Q02363 (ID2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-binding protein inhibitor ID-2

Alternative name(s):
Class B basic helix-loop-helix protein 26
Short name=bHLHb26
Inhibitor of DNA binding 2

Gene names

Name:	ID2
Synonyms:	BHLHB26

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
Subunit structure	Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5 By similarity. Interacts with NR0B2. Ref.4
Subcellular location	Cytoplasm By similarity. Nucleus.
Tissue specificity	Highly expressed in early fetal tissues, including those of the central nervous system.
Developmental stage	Found in most early fetal tissues but not in the corresponding mature tissues.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Developmental protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Peyer's patch development Inferred from electronic annotation. Source: Compara adipose tissue development Inferred from electronic annotation. Source: Compara bundle of His development Inferred from electronic annotation. Source: Compara cell maturation Inferred from electronic annotation. Source: Compara cellular response to lithium ion Inferred from electronic annotation. Source: Compara cellular senescence Inferred from sequence or structural similarity. Source: UniProtKB embryonic digestive tract morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB endodermal digestive tract morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB entrainment of circadian clock Inferred from electronic annotation. Source: Compara enucleate erythrocyte differentiation Inferred from electronic annotation. Source: Compara epithelial cell differentiation involved in mammary gland alveolus development Inferred from sequence or structural similarity. Source: UniProtKB mammary gland epithelial cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB metanephros development Inferred from electronic annotation. Source: Compara natural killer cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of B cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of DNA binding Inferred from electronic annotation. Source: Compara negative regulation of neural precursor cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of oligodendrocyte differentiation Inferred from electronic annotation. Source: Compara negative regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara negative regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 14627819. Source: GDB negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from direct assay PubMed 14627819. Source: GDB neuron fate commitment Inferred from sequence or structural similarity. Source: UniProtKB olfactory bulb development Inferred from electronic annotation. Source: Compara oligodendrocyte development Inferred from electronic annotation. Source: Compara positive regulation of astrocyte differentiation Inferred from electronic annotation. Source: Compara positive regulation of blood pressure Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell cycle Inferred from electronic annotation. Source: Compara positive regulation of cell cycle arrest Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of erythrocyte differentiation Inferred from electronic annotation. Source: Compara positive regulation of fat cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of macrophage differentiation Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription involved in G1/S phase of mitotic cell cycle Inferred by curator PubMed 16311606. Source: BHF-UCL regulation of G1/S transition of mitotic cell cycle Inferred from mutant phenotype PubMed 16311606. Source: BHF-UCL regulation of lipid metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	chromatin Inferred from electronic annotation. Source: Compara cytosol Inferred from direct assay PubMed 16311606. Source: BHF-UCL nucleus Inferred from direct assay PubMed 16311606. Source: BHF-UCL protein complex Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TCF3	P15923-1	3	EBI-713450,EBI-769645

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 134

134

DNA-binding protein inhibitor ID-2

PRO_0000127240

Regions

Domain

23 – 75

53

bHLH

Motif

106 – 115

10

Nuclear export signal By similarity

Experimental info

Sequence conflict

68

1

V → L in AAA58681. Ref.2

Sequence conflict

98

1

A → R in AAA58681. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

134

Helix Strand Turn

Details...

Helix

32 – 49

18

Beta strand

55 – 57

3

Helix

61 – 80

20

Sequences

	Sequence		Length	Mass (Da)
	Q02363 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A0D98B96396EB11E	FASTA	134	14,917
	10 20 30 40 50 60 MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV PSIPQNKKVS 70 80 90 100 110 120 KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR TPLTTLNTDI SILSLQASEF 130 PSELMSNDSK ALCG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Id-related genes encoding helix-loop-helix proteins are required for G1 progression and are repressed in senescent human fibroblasts." Hara E., Yamaguchi T., Nojima H., Ide T., Campisi J., Okayama H., Oda K. J. Biol. Chem. 269:2139-2145(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"A human Id-like helix-loop-helix protein expressed during early development." Biggs J., Murphy E.V., Israel M.A. Proc. Natl. Acad. Sci. U.S.A. 89:1512-1516(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[4]	"Orphan nuclear receptor small heterodimer partner, a novel corepressor for a basic helix-loop-helix transcription factor BETA2/neuroD." Kim J.Y., Chu K., Kim H.J., Seong H.A., Park K.C., Sanyal S., Takeda J., Ha H., Shong M., Tsai M.J., Choi H.S. Mol. Endocrinol. 18:776-790(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NR0B2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D13891 mRNA. Translation: BAA02990.1.
M97796 mRNA. Translation: AAA58681.1.
BC030639 mRNA. Translation: AAH30639.1.

IPI

IPI00294210.

PIR

A40227.
JC2007.

RefSeq

NP_002157.2. NM_002166.4.

UniGene

Hs.180919.
Hs.726053.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4AYA	X-ray	2.10	A/B	1-82	[»]

ProteinModelPortal

Q02363.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46874N.

IntAct

Q02363. 9 interactions.

MINT

MINT-1372728.

STRING

9606.ENSP00000234091.

PTM databases

PhosphoSite

Q02363.

Polymorphism databases

DMDM

729806.

Proteomic databases

PaxDb

Q02363.

PRIDE

Q02363.

Protocols and materials databases

DNASU

3398.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000234091; ENSP00000234091; ENSG00000115738.
ENST00000331129; ENSP00000385465; ENSG00000115738.
ENST00000396290; ENSP00000379585; ENSG00000115738.

GeneID

3398.

KEGG

hsa:3398.

UCSC

uc002qza.3. human.

Organism-specific databases

CTD

3398.

GeneCards

GC02P008818.

H-InvDB

HIX0204445.

HGNC

HGNC:5361. ID2.

HPA

HPA027612.
HPA028681.

MIM

600386. gene.

neXtProt

NX_Q02363.

PharmGKB

PA29609.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG76382.

HOGENOM

HOG000234788.

HOVERGEN

HBG009009.

InParanoid

Q02363.

KO

K04680.

OMA

NVSKMEI.

OrthoDB

EOG4MPHRM.

PhylomeDB

Q02363.

Enzyme and pathway databases

Pathway_Interaction_DB

hif1_tfpathway. HIF-1-alpha transcription factor network.

Gene expression databases

ArrayExpress

Q02363.

Bgee

Q02363.

CleanEx

HS_ID2.

Genevestigator

Q02363.

GermOnline

ENSG00000115738. Homo sapiens.

Family and domain databases

Gene3D

4.10.280.10. 1 hit.

InterPro

IPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]

PANTHER

PTHR11723. PTHR11723. 1 hit.

Pfam

PF00010. HLH. 1 hit.
[Graphical view]

SMART

SM00353. HLH. 1 hit.
[Graphical view]

SUPFAM

SSF47459. HLH_basic. 1 hit.

PROSITE

PS50888. BHLH. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

3398.

NextBio

13428.

SOURCE

Search...

Entry information

Entry name

ID2_HUMAN

Accession

Primary (citable) accession number: Q02363

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.