Protein MEH1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot Q02205 (MEH1_YEAST)

Last modified December 15, 2009. Version 65. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Protein MEH1
Alternative name(s):
    GSE complex subunit 2
    EGO complex subunit 1

Gene names

Name:	MEH1
Synonyms:	EGO1, GSE2
Ordered Locus Names:	YKR007W
ORF Names:	YK106

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Component of the EGO complex, a complex involved in the regulation of microautophagy. Ref.4 Ref.7
Subunit structure	Component of the GSE complex composed of GTR1, GTR2, SLM4, MEH1 and LTV1. Component of the EGO complex, at least composed of GTR2, SLM4 and MEH1.
Subcellular location	Vacuole membrane Ref.4.
Miscellaneous	Present with 2740 molecules/cell in log phase SD medium. Ref.3

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Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Membrane Vacuole
Domain	Coiled coil
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	microautophagy Ref.4 Inferred from mutant phenotype. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW vacuolar acidification Inferred from mutant phenotype. Source: SGD
Cellular component	EGO complex Ref.4 Inferred from physical interaction. Source: SGD GSE complex Ref.7 Inferred from direct assay. Source: SGD fungal-type vacuole membrane Ref.4 Inferred from direct assay. Source: SGD late endosome membrane Ref.7 Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-27062,EBI-27062
FTH1	P38310	1	EBI-27062,EBI-20959
GTR1	Q00582	1	EBI-27062,EBI-7954
GTR2	P53290	2	EBI-27062,EBI-7962
PIB2	P53191	1	EBI-27062,EBI-13400
PMC1	P38929	1	EBI-27062,EBI-3097
SLM4	P38247	2	EBI-27062,EBI-21507
SNA3	P14359	1	EBI-27062,EBI-26122
SNA4	Q07549	1	EBI-27062,EBI-22078
VAC8	P39968	1	EBI-27062,EBI-20212
VMA13	P41807	1	EBI-27062,EBI-20281
VMA4	P22203	1	EBI-27062,EBI-20268
VMA7	P39111	1	EBI-27062,EBI-20272
VMA8	P32610	1	EBI-27062,EBI-20264
VPH1	P32563	1	EBI-27062,EBI-20455
VPS35	P34110	1	EBI-27062,EBI-20415
YBT1	P32386	1	EBI-27062,EBI-21511

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Probable

Chain

2 – 184

183

Protein MEH1

PRO_0000203193

Regions

Coiled coil

30 – 71

Potential

Amino acid modifications

Modified residue

117

Phosphoserine Ref.9

Modified residue

146

Phosphoserine Ref.5 Ref.8 Ref.10

Modified residue

149

Phosphoserine Ref.8

Lipidation

N-myristoyl glycine Probable

Lipidation

S-palmitoyl cysteine Probable

Lipidation

S-palmitoyl cysteine Probable

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Sequences

Sequence

Length

Mass (Da)

Tools

Q02205-1 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 995DD1E68331D050
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FASTA

184

20,242

        10         20         30         40         50         60 
MGAVLSCCRN HSGEENEALL REQQAGYGSQ GNANDEYDAE QMRLKEHEHE QKLLAREQEL 

        70         80         90        100        110        120 
RDIVANTNDK LIDISMINNS GIVIQGTDLQ EALDKRQQEE GGDSREDERS AGDDNLSGHS 

       130        140        150        160        170        180 
VPSSGSAQAT THQTAPRTNT FTLLTSPDSA KISKEQLKKL HSNILNEIFS QSQVNKPGPL 


TVPF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing and analysis of a 24.7 kb segment encompassing centromere CEN11 of Saccharomyces cerevisiae reveals nine previously unknown open reading frames." Duesterhoeft A., Philippsen P. Yeast 8:749-759(1992) [PubMed: 1441752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"The TOR and EGO protein complexes orchestrate microautophagy in yeast." Dubouloz F., Deloche O., Wanke V., Cameroni E., De Virgilio C. Mol. Cell 19:15-26(2005) [PubMed: 15989961] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE EGO COMPLEX, SUBCELLULAR LOCATION.
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, MASS SPECTROMETRY.
[6]	"Global analysis of protein palmitoylation in yeast." Roth A.F., Wan J., Bailey A.O., Sun B., Kuchar J.A., Green W.N., Phinney B.S., Yates J.R. III, Davis N.G. Cell 125:1003-1013(2006) [PubMed: 16751107] [Abstract] Cited for: PALMITOYLATION.
[7]	"A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast." Gao M., Kaiser C.A. Nat. Cell Biol. 8:657-667(2006) [PubMed: 16732272] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE GSE COMPLEX, MYRISTOYLATION.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146 AND SER-149, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X65124 Genomic DNA. Translation: CAA46245.1. Z28232 Genomic DNA. Translation: CAA82077.1.
PIR	S25815.
RefSeq	NP_012932.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1922N.
IntAct	Q02205. 21 interactions.
STRING	Q02205.
Genome annotation databases
Ensembl	YKR007W; YKR007W; YKR007W; Saccharomyces cerevisiae. [Genome view]
GeneID	853876.
KEGG	sce:YKR007W.
NMPDR	fig\|4932.3.peg.3918.
Organism-specific databases
CYGD	YKR007w.
SGD	S000001715. MEH1.
Phylogenomic databases
HOGENOM	HBG202355.
OrthoDB	EOG9F4TTX.
Gene expression databases
ArrayExpress	Q02205.
Genevestigator	Q02205.
GermOnline	YKR007W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	975157.

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Entry information

Entry name

MEH1_YEAST

Accession

Primary (citable) accession number: Q02205

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	December 15, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents