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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase

Gene

plc

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei337 – 3371PROSITE-ProRule annotation
Active sitei382 – 3821PROSITE-ProRule annotation
Binding sitei463 – 4631SubstrateBy similarity
Binding sitei566 – 5661SubstrateBy similarity
Binding sitei593 – 5931SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi490 – 50112PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: dictyBase
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. establishment of protein localization Source: dictyBase
  2. inositol trisphosphate biosynthetic process Source: dictyBase
  3. intracellular signal transduction Source: InterPro
  4. lipid catabolic process Source: UniProtKB-KW
  5. negative regulation of chemotaxis Source: dictyBase
  6. sorocarp development Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C
Short name:
PLC
Gene namesi
Name:plc
Synonyms:pipA
ORF Names:DDB_G0292736
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292736. plc.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8018011-phosphatidylinositol 4,5-bisphosphate phosphodiesterasePRO_0000088509Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei524 – 5241PhosphoserineSequence Analysis
Modified residuei531 – 5311Phosphothreonine; by PKA and PKGSequence Analysis

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Expressed at all stages; increase in expression in the culminating fruiting body and during starvation.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0201656.

Structurei

3D structure databases

ProteinModelPortaliQ02158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini206 – 24136EF-handPROSITE-ProRule annotationAdd
BLAST
Domaini322 – 464143PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini542 – 652111PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini661 – 765105C2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
InParanoidiQ02158.
KOiK05857.
OMAiEPMWIND.
PhylomeDBiQ02158.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTLTNSQDY SNVDLSLESL AEELYNIQSF NKDVILDSFD IDDYDHTQLD
60 70 80 90 100
TTIDFNKFKI GLTILKISSK GKPQKKKLIF DLARNQIVCG KKKKVNFSEI
110 120 130 140 150
DEIRVGHKTN IFNQFKSSKN LKEDIESIQQ SFSILFSGNL RKTMDFVCSD
160 170 180 190 200
IPERRQIVSA LYHVVQESKS VNNEYNFVKR EWDRVGKDSI DFSTLKKILA
210 220 230 240 250
RLNFTTSDAV LHNLMKFSDS NSDYHLDFSE FSNLLKLLRS HPEMKPVFYK
260 270 280 290 300
YNGGNGEWVP IQGMIDFFRI EQSEVWTVEQ CRDLIKKYHH ERLDCISFEN
310 320 330 340 350
FEEFICGEAN LAQYPHTSTV YQDTSKPLSY YFINSSHNTY LSGHQLKGLS
360 370 380 390 400
TSEMYTNTLR QGCKCVELDV WDGNDGDPII FHGNTLTSQI KFSHVCETIK
410 420 430 440 450
ARGFETSPYP VILSLEVHCS VPQQIMMANH MKEIFGEMLP TPLPEGTKEL
460 470 480 490 500
PTLDSLKYKI LLKGHTSHTH VSAVGNSSAS SSQSNIQTDD NDDDGAVDLT
510 520 530 540 550
EYDEVDDRSA SSSSSSFSLS FGSSGKKKKI TKIKIAPEFE ELIYLVSHGF
560 570 580 590 600
KSGNTTKEIP SYKIHSLVEE KVKQLVQSEP REVVEASQNH LLRVYPRGTR
610 620 630 640 650
FDSSNFDPMP GWSIGCQLAA LNQQTSSEPM WINDGMFSDN GGCGYVLKPP
660 670 680 690 700
CLLPGECETY DPTSPERIKS SKYSRLIVNV ISARQLPKYT KSTKGEVIDP
710 720 730 740 750
YVTLSIVGTH FDQKVEKTKV IDNNGFNPHW GEEFEFPLYN SQLSMLLIRV
760 770 780 790 800
DDKDKVGHNR IGHHCIRVEN IRPGYRILKL KNNFNRTIPL ANLLCKFTFV

E
Length:801
Mass (Da):91,280
Last modified:October 1, 1993 - v1
Checksum:iDB4FA8C829812DD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95783 mRNA. Translation: AAA33235.1.
AY392433 Genomic DNA. Translation: AAQ98875.1.
AAFI02000196 Genomic DNA. Translation: EAL61042.1.
PIRiA44165.
RefSeqiXP_629476.1. XM_629474.1.

Genome annotation databases

EnsemblProtistsiDDB0201656; DDB0201656; DDB_G0292736.
GeneIDi8628866.
KEGGiddi:DDB_G0292736.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95783 mRNA. Translation: AAA33235.1.
AY392433 Genomic DNA. Translation: AAQ98875.1.
AAFI02000196 Genomic DNA. Translation: EAL61042.1.
PIRiA44165.
RefSeqiXP_629476.1. XM_629474.1.

3D structure databases

ProteinModelPortaliQ02158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0201656.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0201656; DDB0201656; DDB_G0292736.
GeneIDi8628866.
KEGGiddi:DDB_G0292736.

Organism-specific databases

dictyBaseiDDB_G0292736. plc.

Phylogenomic databases

eggNOGiNOG149692.
InParanoidiQ02158.
KOiK05857.
OMAiEPMWIND.
PhylomeDBiQ02158.

Miscellaneous databases

PROiQ02158.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of a phosphoinositide-specific phospholipase C of Dictyostelium discoideum."
    Drayer A.L., van Haastert P.J.
    J. Biol. Chem. 267:18387-18392(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NC-4.
  2. "Identification of genes dependent on the MADS box transcription factor SrfA in Dictyostelium discoideum development."
    Escalante R., Iranfar N., Sastre L., Loomis W.F.
    Eukaryot. Cell 3:564-566(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPLC_DICDI
AccessioniPrimary (citable) accession number: Q02158
Secondary accession number(s): Q54CR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 29, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

It is uncertain whether Met-1 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.