Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial precursor

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Q02253 (MMSA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
Short name=MMSDH
Short name=Malonate-semialdehyde dehydrogenase [acylating]
EC=1.2.1.18
EC=1.2.1.27

Alternative name(s):
Aldehyde dehydrogenase family 6 member A1

Gene names

Name:	Aldh6a1
Synonyms:	Mmsdh

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
Catalytic activity	2-methyl-3-oxopropanoate + CoA + H₂O + NAD⁺ = propanoyl-CoA + HCO₃^- + NADH. 3-oxopropanoate + CoA + NAD(P)⁺ = acetyl-CoA + CO₂ + NAD(P)H.
Subunit structure	Homotetramer.
Subcellular location	Mitochondrion.
Tissue specificity	Expressed in the head and flagellum of epididymal sperm but not in testicular sperm (at protein level). Kidney > liver > heart > muscle > brain. Ref.5
Sequence similarities	Belongs to the aldehyde dehydrogenase family.
Mass spectrometry	Molecular mass is 57770.57 Da from positions 1 - 535. Determined by MALDI. Ref.5

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	beta-alanine catabolic process Inferred from direct assay Ref.2. Source: RGD thymine catabolic process Inferred from direct assay Ref.2. Source: RGD valine catabolic process Inferred from direct assay Ref.2. Source: RGD
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	fatty-acyl-CoA binding Inferred from sequence or structural similarity. Source: UniProtKB malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from direct assay Ref.2. Source: UniProtKB methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from direct assay Ref.2. Source: UniProtKB thiolester hydrolase activity Inferred from direct assay PubMed 1540637. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 32

Mitochondrion Ref.1 Ref.2 Ref.3

Chain

33 – 535

503

Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial

PRO_0000007190

Regions

Nucleotide binding

209 – 213

NAD Potential

Nucleotide binding

261 – 266

NAD Potential

Sites

Active site

317

Nucleophile By similarity

Binding site

417

NAD Potential

Amino acid modifications

Modified residue

N6-acetyllysine By similarity

Modified residue

117

N6-acetyllysine By similarity

Modified residue

331

N6-acetyllysine By similarity

Experimental info

Sequence conflict

D → N AA sequence Ref.1

Sequence conflict

E → Q AA sequence Ref.1

Sequence conflict

166

D → N AA sequence Ref.1

Sequence conflict

168

D → N AA sequence Ref.1

Sequence conflict

184

P → T AA sequence Ref.1

Sequence conflict

190

M → G AA sequence Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q02253 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: D914CE0311AA466A
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FASTA

535

57,808

        10         20         30         40         50         60 
MAAAVAAAAA VRSRILQVSS KVNSTWYPAS SFSSSSVPTV KLFIDGKFVE SKSDKWIDIH 

        70         80         90        100        110        120 
NPATNEVVGR VPQSTKAEME AAVAACKRAF PAWADTSILS RQQVLLRYQQ LIKENLKEIA 

       130        140        150        160        170        180 
RLITLEQGKT LADAEGDVFR GLQVVEHACS VTSLMLGETM PSITKDMDLY SYRLPLGVCA 

       190        200        210        220        230        240 
GIAPFNFPAM IPLWMFPMAM VCGNTFLMKP SERVPGATML LAKLLQDSGA PDGTLNIIHG 

       250        260        270        280        290        300 
QHEAVNFICD HPDIKAISFV GSNQAGEYIF ERGSRNGKRV QANMGAKNHG VVMPDANKEN 

       310        320        330        340        350        360 
TLNQLVGAAF GAAGQRCMAL STAVLVGEAK KWLPELVERA KNLRVNAGDQ PGADLGPLIT 

       370        380        390        400        410        420 
PQAKERVCNL IDSGAKEGAS ILLDGRKIKV KGYENGNFVG PTIISNVKPS MTCYKEEIFG 

       430        440        450        460        470        480 
PVLVVLETET LDEAIKIVND NPYGNGTAIF TTNGAIARKY AHMVDVGQVG VNVPIPVPLP 

       490        500        510        520        530 
MFSFTGSRSS FRGDTNFYGK QGIQFYTQLK TITSQWKEED ATLSSPAVVM PTMGR

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References

[1]	"CoA-dependent methylmalonate-semialdehyde dehydrogenase, a unique member of the aldehyde dehydrogenase superfamily. cDNA cloning, evolutionary relationships, and tissue distribution." Kedishvili N.Y., Popov K.M., Rougraff P.M., Zhao Y., Crabb D.W., Harris R.A. J. Biol. Chem. 267:19724-19729(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-50 AND 166-190. Tissue: Liver.
[2]	"Purification and characterization of methylmalonate-semialdehyde dehydrogenase from rat liver. Identity to malonate-semialdehyde dehydrogenase." Goodwin G.W., Rougraff P.M., Davis E.J., Harris R.A. J. Biol. Chem. 264:14965-14971(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 33-50, CHARACTERIZATION.
[3]	"The effect of ligand binding on the proteolytic pattern of methylmalonate semialdehyde dehydrogenase." Kedishvili N.Y., Popov K.M., Harris R.A. Arch. Biochem. Biophys. 290:21-26(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
[4]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 56-70, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.
[5]	"Differential proteomics leads to identification of domain specific epididymal sperm proteins." Suryawanshi A.R., Khan S.A., Gajbhiye R.K., Gurav M.Y., Khole V.V. J. Androl. 32:240-259(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, MASS SPECTROMETRY. Strain: Holtzman. Tissue: Sperm.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M93401 mRNA. Translation: AAA41638.1.
IPI	IPI00205018.
PIR	A44097.
RefSeq	NP_112319.2. NM_031057.2.
UniGene	Rn.2098.
3D structure databases
ProteinModelPortal	Q02253.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q02253. 1 interaction.
STRING	10116.ENSRNOP00000015545.
Proteomic databases
PaxDb	Q02253.
PRIDE	Q02253.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81708.
KEGG	rno:81708.
UCSC	RGD:621556. rat.
Organism-specific databases
CTD	4329.
RGD	621556. Aldh6a1.
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HOG000271507.
HOVERGEN	HBG105023.
InParanoid	Q02253.
KO	K00140.
OrthoDB	EOG4HHP25.
Enzyme and pathway databases
SABIO-RK	Q02253.
Gene expression databases
Genevestigator	Q02253.
GermOnline	ENSRNOG00000011419. Rattus norvegicus.
Family and domain databases
Gene3D	3.40.309.10. 1 hit. 3.40.605.10. 1 hit.
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR010061. MeMal-semiAld_DH. [Graphical view]
PANTHER	PTHR11699:SF27. PTHR11699:SF27. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	615332.

Entry information

Entry name

MMSA_RAT

Accession

Primary (citable) accession number: Q02253

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents