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Protein

Anthranilate phosphoribosyltransferase, chloroplastic

Gene

PAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase beta subunit 2, chloroplastic (ASB2), Anthranilate synthase beta subunit 1, chloroplastic (ASB1), Anthranilate synthase alpha subunit 1, chloroplastic (ASA1), Anthranilate synthase alpha subunit 2, chloroplastic (ASA2)
  2. Anthranilate phosphoribosyltransferase, chloroplastic (PAT1)
  3. N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic (PAI2), N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (PAI3), N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (PAI1)
  4. Indole-3-glycerol phosphate synthase, chloroplastic (IGPS)
  5. Tryptophan synthase alpha chain (TRPA1), Tryptophan synthase alpha chain, chloroplastic (TSA1), Tryptophan synthase (AXX17_At5g53930), Tryptophan synthase (At5g54810), Tryptophan synthase (At5g28237), Tryptophan synthase (AXX17_At5g28490), Tryptophan synthase (AXX17_At4g31220), Tryptophan synthase (At5g28237), Tryptophan synthase beta chain 1, chloroplastic (TSB1), Tryptophan synthase (At4g27070), Tryptophan synthase beta chain 2, chloroplastic (TSB2), Tryptophan synthase (AXX17_At4g31220)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • anthranilate phosphoribosyltransferase activity Source: TAIR

GO - Biological processi

  • cellular amino acid biosynthetic process Source: UniProtKB-KW
  • tryptophan biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciARA:AT5G17990-MONOMER.
UniPathwayiUPA00035; UER00041.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate phosphoribosyltransferase, chloroplastic (EC:2.4.2.18)
Gene namesi
Name:PAT1
Ordered Locus Names:At5g17990
ORF Names:MCM23.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G17990.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000035788? – 444Anthranilate phosphoribosyltransferase, chloroplastic
Transit peptidei1 – ?ChloroplastSequence analysis

Proteomic databases

PaxDbiQ02166.
PRIDEiQ02166.

Expressioni

Gene expression databases

GenevisibleiQ02166. AT.

Interactioni

Protein-protein interaction databases

BioGridi16942. 6 interactors.
IntActiQ02166. 1 interactor.
STRINGi3702.AT5G17990.1.

Structurei

3D structure databases

ProteinModelPortaliQ02166.
SMRiQ02166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 189Phosphoribosyl pyrophosphate binding siteSequence analysis9

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1438. Eukaryota.
COG0547. LUCA.
HOGENOMiHOG000230451.
KOiK00766.
OMAiNNIENTR.
OrthoDBiEOG09360D2K.
PhylomeDBiQ02166.

Family and domain databases

Gene3Di3.40.1030.10. 2 hits.
HAMAPiMF_00211. TrpD. 1 hit.
InterProiIPR005940. Anthranilate_Pribosyl_Tfrase.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
[Graphical view]
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
TIGRFAMsiTIGR01245. trpD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVIAVATTSS IVSGIKLSGI LTSFNAVDDA SSSCGRSNLT GVRIFPTLSR
60 70 80 90 100
RRFSSIGAVS PIRGDAQSSF SRSSFACSQN LGLSGGFSAA EALPPACANA
110 120 130 140 150
SPSSIKSFNQ LIETLIDRVD LSETEAESSL EFLLNEANEA LISAFLVLLR
160 170 180 190 200
AKGETYEEIV GLARAMMKHA RKVEGLVDAV DIVGTGGDGA NTVNISTGSS
210 220 230 240 250
ILAAACGAKV AKQGNRSSSS ACGSADVLEA LGVVLDLGPE GIKRCVEEGG
260 270 280 290 300
IGFMMSPMYH PAMKIVGPVR KKLKIKTVFN ILGPMLNPAR VSYAVVGVYH
310 320 330 340 350
KDLVVKMAKA LQRFGMKRAL VVHSCGLDEM SPLGGGLVYD VTPEKIEEFS
360 370 380 390 400
FDPLDFGIPR CTLEDLRGGG PDYNADVLRR VLSGESGAIA DSLILNAAAA
410 420 430 440
LLVSNRVQTL AEGVTVAREV QSSGKAIKTL DSWINISNLA QKSQ
Length:444
Mass (Da):46,520
Last modified:July 1, 1993 - v1
Checksum:i397DFD3141BED2C0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39 – 41Missing in AAB02913 (Ref. 2) Curated3
Sequence conflicti65D → H in AAB02913 (Ref. 2) Curated1
Sequence conflicti76 – 78ACS → VCP in AAB02913 (Ref. 2) Curated3
Sequence conflicti89A → T in AAB02913 (Ref. 2) Curated1
Sequence conflicti98A → E in AAB02913 (Ref. 2) Curated1
Sequence conflicti124T → S in AAB02913 (Ref. 2) Curated1
Sequence conflicti234V → A in AAB02913 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96073 Genomic DNA. Translation: AAA32835.1.
U58942 Genomic DNA. Translation: AAB02913.1.
AB015473 Genomic DNA. Translation: BAB08398.1.
CP002688 Genomic DNA. Translation: AED92493.1.
AY054506 mRNA. Translation: AAK96697.1.
AY093298 mRNA. Translation: AAM13297.1.
RefSeqiNP_197300.1. NM_121804.5.
UniGeneiAt.19968.
At.71230.
At.75028.

Genome annotation databases

EnsemblPlantsiAT5G17990.1; AT5G17990.1; AT5G17990.
GeneIDi831666.
GrameneiAT5G17990.1; AT5G17990.1; AT5G17990.
KEGGiath:AT5G17990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96073 Genomic DNA. Translation: AAA32835.1.
U58942 Genomic DNA. Translation: AAB02913.1.
AB015473 Genomic DNA. Translation: BAB08398.1.
CP002688 Genomic DNA. Translation: AED92493.1.
AY054506 mRNA. Translation: AAK96697.1.
AY093298 mRNA. Translation: AAM13297.1.
RefSeqiNP_197300.1. NM_121804.5.
UniGeneiAt.19968.
At.71230.
At.75028.

3D structure databases

ProteinModelPortaliQ02166.
SMRiQ02166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16942. 6 interactors.
IntActiQ02166. 1 interactor.
STRINGi3702.AT5G17990.1.

Proteomic databases

PaxDbiQ02166.
PRIDEiQ02166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G17990.1; AT5G17990.1; AT5G17990.
GeneIDi831666.
GrameneiAT5G17990.1; AT5G17990.1; AT5G17990.
KEGGiath:AT5G17990.

Organism-specific databases

TAIRiAT5G17990.

Phylogenomic databases

eggNOGiKOG1438. Eukaryota.
COG0547. LUCA.
HOGENOMiHOG000230451.
KOiK00766.
OMAiNNIENTR.
OrthoDBiEOG09360D2K.
PhylomeDBiQ02166.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00041.
BioCyciARA:AT5G17990-MONOMER.

Miscellaneous databases

PROiQ02166.

Gene expression databases

GenevisibleiQ02166. AT.

Family and domain databases

Gene3Di3.40.1030.10. 2 hits.
HAMAPiMF_00211. TrpD. 1 hit.
InterProiIPR005940. Anthranilate_Pribosyl_Tfrase.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
[Graphical view]
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
TIGRFAMsiTIGR01245. trpD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRPD_ARATH
AccessioniPrimary (citable) accession number: Q02166
Secondary accession number(s): Q42557
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.