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Q02253

- MMSA_RAT

UniProt

Q02253 - MMSA_RAT

Protein

Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial

Gene

Aldh6a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.

    Catalytic activityi

    2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH.
    3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei317 – 3171NucleophilePROSITE-ProRule annotation
    Binding sitei417 – 4171NADSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi209 – 2135NADSequence Analysis
    Nucleotide bindingi261 – 2666NADSequence Analysis

    GO - Molecular functioni

    1. fatty-acyl-CoA binding Source: UniProtKB
    2. malonate-semialdehyde dehydrogenase (acetylating) activity Source: UniProtKB
    3. methylmalonate-semialdehyde dehydrogenase (acylating) activity Source: UniProtKB
    4. thiolester hydrolase activity Source: RGD

    GO - Biological processi

    1. beta-alanine catabolic process Source: RGD
    2. thymine catabolic process Source: RGD
    3. thymine metabolic process Source: UniProtKB
    4. valine catabolic process Source: RGD
    5. valine metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    SABIO-RKQ02253.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (EC:1.2.1.18, EC:1.2.1.27)
    Short name:
    MMSDH
    Short name:
    Malonate-semialdehyde dehydrogenase [acylating]
    Alternative name(s):
    Aldehyde dehydrogenase family 6 member A1
    Gene namesi
    Name:Aldh6a1
    Synonyms:Mmsdh
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621556. Aldh6a1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3232Mitochondrion3 PublicationsAdd
    BLAST
    Chaini33 – 535503Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrialPRO_0000007190Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei47 – 471N6-acetyllysine; alternateBy similarity
    Modified residuei47 – 471N6-succinyllysine; alternateBy similarity
    Modified residuei52 – 521N6-acetyllysine; alternateBy similarity
    Modified residuei52 – 521N6-succinyllysine; alternateBy similarity
    Modified residuei55 – 551N6-acetyllysine; alternateBy similarity
    Modified residuei55 – 551N6-succinyllysine; alternateBy similarity
    Modified residuei76 – 761N6-acetyllysine; alternateBy similarity
    Modified residuei76 – 761N6-succinyllysine; alternateBy similarity
    Modified residuei87 – 871N6-acetyllysineBy similarity
    Modified residuei117 – 1171N6-acetyllysine; alternateBy similarity
    Modified residuei117 – 1171N6-succinyllysine; alternateBy similarity
    Modified residuei129 – 1291N6-acetyllysine; alternateBy similarity
    Modified residuei129 – 1291N6-succinyllysine; alternateBy similarity
    Modified residuei298 – 2981N6-acetyllysineBy similarity
    Modified residuei330 – 3301N6-acetyllysineBy similarity
    Modified residuei331 – 3311N6-acetyllysineBy similarity
    Modified residuei364 – 3641N6-acetyllysine; alternateBy similarity
    Modified residuei364 – 3641N6-succinyllysine; alternateBy similarity
    Modified residuei376 – 3761N6-acetyllysine; alternateBy similarity
    Modified residuei376 – 3761N6-succinyllysine; alternateBy similarity
    Modified residuei391 – 3911N6-succinyllysineBy similarity
    Modified residuei500 – 5001N6-acetyllysineBy similarity
    Modified residuei517 – 5171N6-succinyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ02253.
    PRIDEiQ02253.

    Expressioni

    Tissue specificityi

    Expressed in the head and flagellum of epididymal sperm but not in testicular sperm (at protein level). Kidney > liver > heart > muscle > brain.1 Publication

    Gene expression databases

    GenevestigatoriQ02253.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    IntActiQ02253. 1 interaction.
    STRINGi10116.ENSRNOP00000015545.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02253.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1012.
    HOGENOMiHOG000271507.
    HOVERGENiHBG105023.
    InParanoidiQ02253.
    KOiK00140.
    PhylomeDBiQ02253.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR010061. MeMal-semiAld_DH.
    [Graphical view]
    PANTHERiPTHR11699:SF27. PTHR11699:SF27. 1 hit.
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    SUPFAMiSSF53720. SSF53720. 1 hit.
    TIGRFAMsiTIGR01722. MMSDH. 1 hit.
    PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q02253-1 [UniParc]FASTAAdd to Basket

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    MAAAVAAAAA VRSRILQVSS KVNSTWYPAS SFSSSSVPTV KLFIDGKFVE    50
    SKSDKWIDIH NPATNEVVGR VPQSTKAEME AAVAACKRAF PAWADTSILS 100
    RQQVLLRYQQ LIKENLKEIA RLITLEQGKT LADAEGDVFR GLQVVEHACS 150
    VTSLMLGETM PSITKDMDLY SYRLPLGVCA GIAPFNFPAM IPLWMFPMAM 200
    VCGNTFLMKP SERVPGATML LAKLLQDSGA PDGTLNIIHG QHEAVNFICD 250
    HPDIKAISFV GSNQAGEYIF ERGSRNGKRV QANMGAKNHG VVMPDANKEN 300
    TLNQLVGAAF GAAGQRCMAL STAVLVGEAK KWLPELVERA KNLRVNAGDQ 350
    PGADLGPLIT PQAKERVCNL IDSGAKEGAS ILLDGRKIKV KGYENGNFVG 400
    PTIISNVKPS MTCYKEEIFG PVLVVLETET LDEAIKIVND NPYGNGTAIF 450
    TTNGAIARKY AHMVDVGQVG VNVPIPVPLP MFSFTGSRSS FRGDTNFYGK 500
    QGIQFYTQLK TITSQWKEED ATLSSPAVVM PTMGR 535
    Length:535
    Mass (Da):57,808
    Last modified:July 1, 1993 - v1
    Checksum:iD914CE0311AA466A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 451D → N AA sequence (PubMed:1527093)Curated
    Sequence conflicti50 – 501E → Q AA sequence (PubMed:1527093)Curated
    Sequence conflicti166 – 1661D → N AA sequence (PubMed:1527093)Curated
    Sequence conflicti168 – 1681D → N AA sequence (PubMed:1527093)Curated
    Sequence conflicti184 – 1841P → T AA sequence (PubMed:1527093)Curated
    Sequence conflicti190 – 1901M → G AA sequence (PubMed:1527093)Curated

    Mass spectrometryi

    Molecular mass is 57770.57 Da from positions 1 - 535. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93401 mRNA. Translation: AAA41638.1.
    PIRiA44097.
    RefSeqiNP_112319.2. NM_031057.2.
    UniGeneiRn.2098.

    Genome annotation databases

    GeneIDi81708.
    KEGGirno:81708.
    UCSCiRGD:621556. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93401 mRNA. Translation: AAA41638.1 .
    PIRi A44097.
    RefSeqi NP_112319.2. NM_031057.2.
    UniGenei Rn.2098.

    3D structure databases

    ProteinModelPortali Q02253.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q02253. 1 interaction.
    STRINGi 10116.ENSRNOP00000015545.

    Proteomic databases

    PaxDbi Q02253.
    PRIDEi Q02253.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 81708.
    KEGGi rno:81708.
    UCSCi RGD:621556. rat.

    Organism-specific databases

    CTDi 4329.
    RGDi 621556. Aldh6a1.

    Phylogenomic databases

    eggNOGi COG1012.
    HOGENOMi HOG000271507.
    HOVERGENi HBG105023.
    InParanoidi Q02253.
    KOi K00140.
    PhylomeDBi Q02253.

    Enzyme and pathway databases

    SABIO-RK Q02253.

    Miscellaneous databases

    NextBioi 615332.
    PROi Q02253.

    Gene expression databases

    Genevestigatori Q02253.

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR010061. MeMal-semiAld_DH.
    [Graphical view ]
    PANTHERi PTHR11699:SF27. PTHR11699:SF27. 1 hit.
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53720. SSF53720. 1 hit.
    TIGRFAMsi TIGR01722. MMSDH. 1 hit.
    PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CoA-dependent methylmalonate-semialdehyde dehydrogenase, a unique member of the aldehyde dehydrogenase superfamily. cDNA cloning, evolutionary relationships, and tissue distribution."
      Kedishvili N.Y., Popov K.M., Rougraff P.M., Zhao Y., Crabb D.W., Harris R.A.
      J. Biol. Chem. 267:19724-19729(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-50 AND 166-190.
      Tissue: Liver.
    2. "Purification and characterization of methylmalonate-semialdehyde dehydrogenase from rat liver. Identity to malonate-semialdehyde dehydrogenase."
      Goodwin G.W., Rougraff P.M., Davis E.J., Harris R.A.
      J. Biol. Chem. 264:14965-14971(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 33-50, CHARACTERIZATION.
    3. "The effect of ligand binding on the proteolytic pattern of methylmalonate semialdehyde dehydrogenase."
      Kedishvili N.Y., Popov K.M., Harris R.A.
      Arch. Biochem. Biophys. 290:21-26(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
    4. Lubec G., Afjehi-Sadat L.
      Submitted (NOV-2006) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 56-70, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Spinal cord.
    5. "Differential proteomics leads to identification of domain specific epididymal sperm proteins."
      Suryawanshi A.R., Khan S.A., Gajbhiye R.K., Gurav M.Y., Khole V.V.
      J. Androl. 32:240-259(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, MASS SPECTROMETRY.
      Strain: Holtzman.
      Tissue: Sperm.

    Entry informationi

    Entry nameiMMSA_RAT
    AccessioniPrimary (citable) accession number: Q02253
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3