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Protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Gene

NDUFA2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
Alternative name(s):
Complex I-B8
Short name:
CI-B8
NADH-ubiquinone oxidoreductase B8 subunit
Gene namesi
Name:NDUFA2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

  • mitochondrial membrane Source: AgBase
  • mitochondrial respiratory chain complex I Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 9998NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2PRO_0000118788Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Disulfide bondi24 ↔ 58Redox-activeBy similarity
Modified residuei64 – 641N6-acetyllysine; alternateBy similarity
Modified residuei64 – 641N6-succinyllysine; alternateBy similarity
Modified residuei75 – 751N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiQ02370.
PRIDEiQ02370.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.2 Publications

Protein-protein interaction databases

IntActiQ02370. 18 interactions.
STRINGi9913.ENSBTAP00000020018.

Structurei

3D structure databases

ProteinModelPortaliQ02370.
SMRiQ02370. Positions 15-99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiKOG3446. Eukaryota.
ENOG41124JV. LUCA.
GeneTreeiENSGT00390000006178.
HOGENOMiHOG000201916.
HOVERGENiHBG082012.
InParanoidiQ02370.
KOiK03946.
OMAiCKASKGA.
OrthoDBiEOG779P1D.
TreeFamiTF300229.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR016464. NADH_Ub_cplx-1_asu_su-2.
IPR007741. Ribosome/NADH_DH.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR12878. PTHR12878. 1 hit.
PfamiPF05047. L51_S25_CI-B8. 1 hit.
[Graphical view]
PIRSFiPIRSF005822. NDUA2. 1 hit.
SMARTiSM00916. L51_S25_CI-B8. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAAIRGV RGKLGLREIR IHLCQRSPGS QGVRDFIEKR YVELKKANPD
60 70 80 90
LPILIRECSD VQPKLWARYA FGQEKNVSLN NFSADQVTRA LENVLSSKA
Length:99
Mass (Da):11,080
Last modified:January 23, 2007 - v2
Checksum:iB844420E64652EC2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441L → V in ABC02172 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63219 mRNA. Translation: CAA44904.1.
AY227782 Genomic DNA. Translation: ABC02172.1.
BC118428 mRNA. Translation: AAI18429.1.
PIRiS28249.
RefSeqiNP_787009.1. NM_175815.2.
UniGeneiBt.65.

Genome annotation databases

EnsembliENSBTAT00000020018; ENSBTAP00000020018; ENSBTAG00000015041.
GeneIDi327698.
KEGGibta:327698.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63219 mRNA. Translation: CAA44904.1.
AY227782 Genomic DNA. Translation: ABC02172.1.
BC118428 mRNA. Translation: AAI18429.1.
PIRiS28249.
RefSeqiNP_787009.1. NM_175815.2.
UniGeneiBt.65.

3D structure databases

ProteinModelPortaliQ02370.
SMRiQ02370. Positions 15-99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ02370. 18 interactions.
STRINGi9913.ENSBTAP00000020018.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiQ02370.
PRIDEiQ02370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000020018; ENSBTAP00000020018; ENSBTAG00000015041.
GeneIDi327698.
KEGGibta:327698.

Organism-specific databases

CTDi4695.

Phylogenomic databases

eggNOGiKOG3446. Eukaryota.
ENOG41124JV. LUCA.
GeneTreeiENSGT00390000006178.
HOGENOMiHOG000201916.
HOVERGENiHBG082012.
InParanoidiQ02370.
KOiK03946.
OMAiCKASKGA.
OrthoDBiEOG779P1D.
TreeFamiTF300229.

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR016464. NADH_Ub_cplx-1_asu_su-2.
IPR007741. Ribosome/NADH_DH.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR12878. PTHR12878. 1 hit.
PfamiPF05047. L51_S25_CI-B8. 1 hit.
[Graphical view]
PIRSFiPIRSF005822. NDUA2. 1 hit.
SMARTiSM00916. L51_S25_CI-B8. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction."
    Walker J.E., Arizmendi J.M., Dupuis A., Fearnley I.M., Finel M., Medd S.M., Pilkington S.J., Runswick M.J., Skehel J.M.
    J. Mol. Biol. 226:1051-1072(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 40-45; 47-61 AND 63-68.
    Tissue: Heart.
  2. "Genomic sequencing of the T cell receptor alpha locus."
    Conrad M.L., Mawer M.A., Davis S.K., Koop B.F.
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal cerebellum.
  4. "Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme."
    Sazanov L.A., Peak-Chew S.Y., Fearnley I.M., Walker J.E.
    Biochemistry 39:7229-7235(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, SUBUNIT, IDENTIFICATION IN COMPLEX I.
  5. "Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry."
    Lemma-Gray P., Valusova E., Carroll C.A., Weintraub S.T., Musatov A., Robinson N.C.
    Anal. Biochem. 382:116-121(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN COMPLEX I.

Entry informationi

Entry nameiNDUA2_BOVIN
AccessioniPrimary (citable) accession number: Q02370
Secondary accession number(s): Q148D8, Q2VYC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.