ID   GVPO_HALMT              Reviewed;         140 AA.
AC   Q02240; I3R589;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   27-MAR-2024, entry version 81.
DE   RecName: Full=Gas vesicle protein O;
DE            Short=GvpO {ECO:0000303|PubMed:1404376};
GN   Name=gvpO {ECO:0000303|PubMed:1404376}; OrderedLocusNames=HFX_1693;
OS   Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC
OS   14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=523841;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], GAS VESICLE GENE CLUSTER, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 /
RC   R-4;
RX   PubMed=1404376; DOI=10.1016/0022-2836(92)90914-6;
RA   Englert C., Krueger K., Offner S., Pfeifer F.;
RT   "Three different but related gene clusters encoding gas vesicles in
RT   halophilic archaea.";
RL   J. Mol. Biol. 227:586-592(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 /
RC   R-4;
RX   PubMed=22843593; DOI=10.1128/jb.00880-12;
RA   Han J., Zhang F., Hou J., Liu X., Li M., Liu H., Cai L., Zhang B., Chen Y.,
RA   Zhou J., Hu S., Xiang H.;
RT   "Complete genome sequence of the metabolically versatile halophilic
RT   archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-
RT   hydroxyvalerate) producer.";
RL   J. Bacteriol. 194:4463-4464(2012).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=1282192; DOI=10.1111/j.1365-2958.1992.tb01789.x;
RA   Englert C., Wanner G., Pfeifer F.;
RT   "Functional analysis of the gas vesicle gene cluster of the halophilic
RT   archaeon Haloferax mediterranei defines the vac-region boundary and
RT   suggests a regulatory role for the gvpD gene or its product.";
RL   Mol. Microbiol. 6:3543-3550(1992).
RN   [4]
RP   INDUCTION.
RX   PubMed=7683649; DOI=10.1016/s0021-9258(18)98354-7;
RA   Englert C., Pfeifer F.;
RT   "Analysis of gas vesicle gene expression in Haloferax mediterranei reveals
RT   that GvpA and GvpC are both gas vesicle structural proteins.";
RL   J. Biol. Chem. 268:9329-9336(1993).
RN   [5]
RP   INDUCTION BY SALT.
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 /
RC   R-4;
RX   PubMed=8757736; DOI=10.1099/13500872-142-7-1715;
RA   Roeder R., Pfeifer F.;
RT   "Influence of salt on the transcription of the gas-vesicle genes of
RT   Haloferax mediterranei and identification of the endogenous transcriptional
RT   activator gene.";
RL   Microbiology 142:1715-1723(1996).
CC   -!- FUNCTION: A minor component of the gas vesicle (GV), may play a role in
CC       transcription and/or RNA stability and/or in GV assembly. Gas vesicles
CC       are small, hollow, gas filled protein structures found in some
CC       microorganisms. They allow positioning of halobacteria at the optimal
CC       depth for growth in the poorly aerated shallow brine pools of their
CC       habitat. {ECO:0000250|UniProtKB:O51968}.
CC   -!- FUNCTION: Expression of a 9.5 kb mc-vac DNA fragment containing 2
CC       divergently transcribed regions (gvpD-gvpE-gvpF-gvpG-gvpH-gvpI-gvpJ-
CC       gvpK-gvpL-gvpM and gvpA-gvpC-gvpN-gvpO) allows H.volcanii to produce
CC       gas vesicles. {ECO:0000269|PubMed:1282192, ECO:0000269|PubMed:1404376,
CC       ECO:0000269|PubMed:8757736}.
CC   -!- SUBUNIT: Forms homodimers, forms a GvpN-GvpO heterodimer, interacts
CC       with GvpC, GvpF, GvpI and GvpL, might interact with GvpA.
CC       {ECO:0000250|UniProtKB:O51968}.
CC   -!- SUBCELLULAR LOCATION: Gas vesicle {ECO:0000250|UniProtKB:O51968}.
CC       Cytoplasm {ECO:0000250|UniProtKB:O51968}.
CC   -!- INDUCTION: Expressed from a single promoter upstream of gvpA; most
CC       transcripts stop at the gvpA terminator, with low read-through into
CC       downstream gvpC-gvpN-gvpO. Expression starts in early stationary phase
CC       and is maximal in stationary phase (PubMed:7683649, PubMed:8757736).
CC       Highly expressed in 25% salt, poorly expressed in 15% salt, no gas
CC       vesicles are formed at 15% salt (PubMed:8757736).
CC       {ECO:0000269|PubMed:7683649, ECO:0000269|PubMed:8757736}.
CC   -!- DISRUPTION PHENOTYPE: No longer makes gas vesicles in H.volcanii; cells
CC       translate GvpA but do not make gas vesicles.
CC       {ECO:0000269|PubMed:1282192, ECO:0000269|PubMed:1404376}.
CC   -!- MISCELLANEOUS: Encoded in a 14-gene locus called mc-vac.
CC       {ECO:0000269|PubMed:1282192, ECO:0000269|PubMed:1404376}.
CC   -!- SIMILARITY: Belongs to the gas vesicle GvpO family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X64701; CAA45942.1; -; Genomic_DNA.
DR   EMBL; CP001868; AFK19399.1; -; Genomic_DNA.
DR   PIR; S28113; S28113.
DR   RefSeq; WP_004056709.1; NZ_CP039139.1.
DR   AlphaFoldDB; Q02240; -.
DR   STRING; 523841.HFX_1693; -.
DR   PaxDb; 523841-HFX_1693; -.
DR   GeneID; 40157048; -.
DR   KEGG; hme:HFX_1693; -.
DR   eggNOG; arCOG04040; Archaea.
DR   HOGENOM; CLU_142302_0_0_2; -.
DR   OrthoDB; 205220at2157; -.
DR   Proteomes; UP000006469; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0031411; C:gas vesicle; IEA:UniProtKB-SubCell.
DR   GO; GO:0031412; P:gas vesicle organization; IEA:InterPro.
DR   InterPro; IPR008634; Gas-vesicle_GvpO.
DR   Pfam; PF05800; GvpO; 1.
DR   PIRSF; PIRSF028743; GvpO_protein; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Gas vesicle.
FT   CHAIN           1..140
FT                   /note="Gas vesicle protein O"
FT                   /id="PRO_0000182701"
FT   REGION          1..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        41..57
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   140 AA;  15307 MW;  B97A2A43E3B56BF4 CRC64;
     MSDQGNEHAN HDGIDNAGLS AEVGSGPEAK RTRDEPPEQT ASDEAVSNQS PDSTIGLSDA
     QVRAREAAKE LLEYEFEGII KIEAANGDGW RTVVELVERN AVPDTQDIIG RYEITLDATG
     SVTGYELLER YRRGDMKEEL
//