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Q02363 (ID2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-binding protein inhibitor ID-2
Alternative name(s):
Class B basic helix-loop-helix protein 26
Short name=bHLHb26
Inhibitor of DNA binding 2
Gene names
Name:ID2
Synonyms:BHLHB26
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length134 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.

Subunit structure

Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5 By similarity. Interacts with NR0B2. Ref.4

Subcellular location

Cytoplasm By similarity. Nucleus.

Tissue specificity

Highly expressed in early fetal tissues, including those of the central nervous system.

Developmental stage

Found in most early fetal tissues but not in the corresponding mature tissues.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Molecular functionDevelopmental protein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processPeyer's patch development

Inferred from electronic annotation. Source: Ensembl

adipose tissue development

Inferred from electronic annotation. Source: Ensembl

bundle of His development

Inferred from electronic annotation. Source: Ensembl

cell maturation

Inferred from electronic annotation. Source: Ensembl

cellular response to lithium ion

Inferred from electronic annotation. Source: Ensembl

cellular senescence

Inferred from sequence or structural similarity. Source: UniProtKB

embryonic digestive tract morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

endodermal digestive tract morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

entrainment of circadian clock

Inferred from electronic annotation. Source: Ensembl

enucleate erythrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

epithelial cell differentiation involved in mammary gland alveolus development

Inferred from sequence or structural similarity. Source: UniProtKB

mammary gland alveolus development

Inferred from sequence or structural similarity. Source: UniProtKB

mammary gland epithelial cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

membranous septum morphogenesis

Inferred from electronic annotation. Source: Ensembl

metanephros development

Inferred from electronic annotation. Source: Ensembl

multicellular organismal development

Traceable author statement Ref.2. Source: ProtInc

natural killer cell differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of B cell differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of DNA binding

Inferred from electronic annotation. Source: Ensembl

negative regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of neural precursor cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of neuron differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of oligodendrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 14627819. Source: GDB

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 14627819. Source: GDB

neuron fate commitment

Inferred from sequence or structural similarity. Source: UniProtKB

olfactory bulb development

Inferred from electronic annotation. Source: Ensembl

oligodendrocyte development

Inferred from electronic annotation. Source: Ensembl

positive regulation of astrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of blood pressure

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cell cycle

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell cycle arrest

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of erythrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of fat cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of macrophage differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of smooth muscle cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription involved in G1/S transition of mitotic cell cycle

Inferred by curator PubMed 16311606. Source: BHF-UCL

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of G1/S transition of mitotic cell cycle

Inferred from mutant phenotype PubMed 16311606. Source: BHF-UCL

regulation of lipid metabolic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcentrosome

Inferred from direct assay. Source: HPA

chromatin

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from direct assay. Source: HPA

cytosol

Inferred from direct assay PubMed 16311606. Source: BHF-UCL

nucleus

Inferred from direct assay PubMed 16311606. Source: BHF-UCL

protein complex

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionion channel binding

Inferred from physical interaction PubMed 16311606. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CCNDBP1O952734EBI-713450,EBI-748961
TCF3P15923-13EBI-713450,EBI-769645

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 134134DNA-binding protein inhibitor ID-2
PRO_0000127240

Regions

Domain23 – 7553bHLH
Motif106 – 11510Nuclear export signal By similarity

Experimental info

Sequence conflict681V → L in AAA58681. Ref.2
Sequence conflict981A → R in AAA58681. Ref.2

Secondary structure

....... 134
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q02363 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: A0D98B96396EB11E

FASTA13414,917
        10         20         30         40         50         60 
MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV PSIPQNKKVS 

        70         80         90        100        110        120 
KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR TPLTTLNTDI SILSLQASEF 

       130 
PSELMSNDSK ALCG 

« Hide

References

« Hide 'large scale' references
[1]"Id-related genes encoding helix-loop-helix proteins are required for G1 progression and are repressed in senescent human fibroblasts."
Hara E., Yamaguchi T., Nojima H., Ide T., Campisi J., Okayama H., Oda K.
J. Biol. Chem. 269:2139-2145(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"A human Id-like helix-loop-helix protein expressed during early development."
Biggs J., Murphy E.V., Israel M.A.
Proc. Natl. Acad. Sci. U.S.A. 89:1512-1516(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Hippocampus.
[4]"Orphan nuclear receptor small heterodimer partner, a novel corepressor for a basic helix-loop-helix transcription factor BETA2/neuroD."
Kim J.Y., Chu K., Kim H.J., Seong H.A., Park K.C., Sanyal S., Takeda J., Ha H., Shong M., Tsai M.J., Choi H.S.
Mol. Endocrinol. 18:776-790(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NR0B2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13891 mRNA. Translation: BAA02990.1.
M97796 mRNA. Translation: AAA58681.1.
BC030639 mRNA. Translation: AAH30639.1.
PIRA40227.
JC2007.
RefSeqNP_002157.2. NM_002166.4.
UniGeneHs.180919.
Hs.726053.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4AYAX-ray2.10A/B1-82[»]
ProteinModelPortalQ02363.
SMRQ02363. Positions 30-82.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109624. 34 interactions.
DIPDIP-46874N.
IntActQ02363. 14 interactions.
MINTMINT-1372728.
STRING9606.ENSP00000234091.

PTM databases

PhosphoSiteQ02363.

Polymorphism databases

DMDM729806.

Proteomic databases

PaxDbQ02363.
PRIDEQ02363.

Protocols and materials databases

DNASU3398.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000234091; ENSP00000234091; ENSG00000115738.
ENST00000331129; ENSP00000385465; ENSG00000115738.
ENST00000396290; ENSP00000379585; ENSG00000115738.
GeneID3398.
KEGGhsa:3398.
UCSCuc002qza.3. human.

Organism-specific databases

CTD3398.
GeneCardsGC02P008818.
H-InvDBHIX0204445.
HGNCHGNC:5361. ID2.
HPAHPA027612.
HPA028681.
MIM600386. gene.
neXtProtNX_Q02363.
PharmGKBPA29609.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG76382.
HOGENOMHOG000234788.
HOVERGENHBG009009.
InParanoidQ02363.
KOK17693.
OMANVSKMEI.
OrthoDBEOG7X9G8G.
PhylomeDBQ02363.
TreeFamTF326217.

Gene expression databases

ArrayExpressQ02363.
BgeeQ02363.
CleanExHS_ID2.
GenevestigatorQ02363.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERPTHR11723. PTHR11723. 1 hit.
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiID2.
GenomeRNAi3398.
NextBio13428.
PROQ02363.
SOURCESearch...

Entry information

Entry nameID2_HUMAN
AccessionPrimary (citable) accession number: Q02363
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM