Q02363 (ID2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-binding protein inhibitor ID-2

Alternative name(s):
Class B basic helix-loop-helix protein 26
Short name=bHLHb26
Inhibitor of DNA binding 2

Gene names

Name:	ID2
Synonyms:	BHLHB26

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
Subunit structure	Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5 By similarity. Interacts with NR0B2. Ref.4
Subcellular location	Cytoplasm By similarity. Nucleus.
Tissue specificity	Highly expressed in early fetal tissues, including those of the central nervous system.
Developmental stage	Found in most early fetal tissues but not in the corresponding mature tissues.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular senescence Inferred from sequence or structural similarity. Source: UniProtKB embryonic digestive tract morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB endodermal digestive tract morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB epithelial cell differentiation involved in mammary gland alveolus development Inferred from sequence or structural similarity. Source: UniProtKB mammary gland epithelial cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of neural precursor cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: GDB neuron fate commitment Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of blood pressure Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell cycle arrest Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of smooth muscle cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred by curator. Source: GDB protein complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.4. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TCF3	P15923-1	3	EBI-713450,EBI-769645

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	DNA-binding protein inhibitor ID-2		PRO_0000127240
Regions
	Domain	36 – 76	41	Helix-loop-helix motif
	Motif	106 – 115	10	Nuclear export signal By similarity
Experimental info
	Sequence conflict	68	1	V → L in AAA58681. Ref.2
	Sequence conflict	98	1	A → R in AAA58681. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q02363 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A0D98B96396EB11E	FASTA	134	14,917
	10 20 30 40 50 60 MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV PSIPQNKKVS 70 80 90 100 110 120 KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR TPLTTLNTDI SILSLQASEF 130 PSELMSNDSK ALCG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Id-related genes encoding helix-loop-helix proteins are required for G1 progression and are repressed in senescent human fibroblasts." Hara E., Yamaguchi T., Nojima H., Ide T., Campisi J., Okayama H., Oda K. J. Biol. Chem. 269:2139-2145(1994) [PubMed: 8294468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"A human Id-like helix-loop-helix protein expressed during early development." Biggs J., Murphy E.V., Israel M.A. Proc. Natl. Acad. Sci. U.S.A. 89:1512-1516(1992) [PubMed: 1741406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[4]	"Orphan nuclear receptor small heterodimer partner, a novel corepressor for a basic helix-loop-helix transcription factor BETA2/neuroD." Kim J.Y., Chu K., Kim H.J., Seong H.A., Park K.C., Sanyal S., Takeda J., Ha H., Shong M., Tsai M.J., Choi H.S. Mol. Endocrinol. 18:776-790(2004) [PubMed: 14752053] [Abstract] Cited for: INTERACTION WITH NR0B2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13891 mRNA. Translation: BAA02990.1. M97796 mRNA. Translation: AAA58681.1. BC030639 mRNA. Translation: AAH30639.1.
IPI	IPI00294210.
PIR	A40227. JC2007.
RefSeq	NP_002157.2. NM_002166.4.
UniGene	Hs.180919.
3D structure databases
ProteinModelPortal	Q02363.
SMR	Q02363. Positions 30-77.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46874N.
IntAct	Q02363. 10 interactions.
MINT	MINT-1372728.
STRING	Q02363.
PTM databases
PhosphoSite	Q02363.
Polymorphism databases
DMDM	729806.
Proteomic databases
PRIDE	Q02363.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000234091; ENSP00000234091; ENSG00000115738. ENST00000331129; ENSP00000385465; ENSG00000115738. ENST00000396290; ENSP00000379585; ENSG00000115738.
GeneID	3398.
KEGG	hsa:3398.
UCSC	uc002qza.1. human.
Organism-specific databases
CTD	3398.
GeneCards	GC02P008772.
H-InvDB	HIX0001802.
HGNC	HGNC:5361. ID2.
HPA	HPA027612. HPA028681.
MIM	600386. gene.
neXtProt	NX_Q02363.
PharmGKB	PA29609.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17262.
HOGENOM	HBG713167.
HOVERGEN	HBG009009.
InParanoid	Q02363.
OMA	QRPGQSQ.
OrthoDB	EOG4MPHRM.
PhylomeDB	Q02363.
Enzyme and pathway databases
Pathway_Interaction_DB	hif1_tfpathway. HIF-1-alpha transcription factor network.
Gene expression databases
ArrayExpress	Q02363.
Bgee	Q02363.
CleanEx	HS_ID2.
Genevestigator	Q02363.
GermOnline	ENSG00000115738. Homo sapiens.
Family and domain databases
InterPro	IPR011598. HLH_DNA-bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
KO	K04680.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	13428.
SOURCE	Search...

Entry information

Entry name

ID2_HUMAN

Accession

Primary (citable) accession number: Q02363

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.