Q02205 (MEH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein MEH1

Alternative name(s):
EGO complex subunit 1
GSE complex subunit 2

Gene names

Name:	MEH1
Synonyms:	EGO1, GSE2
Ordered Locus Names:	YKR007W
ORF Names:	YK106

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Component of the EGO complex, a complex involved in the regulation of microautophagy. Ref.5 Ref.8
Subunit structure	Component of the GSE complex composed of GTR1, GTR2, SLM4, MEH1 and LTV1. Component of the EGO complex, at least composed of GTR2, SLM4 and MEH1.
Subcellular location	Vacuole membrane Ref.5.
Miscellaneous	Present with 2740 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Membrane Vacuole
Domain	Coiled coil
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	microautophagy Inferred from mutant phenotype Ref.5. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW vacuolar acidification Inferred from mutant phenotype. Source: SGD
Cellular component	GSE complex Inferred from direct assay Ref.8. Source: SGD fungal-type vacuole membrane Inferred from direct assay Ref.5. Source: SGD late endosome membrane Inferred from direct assay Ref.8. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GTR2	P53290	3	EBI-27062,EBI-7962
SLM4	P38247	4	EBI-27062,EBI-21507

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Probable
	Chain	2 – 184	183	Protein MEH1		PRO_0000203193
Regions
	Coiled coil	30 – 71	42	Potential
Amino acid modifications
	Modified residue	117	1	Phosphoserine Ref.10
	Modified residue	146	1	Phosphoserine Ref.6 Ref.9 Ref.11
	Modified residue	149	1	Phosphoserine Ref.9
	Lipidation	2	1	N-myristoyl glycine Probable
	Lipidation	7	1	S-palmitoyl cysteine Probable
	Lipidation	8	1	S-palmitoyl cysteine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q02205 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 995DD1E68331D050	FASTA	184	20,242
	10 20 30 40 50 60 MGAVLSCCRN HSGEENEALL REQQAGYGSQ GNANDEYDAE QMRLKEHEHE QKLLAREQEL 70 80 90 100 110 120 RDIVANTNDK LIDISMINNS GIVIQGTDLQ EALDKRQQEE GGDSREDERS AGDDNLSGHS 130 140 150 160 170 180 VPSSGSAQAT THQTAPRTNT FTLLTSPDSA KISKEQLKKL HSNILNEIFS QSQVNKPGPL TVPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing and analysis of a 24.7 kb segment encompassing centromere CEN11 of Saccharomyces cerevisiae reveals nine previously unknown open reading frames." Duesterhoeft A., Philippsen P. Yeast 8:749-759(1992) [PubMed: 1441752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The TOR and EGO protein complexes orchestrate microautophagy in yeast." Dubouloz F., Deloche O., Wanke V., Cameroni E., De Virgilio C. Mol. Cell 19:15-26(2005) [PubMed: 15989961] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE EGO COMPLEX, SUBCELLULAR LOCATION.
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Global analysis of protein palmitoylation in yeast." Roth A.F., Wan J., Bailey A.O., Sun B., Kuchar J.A., Green W.N., Phinney B.S., Yates J.R. III, Davis N.G. Cell 125:1003-1013(2006) [PubMed: 16751107] [Abstract] Cited for: PALMITOYLATION.
[8]	"A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast." Gao M., Kaiser C.A. Nat. Cell Biol. 8:657-667(2006) [PubMed: 16732272] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE GSE COMPLEX, MYRISTOYLATION.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146 AND SER-149, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X65124 Genomic DNA. Translation: CAA46245.1. Z28232 Genomic DNA. Translation: CAA82077.1. BK006944 Genomic DNA. Translation: DAA09163.1.
PIR	S25815.
RefSeq	NP_012932.1. NM_001179797.1.
3D structure databases
ProteinModelPortal	Q02205.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1922N.
IntAct	Q02205. 20 interactions.
MINT	MINT-400825.
STRING	Q02205.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR007W; YKR007W; YKR007W.
GeneID	853876.
KEGG	sce:YKR007W.
NMPDR	fig|4932.3.peg.3918.
Organism-specific databases
CYGD	YKR007w.
SGD	S000001715. MEH1.
Phylogenomic databases
eggNOG	fuNOG11987.
HOGENOM	HBG202355.
OrthoDB	EOG4SN4ZK.
Gene expression databases
ArrayExpress	Q02205.
Genevestigator	Q02205.
GermOnline	YKR007W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	975157.

Entry information

Entry name

MEH1_YEAST

Accession

Primary (citable) accession number: Q02205
Secondary accession number(s): D6VX73

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program