Reviewed, UniProtKB/Swiss-Prot Q02216 (MASY_CANTR)

Names and origin

Protein names

Recommended name:
    Malate synthase, glyoxysomal
    EC=2.3.3.9

Gene names

Name:	PMS1
AND
Name:	PMS2

Organism

Candida tropicalis (Yeast)

Taxonomic identifier

5482 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subcellular location	Glyoxysome.
Miscellaneous	There are two genes for malate synthase in C.tropicalis. The sequence shown is that of PMS1, PMS2 differs at a single position.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Transferase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	Malate synthase, glyoxysomal		PRO_0000166860
Sites
	Active site	174	1	Proton acceptor By similarity
	Active site	458	1	Proton donor By similarity
Natural variations
	Natural variant	159	1	N → S in PMS2.

Sequences

	Sequence		Length	Mass (Da)
	Q02216-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: A74A587A9F22C8EC	FASTA	551	62,449
	10 20 30 40 50 60 MSTPFPKTAD KVKGVQILGP IPDEAKHIFN QETLAFVATL HRGFEARRQE LLNNRKEQQK 70 80 90 100 110 120 LRDQGFLPDF LPETEYIRND STWTGPALAP GLIDRRCEIT GPTDRKMVIN ALNSNVATYM 130 140 150 160 170 180 ADFEDSLTPA WKNLVEGQVN LYDAVRRNLS ATINGKQYNL NLEKGRHIPT LIVRPRGWHL 190 200 210 220 230 240 TEKHVLVDGT PVSGGIFDFA VYFYNSAKEA IAQGFGPYFY LPKMEHHLEA KLWNDIFNYS 250 260 270 280 290 300 QDYIGLKRGT IRASVLIETI PAVFQMDEII YQLREHSAGL NCGRWDYIFS YIKCLRNHPD 310 320 330 340 350 360 FILPDRSQVT MAAPFMSSYV KLLVHTTHKR KVHALGGMAA QIPIKDDEAR NRAALENVTK 370 380 390 400 410 420 DKLREVTLGC DSCWVAHPAL VPVVLKVFNE HMKGPNQISL PPKEPFKPIT QRDLLSPFVP 430 440 450 460 470 480 GAKITEQGIR ANIVIGISYI EAWLRNVGCV PINYLMEDAA TAEVSRTQIW QWVTHGAKTD 490 500 510 520 530 540 TGKVITKEYV KQLLDEEYAK LTKNAKPGNK FKRAFEYFAP EALGEKYSDF VTTLIYDDVT 550 TIGRALPGER L

References

[1]	"Presence of two transcribed malate synthase genes in an n-alkane-utilizing yeast, Candida tropicalis." Hikada M., Atomi H., Fukuda Y., Aoki A., Hishida T., Teranishi Y., Ueda M., Tanaka A. J. Biochem. 110:909-914(1991) [PubMed: 1794980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13415 Genomic DNA. Translation: BAA02680.1. D13416 Genomic DNA. Translation: BAA02681.1.
PIR	JX0195. JX0196.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.3.9. 1242.
Family and domain databases
InterPro	IPR011076. Malate_synth-like_core. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_CANTR

Accession

Primary (citable) accession number: Q02216

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	October 13, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)