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Q02199 (NUP49_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoporin NUP49/NSP49
Alternative name(s):
Nuclear pore protein NUP49/NSP49
Gene names
Name:NUP49
Synonyms:NSP49
Ordered Locus Names:YGL172W
ORF Names:G1648
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP49 plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, and pre-ribosome transport. Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12 Ref.14 Ref.15

Subunit structure

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP49 is part of the NUP57 subcomplex (NIC96, NSP1, NUP49, NUP57) interacting with NUP57. Interacts through its FG repeats with karyopherins. Ref.10

Subcellular location

Nucleusnuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Symmetric distribution.

Domain

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side: GLFG repeats are especially abundant in NUPs in the central region (lacking a charged environment but are enriched in Ser, Thr, Gln, and Asn).

Miscellaneous

Present with 4760 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the nucleoporin GLFG family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472Nucleoporin NUP49/NSP49
PRO_0000204867

Regions

Repeat2 – 32FG 1
Repeat14 – 174GLFG 1
Repeat33 – 342FG 2
Repeat48 – 514GLFG 2
Repeat65 – 662FG 3
Repeat77 – 782FG 4
Repeat86 – 894GLFG 3
Repeat101 – 1044GLFG 4
Repeat113 – 1164SLFG 1
Repeat125 – 1284GLFG 5
Repeat148 – 1514GLFG 6
Repeat159 – 1624SLFG 2
Repeat175 – 1784GLFG 7; approximate
Repeat185 – 1884SLFG 3
Repeat199 – 2024GLFG 8
Repeat210 – 2134SLFG 4
Repeat233 – 2364GLFG 9
Compositional bias13 – 207195Gly-rich
Compositional bias67 – 737Poly-Gln
Compositional bias90 – 226137Asn-rich
Compositional bias216 – 22611Poly-Asn
Compositional bias238 – 28952Gln-rich
Compositional bias238 – 24912Poly-Gln

Sequences

Sequence LengthMass (Da)Tools
Q02199 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 0CA7516FF5753CA3

FASTA47249,142
        10         20         30         40         50         60 
MFGLNKASST PAGGLFGQAS GASTGNANTG FSFGGTQTGQ NTGPSTGGLF GAKPAGSTGG 

        70         80         90        100        110        120 
LGASFGQQQQ QSQTNAFGGS ATTGGGLFGN KPNNTANTGG GLFGANSNSN SGSLFGSNNA 

       130        140        150        160        170        180 
QTSRGLFGNN NTNNINNSSS GMNNASAGLF GSKPAGGTSL FGNTSTSSAP AQNQGMFGAK 

       190        200        210        220        230        240 
PAGTSLFGNN AGNTTTGGGL FGSKPTGATS LFGSSNNNNN NNNSNNIMSA SGGLFGNQQQ 

       250        260        270        280        290        300 
QLQQQPQMQC ALQNLSQLPI TPMTRISELP PQIRQEIEQL DQYIQKQVQI SHHLKADTID 

       310        320        330        340        350        360 
HDELIDSIPR DVAYLLKSES ATSQYLKQDL KKISSFKSLI DEDLLDTQTF SVLLQQLLTP 

       370        380        390        400        410        420 
GSKISSNDLD KFFQKKIHLY EKKLEDYCRI LSDIETAVNG IDTDLFGAPN NPNSTAITAD 

       430        440        450        460        470 
LGSSEAENLL QLKTGLAAIV STVIEEFTLF MDIAERIAVL HQKTKTLASL SI 

« Hide

References

« Hide 'large scale' references
[1]"A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1."
Wimmer C., Doye V., Grandi P., Nehrbass U., Hurt E.C.
EMBO J. 11:5051-5061(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A new family of yeast nuclear pore complex proteins."
Wente S.R., Rout M.P., Blobel G.
J. Cell Biol. 119:705-723(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes."
Bertani I., Coglievina M., Zaccaria P., Klima R., Bruschi C.V.
Yeast 11:1187-1194(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution."
Doye V., Wepf R., Hurt E.C.
EMBO J. 13:6062-6075(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN MRNA EXPORT.
[7]"Yeast nucleoporin mutants are defective in pre-tRNA splicing."
Sharma K., Fabre E., Tekotte H., Hurt E.C., Tollervey D.
Mol. Cell. Biol. 16:294-301(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN TRNA EXPORT.
[8]"In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p."
Schlaich N.L., Haener M., Lustig A., Aebi U., Hurt E.C.
Mol. Biol. Cell 8:33-46(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, THE NUP57 SUBCOMPLEX.
[9]"A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants."
Hurt E.C., Hannus S., Schmelzl B., Lau D.M., Tollervey D., Simos G.
J. Cell Biol. 144:389-401(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PRE-RIBOSOME EXPORT.
[10]"The yeast nuclear pore complex: composition, architecture, and transport mechanism."
Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
[11]"Proteomic analysis of nucleoporin interacting proteins."
Allen N.P., Huang L., Burlingame A., Rexach M.
J. Biol. Chem. 276:29268-29274(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, NUCLEOPORIN INTERACTING PROTEINS.
[12]"The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport."
Bailer S.M., Balduf C., Hurt E.C.
Mol. Cell. Biol. 21:7944-7955(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, NPC ASSEMBLY.
[13]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[14]"Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded."
Denning D.P., Patel S.S., Uversky V., Fink A.L., Rexach M.
Proc. Natl. Acad. Sci. U.S.A. 100:2450-2455(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, FG REPEAT STRUCTURE.
[15]"Minimal nuclear pore complexes define FG repeat domains essential for transport."
Strawn L.A., Shen T.X., Shulga N., Goldfarb D.S., Wente S.R.
Nat. Cell Biol. 6:197-206(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, FG REPEATS IN NPC TRANSPORT.
[16]"Peering through the pore: nuclear pore complex structure, assembly, and function."
Suntharalingam M., Wente S.R.
Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68109 Genomic DNA. Translation: CAA48229.1.
Z15040 Genomic DNA. Translation: CAA78758.1.
X84705 Genomic DNA. Translation: CAA59181.1.
Z72694 Genomic DNA. Translation: CAA96884.1.
BK006941 Genomic DNA. Translation: DAA07941.1.
PIRS28026.
RefSeqNP_011343.1. NM_001181037.1.

3D structure databases

ProteinModelPortalQ02199.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33081. 53 interactions.
DIPDIP-709N.
IntActQ02199. 23 interactions.
MINTMINT-398162.
STRING4932.YGL172W.

Protein family/group databases

TCDB1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

PaxDbQ02199.
PeptideAtlasQ02199.

Protocols and materials databases

DNASU852703.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL172W; YGL172W; YGL172W.
GeneID852703.
KEGGsce:YGL172W.

Organism-specific databases

CYGDYGL172w.
SGDS000003140. NUP49.

Phylogenomic databases

eggNOGNOG269639.
GeneTreeENSGT00730000113226.
HOGENOMHOG000246824.
KOK14307.
OMATKQALKF.
OrthoDBEOG78PVKQ.

Enzyme and pathway databases

BioCycYEAST:G3O-30660-MONOMER.

Gene expression databases

GenevestigatorQ02199.

Family and domain databases

InterProIPR025574. Nucleoporin_FG_rpt.
[Graphical view]
PfamPF13634. Nucleoporin_FG. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio972053.
PROQ02199.

Entry information

Entry nameNUP49_YEAST
AccessionPrimary (citable) accession number: Q02199
Secondary accession number(s): D6VTY0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families