Q02254 (NDK1_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase 1
EC=2.7.4.6

Alternative name(s):
Nucleoside diphosphate kinase I
Short name=NDK I
Short name=NDP kinase I
Short name=NDPK I

Gene names

Name:

NDPK1

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	Nucleoside diphosphate kinase 1		PRO_0000137145
Sites
	Active site	115	1	Pros-phosphohistidine intermediate By similarity
	Binding site	9	1	ATP By similarity
	Binding site	57	1	ATP By similarity
	Binding site	85	1	ATP By similarity
	Binding site	91	1	ATP By similarity
	Binding site	102	1	ATP By similarity
	Binding site	112	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02254 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 0100E17AFD272CFB	FASTA	148	16,290
	10 20 30 40 50 60 MEQTFIMIKP DGVQRGLVGE IISRFEKKGF SLKALKFVNV DRPFAEKHYA DLSAKPFFNG 70 80 90 100 110 120 LVEYIVSGPV VAMVWEGKGV VATGRKLIGA TNPLASEPGT IRGDFAIDIG RNVIHGSDAV 130 140 DSATKEIALW FPDGVVHWQS SLHSWIYE

References

[1]	"The amino acid sequence of nucleoside diphosphate kinase I from spinach leaves, as deduced from the cDNA sequence." Nomura T., Yatsunami K., Honda A., Sugimoto Y., Fukui T., Zhang J., Yamamoto J., Ichikawa A. Arch. Biochem. Biophys. 297:42-45(1992) [PubMed: 1322113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10659 mRNA. Translation: BAA01510.1.
PIR	S24165.
3D structure databases
ProteinModelPortal	Q02254.
SMR	Q02254. Positions 1-148.
ModBase	Search...
Proteomic databases
PRIDE	Q02254.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001564. Nucleoside_diP_kinase. IPR023005. Nucleoside_diP_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK1_SPIOL

Accession

Primary (citable) accession number: Q02254

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 31, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program