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Q02250 (HEM2_METSC) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-aminolevulinic acid dehydratase

Short name=ALAD
Short name=ALADH
EC=4.2.1.24
Alternative name(s):
Porphobilinogen synthase
Gene names
Name:hemB
Synonyms:alaDH
OrganismMethanothermus sociabilis
Taxonomic identifier2181 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activity

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactor

Binds 1 zinc ion per monomer By similarity.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.

Subunit structure

Homooctamer By similarity.

Sequence similarities

Belongs to the ALADH family.

Ontologies

Keywords
   Biological processHeme biosynthesis
Porphyrin biosynthesis
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionLyase
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

porphobilinogen synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 320320Delta-aminolevulinic acid dehydratase
PRO_0000140524

Sites

Active site1941Schiff-base intermediate with substrate By similarity
Active site2471Schiff-base intermediate with substrate By similarity
Metal binding1191Zinc; catalytic By similarity
Metal binding1211Zinc; catalytic By similarity
Metal binding1291Zinc; catalytic By similarity
Metal binding2321Magnesium By similarity
Binding site2041Substrate 1 By similarity
Binding site2161Substrate 1 By similarity
Binding site2731Substrate 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q02250 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 16F13425105F38D0

FASTA32035,925
        10         20         30         40         50         60 
MKFPYTRMRR LRKNSKIRSL VKETTLSKDD LIYPVFVKEG IKTKEKIPKM PGQYRYSVDE 

        70         80         90        100        110        120 
LIDEAKKLEE KGLKAILVFG IPKKKDKYGS SAYDPNGIVQ KSVKLLKEET DLVVITDVCL 

       130        140        150        160        170        180 
CQYTEHGHCG IVKNKKIVND ETLKYLSKVA LSHAEAGADV VAPSDMMDGR VKAIREELEK 

       190        200        210        220        230        240 
NGFDDVIIMS YSAKYASSFY EPFRSAVYSS PAFGDRSTYQ MDPPNSLEAL REVKLDIDEG 

       250        260        270        280        290        300 
ADIVMVKPAL PYLDIIRLVK DTFGVPTAAY NVSGEYSMIK AAIDANYLSN KVIIETLLSI 

       310        320 
KRAGADLIIT HFAPEIVEEI 

« Hide

References

[1]"Sequence of the 5-aminolevulinic acid dehydratase-encoding gene from the hyperthermophilic methanogen, Methanothermus sociabilis."
Broeckl G., Berchtold M., Behr M., Koenig H.
Gene 119:151-152(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M90083 Genomic DNA. Translation: AAA73226.1.
PIRJC1286.

3D structure databases

ProteinModelPortalQ02250.
SMRQ02250. Positions 6-319.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00251; UER00318.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERPTHR11458. PTHR11458. 1 hit.
PfamPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSPR00144. DALDHYDRTASE.
SMARTSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM2_METSC
AccessionPrimary (citable) accession number: Q02250
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 16, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways