Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myoblast determination protein 1

Gene

Myod1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).By similarity

GO - Molecular functioni

  1. chromatin DNA binding Source: UniProtKB
  2. core promoter binding Source: UniProtKB
  3. E-box binding Source: RGD
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: GO_Central
  5. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: GO_Central
  6. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: GO_Central

GO - Biological processi

  1. cellular response to estradiol stimulus Source: UniProtKB
  2. histone H3 acetylation Source: UniProtKB
  3. histone H4 acetylation Source: UniProtKB
  4. myoblast differentiation Source: UniProtKB
  5. myoblast fate determination Source: UniProtKB
  6. positive regulation of myoblast differentiation Source: GO_Central
  7. positive regulation of myoblast fusion Source: GO_Central
  8. positive regulation of skeletal muscle fiber development Source: GO_Central
  9. positive regulation of skeletal muscle tissue regeneration Source: UniProtKB
  10. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  11. regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  12. response to muscle stretch Source: RGD
  13. skeletal muscle atrophy Source: RGD
  14. skeletal muscle cell differentiation Source: GO_Central
  15. skeletal muscle tissue development Source: UniProtKB
  16. skeletal muscle tissue regeneration Source: RGD
  17. striated muscle cell differentiation Source: UniProtKB
  18. transcription, DNA-templated Source: UniProtKB
  19. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Myogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myoblast determination protein 1
Gene namesi
Name:Myod1
Synonyms:Myod
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi631429. Myod1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Myoblast determination protein 1PRO_0000127363Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki1 – 1Peptide (Met-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei104 – 1041N6-methyllysine; by EHMT2By similarity

Post-translational modificationi

Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation (By similarity).By similarity
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).By similarity
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.By similarity
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ02346.

PTM databases

PhosphoSiteiQ02346.

Expressioni

Gene expression databases

GenevestigatoriQ02346.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1 and CDK9. Interacts with DDX5. Interacts with CHD2. Interacts with TSC22D3 (By similarity). Interacts with SETD3 (By similarity).By similarity

Structurei

3D structure databases

SMRiQ02346. Positions 102-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini109 – 16052bHLHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG292792.
HOGENOMiHOG000234800.
HOVERGENiHBG006429.
InParanoidiQ02346.
KOiK09064.
PhylomeDBiQ02346.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR002546. Basic.
IPR011598. bHLH_dom.
IPR022032. Myf5.
[Graphical view]
PfamiPF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
PF12232. Myf5. 1 hit.
[Graphical view]
SMARTiSM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02346-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLSPPLRD TDLLGPDGSL CSFATRDDFY DDPCFDSPDL RFFEDLDPRL
60 70 80 90 100
VHVGALLKPE EHAHFPTTVH PGPGAREDEH VRAPSGHHQA GRCLLWACKA
110 120 130 140 150
CKRKTTNADR RKAATMRERR RLSKVNEAFE TLKRCTSSNP NQRLPKVEIL
160 170 180 190 200
RNAIRYIEGL QALLRDQDAA PPGAAAFYAP GPLPPGRGSE HYSGDSDASS
210 220 230 240 250
PRSNCSDGMM DYSGPPSGPR RQNGYDAAYY SEASSEPRPG KSAAVSSLDC
260 270 280 290 300
LSSIVERIST DSPAAPSLLL PDAPPESPPG PPEETSSSDA EQGTQTPSPD
310
STPQCPAGSK PNPIYQVL
Length:318
Mass (Da):34,359
Last modified:July 1, 1993 - v1
Checksum:iFC43E46BB1287F5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84176 Genomic DNA. Translation: AAA41661.1.
PIRiJC1171.
RefSeqiNP_788268.1. NM_176079.1.
UniGeneiRn.9493.

Genome annotation databases

GeneIDi337868.
KEGGirno:337868.
UCSCiRGD:631429. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84176 Genomic DNA. Translation: AAA41661.1.
PIRiJC1171.
RefSeqiNP_788268.1. NM_176079.1.
UniGeneiRn.9493.

3D structure databases

SMRiQ02346. Positions 102-166.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ02346.

Proteomic databases

PaxDbiQ02346.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi337868.
KEGGirno:337868.
UCSCiRGD:631429. rat.

Organism-specific databases

CTDi4654.
RGDi631429. Myod1.

Phylogenomic databases

eggNOGiNOG292792.
HOGENOMiHOG000234800.
HOVERGENiHBG006429.
InParanoidiQ02346.
KOiK09064.
PhylomeDBiQ02346.

Miscellaneous databases

NextBioi672553.
PROiQ02346.

Gene expression databases

GenevestigatoriQ02346.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR002546. Basic.
IPR011598. bHLH_dom.
IPR022032. Myf5.
[Graphical view]
PfamiPF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
PF12232. Myf5. 1 hit.
[Graphical view]
SMARTiSM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMYOD1_RAT
AccessioniPrimary (citable) accession number: Q02346
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 29, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.