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Protein

Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial

Gene

Aldh6a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.

Catalytic activityi

2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH.
3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei317NucleophilePROSITE-ProRule annotation1
Binding sitei417NADSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi209 – 213NADSequence analysis5
Nucleotide bindingi261 – 266NADSequence analysis6

GO - Molecular functioni

  • aldehyde dehydrogenase (NAD) activity Source: GO_Central
  • fatty-acyl-CoA binding Source: UniProtKB
  • malonate-semialdehyde dehydrogenase (acetylating) activity Source: UniProtKB
  • methylmalonate-semialdehyde dehydrogenase (acylating) activity Source: UniProtKB
  • thiolester hydrolase activity Source: RGD

GO - Biological processi

  • beta-alanine catabolic process Source: RGD
  • thymine catabolic process Source: RGD
  • thymine metabolic process Source: UniProtKB
  • valine catabolic process Source: RGD
  • valine metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKQ02253.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (EC:1.2.1.18, EC:1.2.1.27)
Short name:
MMSDH
Short name:
Malonate-semialdehyde dehydrogenase [acylating]
Alternative name(s):
Aldehyde dehydrogenase family 6 member A1
Gene namesi
Name:Aldh6a1
Synonyms:Mmsdh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621556. Aldh6a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32Mitochondrion3 PublicationsAdd BLAST32
ChainiPRO_000000719033 – 535Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrialAdd BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei47N6-acetyllysine; alternateBy similarity1
Modified residuei47N6-succinyllysine; alternateBy similarity1
Modified residuei52N6-acetyllysine; alternateBy similarity1
Modified residuei52N6-succinyllysine; alternateBy similarity1
Modified residuei55N6-acetyllysine; alternateBy similarity1
Modified residuei55N6-succinyllysine; alternateBy similarity1
Modified residuei76N6-acetyllysine; alternateBy similarity1
Modified residuei76N6-succinyllysine; alternateBy similarity1
Modified residuei87N6-acetyllysineBy similarity1
Modified residuei117N6-acetyllysine; alternateBy similarity1
Modified residuei117N6-succinyllysine; alternateBy similarity1
Modified residuei129N6-acetyllysine; alternateBy similarity1
Modified residuei129N6-succinyllysine; alternateBy similarity1
Modified residuei262PhosphoserineBy similarity1
Modified residuei298N6-acetyllysineBy similarity1
Modified residuei330N6-acetyllysineBy similarity1
Modified residuei331N6-acetyllysineBy similarity1
Modified residuei364N6-acetyllysine; alternateBy similarity1
Modified residuei364N6-succinyllysine; alternateBy similarity1
Modified residuei376N6-acetyllysine; alternateBy similarity1
Modified residuei376N6-succinyllysine; alternateBy similarity1
Modified residuei380PhosphoserineBy similarity1
Modified residuei391N6-succinyllysineBy similarity1
Modified residuei500N6-acetyllysineBy similarity1
Modified residuei517N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ02253.
PRIDEiQ02253.

PTM databases

iPTMnetiQ02253.
PhosphoSitePlusiQ02253.

Expressioni

Tissue specificityi

Expressed in the head and flagellum of epididymal sperm but not in testicular sperm (at protein level). Kidney > liver > heart > muscle > brain.1 Publication

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

IntActiQ02253. 1 interactor.
STRINGi10116.ENSRNOP00000015545.

Structurei

3D structure databases

ProteinModelPortaliQ02253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2449. Eukaryota.
ENOG410XNP1. LUCA.
HOGENOMiHOG000271507.
HOVERGENiHBG105023.
InParanoidiQ02253.
KOiK00140.
PhylomeDBiQ02253.

Family and domain databases

CDDicd07085. ALDH_F6_MMSDH. 1 hit.
Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010061. MeMal-semiAld_DH.
[Graphical view]
PANTHERiPTHR11699:SF183. PTHR11699:SF183. 1 hit.
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01722. MMSDH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02253-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAVAAAAA VRSRILQVSS KVNSTWYPAS SFSSSSVPTV KLFIDGKFVE
60 70 80 90 100
SKSDKWIDIH NPATNEVVGR VPQSTKAEME AAVAACKRAF PAWADTSILS
110 120 130 140 150
RQQVLLRYQQ LIKENLKEIA RLITLEQGKT LADAEGDVFR GLQVVEHACS
160 170 180 190 200
VTSLMLGETM PSITKDMDLY SYRLPLGVCA GIAPFNFPAM IPLWMFPMAM
210 220 230 240 250
VCGNTFLMKP SERVPGATML LAKLLQDSGA PDGTLNIIHG QHEAVNFICD
260 270 280 290 300
HPDIKAISFV GSNQAGEYIF ERGSRNGKRV QANMGAKNHG VVMPDANKEN
310 320 330 340 350
TLNQLVGAAF GAAGQRCMAL STAVLVGEAK KWLPELVERA KNLRVNAGDQ
360 370 380 390 400
PGADLGPLIT PQAKERVCNL IDSGAKEGAS ILLDGRKIKV KGYENGNFVG
410 420 430 440 450
PTIISNVKPS MTCYKEEIFG PVLVVLETET LDEAIKIVND NPYGNGTAIF
460 470 480 490 500
TTNGAIARKY AHMVDVGQVG VNVPIPVPLP MFSFTGSRSS FRGDTNFYGK
510 520 530
QGIQFYTQLK TITSQWKEED ATLSSPAVVM PTMGR
Length:535
Mass (Da):57,808
Last modified:July 1, 1993 - v1
Checksum:iD914CE0311AA466A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45D → N AA sequence (PubMed:1527093).Curated1
Sequence conflicti50E → Q AA sequence (PubMed:1527093).Curated1
Sequence conflicti166D → N AA sequence (PubMed:1527093).Curated1
Sequence conflicti168D → N AA sequence (PubMed:1527093).Curated1
Sequence conflicti184P → T AA sequence (PubMed:1527093).Curated1
Sequence conflicti190M → G AA sequence (PubMed:1527093).Curated1

Mass spectrometryi

Molecular mass is 57770.57 Da from positions 1 - 535. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93401 mRNA. Translation: AAA41638.1.
PIRiA44097.
RefSeqiNP_112319.2. NM_031057.2.
UniGeneiRn.2098.

Genome annotation databases

GeneIDi81708.
KEGGirno:81708.
UCSCiRGD:621556. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93401 mRNA. Translation: AAA41638.1.
PIRiA44097.
RefSeqiNP_112319.2. NM_031057.2.
UniGeneiRn.2098.

3D structure databases

ProteinModelPortaliQ02253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ02253. 1 interactor.
STRINGi10116.ENSRNOP00000015545.

PTM databases

iPTMnetiQ02253.
PhosphoSitePlusiQ02253.

Proteomic databases

PaxDbiQ02253.
PRIDEiQ02253.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi81708.
KEGGirno:81708.
UCSCiRGD:621556. rat.

Organism-specific databases

CTDi4329.
RGDi621556. Aldh6a1.

Phylogenomic databases

eggNOGiKOG2449. Eukaryota.
ENOG410XNP1. LUCA.
HOGENOMiHOG000271507.
HOVERGENiHBG105023.
InParanoidiQ02253.
KOiK00140.
PhylomeDBiQ02253.

Enzyme and pathway databases

SABIO-RKQ02253.

Miscellaneous databases

PROiQ02253.

Family and domain databases

CDDicd07085. ALDH_F6_MMSDH. 1 hit.
Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010061. MeMal-semiAld_DH.
[Graphical view]
PANTHERiPTHR11699:SF183. PTHR11699:SF183. 1 hit.
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01722. MMSDH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMSA_RAT
AccessioniPrimary (citable) accession number: Q02253
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.