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Q02158 (PLC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C
Short name=PLC
Gene names
Name:plc
Synonyms:pipA
ORF Names:DDB_G0292736
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length801 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Developmental stage

Expressed at all stages; increase in expression in the culminating fruiting body and during starvation.

Sequence similarities

Contains 1 C2 domain.

Contains 1 EF-hand domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Caution

It is uncertain whether Met-1 is the initiator.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8018011-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
PRO_0000088509

Regions

Domain206 – 24136EF-hand
Domain322 – 464143PI-PLC X-box
Domain542 – 652111PI-PLC Y-box
Domain661 – 765105C2
Calcium binding490 – 50112 Potential

Sites

Active site3371 By similarity
Active site3821 By similarity
Binding site4631Substrate By similarity
Binding site5661Substrate By similarity
Binding site5931Substrate By similarity

Amino acid modifications

Modified residue5241Phosphoserine Potential
Modified residue5311Phosphothreonine; by PKA and PKG Potential

Sequences

Sequence LengthMass (Da)Tools
Q02158 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: DB4FA8C829812DD9

FASTA80191,280
        10         20         30         40         50         60 
MDTLTNSQDY SNVDLSLESL AEELYNIQSF NKDVILDSFD IDDYDHTQLD TTIDFNKFKI 

        70         80         90        100        110        120 
GLTILKISSK GKPQKKKLIF DLARNQIVCG KKKKVNFSEI DEIRVGHKTN IFNQFKSSKN 

       130        140        150        160        170        180 
LKEDIESIQQ SFSILFSGNL RKTMDFVCSD IPERRQIVSA LYHVVQESKS VNNEYNFVKR 

       190        200        210        220        230        240 
EWDRVGKDSI DFSTLKKILA RLNFTTSDAV LHNLMKFSDS NSDYHLDFSE FSNLLKLLRS 

       250        260        270        280        290        300 
HPEMKPVFYK YNGGNGEWVP IQGMIDFFRI EQSEVWTVEQ CRDLIKKYHH ERLDCISFEN 

       310        320        330        340        350        360 
FEEFICGEAN LAQYPHTSTV YQDTSKPLSY YFINSSHNTY LSGHQLKGLS TSEMYTNTLR 

       370        380        390        400        410        420 
QGCKCVELDV WDGNDGDPII FHGNTLTSQI KFSHVCETIK ARGFETSPYP VILSLEVHCS 

       430        440        450        460        470        480 
VPQQIMMANH MKEIFGEMLP TPLPEGTKEL PTLDSLKYKI LLKGHTSHTH VSAVGNSSAS 

       490        500        510        520        530        540 
SSQSNIQTDD NDDDGAVDLT EYDEVDDRSA SSSSSSFSLS FGSSGKKKKI TKIKIAPEFE 

       550        560        570        580        590        600 
ELIYLVSHGF KSGNTTKEIP SYKIHSLVEE KVKQLVQSEP REVVEASQNH LLRVYPRGTR 

       610        620        630        640        650        660 
FDSSNFDPMP GWSIGCQLAA LNQQTSSEPM WINDGMFSDN GGCGYVLKPP CLLPGECETY 

       670        680        690        700        710        720 
DPTSPERIKS SKYSRLIVNV ISARQLPKYT KSTKGEVIDP YVTLSIVGTH FDQKVEKTKV 

       730        740        750        760        770        780 
IDNNGFNPHW GEEFEFPLYN SQLSMLLIRV DDKDKVGHNR IGHHCIRVEN IRPGYRILKL 

       790        800 
KNNFNRTIPL ANLLCKFTFV E 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of a phosphoinositide-specific phospholipase C of Dictyostelium discoideum."
Drayer A.L., van Haastert P.J.
J. Biol. Chem. 267:18387-18392(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: NC-4.
[2]"Identification of genes dependent on the MADS box transcription factor SrfA in Dictyostelium discoideum development."
Escalante R., Iranfar N., Sastre L., Loomis W.F.
Eukaryot. Cell 3:564-566(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95783 mRNA. Translation: AAA33235.1.
AY392433 Genomic DNA. Translation: AAQ98875.1.
AAFI02000196 Genomic DNA. Translation: EAL61042.1.
PIRA44165.
RefSeqXP_629476.1. XM_629474.1.

3D structure databases

ProteinModelPortalQ02158.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0201656.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0201656; DDB0201656; DDB_G0292736.
GeneID8628866.
KEGGddi:DDB_G0292736.

Organism-specific databases

dictyBaseDDB_G0292736. plc.

Phylogenomic databases

eggNOGNOG149692.
OMAEPMWIND.
PhylomeDBQ02158.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLC_DICDI
AccessionPrimary (citable) accession number: Q02158
Secondary accession number(s): Q54CR5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase