Reviewed, UniProtKB/Swiss-Prot Q02315 (CP51_ISSOR)

Names and origin

Protein names

Recommended name:
    Lanosterol 14-alpha demethylase
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51
    CYPLI
    Cytochrome P450-LIA1
    Sterol 14-alpha demethylase
    Cytochrome P450-14DM

Gene names

Name:

CYP51

Organism

Issatchenkia orientalis (Yeast) (Candida krusei)

Taxonomic identifier

4909 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	414 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›414	›414	Lanosterol 14-alpha demethylase		PRO_0000052007
Experimental info
	Non-terminal residue	414	1

Sequences

	Sequence		Length	Mass (Da)
	Q02315-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: A23CCD0EE1F3A72D	FASTA	414	46,981
	10 20 30 40 50 60 MPWVGSAVVY GMQPYEFFEN CRKQHGDVFS FLLLGKVMTV YLGPKGHEFV LNAKLSDVSA 70 80 90 100 110 120 EDAYTHLTTP VFGKGVIYDC PNWKLMEQKK FAKVALTKES FIRYVPLIKD EMLKYFNANF 130 140 150 160 170 180 RGDSGKTDVL KSQSEMTLFT ASRSLFGDAL RNRLDASYAE MYSDLDKGFT PLNFVFSYLP 190 200 210 220 230 240 LPNYWKRDAA HKNISNTYLD LINTKRAGGE IKNEDLVDAL LKNSVYKDGT RMTDEELAHL 250 260 270 280 290 300 MIGVLMGGQH TSSATSAWFL LHLGEKPQLQ EEIYREIQSV LGENFERELT YDDLQKLDLV 310 320 330 340 350 360 NATIKETLRL HMPLHSIFRK VTRDLPVPNT SYIVPKGHYV LISPGYTMLS ERYFPNASEF 370 380 390 400 410 QPHRWDEIKS IDGGISFGAE GENAKETVDY GFGKISKGVA SPYLPFGGGR HRCT

References

[1]

"Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment." Burgener-Kairuz P., Zuber J.P., Jaunin P., Buchman T.G., Bille J., Rossier M. J. Clin. Microbiol. 32:1902-1907(1994) [PubMed: 7989540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6258 / CBS 573 / DSM 6128 / JCM 1609 / NBRC 1064.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S75391 Genomic DNA. Translation: AAB32680.1.
3D structure databases
SMR	Q02315. Positions 1-414.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.70. 290514.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_ISSOR

Accession

Primary (citable) accession number: Q02315

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)