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Q02199

- NUP49_YEAST

UniProt

Q02199 - NUP49_YEAST

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Protein

Nucleoporin NUP49/NSP49

Gene

NUP49

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP49 plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, and pre-ribosome transport.8 Publications

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. nucleocytoplasmic transporter activity Source: SGD
  3. structural constituent of nuclear pore Source: SGD

GO - Biological processi

  1. poly(A)+ mRNA export from nucleus Source: SGD
  2. protein import into nucleus Source: SGD
  3. ribosomal large subunit export from nucleus Source: SGD
  4. tRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30660-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP49/NSP49
Alternative name(s):
Nuclear pore protein NUP49/NSP49
Gene namesi
Name:NUP49
Synonyms:NSP49
Ordered Locus Names:YGL172W
ORF Names:G1648
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL172w.
SGDiS000003140. NUP49.

Subcellular locationi

GO - Cellular componenti

  1. nuclear pore Source: SGD
  2. nuclear pore central transport channel Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Nucleoporin NUP49/NSP49PRO_0000204867Add
BLAST

Proteomic databases

MaxQBiQ02199.
PaxDbiQ02199.
PeptideAtlasiQ02199.

Expressioni

Gene expression databases

GenevestigatoriQ02199.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP49 is part of the NUP57 subcomplex (NIC96, NSP1, NUP49, NUP57) interacting with NUP57. Interacts through its FG repeats with karyopherins.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-12315,EBI-12315
KAP95Q061422EBI-12315,EBI-9145
NIC96P340773EBI-12315,EBI-12056
NUP57P488377EBI-12315,EBI-12324

Protein-protein interaction databases

BioGridi33081. 54 interactions.
DIPiDIP-709N.
IntActiQ02199. 23 interactions.
MINTiMINT-398162.
STRINGi4932.YGL172W.

Structurei

3D structure databases

ProteinModelPortaliQ02199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati2 – 32FG 1
Repeati14 – 174GLFG 1
Repeati33 – 342FG 2
Repeati48 – 514GLFG 2
Repeati65 – 662FG 3
Repeati77 – 782FG 4
Repeati86 – 894GLFG 3
Repeati101 – 1044GLFG 4
Repeati113 – 1164SLFG 1
Repeati125 – 1284GLFG 5
Repeati148 – 1514GLFG 6
Repeati159 – 1624SLFG 2
Repeati175 – 1784GLFG 7; approximate
Repeati185 – 1884SLFG 3
Repeati199 – 2024GLFG 8
Repeati210 – 2134SLFG 4
Repeati233 – 2364GLFG 9

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 207195Gly-richAdd
BLAST
Compositional biasi67 – 737Poly-Gln
Compositional biasi90 – 226137Asn-richAdd
BLAST
Compositional biasi216 – 22611Poly-AsnAdd
BLAST
Compositional biasi238 – 28952Gln-richAdd
BLAST
Compositional biasi238 – 24912Poly-GlnAdd
BLAST

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side: GLFG repeats are especially abundant in NUPs in the central region (lacking a charged environment but are enriched in Ser, Thr, Gln, and Asn).

Sequence similaritiesi

Belongs to the nucleoporin GLFG family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG269639.
GeneTreeiENSGT00730000113226.
HOGENOMiHOG000246824.
InParanoidiQ02199.
KOiK14307.
OMAiFMELCET.
OrthoDBiEOG78PVKQ.

Family and domain databases

InterProiIPR025574. Nucleoporin_FG_rpt.
[Graphical view]
PfamiPF13634. Nucleoporin_FG. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02199-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFGLNKASST PAGGLFGQAS GASTGNANTG FSFGGTQTGQ NTGPSTGGLF
60 70 80 90 100
GAKPAGSTGG LGASFGQQQQ QSQTNAFGGS ATTGGGLFGN KPNNTANTGG
110 120 130 140 150
GLFGANSNSN SGSLFGSNNA QTSRGLFGNN NTNNINNSSS GMNNASAGLF
160 170 180 190 200
GSKPAGGTSL FGNTSTSSAP AQNQGMFGAK PAGTSLFGNN AGNTTTGGGL
210 220 230 240 250
FGSKPTGATS LFGSSNNNNN NNNSNNIMSA SGGLFGNQQQ QLQQQPQMQC
260 270 280 290 300
ALQNLSQLPI TPMTRISELP PQIRQEIEQL DQYIQKQVQI SHHLKADTID
310 320 330 340 350
HDELIDSIPR DVAYLLKSES ATSQYLKQDL KKISSFKSLI DEDLLDTQTF
360 370 380 390 400
SVLLQQLLTP GSKISSNDLD KFFQKKIHLY EKKLEDYCRI LSDIETAVNG
410 420 430 440 450
IDTDLFGAPN NPNSTAITAD LGSSEAENLL QLKTGLAAIV STVIEEFTLF
460 470
MDIAERIAVL HQKTKTLASL SI
Length:472
Mass (Da):49,142
Last modified:July 1, 1993 - v1
Checksum:i0CA7516FF5753CA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68109 Genomic DNA. Translation: CAA48229.1.
Z15040 Genomic DNA. Translation: CAA78758.1.
X84705 Genomic DNA. Translation: CAA59181.1.
Z72694 Genomic DNA. Translation: CAA96884.1.
BK006941 Genomic DNA. Translation: DAA07941.1.
PIRiS28026.
RefSeqiNP_011343.1. NM_001181037.1.

Genome annotation databases

EnsemblFungiiYGL172W; YGL172W; YGL172W.
GeneIDi852703.
KEGGisce:YGL172W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68109 Genomic DNA. Translation: CAA48229.1 .
Z15040 Genomic DNA. Translation: CAA78758.1 .
X84705 Genomic DNA. Translation: CAA59181.1 .
Z72694 Genomic DNA. Translation: CAA96884.1 .
BK006941 Genomic DNA. Translation: DAA07941.1 .
PIRi S28026.
RefSeqi NP_011343.1. NM_001181037.1.

3D structure databases

ProteinModelPortali Q02199.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33081. 54 interactions.
DIPi DIP-709N.
IntActi Q02199. 23 interactions.
MINTi MINT-398162.
STRINGi 4932.YGL172W.

Protein family/group databases

TCDBi 1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

MaxQBi Q02199.
PaxDbi Q02199.
PeptideAtlasi Q02199.

Protocols and materials databases

DNASUi 852703.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL172W ; YGL172W ; YGL172W .
GeneIDi 852703.
KEGGi sce:YGL172W.

Organism-specific databases

CYGDi YGL172w.
SGDi S000003140. NUP49.

Phylogenomic databases

eggNOGi NOG269639.
GeneTreei ENSGT00730000113226.
HOGENOMi HOG000246824.
InParanoidi Q02199.
KOi K14307.
OMAi FMELCET.
OrthoDBi EOG78PVKQ.

Enzyme and pathway databases

BioCyci YEAST:G3O-30660-MONOMER.

Miscellaneous databases

NextBioi 972053.
PROi Q02199.

Gene expression databases

Genevestigatori Q02199.

Family and domain databases

InterProi IPR025574. Nucleoporin_FG_rpt.
[Graphical view ]
Pfami PF13634. Nucleoporin_FG. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1."
    Wimmer C., Doye V., Grandi P., Nehrbass U., Hurt E.C.
    EMBO J. 11:5051-5061(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A new family of yeast nuclear pore complex proteins."
    Wente S.R., Rout M.P., Blobel G.
    J. Cell Biol. 119:705-723(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes."
    Bertani I., Coglievina M., Zaccaria P., Klima R., Bruschi C.V.
    Yeast 11:1187-1194(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution."
    Doye V., Wepf R., Hurt E.C.
    EMBO J. 13:6062-6075(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MRNA EXPORT.
  7. "Yeast nucleoporin mutants are defective in pre-tRNA splicing."
    Sharma K., Fabre E., Tekotte H., Hurt E.C., Tollervey D.
    Mol. Cell. Biol. 16:294-301(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRNA EXPORT.
  8. "In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p."
    Schlaich N.L., Haener M., Lustig A., Aebi U., Hurt E.C.
    Mol. Biol. Cell 8:33-46(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, THE NUP57 SUBCOMPLEX.
  9. "A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants."
    Hurt E.C., Hannus S., Schmelzl B., Lau D.M., Tollervey D., Simos G.
    J. Cell Biol. 144:389-401(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PRE-RIBOSOME EXPORT.
  10. "The yeast nuclear pore complex: composition, architecture, and transport mechanism."
    Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
    J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
  11. "Proteomic analysis of nucleoporin interacting proteins."
    Allen N.P., Huang L., Burlingame A., Rexach M.
    J. Biol. Chem. 276:29268-29274(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, NUCLEOPORIN INTERACTING PROTEINS.
  12. "The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport."
    Bailer S.M., Balduf C., Hurt E.C.
    Mol. Cell. Biol. 21:7944-7955(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, NPC ASSEMBLY.
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded."
    Denning D.P., Patel S.S., Uversky V., Fink A.L., Rexach M.
    Proc. Natl. Acad. Sci. U.S.A. 100:2450-2455(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, FG REPEAT STRUCTURE.
  15. "Minimal nuclear pore complexes define FG repeat domains essential for transport."
    Strawn L.A., Shen T.X., Shulga N., Goldfarb D.S., Wente S.R.
    Nat. Cell Biol. 6:197-206(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, FG REPEATS IN NPC TRANSPORT.
  16. "Peering through the pore: nuclear pore complex structure, assembly, and function."
    Suntharalingam M., Wente S.R.
    Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiNUP49_YEAST
AccessioniPrimary (citable) accession number: Q02199
Secondary accession number(s): D6VTY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4760 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3