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Protein

5-hydroxytryptamine receptor 1F

Gene

Htr1f

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.1 Publication

GO - Molecular functioni

  • serotonin binding Source: MGI
  • serotonin receptor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_331048. G alpha (i) signalling events.
REACT_332615. Serotonin receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1F
Short name:
5-HT-1F
Short name:
5-HT1F
Alternative name(s):
5-HT-1E-beta
Serotonin receptor 1F
Gene namesi
Name:Htr1f
Synonyms:5ht1f, Htr1eb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:99842. Htr1f.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2929ExtracellularBy similarityAdd
BLAST
Transmembranei30 – 5021Helical; Name=1By similarityAdd
BLAST
Topological domaini51 – 6010CytoplasmicBy similarity
Transmembranei61 – 8323Helical; Name=2By similarityAdd
BLAST
Topological domaini84 – 9714ExtracellularBy similarityAdd
BLAST
Transmembranei98 – 11821Helical; Name=3By similarityAdd
BLAST
Topological domaini119 – 14022CytoplasmicBy similarityAdd
BLAST
Transmembranei141 – 16121Helical; Name=4By similarityAdd
BLAST
Topological domaini162 – 17817ExtracellularBy similarityAdd
BLAST
Transmembranei179 – 20123Helical; Name=5By similarityAdd
BLAST
Topological domaini202 – 29089CytoplasmicBy similarityAdd
BLAST
Transmembranei291 – 31121Helical; Name=6By similarityAdd
BLAST
Topological domaini312 – 32918ExtracellularBy similarityAdd
BLAST
Transmembranei330 – 35021Helical; Name=7By similarityAdd
BLAST
Topological domaini351 – 36616CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3663665-hydroxytryptamine receptor 1FPRO_0000068938Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
Glycosylationi10 – 101N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi96 ↔ 172PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ02284.

PTM databases

PhosphoSiteiQ02284.

Expressioni

Tissue specificityi

Detected in hippocampus.1 Publication

Gene expression databases

BgeeiQ02284.
CleanExiMM_HTR1F.
ExpressionAtlasiQ02284. baseline and differential.
GenevisibleiQ02284. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063136.

Structurei

3D structure databases

ProteinModelPortaliQ02284.
SMRiQ02284. Positions 20-363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 10810Agonist bindingBy similarity
Regioni306 – 3105Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi120 – 1223DRY motif; important for ligand-induced conformation changesBy similarity
Motifi343 – 3475NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249628.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiQ02284.
KOiK04153.
OMAiVVNVCEK.
OrthoDBiEOG7NCV3Q.
PhylomeDBiQ02284.
TreeFamiTF316350.

Family and domain databases

InterProiIPR000450. 5HT1F_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF34. PTHR24247:SF34. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00515. 5HT1FRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFLNASDQN LTSEELLNRM PSKILVSLTL SGLALMTTTI NSLVIAAIIV
60 70 80 90 100
TRKLHHPANY LICSLAVTDF LVAVLVMPFS IVYIVRESWI MGQVLCDIWL
110 120 130 140 150
SVDIICCTCS ILHLSAIALD RYRAITDAVE YARKRTPRHA GIMITIVWVI
160 170 180 190 200
SVFISMPPLF WRHQGTSRDD ECVIKHDHIV STIYSTFGAF YIPLVLILIL
210 220 230 240 250
YYKIYRAART LYHKRQASRM IKEELNGQVF LESGEKSIKL VSTSYMLEKS
260 270 280 290 300
LSDPSTDFDR IHSTVKSPRS ELKHEKSWRR QKISGTRERK AATTLGLILG
310 320 330 340 350
AFVICWLPFF VKELVVNVCE KCKISEEMSN FLAWLGYLNS LINPLIYTIF
360
NEDFKKAFQK LVRCRY
Length:366
Mass (Da):41,977
Last modified:April 1, 1993 - v1
Checksum:i69EC1CD6F22BD93F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14224 mRNA. Translation: CAA78593.1.
CCDSiCCDS28267.1.
PIRiS26048.
RefSeqiNP_032336.1. NM_008310.3.
UniGeneiMm.5040.

Genome annotation databases

EnsembliENSMUST00000063076; ENSMUSP00000063136; ENSMUSG00000050783.
GeneIDi15557.
KEGGimmu:15557.
UCSCiuc007zqg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14224 mRNA. Translation: CAA78593.1.
CCDSiCCDS28267.1.
PIRiS26048.
RefSeqiNP_032336.1. NM_008310.3.
UniGeneiMm.5040.

3D structure databases

ProteinModelPortaliQ02284.
SMRiQ02284. Positions 20-363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063136.

Chemistry

BindingDBiQ02284.
GuidetoPHARMACOLOGYi5.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ02284.

Proteomic databases

PRIDEiQ02284.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063076; ENSMUSP00000063136; ENSMUSG00000050783.
GeneIDi15557.
KEGGimmu:15557.
UCSCiuc007zqg.2. mouse.

Organism-specific databases

CTDi3355.
MGIiMGI:99842. Htr1f.

Phylogenomic databases

eggNOGiNOG249628.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiQ02284.
KOiK04153.
OMAiVVNVCEK.
OrthoDBiEOG7NCV3Q.
PhylomeDBiQ02284.
TreeFamiTF316350.

Enzyme and pathway databases

ReactomeiREACT_331048. G alpha (i) signalling events.
REACT_332615. Serotonin receptors.

Miscellaneous databases

NextBioi288496.
PROiQ02284.
SOURCEiSearch...

Gene expression databases

BgeeiQ02284.
CleanExiMM_HTR1F.
ExpressionAtlasiQ02284. baseline and differential.
GenevisibleiQ02284. MM.

Family and domain databases

InterProiIPR000450. 5HT1F_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF34. PTHR24247:SF34. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00515. 5HT1FRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of a mouse '5HT1E-like' serotonin receptor expressed predominantly in hippocampus."
    Amlaiky N., Ramboz S., Boschert U., Plassat J.-L., Hen R.
    J. Biol. Chem. 267:19761-19764(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry namei5HT1F_MOUSE
AccessioniPrimary (citable) accession number: Q02284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 24, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.