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Protein

Kinesin-like protein KIF23

Gene

KIF23

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi112 – 119ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • microtubule binding Source: UniProtKB
  • microtubule motor activity Source: GO_Central

GO - Biological processi

  • actomyosin contractile ring assembly Source: InterPro
  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
  • microtubule-based movement Source: GO_Central
  • mitotic cytokinesis Source: UniProtKB
  • mitotic spindle elongation Source: ProtInc
  • mitotic spindle midzone assembly Source: UniProtKB
  • plus-end-directed vesicle transport along microtubule Source: InterPro
  • positive regulation of cytokinesis Source: UniProtKB
  • retrograde vesicle-mediated transport, Golgi to ER Source: Reactome

Keywordsi

Molecular functionMotor protein
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2132295 MHC class II antigen presentation
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-68884 Mitotic Telophase/Cytokinesis
R-HSA-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF23
Alternative name(s):
Kinesin-like protein 5
Mitotic kinesin-like protein 1
Gene namesi
Name:KIF23
Synonyms:KNSL5, MKLP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000137807.13
HGNCiHGNC:6392 KIF23
MIMi605064 gene
neXtProtiNX_Q02241

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9493
MalaCardsiKIF23
OpenTargetsiENSG00000137807
Orphaneti98870 Congenital dyserythropoietic anemia type III
PharmGKBiPA30181

Chemistry databases

ChEMBLiCHEMBL5899

Polymorphism and mutation databases

BioMutaiKIF23
DMDMi118572664

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001254341 – 960Kinesin-like protein KIF23Add BLAST960

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei155PhosphoserineCombined sources1
Modified residuei160PhosphoserineCombined sources1
Cross-linki571Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki586Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei605PhosphoserineCombined sources1
Cross-linki624Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki647Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki662Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki665Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei682PhosphoserineBy similarity1
Modified residuei684PhosphoserineBy similarity1
Modified residuei738PhosphothreonineCombined sources1
Cross-linki741Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei814PhosphoserineCombined sources1
Cross-linki823Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki854Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei867PhosphoserineCombined sources1
Cross-linki874Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki877Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki899Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei902PhosphoserineCombined sources1
Modified residuei911PhosphoserineCombined sources1
Modified residuei927PhosphothreonineCombined sources1
Cross-linki956Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Ubiquitinated. Deubiquitinated by USP8/UBPY.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ02241
MaxQBiQ02241
PaxDbiQ02241
PeptideAtlasiQ02241
PRIDEiQ02241

PTM databases

iPTMnetiQ02241
PhosphoSitePlusiQ02241

Expressioni

Gene expression databases

BgeeiENSG00000137807
CleanExiHS_KIF23
ExpressionAtlasiQ02241 baseline and differential
GenevisibleiQ02241 HS

Organism-specific databases

HPAiCAB010414
HPA045208
HPA068831

Interactioni

Subunit structurei

Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY. Interacts with ARF6, forming heterodimers and heterotetramers.8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • microtubule binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114873, 70 interactors
CORUMiQ02241
DIPiDIP-40359N
IntActiQ02241, 39 interactors
MINTiQ02241
STRINGi9606.ENSP00000260363

Chemistry databases

BindingDBiQ02241

Structurei

Secondary structure

1960
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi814 – 816Combined sources3
Beta strandi819 – 822Combined sources4
Beta strandi833 – 836Combined sources4
Beta strandi841 – 846Combined sources6
Helixi849 – 854Combined sources6
Beta strandi856 – 865Combined sources10
Beta strandi871 – 882Combined sources12
Beta strandi888 – 901Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VHXX-ray2.81B/D/F/H794-911[»]
ProteinModelPortaliQ02241
SMRiQ02241
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 436Kinesin motorPROSITE-ProRule annotationAdd BLAST412

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni794 – 911Interaction with ARF61 PublicationAdd BLAST118

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili535 – 620Sequence analysisAdd BLAST86

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 11Nuclear localization signalSequence analysis5

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0247 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00870000136438
HOGENOMiHOG000273893
HOVERGENiHBG052253
InParanoidiQ02241
KOiK17387
OrthoDBiEOG091G024C
PhylomeDBiQ02241
TreeFamiTF105232

Family and domain databases

Gene3Di3.40.50.12000, 1 hit
3.40.850.10, 2 hits
InterProiView protein in InterPro
IPR032924 KIF23
IPR032384 Kif23_Arf-bd
IPR038105 Kif23_Arf-bd_sf
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PTHR24115:SF467 PTHR24115:SF467, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PF16540 MKLP1_Arf_bdg, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q02241-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSARAKTPR KPTVKKGSQT NLKDPVGVYC RVRPLGFPDQ ECCIEVINNT
60 70 80 90 100
TVQLHTPEGY RLNRNGDYKE TQYSFKQVFG THTTQKELFD VVANPLVNDL
110 120 130 140 150
IHGKNGLLFT YGVTGSGKTH TMTGSPGEGG LLPRCLDMIF NSIGSFQAKR
160 170 180 190 200
YVFKSNDRNS MDIQCEVDAL LERQKREAMP NPKTSSSKRQ VDPEFADMIT
210 220 230 240 250
VQEFCKAEEV DEDSVYGVFV SYIEIYNNYI YDLLEEVPFD PIKPKPPQSK
260 270 280 290 300
LLREDKNHNM YVAGCTEVEV KSTEEAFEVF WRGQKKRRIA NTHLNRESSR
310 320 330 340 350
SHSVFNIKLV QAPLDADGDN VLQEKEQITI SQLSLVDLAG SERTNRTRAE
360 370 380 390 400
GNRLREAGNI NQSLMTLRTC MDVLRENQMY GTNKMVPYRD SKLTHLFKNY
410 420 430 440 450
FDGEGKVRMI VCVNPKAEDY EENLQVMRFA EVTQEVEVAR PVDKAICGLT
460 470 480 490 500
PGRRYRNQPR GPVGNEPLVT DVVLQSFPPL PSCEILDIND EQTLPRLIEA
510 520 530 540 550
LEKRHNLRQM MIDEFNKQSN AFKALLQEFD NAVLSKENHM QGKLNEKEKM
560 570 580 590 600
ISGQKLEIER LEKKNKTLEY KIEILEKTTT IYEEDKRNLQ QELETQNQKL
610 620 630 640 650
QRQFSDKRRL EARLQGMVTE TTMKWEKECE RRVAAKQLEM QNKLWVKDEK
660 670 680 690 700
LKQLKAIVTE PKTEKPERPS RERDREKVTQ RSVSPSPVPL SSNYIAQISN
710 720 730 740 750
GQQLMSQPQL HRRSNSCSSI SVASCISEWE QKIPTYNTPL KVTSIARRRQ
760 770 780 790 800
QEPGQSKTCI VSDRRRGMYW TEGREVVPTF RNEIEIEEDH CGRLLFQPDQ
810 820 830 840 850
NAPPIRLRHR RSRSAGDRWV DHKPASNMQT ETVMQPHVPH AITVSVANEK
860 870 880 890 900
ALAKCEKYML THQELASDGE IETKLIKGDI YKTRGGGQSV QFTDIETLKQ
910 920 930 940 950
ESPNGSRKRR SSTVAPAQPD GAESEWTDVE TRCSVAVEMR AGSQLGPGYQ
960
HHAQPKRKKP
Length:960
Mass (Da):110,059
Last modified:November 28, 2006 - v3
Checksum:iB2AA784D2D236A90
GO
Isoform 2 (identifier: Q02241-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     690-793: Missing.

Show »
Length:856
Mass (Da):98,105
Checksum:iDDE7CEB480ABE58A
GO
Isoform 3 (identifier: Q02241-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-197: Missing.
     245-245: K → KWNSCSTPMRNTDFV
     690-793: Missing.

Note: No experimental confirmation available.
Show »
Length:673
Mass (Da):77,699
Checksum:iF231A82604D60888
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049686515F → L. Corresponds to variant dbSNP:rs17310879Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0573481 – 197Missing in isoform 3. 1 PublicationAdd BLAST197
Alternative sequenceiVSP_057349245K → KWNSCSTPMRNTDFV in isoform 3. 1 Publication1
Alternative sequenceiVSP_021801690 – 793Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST104

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67155 mRNA Translation: CAA47628.2
AK303874 mRNA Translation: BAG64812.1
AC027237 Genomic DNA No translation available.
BC017705 mRNA Translation: AAH17705.2
BC051826 mRNA Translation: AAH51826.1
CCDSiCCDS32278.1 [Q02241-1]
CCDS32279.1 [Q02241-2]
PIRiS28262
RefSeqiNP_004847.2, NM_004856.6 [Q02241-2]
NP_612565.1, NM_138555.3 [Q02241-1]
UniGeneiHs.270845

Genome annotation databases

EnsembliENST00000260363; ENSP00000260363; ENSG00000137807 [Q02241-1]
ENST00000352331; ENSP00000304978; ENSG00000137807 [Q02241-2]
ENST00000558585; ENSP00000453102; ENSG00000137807 [Q02241-3]
ENST00000559279; ENSP00000453386; ENSG00000137807 [Q02241-2]
GeneIDi9493
KEGGihsa:9493
UCSCiuc002asb.5 human [Q02241-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKIF23_HUMAN
AccessioniPrimary (citable) accession number: Q02241
Secondary accession number(s): B4E1K0, Q8WVP0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 28, 2006
Last modified: May 23, 2018
This is version 185 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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