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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Gene

NDUFS5

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
Alternative name(s):
Complex I-15 kDa
Short name:
CI-15 kDa
NADH-ubiquinone oxidoreductase 15 kDa subunit
Gene namesi
Name:NDUFS5
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 106105NADH dehydrogenase [ubiquinone] iron-sulfur protein 5PRO_0000118785Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 66Sequence analysis
Disulfide bondi43 ↔ 56Sequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ02379.

Interactioni

Subunit structurei

Mammalian complex I is composed of 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.2 Publications

Protein-protein interaction databases

IntActiQ02379. 2 interactions.
STRINGi9913.ENSBTAP00000013507.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi33 – 4311C-X9-C motif 1Add
BLAST
Motifi56 – 6611C-X9-C motif 2Add
BLAST

Domaini

Contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.By similarity

Sequence similaritiesi

Belongs to the complex I NDUFS5 subunit family.Curated

Phylogenomic databases

eggNOGiKOG4110. Eukaryota.
ENOG41123TD. LUCA.
GeneTreeiENSGT00390000002919.
HOGENOMiHOG000264236.
HOVERGENiHBG001646.
InParanoidiQ02379.
KOiK03938.
OMAiFEDFYEC.
OrthoDBiEOG7C8GKB.
TreeFamiTF332111.

Family and domain databases

InterProiIPR019342. NADH_UbQ_OxRdtase_FeS-su5.
[Graphical view]
PfamiPF10200. Ndufs5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02379-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFFDVQKRL GVDLDRWMTI QSAEQPHKIP SRCHAFEKEW IECAHGIGSI
60 70 80 90 100
RAEKECKIEF EDFRECLLRQ KTMKRLHAIR RQREKLIKEG KYTPPPHHSG

QEEPRS
Length:106
Mass (Da):12,668
Last modified:January 23, 2007 - v2
Checksum:i75BE02EF2C1A9144
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63220 mRNA. Translation: CAA44905.1.
BC102919 mRNA. Translation: AAI02920.1.
PIRiS28239.
RefSeqiNP_788825.1. NM_176652.2.
UniGeneiBt.57.

Genome annotation databases

EnsembliENSBTAT00000013507; ENSBTAP00000013507; ENSBTAG00000010232.
GeneIDi338057.
KEGGibta:338057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63220 mRNA. Translation: CAA44905.1.
BC102919 mRNA. Translation: AAI02920.1.
PIRiS28239.
RefSeqiNP_788825.1. NM_176652.2.
UniGeneiBt.57.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ02379. 2 interactions.
STRINGi9913.ENSBTAP00000013507.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiQ02379.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000013507; ENSBTAP00000013507; ENSBTAG00000010232.
GeneIDi338057.
KEGGibta:338057.

Organism-specific databases

CTDi4725.

Phylogenomic databases

eggNOGiKOG4110. Eukaryota.
ENOG41123TD. LUCA.
GeneTreeiENSGT00390000002919.
HOGENOMiHOG000264236.
HOVERGENiHBG001646.
InParanoidiQ02379.
KOiK03938.
OMAiFEDFYEC.
OrthoDBiEOG7C8GKB.
TreeFamiTF332111.

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Miscellaneous databases

NextBioi20812508.

Family and domain databases

InterProiIPR019342. NADH_UbQ_OxRdtase_FeS-su5.
[Graphical view]
PfamiPF10200. Ndufs5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction."
    Walker J.E., Arizmendi J.M., Dupuis A., Fearnley I.M., Finel M., Medd S.M., Pilkington S.J., Runswick M.J., Skehel J.M.
    J. Mol. Biol. 226:1051-1072(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-23.
    Tissue: Heart.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thymus.
  3. "Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme."
    Sazanov L.A., Peak-Chew S.Y., Fearnley I.M., Walker J.E.
    Biochemistry 39:7229-7235(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, SUBUNIT, IDENTIFICATION IN COMPLEX I.
  4. "Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry."
    Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A., Robinson N.C.
    Anal. Biochem. 382:116-121(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN COMPLEX I.

Entry informationi

Entry nameiNDUS5_BOVIN
AccessioniPrimary (citable) accession number: Q02379
Secondary accession number(s): Q3ZC47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: February 17, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.