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Q02294

- CAC1B_RAT

UniProt

Q02294 - CAC1B_RAT

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Protein
Voltage-dependent N-type calcium channel subunit alpha-1B
Gene
Cacna1b, Cach5, Cacnl1a5
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei314 – 3141Calcium ion selectivity and permeability By similarity
Sitei664 – 6641Calcium ion selectivity and permeability By similarity
Sitei1367 – 13671Calcium ion selectivity and permeability By similarity
Sitei1655 – 16551Calcium ion selectivity and permeability By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi452 – 4598ATP Reviewed prediction
Calcium bindingi1737 – 174812 By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. high voltage-gated calcium channel activity Source: RGD
  4. protein binding Source: UniProtKB
  5. voltage-gated calcium channel activity Source: RGD

GO - Biological processi

  1. calcium ion import Source: RGD
  2. calcium ion transport Source: RGD
  3. membrane depolarization during action potential Source: RefGenome
  4. positive regulation of neurotransmitter secretion Source: RGD
  5. response to ethanol Source: RGD
  6. response to organic cyclic compound Source: RGD
  7. response to testosterone Source: RGD
  8. sensory perception of pain Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent N-type calcium channel subunit alpha-1B
Alternative name(s):
Brain calcium channel III
Short name:
BIII
Calcium channel, L type, alpha-1 polypeptide isoform 5
Voltage-gated calcium channel subunit alpha Cav2.2
Gene namesi
Name:Cacna1b
Synonyms:Cach5, Cacnl1a5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi628852. Cacna1b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9595Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei96 – 11419Helical; Name=S1 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini115 – 13319Extracellular Reviewed prediction
Add
BLAST
Transmembranei134 – 15118Helical; Name=S2 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini152 – 16413Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei165 – 17915Helical; Name=S3 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini180 – 1867Extracellular Reviewed prediction
Transmembranei187 – 20519Helical; Name=S4 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini206 – 22520Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei226 – 24520Helical; Name=S5 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini246 – 33186Extracellular Reviewed prediction
Add
BLAST
Transmembranei332 – 35625Helical; Name=S6 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini357 – 483127Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei484 – 50320Helical; Name=S1 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini504 – 51714Extracellular Reviewed prediction
Add
BLAST
Transmembranei518 – 53720Helical; Name=S2 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini538 – 5458Cytoplasmic Reviewed prediction
Transmembranei546 – 56419Helical; Name=S3 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini565 – 57511Extracellular Reviewed prediction
Add
BLAST
Transmembranei576 – 59318Helical; Name=S4 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini594 – 61219Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei613 – 63220Helical; Name=S5 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini633 – 68553Extracellular Reviewed prediction
Add
BLAST
Transmembranei686 – 71025Helical; Name=S6 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini711 – 1143433Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1144 – 116724Helical; Name=S1 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1168 – 118417Extracellular Reviewed prediction
Add
BLAST
Transmembranei1185 – 120420Helical; Name=S2 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1205 – 12128Cytoplasmic Reviewed prediction
Transmembranei1213 – 123523Helical; Name=S3 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1236 – 125015Extracellular Reviewed prediction
Add
BLAST
Transmembranei1251 – 126515Helical; Name=S4 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1266 – 128621Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1287 – 130620Helical; Name=S5 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1307 – 139286Extracellular Reviewed prediction
Add
BLAST
Transmembranei1393 – 141725Helical; Name=S6 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1418 – 147457Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1475 – 149319Helical; Name=S1 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1494 – 150714Extracellular Reviewed prediction
Add
BLAST
Transmembranei1508 – 152720Helical; Name=S2 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1528 – 15369Cytoplasmic Reviewed prediction
Transmembranei1537 – 155519Helical; Name=S3 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1556 – 15638Extracellular Reviewed prediction
Transmembranei1564 – 158219Helical; Name=S4 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1583 – 160119Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1602 – 162120Helical; Name=S5 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1622 – 168362Extracellular Reviewed prediction
Add
BLAST
Transmembranei1684 – 170320Helical; Name=S6 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1704 – 2336633Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. axon terminus Source: RGD
  2. dendritic shaft Source: RGD
  3. neuron projection Source: RGD
  4. neuronal cell body Source: RGD
  5. plasma membrane Source: RGD
  6. protein complex Source: RGD
  7. voltage-gated calcium channel complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23362336Voltage-dependent N-type calcium channel subunit alpha-1B
PRO_0000053924Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi256 – 2561N-linked (GlcNAc...) Reviewed prediction
Modified residuei784 – 7841Phosphoserine By similarity
Glycosylationi1563 – 15631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1675 – 16751N-linked (GlcNAc...) Reviewed prediction
Modified residuei1719 – 17191Phosphoserine; by PKA Reviewed prediction

Post-translational modificationi

Phosphorylated in vitro by CaM-kinase II, PKA, PKC and CGPK.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ02294.
PRIDEiQ02294.

PTM databases

PhosphoSiteiQ02294.

Expressioni

Tissue specificityi

Central nervous system.

Gene expression databases

GenevestigatoriQ02294.

Interactioni

Subunit structurei

Multisubunit complex consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with RIMS1 and RIMBP2 By similarity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Syt1P217072EBI-540038,EBI-458098

Protein-protein interaction databases

IntActiQ02294. 4 interactions.
MINTiMINT-102470.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi364 – 40340

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DEXX-ray2.00B358-468[»]
ProteinModelPortaliQ02294.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati82 – 359278I
Add
BLAST
Repeati469 – 713245II
Add
BLAST
Repeati1135 – 1421287III
Add
BLAST
Repeati1458 – 1711254IV
Add
BLAST
Domaini1724 – 175936EF-hand
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni379 – 39618Binding to the beta subunit
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2049 – 20535Poly-His
Compositional biasi2115 – 21195Poly-Ser

Domaini

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.1 Publication

Sequence similaritiesi

Contains 1 EF-hand domain.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
HOGENOMiHOG000231530.
HOVERGENiHBG050763.
PhylomeDBiQ02294.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR002048. EF_hand_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR005447. VDCC_N_a1su.
IPR002077. VDCCAlpha1.
[Graphical view]
PfamiPF08763. Ca_chan_IQ. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01631. NVDCCALPHA1.
SMARTiSM01062. Ca_chan_IQ. 1 hit.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: 2 isoforms may be produced.

Isoform 1 (identifier: Q02294-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MVRFGDELGG RYGGTGGGER ARGGGAGGAG GPGQGGLPPG QRVLYKQSIA     50
QRARTMALYN PIPVKQNCFT VNRSLFVFSE DNVVRKYAKR ITEWPPFEYM 100
ILATIIANCI VLALEQHLPD GDKTPMSERL DDTEPYFIGI FCFEAGIKII 150
ALGFVFHKGS YLRNGWNVMD FVVVLTEILA TAGTDFDLRT LRAVRVLRPL 200
KLVSGIPSLQ VVLKSIMKAM VPLLQIGLLL FFAILMFAII GLEFYMGKFH 250
KACFPNSTDA EPVGDFPCGK EAPARLCDSD TECREYWPGP NFGITNFDNI 300
LFAILTVFQC ITMEGWTDIL YNTNDAAGNT WNWLYFIPLI IIGSFFMLNL 350
VLGVLSGEFA KERERVENRR AFLKLRRQQQ IERELNGYLE WIFKAEEVML 400
AEEDKNAEEK SPLDAVLKRA ATKKSRNDLI HAEEGEDRFV DLCAAGSPFA 450
RASLKSGKTE SSSYFRRKEK MFRFLIRRMV KAQSFYWVVL CVVALNTLCV 500
AMVHYNQPQR LTTALYFAEF VFLGLFLTEM SLKMYGLGPR SYFRSSFNCF 550
DFGVIVGSIF EVVWAAIKPG TSFGISVLRA LRLLRIFKVT KYWNSLRNLV 600
VSLLNSMKSI ISLLFLLFLF IVVFALLGMQ LFGGQFNFQD ETPTTNFDTF 650
PAAILTVFQI LTGEDWNAVM YHGIESQGGV SKGMFSSFYF IVLTLFGNYT 700
LLNVFLAIAV DNLANAQELT KDEEEMEEAA NQKLALQKAK EVAEVSPMSA 750
ANISIAARQQ NSAKARSVWE QRASQLRLQN LRASCEALYS EMDPEERLRY 800
ASTRHVRPDM KTHMDRPLVV EPGRDGLRGP AGNKSKPEGT EATEGADPPR 850
RHHRHRDRDK TSASTPAGGE QDRTDCPKAE STETGAREER ARPRRSHSKE 900
APGADTQVRC ERSRRHHRRG SPEEATEREP RRHRAHRHAQ DSSKEGKEGT 950
APVLVPKGER RARHRGPRTG PRETENSEEP TRRHRAKHKV PPTLEPPERE 1000
VAEKESNVVE GDKETRNHQP KEPRCDLEAI AVTGVGSLHM LPSTCLQKVD 1050
EQPEDADNQR NVTRMGSQPS DPSTTVHVPV TLTGPPGEAT VVPSANTDLE 1100
GQAEGKKEAE ADDVLRRGPR PIVPYSSMFC LSPTNLLRRF CHYIVTMRYF 1150
EMVILVVIAL SSIALAAEDP VRTDSFRNNA LKYMDYIFTG VFTFEMVIKM 1200
IDLGLLLHPG AYFRDLWNIL DFIVVSGALV AFAFSSFMGG SKGKDINTIK 1250
SLRVLRVLRP LKTIKRLPKL KAVFDCVVNS LKNVLNILIV YMLFMFIFAV 1300
IAVQLFKGKF FYCTDESKEL ERDCRGQYLD YEKEEVEAQP RQWKKYDFHY 1350
DNVLWALLTL FTVSTGEGWP MVLKHSVDAT YEEQGPSPGF RMELSIFYVV 1400
YFVVFPFFFV NIFVALIIIT FQEQGDKVMS ECSLEKNERA CIDFAISAKP 1450
LTRYMPQNKQ SFQYKTWTFV VSPPFEYFIM AMIALNTVVL MMKFYDAPYE 1500
YELMLKCLNI VFTSMFSLEC ILKIIAFGVL NYFRDAWNVF DFVTVLGSIT 1550
DILVTEIANN FINLSFLRLF RAARLIKLCR QGYTIRILLW TFVQSFKALP 1600
YVCLLIAMLF FIYAIIGMQV FGNIALDDGT SINRHNNFRT FLQALMLLFR 1650
SATGEAWHEI MLSCLGNRAC DPHANASECG SDFAYFYFVS FIFLCSFLML 1700
NLFVAVIMDN FEYLTRDSSI LGPHHLDEFI RVWAEYDPAA CGRISYNDMF 1750
EMLKHMSPPL GLGKKCPARV AYKRLVRMNM PISNEDMTVH FTSTLMALIR 1800
TALEIKLAPA GTKQHQCDAE LRKEISSVWA NLPQKTLDLL VPPHKPDEMT 1850
VGKVYAALMI FDFYKQNKTT RDQTHQAPGG LSQMGPVSLF HPLKATLEQT 1900
QPAVLRGARV FLRQKSATSL SNGGAIQTQE SGIKESLSWG TQRTQDVLYE 1950
ARAPLERGHS AEIPVGQPGA LAVDVQMQNM TLRGPDGEPQ PGLESQGRAA 2000
SMPRLAAETQ PAPNASPMKR SISTLAPRPH GTQLCNTVLD RPPPSQVSHH 2050
HHHRCHRRRD KKQRSLEKGP SLSVDTEGAP STAAGSGLPH GEGSTGCRRE 2100
RKQERGRSQE RRQPSSSSSE KQRFYSCDRF GSREPPQPKP SLSSHPISPT 2150
AALEPGPHPQ GSGSVNGSPL MSTSGASTPG RGGRRQLPQT PLTPRPSITY 2200
KTANSSPVHF AEGQSGLPAF SPGRLSRGLS EHNALLQKEP LSQPLASGSR 2250
IGSDPYLGQR LDSEASAHNL PEDTLTFEEA VATNSGRSSR TSYVSSLTSQ 2300
SHPLRRVPNG YHCTLGLSTG VRARHSYHHP DQDHWC 2336
Length:2,336
Mass (Da):262,256
Last modified:October 1, 1996 - v1
Checksum:i8D50AF67834FD1BC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1538 – 15381N → D no nucleotide entry 1 Publication
Sequence conflicti1579 – 15791C → L no nucleotide entry 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M92905 mRNA. Translation: AAA42014.1.
PIRiB35901.
UniGeneiRn.85880.

Genome annotation databases

UCSCiRGD:628852. rat. [Q02294-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M92905 mRNA. Translation: AAA42014.1 .
PIRi B35901.
UniGenei Rn.85880.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4DEX X-ray 2.00 B 358-468 [» ]
ProteinModelPortali Q02294.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q02294. 4 interactions.
MINTi MINT-102470.

Chemistry

BindingDBi Q02294.
ChEMBLi CHEMBL5107.
GuidetoPHARMACOLOGYi 533.

PTM databases

PhosphoSitei Q02294.

Proteomic databases

PaxDbi Q02294.
PRIDEi Q02294.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:628852. rat. [Q02294-1 ]

Organism-specific databases

RGDi 628852. Cacna1b.

Phylogenomic databases

eggNOGi COG1226.
HOGENOMi HOG000231530.
HOVERGENi HBG050763.
PhylomeDBi Q02294.

Miscellaneous databases

PROi Q02294.

Gene expression databases

Genevestigatori Q02294.

Family and domain databases

Gene3Di 1.20.120.350. 4 hits.
InterProi IPR027359. Channel_four-helix_dom.
IPR002048. EF_hand_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR005447. VDCC_N_a1su.
IPR002077. VDCCAlpha1.
[Graphical view ]
Pfami PF08763. Ca_chan_IQ. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view ]
PRINTSi PR00167. CACHANNEL.
PR01631. NVDCCALPHA1.
SMARTi SM01062. Ca_chan_IQ. 1 hit.
[Graphical view ]
PROSITEi PS50222. EF_HAND_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the alpha-1 subunit of an omega-conotoxin-sensitive calcium channel."
    Dubel S.J., Starr T.V.B., Hell J.W., Ahlijanian M.K., Enyeart J.J., Catterall W.A., Snutch T.P.
    Proc. Natl. Acad. Sci. U.S.A. 89:5058-5062(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1516-1679 (CLONE RBB-10).
  3. "Differential phosphorylation of two size forms of the N-type calcium channel alpha 1 subunit which have different COOH termini."
    Hell J.W., Appleyard S.M., Yokoyama C.T., Warner C., Catterall W.A.
    J. Biol. Chem. 269:7390-7396(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  4. "Calcium channel beta-subunit binds to a conserved motif in the I-II cytoplasmic linker of the alpha 1-subunit."
    Pragnell M., de Waard M., Mori Y., Tanabe T., Snutch T.P., Campbell K.P.
    Nature 368:67-70(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: BETA-SUBUNIT BINDING DOMAIN.

Entry informationi

Entry nameiCAC1B_RAT
AccessioniPrimary (citable) accession number: Q02294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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