Reviewed, UniProtKB/Swiss-Prot Q02356 (AMPD2_RAT)

Names and origin

Protein names

Recommended name:
    AMP deaminase 2
    EC=3.5.4.6
Alternative name(s):
    AMP deaminase isoform L

Gene names

Name:

Ampd2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	AMP deaminase plays a critical role in energy metabolism.
Catalytic activity	AMP + H2O = IMP + NH3.
Pathway	Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.
Subunit structure	Homotetramer.
Tissue specificity	Three isoforms are present in mammals: AMP deaminase 1 is the predominant form in skeletal muscle; AMP deaminase 2 predominates in smooth muscle, non-muscle tissue, embryonic muscle and undifferentiated myoblasts; AMP deaminase 3 is found in erythrocytes.
Sequence similarities	Belongs to the adenosine and AMP deaminases family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	purine base metabolic process Inferred from electronic annotation. Source: InterPro purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP deaminase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 824	824	AMP deaminase 2		PRO_0000194409
Sites
	Active site	424	1	Potential
	Active site	634	1	Potential
	Active site	710	1	Potential
	Active site	711	1	Potential
Amino acid modifications
	Modified residue	45	1	Phosphoserine By similarity
	Modified residue	90	1	Phosphotyrosine By similarity
	Modified residue	113	1	Phosphoserine By similarity
	Modified residue	135	1	Phosphoserine By similarity
	Modified residue	137	1	Phosphoserine By similarity
	Modified residue	440	1	Phosphotyrosine By similarity
	Modified residue	446	1	Phosphoserine By similarity
	Modified residue	462	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02356-1 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 3076B550E17AF95C	FASTA	824	94,787
	10 20 30 40 50 60 MASYPGPGKS KAKYPFKKRA SLQASAAAPE ARSGLGASPL QSARSLPGTA PCLKHFPLDL 70 80 90 100 110 120 RTSMDGKCKE IAEELFSRSL AESELRSAPY EFPEESPIEQ LEERRQRLER QISQDVKLEP 130 140 150 160 170 180 DILLRAKQDF LKTDSDSDLQ LYKEQGEGQG DRGLWERDVV LEREFQRVII SGEEKCGVPF 190 200 210 220 230 240 TDLLDAAKSV VRALFIREKY MALSLQSFCP TTRRYLQQLA EKPLETRTYE QSPDTPVSAD 250 260 270 280 290 300 APVHPPALEQ HPYEHCEPST MPGDLGLGLR MVRGVVHVYT RRDPDEHCPE VELPYPDLQE 310 320 330 340 350 360 FVADVNVLMA LIINGPIKSF CYRRLQYLSS KFQMHVLLNE MKELAAQKKV PHRDFYNIRK 370 380 390 400 410 420 VDTHIHASSC MNQKHLLRFI KRAMKRHLEE IVHVEQGREQ TLREVFESMN LTAYDLSVDT 430 440 450 460 470 480 LDVHADRNTF HRFDKFNAKY NPIGESVLRE IFIKTDNKIS GKYFAHIIKE VMSDLEESKY 490 500 510 520 530 540 QNAELRLSIY GRSRDEWDKL ARWAVNHRVH SPNVRWLVQV PRLFDVYRTK GQLANFQEML 550 560 570 580 590 600 ENIFLPLFEA TVHPASHPEL HLFLEHVDGF DSVDDESKPE NHVFNLESPL PEAWVEEDNP 610 620 630 640 650 660 PYAYYLYYTF ANMAMLNHLR RQRGFHTFVL RPHCGEAGPI HHLVSAFMLA ENISHGLLLR 670 680 690 700 710 720 KAPVLQYLYY LAQIGIAMSP LSNNSLFLSY HRNPLPEYLS RGLMVSLSTD DPLQFHFTKE 730 740 750 760 770 780 PLMEEYSIAT QVWKLSSCDM CELARNSVLM SGFSHKVKSH WLGPNYTKEG PEGNDIRRTN 790 800 810 820 VPDIRVGYRY ETLCQELALI TQAVQSEMLE TIPEEVGIVM SPGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[2]	"Adenylate deaminase. A multigene family in humans and rats." Morisaki T., Sabina R.L., Holmes E.W. J. Biol. Chem. 265:11482-11486(1990) [PubMed: 2365682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 632-719. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC166402 mRNA. Translation: AAI66402.1. M38126 Genomic DNA. Translation: AAA40728.1.
IPI	IPI00554244.
PIR	A37056.
RefSeq	NP_001095151.1.
UniGene	Rn.104557
3D structure databases
SMR	Q02356. Positions 1-88.
ModBase	Search...
Protein-protein interaction databases
STRING	Q02356.
PTM databases
PhosphoSite	Q02356.
Genome annotation databases
Ensembl	ENSRNOT00000026051; ENSRNOP00000026051; ENSRNOG00000019240; Rattus norvegicus. [Genome view] ENSRNOT00000051972; ENSRNOP00000050656; ENSRNOG00000019240; Rattus norvegicus. [Genome view]
GeneID	362015.
KEGG	rno:362015.
Organism-specific databases
CTD	362015.
RGD	2110. Ampd2.
Phylogenomic databases
HOVERGEN	Q02356.
Enzyme and pathway databases
BRENDA	3.5.4.6. 248.
Gene expression databases
ArrayExpress	Q02356.
Genevestigator	Q02356.
GermOnline	ENSRNOG00000019240. Rattus norvegicus.
Family and domain databases
InterPro	IPR006650. A/AMP_deam_AS. IPR001365. A/AMP_deaminase. IPR006329. AMP_deaminase. IPR016297. AMP_deaminase_met. [Graphical view]
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
PIRSF	PIRSF001251. AMP_deaminase_met. 1 hit.
TIGRFAMs	TIGR01429. AMP_deaminase. 1 hit.
PROSITE	PS00485. A_DEAMINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPD2_RAT

Accession

Primary (citable) accession number: Q02356
Secondary accession number(s): B2GUT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	March 24, 2009
Last modified:	October 13, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)