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Protein

DNA-binding protein inhibitor ID-2

Gene

ID2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processBiological rhythms, Transcription, Transcription regulation

Enzyme and pathway databases

SIGNORiQ02363

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein inhibitor ID-2
Alternative name(s):
Class B basic helix-loop-helix protein 26
Short name:
bHLHb26
Inhibitor of DNA binding 2
Inhibitor of differentiation 2
Gene namesi
Name:ID2
Synonyms:BHLHB26
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115738.9
HGNCiHGNC:5361 ID2
MIMi600386 gene
neXtProtiNX_Q02363

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3398
OpenTargetsiENSG00000115738
PharmGKBiPA29609

Polymorphism and mutation databases

BioMutaiID2
DMDMi729806

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272401 – 134DNA-binding protein inhibitor ID-2Add BLAST134

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineCombined sources1
Modified residuei25PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ02363
PaxDbiQ02363
PeptideAtlasiQ02363
PRIDEiQ02363

PTM databases

iPTMnetiQ02363
PhosphoSitePlusiQ02363

Expressioni

Tissue specificityi

Highly expressed in early fetal tissues, including those of the central nervous system.

Developmental stagei

Found in most early fetal tissues but not in the corresponding mature tissues.

Gene expression databases

BgeeiENSG00000115738
CleanExiHS_ID2
ExpressionAtlasiQ02363 baseline and differential
GenevisibleiQ02363 HS

Organism-specific databases

HPAiHPA027612

Interactioni

Subunit structurei

Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5 (By similarity). Interacts with NR0B2. Interacts with CLOCK and ARNTL/BMAL1.By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ion channel binding Source: BHF-UCL
  • protein dimerization activity Source: InterPro

Protein-protein interaction databases

BioGridi109624, 75 interactors
DIPiDIP-46874N
ELMiQ02363
IntActiQ02363, 29 interactors
MINTiQ02363
STRINGi9606.ENSP00000234091

Structurei

Secondary structure

1134
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 49Combined sources18
Beta strandi55 – 57Combined sources3
Helixi61 – 80Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AYAX-ray2.10A/B1-82[»]
ProteinModelPortaliQ02363
SMRiQ02363
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 75bHLHPROSITE-ProRule annotationAdd BLAST53

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 115Nuclear export signalBy similarity10

Domaini

The bHLH domain is essential for its repressor activity towards the CLOCK-ARNTL/BMAL1 heterodimer.By similarity

Phylogenomic databases

eggNOGiENOG410IVXR Eukaryota
ENOG4111PGK LUCA
GeneTreeiENSGT00390000000908
HOGENOMiHOG000234788
HOVERGENiHBG009009
InParanoidiQ02363
KOiK17693
OMAiFPSEFMS
OrthoDBiEOG091G0VHR
PhylomeDBiQ02363
TreeFamiTF326217

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR026052 DNA-bd_prot-inh
IPR036638 HLH_DNA-bd_sf
PANTHERiPTHR11723 PTHR11723, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

Q02363-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV
60 70 80 90 100
PSIPQNKKVS KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR
110 120 130
TPLTTLNTDI SILSLQASEF PSELMSNDSK ALCG
Length:134
Mass (Da):14,917
Last modified:February 1, 1995 - v1
Checksum:iA0D98B96396EB11E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68V → L in AAA58681 (PubMed:1741406).Curated1
Sequence conflicti98A → R in AAA58681 (PubMed:1741406).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13891 mRNA Translation: BAA02990.1
M97796 mRNA Translation: AAA58681.1
BC030639 mRNA Translation: AAH30639.1
CCDSiCCDS1659.1
PIRiA40227
JC2007
RefSeqiNP_002157.2, NM_002166.4
UniGeneiHs.180919
Hs.726053

Genome annotation databases

EnsembliENST00000234091; ENSP00000234091; ENSG00000115738
ENST00000331129; ENSP00000385465; ENSG00000115738
ENST00000396290; ENSP00000379585; ENSG00000115738
GeneIDi3398
KEGGihsa:3398

Similar proteinsi

Entry informationi

Entry nameiID2_HUMAN
AccessioniPrimary (citable) accession number: Q02363
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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