Ubiquitin-conjugating enzyme E2-18 kDa - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot Q02159 (UBC7_YEAST)

Last modified January 19, 2010. Version 96. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2-18 kDa
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase
    Ubiquitin carrier protein

Gene names

Name:	UBC7
Synonyms:	QRI8
Ordered Locus Names:	YMR022W
ORF Names:	YM9711.12

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Involved in resistance to cadmium poisoning.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Induction	By cadmium.
Miscellaneous	Present with 2100 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

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Ontologies

Keywords
Biological process	Cadmium resistance Ubl conjugation pathway
Ligand	ATP-binding Cadmium Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	ER-associated protein catabolic process Inferred from direct assay. Source: SGD chromatin assembly or disassembly Inferred from mutant phenotype. Source: SGD fungal-type cell wall organization Inferred from genetic interaction. Source: SGD post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Traceable author statement. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cadmium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCY1	P07278	1	EBI-19749,EBI-9475
SSM4	P40318	1	EBI-19749,EBI-18208

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 165

165

Ubiquitin-conjugating enzyme E2-18 kDa

PRO_0000082554

Sites

Active site

Glycyl thioester intermediate

Secondary structure

165

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q02159-1 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: D3D297847DBB462D
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FASTA

165

18,520

        10         20         30         40         50         60 
MSKTAQKRLL KELQQLIKDS PPGIVAGPKS ENNIFIWDCL IQGPPDTPYA DGVFNAKLEF 

        70         80         90        100        110        120 
PKDYPLSPPK LTFTPSILHP NIYPNGEVCI SILHSPGDDP NMYELAEERW SPVQSVEKIL 

       130        140        150        160 
LSVMSMLSEP NIESGANIDA CILWRDNRPE FERQVKLSIL KSLGF

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"QRI8, a novel ubiquitin-conjugating enzyme in Saccharomyces cerevisiae." Vassal A., Boulet A., Decoster E., Faye G. Biochim. Biophys. Acta 1132:211-213(1992) [PubMed: 1327148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204510 / AB320.
[2]	"Resistance to cadmium mediated by ubiquitin-dependent proteolysis." Jungmann J., Reins H.-A., Schobert C., Jentsch S. Nature 361:369-371(1993) [PubMed: 8381213] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Crystal structure of a class I ubiquitin conjugating enzyme (Ubc7) from Saccharomyces cerevisiae at 2.9-A resolution." Cook W.J., Martin P.D., Edwards B.F.P., Yamazaki R.K., Chau V. Biochemistry 36:1621-1627(1997) [PubMed: 9048545] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.93 ANGSTROMS).
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X66829 Genomic DNA. Translation: CAA47302.1.
X69100 Genomic DNA. Translation: CAA48846.1.
Z49211 Genomic DNA. Translation: CAA89125.1.
AY558116 Genomic DNA. Translation: AAS56442.1.

PIR

S28951.

RefSeq

NP_013735.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2UCZ	X-ray	2.93	A	1-165	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6583N.

IntAct

Q02159. 612 interactions.

STRING

Q02159.

Proteomic databases

PeptideAtlas

Q02159.

PRIDE

Q02159.

Genome annotation databases

Ensembl

YMR022W; YMR022W; YMR022W; Saccharomyces cerevisiae. [Genome view]

GeneID

855036.

KEGG

sce:YMR022W.

NMPDR

fig|4932.3.peg.4781.

Organism-specific databases

CYGD

YMR022w.

SGD

S000004624. QRI8.

Phylogenomic databases

eggNOG

HOGENOM

OMA

OrthoDB

PhylomeDB

Q02159.

Enzyme and pathway databases

BRENDA

6.3.2.19. 250.

Gene expression databases

ArrayExpress

Q02159.

Genevestigator

Q02159.

GermOnline

YMR022W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

SMART

SM00212. UBCc. 1 hit.
[Graphical view]

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

978248.

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Entry information

Entry name

UBC7_YEAST

Accession

Primary (citable) accession number: Q02159

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	January 19, 2010
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents