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Q02219

- ANIA_NEIGO

UniProt

Q02219 - ANIA_NEIGO

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Protein
Copper-containing nitrite reductase
Gene
aniA
Organism
Neisseria gonorrhoeae
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of nitrite to nitric oxide (NO), probably with azurin as electron donor. Essential for growth and survival in oxygen-depleted environments. Can also provide protection against killing by normal human sera.1 Publication

Catalytic activityi

Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.

Cofactori

Binds 1 Cu+ ion.
Binds 1 Cu2+ ion.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi134 – 1341Copper 1; type 1
Metal bindingi139 – 1391Copper 2; type 2
Binding sitei139 – 1391Substrate
Metal bindingi174 – 1741Copper 2; type 2
Metal bindingi175 – 1751Copper 1; type 1
Metal bindingi183 – 1831Copper 1; type 1
Metal bindingi188 – 1881Copper 1; type 1
Binding sitei280 – 2801Substrate
Metal bindingi329 – 3291Copper 2; type 2

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. nitrite reductase (NO-forming) activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Copper-containing nitrite reductase (EC:1.7.2.1)
Alternative name(s):
Major outer membrane protein Pan 1
Gene namesi
Name:aniA
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Subcellular locationi

GO - Cellular componenti

  1. cell outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Inferred
Add
BLAST
Chaini19 – 392374Copper-containing nitrite reductase
PRO_0000002997Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi19 – 191N-palmitoyl cysteine Inferred
Lipidationi19 – 191S-diacylglycerol cysteine Inferred

Post-translational modificationi

Palmitoylated.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate

Expressioni

Inductioni

By anaerobic and microaerophilic conditions in the presence of nitrite. Regulated by the gonococcal fnr and NarP homologs.3 Publications

Interactioni

Subunit structurei

Homotrimer.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 583
Beta strandi78 – 9316
Beta strandi96 – 1038
Beta strandi106 – 1083
Beta strandi111 – 1155
Beta strandi119 – 1268
Helixi144 – 1474
Turni148 – 1514
Beta strandi157 – 1648
Beta strandi169 – 1746
Helixi180 – 1856
Beta strandi189 – 1957
Beta strandi204 – 21411
Beta strandi216 – 2183
Beta strandi224 – 2263
Helixi230 – 2356
Beta strandi239 – 2435
Turni247 – 2504
Helixi252 – 2543
Beta strandi256 – 2594
Beta strandi262 – 27413
Beta strandi277 – 2826
Beta strandi286 – 2905
Helixi291 – 2933
Beta strandi300 – 3067
Beta strandi310 – 3189
Beta strandi322 – 3309
Helixi332 – 3365
Beta strandi340 – 3478
Turni351 – 3533

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KBVX-ray1.95A/B/C/D/E/F42-364[»]
1KBWX-ray2.40A/B/C/D/E/F42-364[»]
ProteinModelPortaliQ02219.
SMRiQ02219. Positions 53-354.

Miscellaneous databases

EvolutionaryTraceiQ02219.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 19595Plastocyanin-like 1
Add
BLAST
Domaini245 – 346102Plastocyanin-like 2
Add
BLAST
Repeati368 – 37251
Repeati373 – 37752
Repeati378 – 38253
Repeati383 – 38754

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni368 – 387204 X 5 AA tandem repeats of A-A-S-A-P
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.40.420. 2 hits.
InterProiIPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR001287. NO2-reductase_Cu.
[Graphical view]
PfamiPF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
PRINTSiPR00695. CUNO2RDTASE.
SUPFAMiSSF49503. SSF49503. 2 hits.
TIGRFAMsiTIGR02376. Cu_nitrite_red. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02219-1 [UniParc]FASTAAdd to Basket

« Hide

MKRQALAAMI ASLFALAACG GEQAAQAPAE TPAASAEAAS SAAQATAETP    50
AGELPVIDAV TTHAPEVPPA IDRDYPAKVR VKMETVEKTM KMDDGVEYRY 100
WTFDGDVPGR MIRVREGDTV EVEFSNNPSS TVPHNVDFHA ATGQGGGAAA 150
TFTAPGRTST FSFKALQPGL YIYHCAVAPV GMHIANGMYG LILVEPKEGL 200
PKVDKEFYIV QGDFYTKGKK GAQGLQPFDM DKAVAEQPEY VVFNGHVGSI 250
AGDNALKAKA GETVRMYVGN GGPNLVSSFH VIGEIFDKVY VEGGKLINEN 300
VQSTIVPAGG SAIVEFKVDI PGSYTLVDHS IFRAFNKGAL GQLKVEGAEN 350
PEIMTQKLSD TAYAGSGAAS APAASAPAAS APAASASEKS VY 392
Length:392
Mass (Da):40,954
Last modified:June 1, 1994 - v1
Checksum:iA4707CC87B923C97
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97926 Genomic DNA. Translation: AAA25462.1.
PIRiA49208.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97926 Genomic DNA. Translation: AAA25462.1 .
PIRi A49208.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KBV X-ray 1.95 A/B/C/D/E/F 42-364 [» ]
1KBW X-ray 2.40 A/B/C/D/E/F 42-364 [» ]
ProteinModelPortali Q02219.
SMRi Q02219. Positions 53-354.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q02219.

Family and domain databases

Gene3Di 2.60.40.420. 2 hits.
InterProi IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR001287. NO2-reductase_Cu.
[Graphical view ]
Pfami PF07732. Cu-oxidase_3. 1 hit.
[Graphical view ]
PRINTSi PR00695. CUNO2RDTASE.
SUPFAMi SSF49503. SSF49503. 2 hits.
TIGRFAMsi TIGR02376. Cu_nitrite_red. 1 hit.
PROSITEi PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and nucleotide sequence of the gene (aniA) encoding the major anaerobically induced outer membrane protein of Neisseria gonorrhoeae."
    Hoehn G.T., Clark V.L.
    Infect. Immun. 60:4695-4703(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: R10.
  2. "The major anaerobically induced outer membrane protein of Neisseria gonorrhoeae, Pan 1, is a lipoprotein."
    Hoehn G.T., Clark V.L.
    Infect. Immun. 60:4704-4708(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-19, DIACYLGLYCEROL AT CYS-19.
    Strain: ATCC 33084 / F62 / M-1914.
  3. "The Neisseria gonorrhoeae gene aniA encodes an inducible nitrite reductase."
    Mellies J., Jose J., Meyer T.F.
    Mol. Gen. Genet. 256:525-532(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
    Strain: MS11.
  4. "cis- and trans-acting elements involved in regulation of aniA, the gene encoding the major anaerobically induced outer membrane protein in Neisseria gonorrhoeae."
    Householder T.C., Belli W.A., Lissenden S., Cole J.A., Clark V.L.
    J. Bacteriol. 181:541-551(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSCRIPTIONAL REGULATION BY FNR AND NARP.
    Strain: ATCC 33084 / F62 / M-1914.
  5. "Expression of AniA, the major anaerobically induced outer membrane protein of Neisseria gonorrhoeae, provides protection against killing by normal human sera."
    Cardinale J.A., Clark V.L.
    Infect. Immun. 68:4368-4369(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTECTION AGAINST KILLING BY HUMAN SERA.
    Strain: ATCC 33084 / F62 / M-1914.
  6. "Crystal structure of the soluble domain of the major anaerobically induced outer membrane protein (AniA) from pathogenic Neisseria: a new class of copper-containing nitrite reductases."
    Boulanger M.J., Murphy M.E.P.
    J. Mol. Biol. 315:1111-1127(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 42-364 IN COMPLEX WITH SUBSTRATE AND COPPER IONS, SUBUNIT.

Entry informationi

Entry nameiANIA_NEIGO
AccessioniPrimary (citable) accession number: Q02219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 13, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Undetected during aerobic growth.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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