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Q02250

- HEM2_METSC

UniProt

Q02250 - HEM2_METSC

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Protein

Delta-aminolevulinic acid dehydratase

Gene
hemB, alaDH
Organism
Methanothermus sociabilis
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 1 zinc ion per monomer By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi119 – 1191Zinc; catalytic By similarity
Metal bindingi121 – 1211Zinc; catalytic By similarity
Metal bindingi129 – 1291Zinc; catalytic By similarity
Active sitei194 – 1941Schiff-base intermediate with substrate By similarity
Binding sitei204 – 2041Substrate 1 By similarity
Binding sitei216 – 2161Substrate 1 By similarity
Metal bindingi232 – 2321Magnesium By similarity
Active sitei247 – 2471Schiff-base intermediate with substrate By similarity
Binding sitei273 – 2731Substrate 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Synonyms:alaDH
OrganismiMethanothermus sociabilis
Taxonomic identifieri2181 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Delta-aminolevulinic acid dehydratasePRO_0000140524Add
BLAST

Interactioni

Subunit structurei

Homooctamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ02250.
SMRiQ02250. Positions 6-319.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALADH family.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02250-1 [UniParc]FASTAAdd to Basket

« Hide

MKFPYTRMRR LRKNSKIRSL VKETTLSKDD LIYPVFVKEG IKTKEKIPKM    50
PGQYRYSVDE LIDEAKKLEE KGLKAILVFG IPKKKDKYGS SAYDPNGIVQ 100
KSVKLLKEET DLVVITDVCL CQYTEHGHCG IVKNKKIVND ETLKYLSKVA 150
LSHAEAGADV VAPSDMMDGR VKAIREELEK NGFDDVIIMS YSAKYASSFY 200
EPFRSAVYSS PAFGDRSTYQ MDPPNSLEAL REVKLDIDEG ADIVMVKPAL 250
PYLDIIRLVK DTFGVPTAAY NVSGEYSMIK AAIDANYLSN KVIIETLLSI 300
KRAGADLIIT HFAPEIVEEI 320
Length:320
Mass (Da):35,925
Last modified:July 1, 1993 - v1
Checksum:i16F13425105F38D0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90083 Genomic DNA. Translation: AAA73226.1.
PIRiJC1286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90083 Genomic DNA. Translation: AAA73226.1 .
PIRi JC1286.

3D structure databases

ProteinModelPortali Q02250.
SMRi Q02250. Positions 6-319.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the 5-aminolevulinic acid dehydratase-encoding gene from the hyperthermophilic methanogen, Methanothermus sociabilis."
    Broeckl G., Berchtold M., Behr M., Koenig H.
    Gene 119:151-152(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiHEM2_METSC
AccessioniPrimary (citable) accession number: Q02250
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 16, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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