Reviewed, UniProtKB/Swiss-Prot Q02337 (BDH_BOVIN)

Names and origin

Protein names

Recommended name:
    D-beta-hydroxybutyrate dehydrogenase, mitochondrial
      Short name=BDH
    EC=1.1.1.30
Alternative name(s):
    3-hydroxybutyrate dehydrogenase

Gene names

Name:	BDH1
Synonyms:	BDH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH.
Enzyme regulation	Requires phosphatidylcholine as an allosteric activator for enzymic activity.
Subunit structure	Homotetramer.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Allosteric enzyme Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxybutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Mitochondrion
	Chain	47 – 344	298	D-beta-hydroxybutyrate dehydrogenase, mitochondrial		PRO_0000054526
Regions
	Nucleotide binding	59 – 83	25	NAD By similarity
Sites
	Active site	208	1	Proton acceptor By similarity
	Binding site	195	1	Substrate By similarity
Amino acid modifications
	Modified residue	276	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02337-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: C8C7AC75FA09880E	FASTA	344	38,391
	10 20 30 40 50 60 MLTARLSRPL SQLPRKTLNF SDRENGTRGS LLLYSAPFVP VGRRTYAASV DPVGSKAVLI 70 80 90 100 110 120 TGCDSGFGFS LAKHLHSEGF LVFAGCLMKD KGSDGVKELD SMKSDRLRTV QLNVCKSEEV 130 140 150 160 170 180 DKAAEVIRSS LEDPEKGLWG LVNNAGISTF GDVEFTSMET YKEVAEVNLW GTVRVTKAFL 190 200 210 220 230 240 PLIRRAKGRV VNISSMMGRM ANVARSPYCI TKFGVEAFSD CLRYEMHPLG VKVSVVEPGN 250 260 270 280 290 300 FIAATSLYGG TERIQAIANK MWEELPEVVR QDYGRKYFDE KVARMESYCT SGSTDTSPVI 310 320 330 340 KAVTHALTAT TPYTRYHPMD YYWWLRMQIM THFPGAISDR IYIH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.
[2]	"Molecular cloning and characterization of (R)-3-hydroxybutyrate dehydrogenase from human heart." Marks A.R., McIntyre J.O., Duncan T.M., Erdjument-Bromage H., Tempst P., Fleischer S. J. Biol. Chem. 267:15459-15463(1992) [PubMed: 1639787] [Abstract] Cited for: PROTEIN SEQUENCE OF 47-89; 138-174; 213-231; 233-259; 285-322 AND 331-344. Tissue: Heart.
[3]	"Amino acid sequences of two tryptic peptides from D(-)-beta-hydroxybutyrate dehydrogenase radiolabeled at essential carboxyl and sulfhydryl groups." Prasad P.V., Hatefi Y. Biochem. Int. 12:941-949(1986) [PubMed: 3527172] [Abstract] Cited for: PROTEIN SEQUENCE OF 163-174 AND 285-301. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC103051 mRNA. Translation: AAI03052.1.
PIR	B42845.
RefSeq	NP_001029772.1.
UniGene	Bt.49607
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000000448. Bos taurus. [Contig view]
GeneID	534090.
KEGG	bta:534090.
Phylogenomic databases
HOVERGEN	Q02337.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BDH_BOVIN

Accession

Primary (citable) accession number: Q02337
Secondary accession number(s): Q3ZBX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	July 25, 2006
Last modified:	November 25, 2008
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)