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Protein

Peroxisomal hydratase-dehydrogenase-epimerase

Gene

FOX2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Second trifunctional enzyme acting on the beta-oxidation pathway for fatty acids, possessing hydratase-dehydrogenase-epimerase activities. Converts trans-2-enoyl-CoA via D-3-hydroxyacyl-CoA to 3-ketoacyl-CoA.

Catalytic activityi

(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H2O.
(R)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21NAD; via amide nitrogenBy similarity1
Binding sitei40NADBy similarity1
Binding sitei100NAD; via carbonyl oxygenBy similarity1
Binding sitei152SubstrateBy similarity1
Active sitei165Proton acceptorPROSITE-ProRule annotation1
Binding sitei456SubstrateBy similarity1
Active sitei469Proton acceptorPROSITE-ProRule annotation1
Binding sitei719(3R)-3-hydroxydecanoyl-CoABy similarity1
Binding sitei826(3R)-3-hydroxydecanoyl-CoA; via amide nitrogenBy similarity1
Binding sitei851(3R)-3-hydroxydecanoyl-CoA; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 37NADBy similarityAdd BLAST25
Nucleotide bindingi75 – 76NADBy similarity2
Nucleotide bindingi165 – 169NADBy similarity5
Nucleotide bindingi197 – 200NADBy similarity4
Nucleotide bindingi326 – 350NADBy similarityAdd BLAST25

GO - Molecular functioni

  • 3-hydroxyacyl-CoA dehydrogenase activity Source: SGD
  • enoyl-CoA hydratase activity Source: SGD
  • isomerase activity Source: UniProtKB-KW

GO - Biological processi

  • fatty acid beta-oxidation Source: SGD

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandNAD

Enzyme and pathway databases

BioCyciMetaCyc:YKR009C-MONOMER
YEAST:YKR009C-MONOMER
BRENDAi4.2.1.119 984
ReactomeiR-SCE-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-SCE-2046106 alpha-linolenic acid (ALA) metabolism
R-SCE-389887 Beta-oxidation of pristanoyl-CoA
R-SCE-390247 Beta-oxidation of very long chain fatty acids
R-SCE-9033241 Peroxisomal protein import
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal hydratase-dehydrogenase-epimerase
Short name:
HDE
Alternative name(s):
Multifunctional beta-oxidation protein
Short name:
MFP
Including the following 2 domains:
2-enoyl-CoA hydratase (EC:4.2.1.119)
(3R)-3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.n12)
Gene namesi
Name:FOX2
Ordered Locus Names:YKR009C
ORF Names:YK108
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR009C
SGDiS000001717 FOX2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000547001 – 900Peroxisomal hydratase-dehydrogenase-epimeraseAdd BLAST900

Proteomic databases

MaxQBiQ02207
PaxDbiQ02207
PRIDEiQ02207

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi34141, 61 interactors
IntActiQ02207, 1 interactor
MINTiQ02207
STRINGi4932.YKR009C

Structurei

3D structure databases

ProteinModelPortaliQ02207
SMRiQ02207
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini775 – 887MaoC-likeAdd BLAST113

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 230Short-chain dehydrogenase like 1Add BLAST225
Regioni319 – 535Short-chain dehydrogenase like 2Add BLAST217
Regioni689 – 690(3R)-3-hydroxydecanoyl-CoA bindingBy similarity2
Regioni803 – 808(3R)-3-hydroxydecanoyl-CoA bindingBy similarity6

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi898 – 900Microbody targeting signalSequence analysis3

Domaini

Contains two SDR domains.

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00530000062928
HOGENOMiHOG000170895
InParanoidiQ02207
KOiK14729
OMAiSRMTQTV
OrthoDBiEOG092C3TSH

Family and domain databases

InterProiView protein in InterPro
IPR029069 HotDog_dom_sf
IPR002539 MaoC_dom
IPR036291 NAD(P)-bd_dom_sf
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 2 hits
PF01575 MaoC_dehydratas, 1 hit
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 2 hits
SSF54637 SSF54637, 2 hits
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 2 hits

Sequencei

Sequence statusi: Complete.

Q02207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGNLSFKDR VVVITGAGGG LGKVYALAYA SRGAKVVVND LGGTLGGSGH
60 70 80 90 100
NSKAADLVVD EIKKAGGIAV ANYDSVNENG EKIIETAIKE FGRVDVLINN
110 120 130 140 150
AGILRDVSFA KMTEREFASV VDVHLTGGYK LSRAAWPYMR SQKFGRIINT
160 170 180 190 200
ASPAGLFGNF GQANYSAAKM GLVGLAETLA KEGAKYNINV NSIAPLARSR
210 220 230 240 250
MTENVLPPHI LKQLGPEKIV PLVLYLTHES TKVSNSIFEL AAGFFGQLRW
260 270 280 290 300
ERSSGQIFNP DPKTYTPEAI LNKWKEITDY RDKPFNKTQH PYQLSDYNDL
310 320 330 340 350
ITKAKKLPPN EQGSVKIKSL CNKVVVVTGA GGGLGKSHAI WFARYGAKVV
360 370 380 390 400
VNDIKDPFSV VEEINKLYGE GTAIPDSHDV VTEAPLIIQT AISKFQRVDI
410 420 430 440 450
LVNNAGILRD KSFLKMKDEE WFAVLKVHLF STFSLSKAVW PIFTKQKSGF
460 470 480 490 500
IINTTSTSGI YGNFGQANYA AAKAAILGFS KTIALEGAKR GIIVNVIAPH
510 520 530 540 550
AETAMTKTIF SEKELSNHFD ASQVSPLVVL LASEELQKYS GRRVIGQLFE
560 570 580 590 600
VGGGWCGQTR WQRSSGYVSI KETIEPEEIK ENWNHITDFS RNTINPSSTE
610 620 630 640 650
ESSMATLQAV QKAHSSKELD DGLFKYTTKD CILYNLGLGC TSKELKYTYE
660 670 680 690 700
NDPDFQVLPT FAVIPFMQAT ATLAMDNLVD NFNYAMLLHG EQYFKLCTPT
710 720 730 740 750
MPSNGTLKTL AKPLQVLDKN GKAALVVGGF ETYDIKTKKL IAYNEGSFFI
760 770 780 790 800
RGAHVPPEKE VRDGKRAKFA VQNFEVPHGK VPDFEAEIST NKDQAALYRL
810 820 830 840 850
SGDFNPLHID PTLAKAVKFP TPILHGLCTL GISAKALFEH YGPYEELKVR
860 870 880 890 900
FTNVVFPGDT LKVKAWKQGS VVVFQTIDTT RNVIVLDNAA VKLSQAKSKL
Length:900
Mass (Da):98,703
Last modified:July 1, 1993 - v1
Checksum:i66FFD0D49C673788
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86456 mRNA Translation: AAA34779.1
X65124 Genomic DNA Translation: CAA46243.1
Z28234 Genomic DNA Translation: CAA82079.1
BK006944 Genomic DNA Translation: DAA09165.1
PIRiS25322
RefSeqiNP_012934.1, NM_001179799.1

Genome annotation databases

EnsemblFungiiYKR009C; YKR009C; YKR009C
GeneIDi853878
KEGGisce:YKR009C

Similar proteinsi

Entry informationi

Entry nameiFOX2_YEAST
AccessioniPrimary (citable) accession number: Q02207
Secondary accession number(s): D6VX75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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