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Q02207

- FOX2_YEAST

UniProt

Q02207 - FOX2_YEAST

Protein

Peroxisomal hydratase-dehydrogenase-epimerase

Gene

FOX2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Second trifunctional enzyme acting on the beta-oxidation pathway for fatty acids, possessing hydratase-dehydrogenase-epimerase activities. Converts trans-2-enoyl-CoA via D-3-hydroxyacyl-CoA to 3-ketoacyl-CoA.

    Catalytic activityi

    (3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H2O.
    (R)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei21 – 211NAD; via amide nitrogenBy similarity
    Binding sitei40 – 401NADBy similarity
    Binding sitei100 – 1001NAD; via carbonyl oxygenBy similarity
    Binding sitei152 – 1521SubstrateBy similarity
    Active sitei165 – 1651Proton acceptorPROSITE-ProRule annotation
    Binding sitei456 – 4561SubstrateBy similarity
    Active sitei469 – 4691Proton acceptorPROSITE-ProRule annotation
    Binding sitei719 – 7191(3R)-3-hydroxydecanoyl-CoABy similarity
    Binding sitei826 – 8261(3R)-3-hydroxydecanoyl-CoA; via amide nitrogenBy similarity
    Binding sitei851 – 8511(3R)-3-hydroxydecanoyl-CoA; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 3725NADBy similarityAdd
    BLAST
    Nucleotide bindingi75 – 762NADBy similarity
    Nucleotide bindingi165 – 1695NADBy similarity
    Nucleotide bindingi197 – 2004NADBy similarity
    Nucleotide bindingi326 – 35025NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: SGD
    2. enoyl-CoA hydratase activity Source: SGD
    3. isomerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. fatty acid beta-oxidation Source: SGD

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:YKR009C-MONOMER.
    YEAST:YKR009C-MONOMER.
    ReactomeiREACT_188947. alpha-linolenic acid (ALA) metabolism.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal hydratase-dehydrogenase-epimerase
    Short name:
    HDE
    Alternative name(s):
    Multifunctional beta-oxidation protein
    Short name:
    MFP
    Including the following 2 domains:
    2-enoyl-CoA hydratase (EC:4.2.1.119)
    (3R)-3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.n12)
    Gene namesi
    Name:FOX2
    Ordered Locus Names:YKR009C
    ORF Names:YK108
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR009c.
    SGDiS000001717. FOX2.

    Subcellular locationi

    GO - Cellular componenti

    1. peroxisome Source: SGD

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 900900Peroxisomal hydratase-dehydrogenase-epimerasePRO_0000054700Add
    BLAST

    Proteomic databases

    MaxQBiQ02207.
    PaxDbiQ02207.
    PeptideAtlasiQ02207.

    Expressioni

    Gene expression databases

    GenevestigatoriQ02207.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi34141. 34 interactions.
    IntActiQ02207. 1 interaction.
    MINTiMINT-2787193.
    STRINGi4932.YKR009C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02207.
    SMRiQ02207. Positions 5-893.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini775 – 887113MaoC-likeAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni6 – 230225Short-chain dehydrogenase like 1Add
    BLAST
    Regioni319 – 535217Short-chain dehydrogenase like 2Add
    BLAST
    Regioni689 – 6902(3R)-3-hydroxydecanoyl-CoA bindingBy similarity
    Regioni803 – 8086(3R)-3-hydroxydecanoyl-CoA bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi898 – 9003Microbody targeting signalSequence Analysis

    Domaini

    Contains two SDR domains.

    Sequence similaritiesi

    Contains 1 MaoC-like domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1028.
    GeneTreeiENSGT00530000062928.
    HOGENOMiHOG000170895.
    KOiK14729.
    OMAiTETIMPP.
    OrthoDBiEOG7HQNM0.

    Family and domain databases

    Gene3Di3.10.129.10. 2 hits.
    3.40.50.720. 2 hits.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR029069. HotDog_dom.
    IPR002539. MaoC_dom.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 2 hits.
    PF01575. MaoC_dehydratas. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMiSSF54637. SSF54637. 2 hits.
    PROSITEiPS00061. ADH_SHORT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02207-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGNLSFKDR VVVITGAGGG LGKVYALAYA SRGAKVVVND LGGTLGGSGH    50
    NSKAADLVVD EIKKAGGIAV ANYDSVNENG EKIIETAIKE FGRVDVLINN 100
    AGILRDVSFA KMTEREFASV VDVHLTGGYK LSRAAWPYMR SQKFGRIINT 150
    ASPAGLFGNF GQANYSAAKM GLVGLAETLA KEGAKYNINV NSIAPLARSR 200
    MTENVLPPHI LKQLGPEKIV PLVLYLTHES TKVSNSIFEL AAGFFGQLRW 250
    ERSSGQIFNP DPKTYTPEAI LNKWKEITDY RDKPFNKTQH PYQLSDYNDL 300
    ITKAKKLPPN EQGSVKIKSL CNKVVVVTGA GGGLGKSHAI WFARYGAKVV 350
    VNDIKDPFSV VEEINKLYGE GTAIPDSHDV VTEAPLIIQT AISKFQRVDI 400
    LVNNAGILRD KSFLKMKDEE WFAVLKVHLF STFSLSKAVW PIFTKQKSGF 450
    IINTTSTSGI YGNFGQANYA AAKAAILGFS KTIALEGAKR GIIVNVIAPH 500
    AETAMTKTIF SEKELSNHFD ASQVSPLVVL LASEELQKYS GRRVIGQLFE 550
    VGGGWCGQTR WQRSSGYVSI KETIEPEEIK ENWNHITDFS RNTINPSSTE 600
    ESSMATLQAV QKAHSSKELD DGLFKYTTKD CILYNLGLGC TSKELKYTYE 650
    NDPDFQVLPT FAVIPFMQAT ATLAMDNLVD NFNYAMLLHG EQYFKLCTPT 700
    MPSNGTLKTL AKPLQVLDKN GKAALVVGGF ETYDIKTKKL IAYNEGSFFI 750
    RGAHVPPEKE VRDGKRAKFA VQNFEVPHGK VPDFEAEIST NKDQAALYRL 800
    SGDFNPLHID PTLAKAVKFP TPILHGLCTL GISAKALFEH YGPYEELKVR 850
    FTNVVFPGDT LKVKAWKQGS VVVFQTIDTT RNVIVLDNAA VKLSQAKSKL 900
    Length:900
    Mass (Da):98,703
    Last modified:July 1, 1993 - v1
    Checksum:i66FFD0D49C673788
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86456 mRNA. Translation: AAA34779.1.
    X65124 Genomic DNA. Translation: CAA46243.1.
    Z28234 Genomic DNA. Translation: CAA82079.1.
    BK006944 Genomic DNA. Translation: DAA09165.1.
    PIRiS25322.
    RefSeqiNP_012934.1. NM_001179799.1.

    Genome annotation databases

    EnsemblFungiiYKR009C; YKR009C; YKR009C.
    GeneIDi853878.
    KEGGisce:YKR009C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86456 mRNA. Translation: AAA34779.1 .
    X65124 Genomic DNA. Translation: CAA46243.1 .
    Z28234 Genomic DNA. Translation: CAA82079.1 .
    BK006944 Genomic DNA. Translation: DAA09165.1 .
    PIRi S25322.
    RefSeqi NP_012934.1. NM_001179799.1.

    3D structure databases

    ProteinModelPortali Q02207.
    SMRi Q02207. Positions 5-893.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34141. 34 interactions.
    IntActi Q02207. 1 interaction.
    MINTi MINT-2787193.
    STRINGi 4932.YKR009C.

    Proteomic databases

    MaxQBi Q02207.
    PaxDbi Q02207.
    PeptideAtlasi Q02207.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR009C ; YKR009C ; YKR009C .
    GeneIDi 853878.
    KEGGi sce:YKR009C.

    Organism-specific databases

    CYGDi YKR009c.
    SGDi S000001717. FOX2.

    Phylogenomic databases

    eggNOGi COG1028.
    GeneTreei ENSGT00530000062928.
    HOGENOMi HOG000170895.
    KOi K14729.
    OMAi TETIMPP.
    OrthoDBi EOG7HQNM0.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci MetaCyc:YKR009C-MONOMER.
    YEAST:YKR009C-MONOMER.
    Reactomei REACT_188947. alpha-linolenic acid (ALA) metabolism.

    Miscellaneous databases

    NextBioi 975163.
    PROi Q02207.

    Gene expression databases

    Genevestigatori Q02207.

    Family and domain databases

    Gene3Di 3.10.129.10. 2 hits.
    3.40.50.720. 2 hits.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR029069. HotDog_dom.
    IPR002539. MaoC_dom.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 2 hits.
    PF01575. MaoC_dehydratas. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMi SSF54637. SSF54637. 2 hits.
    PROSITEi PS00061. ADH_SHORT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Peroxisomal multifunctional beta-oxidation protein of Saccharomyces cerevisiae. Molecular analysis of the fox2 gene and gene product."
      Hiltunen J.K., Wenzel B., Beyer A., Erdmann R., Fossa A., Kunau W.H.
      J. Biol. Chem. 267:6646-6653(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "DNA sequencing and analysis of a 24.7 kb segment encompassing centromere CEN11 of Saccharomyces cerevisiae reveals nine previously unknown open reading frames."
      Duesterhoeft A., Philippsen P.
      Yeast 8:749-759(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiFOX2_YEAST
    AccessioniPrimary (citable) accession number: Q02207
    Secondary accession number(s): D6VX75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3