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Q02250

- HEM2_METSC

UniProt

Q02250 - HEM2_METSC

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Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Methanothermus sociabilis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 1 zinc ion per monomer.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi119 – 1191Zinc; catalyticBy similarity
Metal bindingi121 – 1211Zinc; catalyticBy similarity
Metal bindingi129 – 1291Zinc; catalyticBy similarity
Active sitei194 – 1941Schiff-base intermediate with substrateBy similarity
Binding sitei204 – 2041Substrate 1By similarity
Binding sitei216 – 2161Substrate 1By similarity
Metal bindingi232 – 2321MagnesiumBy similarity
Active sitei247 – 2471Schiff-base intermediate with substrateBy similarity
Binding sitei273 – 2731Substrate 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Synonyms:alaDH
OrganismiMethanothermus sociabilis
Taxonomic identifieri2181 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Delta-aminolevulinic acid dehydratasePRO_0000140524Add
BLAST

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ02250.
SMRiQ02250. Positions 6-319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02250-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFPYTRMRR LRKNSKIRSL VKETTLSKDD LIYPVFVKEG IKTKEKIPKM
60 70 80 90 100
PGQYRYSVDE LIDEAKKLEE KGLKAILVFG IPKKKDKYGS SAYDPNGIVQ
110 120 130 140 150
KSVKLLKEET DLVVITDVCL CQYTEHGHCG IVKNKKIVND ETLKYLSKVA
160 170 180 190 200
LSHAEAGADV VAPSDMMDGR VKAIREELEK NGFDDVIIMS YSAKYASSFY
210 220 230 240 250
EPFRSAVYSS PAFGDRSTYQ MDPPNSLEAL REVKLDIDEG ADIVMVKPAL
260 270 280 290 300
PYLDIIRLVK DTFGVPTAAY NVSGEYSMIK AAIDANYLSN KVIIETLLSI
310 320
KRAGADLIIT HFAPEIVEEI
Length:320
Mass (Da):35,925
Last modified:July 1, 1993 - v1
Checksum:i16F13425105F38D0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90083 Genomic DNA. Translation: AAA73226.1.
PIRiJC1286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90083 Genomic DNA. Translation: AAA73226.1 .
PIRi JC1286.

3D structure databases

ProteinModelPortali Q02250.
SMRi Q02250. Positions 6-319.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the 5-aminolevulinic acid dehydratase-encoding gene from the hyperthermophilic methanogen, Methanothermus sociabilis."
    Broeckl G., Berchtold M., Behr M., Koenig H.
    Gene 119:151-152(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiHEM2_METSC
AccessioniPrimary (citable) accession number: Q02250
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3