Reviewed, UniProtKB/Swiss-Prot Q02332 (PDK_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable [pyruvate dehydrogenase [lipoamide]] kinase, mitochondrial
      Short name=Pyruvate dehydrogenase kinase
    EC=2.7.11.2

Gene names

ORF Names:

ZK370.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism By similarity.
Catalytic activity	ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate dehydrogenase (acetyl-transferring) kinase activity Inferred from electronic annotation. Source: EC two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 401		Probable [pyruvate dehydrogenase [lipoamide]] kinase, mitochondrial		PRO_0000023450
Regions
	Domain	131 – 360	230	Histidine kinase

Sequences

	Sequence		Length	Mass (Da)
	Q02332-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 00754E7999BACC12	FASTA	401	45,282
	10 20 30 40 50 60 MRFSRKLLGP FVGSLAKKLD YYSQFQPSSL TIQQYLDFGR IGTSANSYTF LKNELLVRLA 70 80 90 100 110 120 NIMQEFTLLP PKLLQMPSSK MVSNWYAESF EDLLLFEASD SSPEQVARFN DQLTVVLKRH 130 140 150 160 170 180 AHVVETMAEG LIELRESDGV DIASEKGIQY FLDRFYINRI SIRMLQNQHL VVFGNVLPES 190 200 210 220 230 240 PRHVGCIDPA CDVESVVYDA FENARFLCDR YYLTSPSMKL EMHNAVEKGK PISIVAVPSH 250 260 270 280 290 300 LYHMMFELFK NAMRATVEYH GVDDDLPDIK VYVVKGQEDL SIKICDRGGG VSRTILERLY 310 320 330 340 350 360 NYMYSTAPPP PRDGTQAPLA GYGYGLPLSR LYARYFLGDL FLVSMEGHGT DACIYLKAVP 370 380 390 400 VEASEVLPIY STSSRRNLTM GPQVADWSHH VPGQGNRPAQ S

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M98552 Genomic DNA. Translation: AAA28207.1.
PIR	S44666.
RefSeq	NP_498928.1.
UniGene	Cel.9569
3D structure databases
HSSP	HSSP built from PDB template 1JM6 based on UniProtKB Q64536.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:26171N.
IntAct	Q02332. 1 interaction.
STRING	Q02332.
Genome annotation databases
Ensembl	ZK370.5.1; ZK370.5.1; ZK370.5; Caenorhabditis elegans. [Genome view]
GeneID	176226.
KEGG	cel:ZK370.5.
Organism-specific databases
CTD	176226.
WormBase	WBGene00022719. ZK370.5.
WormPep	ZK370.5. CE00397. [WorfDB]
Phylogenomic databases
OMA	APNKPIQ.
Enzyme and pathway databases
BRENDA	2.7.11.2. 672.
Gene expression databases
ArrayExpress	Q02332.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR018955. BCDHK/PDK_mit. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF10436. BCDHK_Adom3. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	891656.

Entry information

Entry name

PDK_CAEEL

Accession

Primary (citable) accession number: Q02332

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project