Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myoblast determination protein 1

Gene

Myod1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to estradiol stimulus Source: UniProtKB
  • histone H3 acetylation Source: UniProtKB
  • histone H4 acetylation Source: UniProtKB
  • myoblast differentiation Source: UniProtKB
  • myoblast fate determination Source: UniProtKB
  • positive regulation of myoblast differentiation Source: GO_Central
  • positive regulation of myoblast fusion Source: GO_Central
  • positive regulation of skeletal muscle fiber development Source: GO_Central
  • positive regulation of skeletal muscle tissue regeneration Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  • response to muscle stretch Source: RGD
  • skeletal muscle atrophy Source: RGD
  • skeletal muscle cell differentiation Source: GO_Central
  • skeletal muscle tissue development Source: UniProtKB
  • skeletal muscle tissue regeneration Source: RGD
  • striated muscle cell differentiation Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Myogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Myoblast determination protein 1
Gene namesi
Name:Myod1
Synonyms:Myod
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi631429. Myod1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001273631 – 318Myoblast determination protein 1Add BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki1Peptide (Met-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei104N6-methyllysine; by EHMT2By similarity1

Post-translational modificationi

Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation (By similarity).By similarity
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).By similarity
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.By similarity
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ02346.

PTM databases

PhosphoSitePlusiQ02346.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1. Interacts with DDX5. Interacts with CHD2. Interacts with TSC22D3 (By similarity). Interacts with SETD3 (By similarity). Interacts with P-TEFB complex; promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation (By similarity). Interacts with CSRP3 (PubMed:9234731).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015109.

Structurei

3D structure databases

ProteinModelPortaliQ02346.
SMRiQ02346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 160bHLHPROSITE-ProRule annotationAdd BLAST52

Phylogenomic databases

eggNOGiKOG3960. Eukaryota.
ENOG4111SED. LUCA.
HOGENOMiHOG000234800.
HOVERGENiHBG006429.
InParanoidiQ02346.
KOiK09064.
PhylomeDBiQ02346.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR002546. Basic.
IPR011598. bHLH_dom.
IPR022032. Myf5.
PfamiView protein in Pfam
PF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
PF12232. Myf5. 1 hit.
SMARTiView protein in SMART
SM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02346-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLSPPLRD TDLLGPDGSL CSFATRDDFY DDPCFDSPDL RFFEDLDPRL
60 70 80 90 100
VHVGALLKPE EHAHFPTTVH PGPGAREDEH VRAPSGHHQA GRCLLWACKA
110 120 130 140 150
CKRKTTNADR RKAATMRERR RLSKVNEAFE TLKRCTSSNP NQRLPKVEIL
160 170 180 190 200
RNAIRYIEGL QALLRDQDAA PPGAAAFYAP GPLPPGRGSE HYSGDSDASS
210 220 230 240 250
PRSNCSDGMM DYSGPPSGPR RQNGYDAAYY SEASSEPRPG KSAAVSSLDC
260 270 280 290 300
LSSIVERIST DSPAAPSLLL PDAPPESPPG PPEETSSSDA EQGTQTPSPD
310
STPQCPAGSK PNPIYQVL
Length:318
Mass (Da):34,359
Last modified:July 1, 1993 - v1
Checksum:iFC43E46BB1287F5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84176 Genomic DNA. Translation: AAA41661.1.
PIRiJC1171.
RefSeqiNP_788268.1. NM_176079.1.
UniGeneiRn.9493.

Genome annotation databases

GeneIDi337868.
KEGGirno:337868.
UCSCiRGD:631429. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMYOD1_RAT
AccessioniPrimary (citable) accession number: Q02346
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 10, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome