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Q02253

- MMSA_RAT

UniProt

Q02253 - MMSA_RAT

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Protein
Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
Gene
Aldh6a1, Mmsdh
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.

Catalytic activityi

2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH.
3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei317 – 3171Nucleophile By similarity
Binding sitei417 – 4171NAD Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi209 – 2135NAD Reviewed prediction
Nucleotide bindingi261 – 2666NAD Reviewed prediction

GO - Molecular functioni

  1. fatty-acyl-CoA binding Source: UniProtKB
  2. malonate-semialdehyde dehydrogenase (acetylating) activity Source: UniProtKB
  3. methylmalonate-semialdehyde dehydrogenase (acylating) activity Source: UniProtKB
  4. thiolester hydrolase activity Source: RGD

GO - Biological processi

  1. beta-alanine catabolic process Source: RGD
  2. thymine catabolic process Source: RGD
  3. thymine metabolic process Source: UniProtKB
  4. valine catabolic process Source: RGD
  5. valine metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKQ02253.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (EC:1.2.1.18, EC:1.2.1.27)
Short name:
MMSDH
Short name:
Malonate-semialdehyde dehydrogenase [acylating]
Alternative name(s):
Aldehyde dehydrogenase family 6 member A1
Gene namesi
Name:Aldh6a1
Synonyms:Mmsdh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621556. Aldh6a1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232Mitochondrion3 Publications
Add
BLAST
Chaini33 – 535503Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
PRO_0000007190Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei47 – 471N6-acetyllysine; alternate By similarity
Modified residuei47 – 471N6-succinyllysine; alternate By similarity
Modified residuei52 – 521N6-acetyllysine; alternate By similarity
Modified residuei52 – 521N6-succinyllysine; alternate By similarity
Modified residuei55 – 551N6-acetyllysine; alternate By similarity
Modified residuei55 – 551N6-succinyllysine; alternate By similarity
Modified residuei76 – 761N6-acetyllysine; alternate By similarity
Modified residuei76 – 761N6-succinyllysine; alternate By similarity
Modified residuei87 – 871N6-acetyllysine By similarity
Modified residuei117 – 1171N6-acetyllysine; alternate By similarity
Modified residuei117 – 1171N6-succinyllysine; alternate By similarity
Modified residuei129 – 1291N6-acetyllysine; alternate By similarity
Modified residuei129 – 1291N6-succinyllysine; alternate By similarity
Modified residuei298 – 2981N6-acetyllysine By similarity
Modified residuei330 – 3301N6-acetyllysine By similarity
Modified residuei331 – 3311N6-acetyllysine By similarity
Modified residuei364 – 3641N6-acetyllysine; alternate By similarity
Modified residuei364 – 3641N6-succinyllysine; alternate By similarity
Modified residuei376 – 3761N6-acetyllysine; alternate By similarity
Modified residuei376 – 3761N6-succinyllysine; alternate By similarity
Modified residuei391 – 3911N6-succinyllysine By similarity
Modified residuei500 – 5001N6-acetyllysine By similarity
Modified residuei517 – 5171N6-succinyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ02253.
PRIDEiQ02253.

Expressioni

Tissue specificityi

Expressed in the head and flagellum of epididymal sperm but not in testicular sperm (at protein level). Kidney > liver > heart > muscle > brain.1 Publication

Gene expression databases

GenevestigatoriQ02253.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

IntActiQ02253. 1 interaction.
STRINGi10116.ENSRNOP00000015545.

Structurei

3D structure databases

ProteinModelPortaliQ02253.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271507.
HOVERGENiHBG105023.
InParanoidiQ02253.
KOiK00140.
PhylomeDBiQ02253.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010061. MeMal-semiAld_DH.
[Graphical view]
PANTHERiPTHR11699:SF27. PTHR11699:SF27. 1 hit.
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01722. MMSDH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02253-1 [UniParc]FASTAAdd to Basket

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MAAAVAAAAA VRSRILQVSS KVNSTWYPAS SFSSSSVPTV KLFIDGKFVE    50
SKSDKWIDIH NPATNEVVGR VPQSTKAEME AAVAACKRAF PAWADTSILS 100
RQQVLLRYQQ LIKENLKEIA RLITLEQGKT LADAEGDVFR GLQVVEHACS 150
VTSLMLGETM PSITKDMDLY SYRLPLGVCA GIAPFNFPAM IPLWMFPMAM 200
VCGNTFLMKP SERVPGATML LAKLLQDSGA PDGTLNIIHG QHEAVNFICD 250
HPDIKAISFV GSNQAGEYIF ERGSRNGKRV QANMGAKNHG VVMPDANKEN 300
TLNQLVGAAF GAAGQRCMAL STAVLVGEAK KWLPELVERA KNLRVNAGDQ 350
PGADLGPLIT PQAKERVCNL IDSGAKEGAS ILLDGRKIKV KGYENGNFVG 400
PTIISNVKPS MTCYKEEIFG PVLVVLETET LDEAIKIVND NPYGNGTAIF 450
TTNGAIARKY AHMVDVGQVG VNVPIPVPLP MFSFTGSRSS FRGDTNFYGK 500
QGIQFYTQLK TITSQWKEED ATLSSPAVVM PTMGR 535
Length:535
Mass (Da):57,808
Last modified:July 1, 1993 - v1
Checksum:iD914CE0311AA466A
GO

Mass spectrometryi

Molecular mass is 57770.57 Da from positions 1 - 535. Determined by MALDI. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451D → N AA sequence 1 Publication
Sequence conflicti50 – 501E → Q AA sequence 1 Publication
Sequence conflicti166 – 1661D → N AA sequence 1 Publication
Sequence conflicti168 – 1681D → N AA sequence 1 Publication
Sequence conflicti184 – 1841P → T AA sequence 1 Publication
Sequence conflicti190 – 1901M → G AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93401 mRNA. Translation: AAA41638.1.
PIRiA44097.
RefSeqiNP_112319.2. NM_031057.2.
UniGeneiRn.2098.

Genome annotation databases

GeneIDi81708.
KEGGirno:81708.
UCSCiRGD:621556. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93401 mRNA. Translation: AAA41638.1 .
PIRi A44097.
RefSeqi NP_112319.2. NM_031057.2.
UniGenei Rn.2098.

3D structure databases

ProteinModelPortali Q02253.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q02253. 1 interaction.
STRINGi 10116.ENSRNOP00000015545.

Proteomic databases

PaxDbi Q02253.
PRIDEi Q02253.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 81708.
KEGGi rno:81708.
UCSCi RGD:621556. rat.

Organism-specific databases

CTDi 4329.
RGDi 621556. Aldh6a1.

Phylogenomic databases

eggNOGi COG1012.
HOGENOMi HOG000271507.
HOVERGENi HBG105023.
InParanoidi Q02253.
KOi K00140.
PhylomeDBi Q02253.

Enzyme and pathway databases

SABIO-RK Q02253.

Miscellaneous databases

NextBioi 615332.
PROi Q02253.

Gene expression databases

Genevestigatori Q02253.

Family and domain databases

Gene3Di 3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010061. MeMal-semiAld_DH.
[Graphical view ]
PANTHERi PTHR11699:SF27. PTHR11699:SF27. 1 hit.
Pfami PF00171. Aldedh. 1 hit.
[Graphical view ]
SUPFAMi SSF53720. SSF53720. 1 hit.
TIGRFAMsi TIGR01722. MMSDH. 1 hit.
PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "CoA-dependent methylmalonate-semialdehyde dehydrogenase, a unique member of the aldehyde dehydrogenase superfamily. cDNA cloning, evolutionary relationships, and tissue distribution."
    Kedishvili N.Y., Popov K.M., Rougraff P.M., Zhao Y., Crabb D.W., Harris R.A.
    J. Biol. Chem. 267:19724-19729(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-50 AND 166-190.
    Tissue: Liver.
  2. "Purification and characterization of methylmalonate-semialdehyde dehydrogenase from rat liver. Identity to malonate-semialdehyde dehydrogenase."
    Goodwin G.W., Rougraff P.M., Davis E.J., Harris R.A.
    J. Biol. Chem. 264:14965-14971(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 33-50, CHARACTERIZATION.
  3. "The effect of ligand binding on the proteolytic pattern of methylmalonate semialdehyde dehydrogenase."
    Kedishvili N.Y., Popov K.M., Harris R.A.
    Arch. Biochem. Biophys. 290:21-26(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
  4. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 56-70, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.
  5. "Differential proteomics leads to identification of domain specific epididymal sperm proteins."
    Suryawanshi A.R., Khan S.A., Gajbhiye R.K., Gurav M.Y., Khole V.V.
    J. Androl. 32:240-259(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, MASS SPECTROMETRY.
    Strain: Holtzman.
    Tissue: Sperm.

Entry informationi

Entry nameiMMSA_RAT
AccessioniPrimary (citable) accession number: Q02253
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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