Q02308 (HLES_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein hairless

Gene names

Name:	H
ORF Names:	CG5460

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1077 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is a potent antagonist of neurogenic gene activity during sensory organ development. The expression of distinct cell fates by the trichogen (shaft) / tormogen (socket) sister cell pair depends on the level of H activity. A certain threshold level of H activity is required, below which both sister cells adopt the tormogen fate. Ref.1 Ref.2
Subcellular location	Nucleus Probable.
Tissue specificity	During embryogenesis expression is primarily in endo- and mesodermal cell layers. Ovary, embryos, larval and pupal imaginal disks. Ref.1 Ref.2
Developmental stage	Expression peaks during embryogenesis and lowest during larval stages. Ref.2
Sequence caution	The sequence AAA28607.1 differs from that shown. Reason: Erroneous initiation. The sequence AAK93230.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Non-traceable author statement. Source: FlyBase imaginal disc-derived wing margin morphogenesis Inferred from mutant phenotype. Source: FlyBase imaginal disc-derived wing vein morphogenesis Inferred from mutant phenotype. Source: FlyBase negative regulation of Notch signaling pathway Traceable author statement. Source: FlyBase regulation of cell death Inferred from mutant phenotype. Source: FlyBase sensory organ precursor cell fate determination Inferred from mutant phenotype. Source: FlyBase wing disc dorsal/ventral pattern formation Inferred from mutant phenotype. Source: FlyBase
Cellular component	nucleus Traceable author statement. Source: FlyBase
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transcription corepressor activity Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q02308-1)

Also known as: D;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q02308-2)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1077	1077	Protein hairless		PRO_0000083990
Regions
	Compositional bias	115 – 123	9	Thr-rich
	Compositional bias	642 – 648	7	Poly-Ser
	Compositional bias	879 – 891	13	Poly-Ala
	Compositional bias	937 – 946	10	Poly-Ala
	Compositional bias	964 – 974	11	Ala-rich
	Compositional bias	979 – 1008	30	His/Pro-rich (PRD motif)
Amino acid modifications
	Modified residue	720	1	Phosphoserine Ref.7
	Modified residue	723	1	Phosphoserine Ref.7
	Modified residue	746	1	Phosphoserine Ref.7
	Modified residue	753	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 18	18	Missing in isoform B.		VSP_006952
Experimental info
	Sequence conflict	8	1	T → S in AAT94449. Ref.5
	Sequence conflict	680	1	F → S in CAA47664. Ref.2
	Sequence conflict	914	1	L → P in AAT94449. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (D) [UniParc]. Last modified September 23, 2008. Version 2. Checksum: E8B846C0E2E575D4	FASTA	1,077	111,100
	10 20 30 40 50 60 MALLNDVTSV AECNRQTTMT DEHKSNINSN SSHSSNNNNN GSSSNNDNNS NDDAASSSNS 70 80 90 100 110 120 KNNNTSNESS HSNNNTSSII AEAAAKFLLK NGLNGSSSTS YPPLPPPLPA NLSRTTTPTT 130 140 150 160 170 180 TTTPSSSSST ASNGFLPHAK TPKSSSIMAA SAAVAASVVG ATASKPTIDV LGGVLDYSSL 190 200 210 220 230 240 GGAATGSLPT TAVVAAAAGT AKIGKGSNSG GSFDMGRTPI STHGNNSWGG YGGRLQFFKD 250 260 270 280 290 300 GKFILELARS KDGDKSGWVS VTRKTFRPPS AATSATVTPT SAVTTAYPKN ENSTSLSFSD 310 320 330 340 350 360 DNSSIQSSPW QRDQPWKQSR PRRGISKELS LFFHRPRNST LGRAALRTAA RKRRRPHEPL 370 380 390 400 410 420 TTSEDQQPIF ATAIKAENGD DTLKAEAAEA VEIENVAVAD TTTNEIKIEK PDTIKGEDDA 430 440 450 460 470 480 ERLEKEPKKA VSDDSESKEA SPGQQVEPQP KDETVDVEMK MNTSEDEEPM TELPRITNAV 490 500 510 520 530 540 NGDLNGDLKA SIGKPKSKPK PKAKLSSIIQ KLIDSVPARL EQMSKTSAVI ASTTTSSDRI 550 560 570 580 590 600 GGGLSHALTH KVSPPSSATA AGRLVEYHTQ HVSPRKRILR EFEKVSLEDN GCVNNGSGGA 610 620 630 640 650 660 SSGGAGGKRS RAKGTSTSSP AGKASPMNLA PPQGKPSPSP GSSSSSTSPA TLSTQPTRLN 670 680 690 700 710 720 SSYSIHSLLG GSSGSGSSSF SSSGKKCGDH PAAIISNVHH PQHSMYQPSS SSYPRALLTS 730 740 750 760 770 780 PKSPDVSGSN GGGGKSPSHT GTKKRSPPYS AGSPVDYGHS FYRDPYAGAG RPSTSGSASQ 790 800 810 820 830 840 DLSPPRSSPA SPATTPRTVP KKTASIRREF ASPSASSSSC PSPGDRSASP PERRHMQQQP 850 860 870 880 890 900 HLQRSSPLHY YMYPPPPQVN GNGSAGSPTS APPTSNSSAA AVAAAAAAAA AYIPSPSIYN 910 920 930 940 950 960 PYISTLAALR HNPLWMHHYQ TGASPLLSPH PQPGGSAAAA AAAAAARLSP QSAYHAFAYN 970 980 990 1000 1010 1020 GVGAAVAAAA AAAAFGQPAP SPHTHPHLAH PHQHPHPAAL TTHHSPAHLA TPKLTDSSTD 1030 1040 1050 1060 1070 QMSATSSHRT ASTSPSSSSA SASSSAATSG ASSSAMFHTS SLRNEQSSDL PLNLSKH
	Isoform B (C) [UniParc]. Checksum: 0825DC8015CACEF5	FASTA	1,059	109,152
	10 20 30 40 50 60 MTDEHKSNIN SNSSHSSNNN NNGSSSNNDN NSNDDAASSS NSKNNNTSNE SSHSNNNTSS 70 80 90 100 110 120 IIAEAAAKFL LKNGLNGSSS TSYPPLPPPL PANLSRTTTP TTTTTPSSSS STASNGFLPH 130 140 150 160 170 180 AKTPKSSSIM AASAAVAASV VGATASKPTI DVLGGVLDYS SLGGAATGSL PTTAVVAAAA 190 200 210 220 230 240 GTAKIGKGSN SGGSFDMGRT PISTHGNNSW GGYGGRLQFF KDGKFILELA RSKDGDKSGW 250 260 270 280 290 300 VSVTRKTFRP PSAATSATVT PTSAVTTAYP KNENSTSLSF SDDNSSIQSS PWQRDQPWKQ 310 320 330 340 350 360 SRPRRGISKE LSLFFHRPRN STLGRAALRT AARKRRRPHE PLTTSEDQQP IFATAIKAEN 370 380 390 400 410 420 GDDTLKAEAA EAVEIENVAV ADTTTNEIKI EKPDTIKGED DAERLEKEPK KAVSDDSESK 430 440 450 460 470 480 EASPGQQVEP QPKDETVDVE MKMNTSEDEE PMTELPRITN AVNGDLNGDL KASIGKPKSK 490 500 510 520 530 540 PKPKAKLSSI IQKLIDSVPA RLEQMSKTSA VIASTTTSSD RIGGGLSHAL THKVSPPSSA 550 560 570 580 590 600 TAAGRLVEYH TQHVSPRKRI LREFEKVSLE DNGCVNNGSG GASSGGAGGK RSRAKGTSTS 610 620 630 640 650 660 SPAGKASPMN LAPPQGKPSP SPGSSSSSTS PATLSTQPTR LNSSYSIHSL LGGSSGSGSS 670 680 690 700 710 720 SFSSSGKKCG DHPAAIISNV HHPQHSMYQP SSSSYPRALL TSPKSPDVSG SNGGGGKSPS 730 740 750 760 770 780 HTGTKKRSPP YSAGSPVDYG HSFYRDPYAG AGRPSTSGSA SQDLSPPRSS PASPATTPRT 790 800 810 820 830 840 VPKKTASIRR EFASPSASSS SCPSPGDRSA SPPERRHMQQ QPHLQRSSPL HYYMYPPPPQ 850 860 870 880 890 900 VNGNGSAGSP TSAPPTSNSS AAAVAAAAAA AAAYIPSPSI YNPYISTLAA LRHNPLWMHH 910 920 930 940 950 960 YQTGASPLLS PHPQPGGSAA AAAAAAAARL SPQSAYHAFA YNGVGAAVAA AAAAAAFGQP 970 980 990 1000 1010 1020 APSPHTHPHL AHPHQHPHPA ALTTHHSPAH LATPKLTDSS TDQMSATSSH RTASTSPSSS 1030 1040 1050 SASASSSAAT SGASSSAMFH TSSLRNEQSS DLPLNLSKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila gene Hairless encodes a novel basic protein that controls alternative cell fates in adult sensory organ development." Bang A.G., Posakony J.W. Genes Dev. 6:1752-1769(1992) [PubMed: 1516831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY. Tissue: Imaginal disk.
[2]	"Hairless, a Drosophila gene involved in neural development, encodes a novel, serine rich protein." Maier D., Stumm G., Kuhn K., Preiss A. Mech. Dev. 38:143-156(1992) [PubMed: 1419850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[6]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 265-1077. Strain: Berkeley. Tissue: Embryo.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-720; SER-723; SER-746 AND SER-753, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95192 mRNA. Translation: AAA28607.1. Different initiation. X67239 mRNA. Translation: CAA47664.1. AE014297 Genomic DNA. Translation: AAF55790.1. AE014297 Genomic DNA. Translation: AAF55791.1. AE014297 Genomic DNA. Translation: AAN14357.1. AE014297 Genomic DNA. Translation: AAN14358.1. BT015220 mRNA. Translation: AAT94449.1. AY051806 mRNA. Translation: AAK93230.1. Different initiation.
PIR	A44067.
RefSeq	NP_524418.2. NM_079694.2. NP_732533.1. NM_169907.1. NP_732534.1. NM_169908.1. NP_732535.1. NM_169909.1.
UniGene	Dm.7139.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q02308. 2 interactions.
MINT	MINT-288923.
STRING	Q02308.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0083914; FBpp0083322; FBgn0001169. FBtr0083916; FBpp0083324; FBgn0001169.
GeneID	42445.
KEGG	dme:Dmel_CG5460.
UCSC	CG5460-RA. d. melanogaster.
Organism-specific databases
FlyBase	FBgn0001169. H.
Phylogenomic databases
eggNOG	inNOG11281.
GeneTree	EMGT00050000000273.
InParanoid	Q02308.
OMA	RTVPKKT.
PhylomeDB	Q02308.
Gene expression databases
ArrayExpress	Q02308.
Bgee	Q02308.
GermOnline	CG5460. Drosophila melanogaster.
Family and domain databases
KO	K06064.
ProtoNet	Search...
Other
NextBio	828814.

Entry information

Entry name

HLES_DROME

Accession

Primary (citable) accession number: Q02308
Secondary accession number(s): A4V358 , A4V359, Q6AWM8, Q960W5, Q9VDK0, Q9VDK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project