Reviewed, UniProtKB/Swiss-Prot Q02326 (RL6A_YEAST)

Names and origin

Protein names

Recommended name:
    60S ribosomal protein L6-A
Alternative name(s):
    L17
    YL16
    RP18

Gene names

Name:	RPL6A
Synonyms:	RPL17A, YL16A
Ordered Locus Names:	YML073C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.3
Subcellular location	Cytoplasm.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Miscellaneous	Present with 37100 molecules/cell in log phase SD medium. Ref.6 There are 2 genes for L6 in yeast.
Sequence similarities	Belongs to the ribosomal protein L6e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ribosomal large subunit assembly Inferred from mutant phenotype. Source: SGD translation Inferred from direct assay. Source: SGD
Cellular component	cytosolic large ribosomal subunit Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from direct assay. Source: SGD structural constituent of ribosome Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 176	175	60S ribosomal protein L6-A		PRO_0000171017
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.4
	Modified residue	12	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Modified residue	162	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q02326-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2E0AB1810D3AE121	FASTA	176	19,962
	10 20 30 40 50 60 MSAQKAPKWY PSEDVAALKK TRKAARPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED 70 80 90 100 110 120 NTLLISGPFK VNGVPLRRVN ARYVIATSTK VSVEGVNVEK FNVEYFAKEK LTKKEKKEAN 130 140 150 160 170 LFPEQQNKEI KAERVEDQKV VDKALIAEIK KTPLLKQYLS ASFSLKNGDK PHMLKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast ribosomal proteins: XIV. Complete nucleotide sequences of the two genes encoding Saccharomyces cerevisiae YL16." Hashimoto T., Suzuki K., Mizuta K., Otaka E. Biochim. Biophys. Acta 1132:195-198(1992) [PubMed: 1390890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[4]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12 AND SER-162, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10225 Genomic DNA. Translation: BAA01077.1. Z46373 Genomic DNA. Translation: CAA86505.1.
PIR	S28944.
RefSeq	NP_013638.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q02326. 37 interactions.
STRING	Q02326.
Proteomic databases
PeptideAtlas	Q02326.
PRIDE	Q02326.
Genome annotation databases
Ensembl	YML073C; YML073C; YML073C; Saccharomyces cerevisiae. [Genome view]
GeneID	854902.
KEGG	sce:YML073C.
NMPDR	fig|4932.3.peg.4675.
Organism-specific databases
CYGD	YML073c.
SGD	S000004538. RPL6A.
Phylogenomic databases
HOGENOM	Q02326.
OMA	LLKQYLA
OrthoDB	EOG9Q5B5Q
Gene expression databases
ArrayExpress	Q02326.
Genevestigator	Q02326.
GermOnline	YML073C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000915. Ribosomal_L6E. [Graphical view]
PANTHER	PTHR10715. Ribosomal_L6E. 1 hit.
Pfam	PF01159. Ribosomal_L6e. 1 hit. [Graphical view]
PROSITE	PS01170. RIBOSOMAL_L6E. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977882.

Entry information

Entry name

RL6A_YEAST

Accession

Primary (citable) accession number: Q02326

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)