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Protein

Adenylyl-sulfate kinase

Gene

MET14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of activated sulfate.

Catalytic activityi

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase (MET3)
  2. Adenylyl-sulfate kinase (MET14)
  3. Phosphoadenosine phosphosulfate reductase (MET16)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei105 – 1051Phosphoserine intermediateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 388ATPBy similarity

GO - Molecular functioni

  • adenylylsulfate kinase activity Source: SGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • cysteine biosynthetic process Source: UniProtKB-KW
  • hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
  • methionine biosynthetic process Source: UniProtKB-KW
  • sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Source: SGD
  • sulfur amino acid metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-383.
YEAST:YKL001C-MONOMER.
ReactomeiR-SCE-174362. Transport and synthesis of PAPS.
UniPathwayiUPA00140; UER00205.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyl-sulfate kinase (EC:2.7.1.25)
Alternative name(s):
ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenosine-5'-phosphosulfate kinase
Short name:
APS kinase
Gene namesi
Name:MET14
Ordered Locus Names:YKL001C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL001C.
SGDiS000001484. MET14.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Adenylyl-sulfate kinasePRO_0000105936Add
BLAST

Proteomic databases

MaxQBiQ02196.
PeptideAtlasiQ02196.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006842EBI-9485,EBI-4192
SKP2P428433EBI-9485,EBI-28370

Protein-protein interaction databases

BioGridi34132. 41 interactions.
DIPiDIP-1972N.
IntActiQ02196. 4 interactions.
MINTiMINT-412262.

Structurei

3D structure databases

ProteinModelPortaliQ02196.
SMRiQ02196. Positions 22-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the APS kinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000009613.
HOGENOMiHOG000228204.
InParanoidiQ02196.
KOiK00860.
OMAiCRILDGD.
OrthoDBiEOG7XPZHW.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATNITWHPN LTYDERKALR KQDGCTIWLT GLSASGKSTI ACALEQLLLQ
60 70 80 90 100
KNLSAYRLDG DNIRFGLNKD LGFSEKDRNE NIRRISEVSK LFADSCAISI
110 120 130 140 150
TSFISPYRVD RDRARELHKE AGLKFIEIFV DVPLEVAEQR DPKGLYKKAR
160 170 180 190 200
EGVIKEFTGI SAPYEAPKAP ELHLRTDQKT VEECATIIYE YLISEKIIRK

HL
Length:202
Mass (Da):23,060
Last modified:July 1, 1993 - v1
Checksum:i32F301FB6B2F41D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57990 Genomic DNA. Translation: CAA41055.1.
S55315 Genomic DNA. Translation: AAB19854.1.
X65124 Genomic DNA. Translation: CAA46252.1.
Z28001 Genomic DNA. Translation: CAA81833.1.
AY558261 Genomic DNA. Translation: AAS56587.1.
BK006944 Genomic DNA. Translation: DAA09156.1.
PIRiS17244.
RefSeqiNP_012925.3. NM_001179567.3.

Genome annotation databases

EnsemblFungiiYKL001C; YKL001C; YKL001C.
GeneIDi853869.
KEGGisce:YKL001C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57990 Genomic DNA. Translation: CAA41055.1.
S55315 Genomic DNA. Translation: AAB19854.1.
X65124 Genomic DNA. Translation: CAA46252.1.
Z28001 Genomic DNA. Translation: CAA81833.1.
AY558261 Genomic DNA. Translation: AAS56587.1.
BK006944 Genomic DNA. Translation: DAA09156.1.
PIRiS17244.
RefSeqiNP_012925.3. NM_001179567.3.

3D structure databases

ProteinModelPortaliQ02196.
SMRiQ02196. Positions 22-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34132. 41 interactions.
DIPiDIP-1972N.
IntActiQ02196. 4 interactions.
MINTiMINT-412262.

Proteomic databases

MaxQBiQ02196.
PeptideAtlasiQ02196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL001C; YKL001C; YKL001C.
GeneIDi853869.
KEGGisce:YKL001C.

Organism-specific databases

EuPathDBiFungiDB:YKL001C.
SGDiS000001484. MET14.

Phylogenomic databases

GeneTreeiENSGT00390000009613.
HOGENOMiHOG000228204.
InParanoidiQ02196.
KOiK00860.
OMAiCRILDGD.
OrthoDBiEOG7XPZHW.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00205.
BioCyciMetaCyc:MONOMER-383.
YEAST:YKL001C-MONOMER.
ReactomeiR-SCE-174362. Transport and synthesis of PAPS.

Miscellaneous databases

NextBioi975136.
PROiQ02196.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, nucleotide sequence, and regulation of MET14, the gene encoding the APS kinase of Saccharomyces cerevisiae."
    Korch C., Mountain H.A., Bystroem A.S.
    Mol. Gen. Genet. 229:96-108(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204510 / AB320.
  2. "DNA sequencing and analysis of a 24.7 kb segment encompassing centromere CEN11 of Saccharomyces cerevisiae reveals nine previously unknown open reading frames."
    Duesterhoeft A., Philippsen P.
    Yeast 8:749-759(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKAPS_YEAST
AccessioniPrimary (citable) accession number: Q02196
Secondary accession number(s): D6VXT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 11, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.