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Q02346 (MYOD1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myoblast determination protein 1
Gene names
Name:Myod1
Synonyms:Myod
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins By similarity.

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1 and CDK9. Interacts with DDX5. Interacts with CHD2 By similarity.

Subcellular location

Nucleus.

Post-translational modification

Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation By similarity.

Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function By similarity.

Ubiquitinated on the N-terminus; which is required for proteasomal degradation By similarity.

Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity By similarity.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processDifferentiation
Myogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   PTMAcetylation
Methylation
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to estradiol stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

histone H3 acetylation

Inferred from sequence or structural similarity. Source: UniProtKB

histone H4 acetylation

Inferred from sequence or structural similarity. Source: UniProtKB

myoblast differentiation

Inferred from sequence or structural similarity PubMed 12037571. Source: UniProtKB

myoblast fate determination

Inferred from sequence or structural similarity PubMed 12486129. Source: UniProtKB

positive regulation of skeletal muscle tissue regeneration

Inferred from sequence or structural similarity PubMed 17028574. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity PubMed 12486129. Source: UniProtKB

regulation of alternative mRNA splicing, via spliceosome

Inferred from sequence or structural similarity PubMed 9525963. Source: UniProtKB

skeletal muscle tissue development

Inferred from sequence or structural similarity PubMed 14762206. Source: UniProtKB

striated muscle cell differentiation

Inferred from sequence or structural similarity PubMed 17028574. Source: UniProtKB

transcription, DNA-templated

Inferred from sequence or structural similarity PubMed 15520228. Source: UniProtKB

   Cellular_componentnucleus

Inferred from sequence or structural similarity PubMed 11711431. Source: UniProtKB

   Molecular_functionchromatin DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

core promoter binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Myoblast determination protein 1
PRO_0000127363

Regions

Domain109 – 16052bHLH

Amino acid modifications

Modified residue1041N6-methyllysine; by EHMT2 By similarity
Cross-link1Peptide (Met-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q02346 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: FC43E46BB1287F5F

FASTA31834,359
        10         20         30         40         50         60 
MELLSPPLRD TDLLGPDGSL CSFATRDDFY DDPCFDSPDL RFFEDLDPRL VHVGALLKPE 

        70         80         90        100        110        120 
EHAHFPTTVH PGPGAREDEH VRAPSGHHQA GRCLLWACKA CKRKTTNADR RKAATMRERR 

       130        140        150        160        170        180 
RLSKVNEAFE TLKRCTSSNP NQRLPKVEIL RNAIRYIEGL QALLRDQDAA PPGAAAFYAP 

       190        200        210        220        230        240 
GPLPPGRGSE HYSGDSDASS PRSNCSDGMM DYSGPPSGPR RQNGYDAAYY SEASSEPRPG 

       250        260        270        280        290        300 
KSAAVSSLDC LSSIVERIST DSPAAPSLLL PDAPPESPPG PPEETSSSDA EQGTQTPSPD 

       310 
STPQCPAGSK PNPIYQVL 

« Hide

References

[1]"Isolation and structural analysis of the rat MyoD gene."
Vaidya T.B., Rhodes S.J., Moore J.L., Sherman D.A., Konieczny S.F., Taparowsky E.J.
Gene 116:223-230(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84176 Genomic DNA. Translation: AAA41661.1.
PIRJC1171.
RefSeqNP_788268.1. NM_176079.1.
UniGeneRn.9493.

3D structure databases

ProteinModelPortalQ02346.
SMRQ02346. Positions 102-166.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ02346.

Proteomic databases

PaxDbQ02346.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID337868.
KEGGrno:337868.
UCSCRGD:631429. rat.

Organism-specific databases

CTD4654.
RGD631429. Myod1.

Phylogenomic databases

eggNOGNOG292792.
HOGENOMHOG000234800.
HOVERGENHBG006429.
InParanoidQ02346.
KOK09064.
PhylomeDBQ02346.

Gene expression databases

GenevestigatorQ02346.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR002546. Basic.
IPR011598. bHLH_dom.
IPR022032. Myf5.
[Graphical view]
PfamPF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
PF12232. Myf5. 1 hit.
[Graphical view]
SMARTSM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio672553.
PROQ02346.

Entry information

Entry nameMYOD1_RAT
AccessionPrimary (citable) accession number: Q02346
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families