Reviewed, UniProtKB/Swiss-Prot Q02197 (MAK10_YEAST)

Names and origin

Protein names

Recommended name:
    N-terminal acetyltransferase C complex subunit MAK10
      Short name=NatC complex subunit MAK10
Alternative name(s):
    L-A virus GAG protein N-acetyltransferase subunit MAK10
    Glucose repressible protein MAK10
    Maintenance of killer protein 10

Gene names

Name:	MAK10
Ordered Locus Names:	YEL053C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the NatC N-terminal acetyltransferase, which catalyzes acetylation of the N-terminus Met of L-A virus Gag protein. MAK10 has a role in the propagation of L-A and M viruses, perhaps in the viral assembly. It is apparently directly needed for optimum respiration. Ref.3
Subunit structure	Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of MAK3, MAK10 and MAK31.
Subcellular location	Cytoplasm.
Induction	Glucose-repressed.
Miscellaneous	Present with 1970 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the MAK10 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-terminal protein amino acid acetylation Ref.3 Inferred from mutant phenotype. Source: SGD
Cellular component	NatC complex Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MAK3	Q03503	3	EBI-10924,EBI-10388

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 733	733	N-terminal acetyltransferase C complex subunit MAK10		PRO_0000084553

Sequences

	Sequence		Length	Mass (Da)
	Q02197-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 11148C1FC6BB0931	FASTA	733	84,302
	10 20 30 40 50 60 MEVDSILGSL SITDDFDQLV DVTSLFDELC SKLKPEAIVK DPRFDLFEGT HSLEVNNSKL 70 80 90 100 110 120 DSSLIELTAE EIEFDVNVAY DPPLASVAAI ADRLLRCVIS WLNDYQTLPT TVLSCRYTES 130 140 150 160 170 180 LLSSLVKGTT AGSSWCTGNI LYDKVLGSCI LGVCYLTKFV QKLLSAGIVF EEEDLNFNNM 190 200 210 220 230 240 GFNTFDNLPG QDVVINSLTE SLQILEAYSD DSLHLTMLKH ILKIIICLVH LEDHLTDYST 250 260 270 280 290 300 KTSHLDELIE NANSVNGIFP QLQLSPPKGA FSTYIQKHRS NQFPPRKITK LPTDYSGFIT 310 320 330 340 350 360 LANDVKTILL VDKAESALET YQFAKFFNKL EQRHVIARIL FPLFFIRDDR TVLGKFSYTQ 370 380 390 400 410 420 FYLLHVKEFS AQTPSEFESS IGNELIQESS NMLLEWYQNC SQNTCRYRQG FNRQLILWDS 430 440 450 460 470 480 LQAQFESVNS QVYCSWTYFM KLSSMIEFSL KGFDLDIYKP FEAYSMFWYV YYLSHHLETF 490 500 510 520 530 540 LKDSQNDIES NINAIHSMNK KLKKLKAGEK KDQLRLKYRF AMDNEMEQLQ ATKQFLNYLL 550 560 570 580 590 600 KEINITKSLC LIEVFQFAIL KSFGLIDNKN STPSKFSNER LIHNLRFKPF NSIGVPELPE 610 620 630 640 650 660 YEVFQQTLKD FVIEEKGAAF DIKLERATNF IETEVRNVVS SIDEIMQGIK GGDNNGVLVT 670 680 690 700 710 720 GTRLVQELSL EYYCKLKHTS KALSVNSKVI VNTLKKNIKN KDSHEYKVEL VHTTEGWNYF 730 PIQTLRIKQD RYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MAK10, a glucose-repressible gene necessary for replication of a dsRNA virus of Saccharomyces cerevisiae, has T cell receptor alpha-subunit motifs." Lee Y.-J., Wickner R.B. Genetics 132:87-96(1992) [PubMed: 1398065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"NatC Nalpha-terminal acetyltransferase of yeast contains three subunits, Mak3p, Mak10p, and Mak31p." Polevoda B., Sherman F. J. Biol. Chem. 276:20154-20159(2001) [PubMed: 11274203] [Abstract] Cited for: IDENTIFICATION IN THE NATC COMPLEX, FUNCTION OF THE NATC COMPLEX.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M94533 Genomic RNA. Translation: AAA34749.1. U18779 Genomic DNA. Translation: AAB64989.1.
PIR	S31288.
RefSeq	NP_010861.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1428N.
IntAct	Q02197. 6 interactions.
STRING	Q02197.
Proteomic databases
PRIDE	Q02197.
Genome annotation databases
Ensembl	YEL053C; YEL053C; YEL053C; Saccharomyces cerevisiae. [Genome view]
GeneID	856657.
KEGG	sce:YEL053C.
NMPDR	fig|4932.3.peg.1911.
Organism-specific databases
CYGD	YEL053c.
SGD	S000000779. MAK10.
Phylogenomic databases
HOGENOM	Q02197.
OMA	KTFSSIG
OrthoDB	EOG95QJWC
Gene expression databases
ArrayExpress	Q02197.
Genevestigator	Q02197.
GermOnline	YEL053C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007244. NatC_a_AcTrfase. [Graphical view]
Pfam	PF04112. Mak10. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982646.

Entry information

Entry name

MAK10_YEAST

Accession

Primary (citable) accession number: Q02197

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	November 24, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)