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Q02199

- NUP49_YEAST

UniProt

Q02199 - NUP49_YEAST

Protein

Nucleoporin NUP49/NSP49

Gene

NUP49

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP49 plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, and pre-ribosome transport.8 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. nucleocytoplasmic transporter activity Source: SGD
    3. protein binding Source: IntAct
    4. structural constituent of nuclear pore Source: SGD

    GO - Biological processi

    1. poly(A)+ mRNA export from nucleus Source: SGD
    2. protein import into nucleus Source: SGD
    3. ribosomal large subunit export from nucleus Source: SGD
    4. tRNA export from nucleus Source: SGD

    Keywords - Biological processi

    mRNA transport, Protein transport, Translocation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30660-MONOMER.

    Protein family/group databases

    TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoporin NUP49/NSP49
    Alternative name(s):
    Nuclear pore protein NUP49/NSP49
    Gene namesi
    Name:NUP49
    Synonyms:NSP49
    Ordered Locus Names:YGL172W
    ORF Names:G1648
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL172w.
    SGDiS000003140. NUP49.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear membrane Source: UniProtKB-SubCell
    2. nuclear pore Source: SGD
    3. nuclear pore central transport channel Source: SGD

    Keywords - Cellular componenti

    Membrane, Nuclear pore complex, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 472472Nucleoporin NUP49/NSP49PRO_0000204867Add
    BLAST

    Proteomic databases

    MaxQBiQ02199.
    PaxDbiQ02199.
    PeptideAtlasiQ02199.

    Expressioni

    Gene expression databases

    GenevestigatoriQ02199.

    Interactioni

    Subunit structurei

    The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP49 is part of the NUP57 subcomplex (NIC96, NSP1, NUP49, NUP57) interacting with NUP57. Interacts through its FG repeats with karyopherins.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-12315,EBI-12315
    KAP95Q061422EBI-12315,EBI-9145
    NIC96P340773EBI-12315,EBI-12056
    NUP57P488377EBI-12315,EBI-12324

    Protein-protein interaction databases

    BioGridi33081. 53 interactions.
    DIPiDIP-709N.
    IntActiQ02199. 23 interactions.
    MINTiMINT-398162.
    STRINGi4932.YGL172W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02199.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati2 – 32FG 1
    Repeati14 – 174GLFG 1
    Repeati33 – 342FG 2
    Repeati48 – 514GLFG 2
    Repeati65 – 662FG 3
    Repeati77 – 782FG 4
    Repeati86 – 894GLFG 3
    Repeati101 – 1044GLFG 4
    Repeati113 – 1164SLFG 1
    Repeati125 – 1284GLFG 5
    Repeati148 – 1514GLFG 6
    Repeati159 – 1624SLFG 2
    Repeati175 – 1784GLFG 7; approximate
    Repeati185 – 1884SLFG 3
    Repeati199 – 2024GLFG 8
    Repeati210 – 2134SLFG 4
    Repeati233 – 2364GLFG 9

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi13 – 207195Gly-richAdd
    BLAST
    Compositional biasi67 – 737Poly-Gln
    Compositional biasi90 – 226137Asn-richAdd
    BLAST
    Compositional biasi216 – 22611Poly-AsnAdd
    BLAST
    Compositional biasi238 – 28952Gln-richAdd
    BLAST
    Compositional biasi238 – 24912Poly-GlnAdd
    BLAST

    Domaini

    Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side: GLFG repeats are especially abundant in NUPs in the central region (lacking a charged environment but are enriched in Ser, Thr, Gln, and Asn).

    Sequence similaritiesi

    Belongs to the nucleoporin GLFG family.Curated

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiNOG269639.
    GeneTreeiENSGT00730000113226.
    HOGENOMiHOG000246824.
    KOiK14307.
    OMAiFMELCET.
    OrthoDBiEOG78PVKQ.

    Family and domain databases

    InterProiIPR025574. Nucleoporin_FG_rpt.
    [Graphical view]
    PfamiPF13634. Nucleoporin_FG. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02199-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFGLNKASST PAGGLFGQAS GASTGNANTG FSFGGTQTGQ NTGPSTGGLF    50
    GAKPAGSTGG LGASFGQQQQ QSQTNAFGGS ATTGGGLFGN KPNNTANTGG 100
    GLFGANSNSN SGSLFGSNNA QTSRGLFGNN NTNNINNSSS GMNNASAGLF 150
    GSKPAGGTSL FGNTSTSSAP AQNQGMFGAK PAGTSLFGNN AGNTTTGGGL 200
    FGSKPTGATS LFGSSNNNNN NNNSNNIMSA SGGLFGNQQQ QLQQQPQMQC 250
    ALQNLSQLPI TPMTRISELP PQIRQEIEQL DQYIQKQVQI SHHLKADTID 300
    HDELIDSIPR DVAYLLKSES ATSQYLKQDL KKISSFKSLI DEDLLDTQTF 350
    SVLLQQLLTP GSKISSNDLD KFFQKKIHLY EKKLEDYCRI LSDIETAVNG 400
    IDTDLFGAPN NPNSTAITAD LGSSEAENLL QLKTGLAAIV STVIEEFTLF 450
    MDIAERIAVL HQKTKTLASL SI 472
    Length:472
    Mass (Da):49,142
    Last modified:July 1, 1993 - v1
    Checksum:i0CA7516FF5753CA3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68109 Genomic DNA. Translation: CAA48229.1.
    Z15040 Genomic DNA. Translation: CAA78758.1.
    X84705 Genomic DNA. Translation: CAA59181.1.
    Z72694 Genomic DNA. Translation: CAA96884.1.
    BK006941 Genomic DNA. Translation: DAA07941.1.
    PIRiS28026.
    RefSeqiNP_011343.1. NM_001181037.1.

    Genome annotation databases

    EnsemblFungiiYGL172W; YGL172W; YGL172W.
    GeneIDi852703.
    KEGGisce:YGL172W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68109 Genomic DNA. Translation: CAA48229.1 .
    Z15040 Genomic DNA. Translation: CAA78758.1 .
    X84705 Genomic DNA. Translation: CAA59181.1 .
    Z72694 Genomic DNA. Translation: CAA96884.1 .
    BK006941 Genomic DNA. Translation: DAA07941.1 .
    PIRi S28026.
    RefSeqi NP_011343.1. NM_001181037.1.

    3D structure databases

    ProteinModelPortali Q02199.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33081. 53 interactions.
    DIPi DIP-709N.
    IntActi Q02199. 23 interactions.
    MINTi MINT-398162.
    STRINGi 4932.YGL172W.

    Protein family/group databases

    TCDBi 1.I.1.1.1. the nuclear pore complex (npc) family.

    Proteomic databases

    MaxQBi Q02199.
    PaxDbi Q02199.
    PeptideAtlasi Q02199.

    Protocols and materials databases

    DNASUi 852703.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL172W ; YGL172W ; YGL172W .
    GeneIDi 852703.
    KEGGi sce:YGL172W.

    Organism-specific databases

    CYGDi YGL172w.
    SGDi S000003140. NUP49.

    Phylogenomic databases

    eggNOGi NOG269639.
    GeneTreei ENSGT00730000113226.
    HOGENOMi HOG000246824.
    KOi K14307.
    OMAi FMELCET.
    OrthoDBi EOG78PVKQ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30660-MONOMER.

    Miscellaneous databases

    NextBioi 972053.
    PROi Q02199.

    Gene expression databases

    Genevestigatori Q02199.

    Family and domain databases

    InterProi IPR025574. Nucleoporin_FG_rpt.
    [Graphical view ]
    Pfami PF13634. Nucleoporin_FG. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1."
      Wimmer C., Doye V., Grandi P., Nehrbass U., Hurt E.C.
      EMBO J. 11:5051-5061(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A new family of yeast nuclear pore complex proteins."
      Wente S.R., Rout M.P., Blobel G.
      J. Cell Biol. 119:705-723(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes."
      Bertani I., Coglievina M., Zaccaria P., Klima R., Bruschi C.V.
      Yeast 11:1187-1194(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution."
      Doye V., Wepf R., Hurt E.C.
      EMBO J. 13:6062-6075(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN MRNA EXPORT.
    7. "Yeast nucleoporin mutants are defective in pre-tRNA splicing."
      Sharma K., Fabre E., Tekotte H., Hurt E.C., Tollervey D.
      Mol. Cell. Biol. 16:294-301(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TRNA EXPORT.
    8. "In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p."
      Schlaich N.L., Haener M., Lustig A., Aebi U., Hurt E.C.
      Mol. Biol. Cell 8:33-46(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, THE NUP57 SUBCOMPLEX.
    9. "A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants."
      Hurt E.C., Hannus S., Schmelzl B., Lau D.M., Tollervey D., Simos G.
      J. Cell Biol. 144:389-401(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PRE-RIBOSOME EXPORT.
    10. "The yeast nuclear pore complex: composition, architecture, and transport mechanism."
      Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
      J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
    11. "Proteomic analysis of nucleoporin interacting proteins."
      Allen N.P., Huang L., Burlingame A., Rexach M.
      J. Biol. Chem. 276:29268-29274(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, NUCLEOPORIN INTERACTING PROTEINS.
    12. "The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport."
      Bailer S.M., Balduf C., Hurt E.C.
      Mol. Cell. Biol. 21:7944-7955(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, NPC ASSEMBLY.
    13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    14. "Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded."
      Denning D.P., Patel S.S., Uversky V., Fink A.L., Rexach M.
      Proc. Natl. Acad. Sci. U.S.A. 100:2450-2455(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, FG REPEAT STRUCTURE.
    15. "Minimal nuclear pore complexes define FG repeat domains essential for transport."
      Strawn L.A., Shen T.X., Shulga N., Goldfarb D.S., Wente S.R.
      Nat. Cell Biol. 6:197-206(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, FG REPEATS IN NPC TRANSPORT.
    16. "Peering through the pore: nuclear pore complex structure, assembly, and function."
      Suntharalingam M., Wente S.R.
      Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiNUP49_YEAST
    AccessioniPrimary (citable) accession number: Q02199
    Secondary accession number(s): D6VTY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4760 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3