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Q02158

- PLC_DICDI

UniProt

Q02158 - PLC_DICDI

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase

Gene
plc, pipA, DDB_G0292736
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei337 – 3371 By similarity
Active sitei382 – 3821 By similarity
Binding sitei463 – 4631Substrate By similarity
Binding sitei566 – 5661Substrate By similarity
Binding sitei593 – 5931Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi490 – 50112 Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: dictyBase
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. establishment of protein localization Source: dictyBase
  2. inositol trisphosphate biosynthetic process Source: dictyBase
  3. intracellular signal transduction Source: InterPro
  4. lipid catabolic process Source: UniProtKB-KW
  5. negative regulation of chemotaxis Source: dictyBase
  6. sorocarp development Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_208797. Acetylcholine regulates insulin secretion.
REACT_222661. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
REACT_222710. Synthesis of IP3 and IP4 in the cytosol.
REACT_227026. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C
Short name:
PLC
Gene namesi
Name:plc
Synonyms:pipA
ORF Names:DDB_G0292736
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292736. plc.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8018011-phosphatidylinositol 4,5-bisphosphate phosphodiesterasePRO_0000088509Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei524 – 5241Phosphoserine Reviewed prediction
Modified residuei531 – 5311Phosphothreonine; by PKA and PKG Reviewed prediction

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Expressed at all stages; increase in expression in the culminating fruiting body and during starvation.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0201656.

Structurei

3D structure databases

ProteinModelPortaliQ02158.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini206 – 24136EF-handAdd
BLAST
Domaini322 – 464143PI-PLC X-boxAdd
BLAST
Domaini542 – 652111PI-PLC Y-boxAdd
BLAST
Domaini661 – 765105C2Add
BLAST

Sequence similaritiesi

Contains 1 C2 domain.
Contains 1 EF-hand domain.

Phylogenomic databases

eggNOGiNOG149692.
KOiK05857.
OMAiEPMWIND.
PhylomeDBiQ02158.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02158-1 [UniParc]FASTAAdd to Basket

« Hide

MDTLTNSQDY SNVDLSLESL AEELYNIQSF NKDVILDSFD IDDYDHTQLD    50
TTIDFNKFKI GLTILKISSK GKPQKKKLIF DLARNQIVCG KKKKVNFSEI 100
DEIRVGHKTN IFNQFKSSKN LKEDIESIQQ SFSILFSGNL RKTMDFVCSD 150
IPERRQIVSA LYHVVQESKS VNNEYNFVKR EWDRVGKDSI DFSTLKKILA 200
RLNFTTSDAV LHNLMKFSDS NSDYHLDFSE FSNLLKLLRS HPEMKPVFYK 250
YNGGNGEWVP IQGMIDFFRI EQSEVWTVEQ CRDLIKKYHH ERLDCISFEN 300
FEEFICGEAN LAQYPHTSTV YQDTSKPLSY YFINSSHNTY LSGHQLKGLS 350
TSEMYTNTLR QGCKCVELDV WDGNDGDPII FHGNTLTSQI KFSHVCETIK 400
ARGFETSPYP VILSLEVHCS VPQQIMMANH MKEIFGEMLP TPLPEGTKEL 450
PTLDSLKYKI LLKGHTSHTH VSAVGNSSAS SSQSNIQTDD NDDDGAVDLT 500
EYDEVDDRSA SSSSSSFSLS FGSSGKKKKI TKIKIAPEFE ELIYLVSHGF 550
KSGNTTKEIP SYKIHSLVEE KVKQLVQSEP REVVEASQNH LLRVYPRGTR 600
FDSSNFDPMP GWSIGCQLAA LNQQTSSEPM WINDGMFSDN GGCGYVLKPP 650
CLLPGECETY DPTSPERIKS SKYSRLIVNV ISARQLPKYT KSTKGEVIDP 700
YVTLSIVGTH FDQKVEKTKV IDNNGFNPHW GEEFEFPLYN SQLSMLLIRV 750
DDKDKVGHNR IGHHCIRVEN IRPGYRILKL KNNFNRTIPL ANLLCKFTFV 800
E 801
Length:801
Mass (Da):91,280
Last modified:October 1, 1993 - v1
Checksum:iDB4FA8C829812DD9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M95783 mRNA. Translation: AAA33235.1.
AY392433 Genomic DNA. Translation: AAQ98875.1.
AAFI02000196 Genomic DNA. Translation: EAL61042.1.
PIRiA44165.
RefSeqiXP_629476.1. XM_629474.1.

Genome annotation databases

EnsemblProtistsiDDB0201656; DDB0201656; DDB_G0292736.
GeneIDi8628866.
KEGGiddi:DDB_G0292736.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M95783 mRNA. Translation: AAA33235.1 .
AY392433 Genomic DNA. Translation: AAQ98875.1 .
AAFI02000196 Genomic DNA. Translation: EAL61042.1 .
PIRi A44165.
RefSeqi XP_629476.1. XM_629474.1.

3D structure databases

ProteinModelPortali Q02158.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0201656.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0201656 ; DDB0201656 ; DDB_G0292736 .
GeneIDi 8628866.
KEGGi ddi:DDB_G0292736.

Organism-specific databases

dictyBasei DDB_G0292736. plc.

Phylogenomic databases

eggNOGi NOG149692.
KOi K05857.
OMAi EPMWIND.
PhylomeDBi Q02158.

Enzyme and pathway databases

Reactomei REACT_208797. Acetylcholine regulates insulin secretion.
REACT_222661. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
REACT_222710. Synthesis of IP3 and IP4 in the cytosol.
REACT_227026. G alpha (q) signalling events.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of a phosphoinositide-specific phospholipase C of Dictyostelium discoideum."
    Drayer A.L., van Haastert P.J.
    J. Biol. Chem. 267:18387-18392(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NC-4.
  2. "Identification of genes dependent on the MADS box transcription factor SrfA in Dictyostelium discoideum development."
    Escalante R., Iranfar N., Sastre L., Loomis W.F.
    Eukaryot. Cell 3:564-566(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPLC_DICDI
AccessioniPrimary (citable) accession number: Q02158
Secondary accession number(s): Q54CR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 3, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

It is uncertain whether Met-1 is the initiator.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3