular organization of Leishmania RNA virus 1." Stuart K.D., Weeks R., Guilbride L., Myler P.J. Proc. Natl. Acad. Sci. U.S.A. 89:8596-8600(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M92355 Genomic RNA. Translation: AAB50024.1.
PIR	C46171.
RefSeq	NP_041191.1. NC_002063.1.
3D structure databases
ProteinModelPortal	Q02382.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1446405.
Family and domain databases
InterPro	IPR001795. RNA-dir_pol_luteovirus. [Graphical view]
Pfam	PF02123. RdRP_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_LRVLG

Accession

Primary (citable) accession number: Q02382

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program