Reviewed, UniProtKB/Swiss-Prot Q02198 (MORA_PSEPU)

Names and origin

Protein names

Recommended name:
    Morphine 6-dehydrogenase
    EC=1.1.1.218
Alternative name(s):
    Naloxone reductase

Gene names

Name:

morA

Encoded on

Plasmid pMDH7.2

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Oxidizes only the C-6 hydroxy group of morphine and codeine.
Catalytic activity	Morphine + NAD(P)+ = morphinone + NAD(P)H.
Pathway	Alkaloid degradation; codeine degradation. Alkaloid degradation; morphine degradation.
Subunit structure	Monomer.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	morphine 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.3
	Chain	2 – 296	295	Morphine 6-dehydrogenase		PRO_0000124675
Regions
	Nucleotide binding	13 – 22	10	NADP Potential
Sites
	Active site	52	1	Proton donor By similarity
	Binding site	110	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02198-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: CC16A1DEAE804AA7	FASTA	296	32,124
	10 20 30 40 50 60 MAGKSPLINL NNGVKMPALG LGVFAASAEE TASAIASAIS SGYRLIDTAR SYNNEAQVGE 70 80 90 100 110 120 GIRNSGVDRA EMFVTTKLFN CDYGYERALR AFDESLGRLG LDYVDLYLLH WPTKDWNATI 130 140 150 160 170 180 QSWKAAEKIL GDGRARAIGV CNFLEDQLDE LIAASDVVPA VNQIELHPYF AQKPLLAKNR 190 200 210 220 230 240 ALGIVTEAWS PIGGAINDGD GDNHGGRKHP LTDPVITTIA EAHGRSAAQV ILRWHFQNDV 250 260 270 280 290 VAIPKSVNPE RIAKNIDVFD FALSDAEMAQ LDELDTGVRI GPDPRDVDTS SFAEFV

References

[1]	"Nucleotide sequence and over-expression of morphine dehydrogenase, a plasmid-encoded gene from Pseudomonas putida M10." Willey D.L., Caswell D.A., Lowe C.R., Bruce N.C. Biochem. J. 290:539-544(1993) [PubMed: 8452544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-26. Strain: M10.
[2]	Bruce N.C. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Microbial degradation of the morphine alkaloids. Purification and characterization of morphine dehydrogenase from Pseudomonas putida M10." Bruce N.C., Wilmot C.J., Jordan K.N., Stephens L.D.G., Lowe C.R. Biochem. J. 274:875-880(1991) [PubMed: 2012614] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26, CHARACTERIZATION. Strain: M10.
[4]	"Bacterial morphine dehydrogenase further defines a distinct superfamily of oxidoreductases with diverse functional activities." Bruce N.C., Willey D.L., Coulson A.F.W., Jeffery J. Biochem. J. 299:805-811(1994) [PubMed: 8192670] [Abstract] Cited for: SIMILARITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M94775 Genomic DNA. Translation: AAB17356.1.
3D structure databases
HSSP	HSSP built from PDB template 1HW6 based on UniProtKB P06632.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.218. 403.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. False negative. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MORA_PSEPU

Accession

Primary (citable) accession number: Q02198

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)