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Q02219

- ANIA_NEIGO

UniProt

Q02219 - ANIA_NEIGO

Protein

Copper-containing nitrite reductase

Gene

aniA

Organism
Neisseria gonorrhoeae
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of nitrite to nitric oxide (NO), probably with azurin as electron donor. Essential for growth and survival in oxygen-depleted environments. Can also provide protection against killing by normal human sera.1 Publication

    Catalytic activityi

    Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.

    Cofactori

    Binds 1 Cu+ ion.
    Binds 1 Cu2+ ion.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi134 – 1341Copper 1; type 1
    Metal bindingi139 – 1391Copper 2; type 2
    Binding sitei139 – 1391Substrate1 Publication
    Metal bindingi174 – 1741Copper 2; type 2
    Metal bindingi175 – 1751Copper 1; type 1
    Metal bindingi183 – 1831Copper 1; type 1
    Metal bindingi188 – 1881Copper 1; type 1
    Binding sitei280 – 2801Substrate1 Publication
    Metal bindingi329 – 3291Copper 2; type 2

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. nitrite reductase (NO-forming) activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Copper-containing nitrite reductase (EC:1.7.2.1)
    Alternative name(s):
    Major outer membrane protein Pan 1
    Gene namesi
    Name:aniA
    OrganismiNeisseria gonorrhoeae
    Taxonomic identifieri485 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

    Subcellular locationi

    GO - Cellular componenti

    1. cell outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818CuratedAdd
    BLAST
    Chaini19 – 392374Copper-containing nitrite reductasePRO_0000002997Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi19 – 191N-palmitoyl cysteine1 Publication
    Lipidationi19 – 191S-diacylglycerol cysteine1 Publication

    Post-translational modificationi

    Palmitoylated.1 Publication

    Keywords - PTMi

    Lipoprotein, Palmitate

    Expressioni

    Inductioni

    By anaerobic and microaerophilic conditions in the presence of nitrite. Regulated by the gonococcal fnr and NarP homologs.3 Publications

    Interactioni

    Subunit structurei

    Homotrimer.1 Publication

    Structurei

    Secondary structure

    1
    392
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi56 – 583
    Beta strandi78 – 9316
    Beta strandi96 – 1038
    Beta strandi106 – 1083
    Beta strandi111 – 1155
    Beta strandi119 – 1268
    Helixi144 – 1474
    Turni148 – 1514
    Beta strandi157 – 1648
    Beta strandi169 – 1746
    Helixi180 – 1856
    Beta strandi189 – 1957
    Beta strandi204 – 21411
    Beta strandi216 – 2183
    Beta strandi224 – 2263
    Helixi230 – 2356
    Beta strandi239 – 2435
    Turni247 – 2504
    Helixi252 – 2543
    Beta strandi256 – 2594
    Beta strandi262 – 27413
    Beta strandi277 – 2826
    Beta strandi286 – 2905
    Helixi291 – 2933
    Beta strandi300 – 3067
    Beta strandi310 – 3189
    Beta strandi322 – 3309
    Helixi332 – 3365
    Beta strandi340 – 3478
    Turni351 – 3533

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KBVX-ray1.95A/B/C/D/E/F42-364[»]
    1KBWX-ray2.40A/B/C/D/E/F42-364[»]
    ProteinModelPortaliQ02219.
    SMRiQ02219. Positions 53-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ02219.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini101 – 19595Plastocyanin-like 1Add
    BLAST
    Domaini245 – 346102Plastocyanin-like 2Add
    BLAST
    Repeati368 – 37251
    Repeati373 – 37752
    Repeati378 – 38253
    Repeati383 – 38754

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni368 – 387204 X 5 AA tandem repeats of A-A-S-A-PAdd
    BLAST

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Curated
    Contains 2 plastocyanin-like domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.60.40.420. 2 hits.
    InterProiIPR011707. Cu-oxidase_3.
    IPR008972. Cupredoxin.
    IPR001287. NO2-reductase_Cu.
    [Graphical view]
    PfamiPF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    PRINTSiPR00695. CUNO2RDTASE.
    SUPFAMiSSF49503. SSF49503. 2 hits.
    TIGRFAMsiTIGR02376. Cu_nitrite_red. 1 hit.
    PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q02219-1 [UniParc]FASTAAdd to Basket

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    MKRQALAAMI ASLFALAACG GEQAAQAPAE TPAASAEAAS SAAQATAETP    50
    AGELPVIDAV TTHAPEVPPA IDRDYPAKVR VKMETVEKTM KMDDGVEYRY 100
    WTFDGDVPGR MIRVREGDTV EVEFSNNPSS TVPHNVDFHA ATGQGGGAAA 150
    TFTAPGRTST FSFKALQPGL YIYHCAVAPV GMHIANGMYG LILVEPKEGL 200
    PKVDKEFYIV QGDFYTKGKK GAQGLQPFDM DKAVAEQPEY VVFNGHVGSI 250
    AGDNALKAKA GETVRMYVGN GGPNLVSSFH VIGEIFDKVY VEGGKLINEN 300
    VQSTIVPAGG SAIVEFKVDI PGSYTLVDHS IFRAFNKGAL GQLKVEGAEN 350
    PEIMTQKLSD TAYAGSGAAS APAASAPAAS APAASASEKS VY 392
    Length:392
    Mass (Da):40,954
    Last modified:June 1, 1994 - v1
    Checksum:iA4707CC87B923C97
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97926 Genomic DNA. Translation: AAA25462.1.
    PIRiA49208.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97926 Genomic DNA. Translation: AAA25462.1 .
    PIRi A49208.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KBV X-ray 1.95 A/B/C/D/E/F 42-364 [» ]
    1KBW X-ray 2.40 A/B/C/D/E/F 42-364 [» ]
    ProteinModelPortali Q02219.
    SMRi Q02219. Positions 53-354.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q02219.

    Family and domain databases

    Gene3Di 2.60.40.420. 2 hits.
    InterProi IPR011707. Cu-oxidase_3.
    IPR008972. Cupredoxin.
    IPR001287. NO2-reductase_Cu.
    [Graphical view ]
    Pfami PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00695. CUNO2RDTASE.
    SUPFAMi SSF49503. SSF49503. 2 hits.
    TIGRFAMsi TIGR02376. Cu_nitrite_red. 1 hit.
    PROSITEi PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and nucleotide sequence of the gene (aniA) encoding the major anaerobically induced outer membrane protein of Neisseria gonorrhoeae."
      Hoehn G.T., Clark V.L.
      Infect. Immun. 60:4695-4703(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
      Strain: R10.
    2. "The major anaerobically induced outer membrane protein of Neisseria gonorrhoeae, Pan 1, is a lipoprotein."
      Hoehn G.T., Clark V.L.
      Infect. Immun. 60:4704-4708(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-19, DIACYLGLYCEROL AT CYS-19.
      Strain: ATCC 33084 / F62 / M-1914.
    3. "The Neisseria gonorrhoeae gene aniA encodes an inducible nitrite reductase."
      Mellies J., Jose J., Meyer T.F.
      Mol. Gen. Genet. 256:525-532(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
      Strain: MS11.
    4. "cis- and trans-acting elements involved in regulation of aniA, the gene encoding the major anaerobically induced outer membrane protein in Neisseria gonorrhoeae."
      Householder T.C., Belli W.A., Lissenden S., Cole J.A., Clark V.L.
      J. Bacteriol. 181:541-551(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: TRANSCRIPTIONAL REGULATION BY FNR AND NARP.
      Strain: ATCC 33084 / F62 / M-1914.
    5. "Expression of AniA, the major anaerobically induced outer membrane protein of Neisseria gonorrhoeae, provides protection against killing by normal human sera."
      Cardinale J.A., Clark V.L.
      Infect. Immun. 68:4368-4369(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTECTION AGAINST KILLING BY HUMAN SERA.
      Strain: ATCC 33084 / F62 / M-1914.
    6. "Crystal structure of the soluble domain of the major anaerobically induced outer membrane protein (AniA) from pathogenic Neisseria: a new class of copper-containing nitrite reductases."
      Boulanger M.J., Murphy M.E.P.
      J. Mol. Biol. 315:1111-1127(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 42-364 IN COMPLEX WITH SUBSTRATE AND COPPER IONS, SUBUNIT.

    Entry informationi

    Entry nameiANIA_NEIGO
    AccessioniPrimary (citable) accession number: Q02219
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Undetected during aerobic growth.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3