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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase

Gene

plc

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei337PROSITE-ProRule annotation1
Active sitei382PROSITE-ProRule annotation1
Binding sitei463SubstrateBy similarity1
Binding sitei566SubstrateBy similarity1
Binding sitei593SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi490 – 501PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • establishment of protein localization Source: dictyBase
  • inositol trisphosphate biosynthetic process Source: dictyBase
  • intracellular signal transduction Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
  • negative regulation of chemotaxis Source: dictyBase
  • regulation of phagocytosis Source: dictyBase
  • sorocarp development Source: dictyBase

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DDI-112043. PLC beta mediated events.
R-DDI-114604. GPVI-mediated activation cascade.
R-DDI-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-DDI-202433. Generation of second messenger molecules.
R-DDI-399997. Acetylcholine regulates insulin secretion.
R-DDI-416476. G alpha (q) signalling events.
R-DDI-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-DDI-5607764. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C
Short name:
PLC
Gene namesi
Name:plc
Synonyms:pipA
ORF Names:DDB_G0292736
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292736. plc.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
  • membrane Source: dictyBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000885091 – 8011-phosphatidylinositol 4,5-bisphosphate phosphodiesteraseAdd BLAST801

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei524PhosphoserineSequence analysis1
Modified residuei531Phosphothreonine; by PKA and PKGSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ02158.

Expressioni

Developmental stagei

Expressed at all stages; increase in expression in the culminating fruiting body and during starvation.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0201656.

Structurei

3D structure databases

ProteinModelPortaliQ02158.
SMRiQ02158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini206 – 241EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini322 – 464PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST143
Domaini542 – 652PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST111
Domaini661 – 765C2PROSITE-ProRule annotationAdd BLAST105

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
InParanoidiQ02158.
KOiK05857.
OMAiHELMTLD.
PhylomeDBiQ02158.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR011993. PH_dom-like.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiView protein in SMART
SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiView protein in PROSITE
PS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTLTNSQDY SNVDLSLESL AEELYNIQSF NKDVILDSFD IDDYDHTQLD
60 70 80 90 100
TTIDFNKFKI GLTILKISSK GKPQKKKLIF DLARNQIVCG KKKKVNFSEI
110 120 130 140 150
DEIRVGHKTN IFNQFKSSKN LKEDIESIQQ SFSILFSGNL RKTMDFVCSD
160 170 180 190 200
IPERRQIVSA LYHVVQESKS VNNEYNFVKR EWDRVGKDSI DFSTLKKILA
210 220 230 240 250
RLNFTTSDAV LHNLMKFSDS NSDYHLDFSE FSNLLKLLRS HPEMKPVFYK
260 270 280 290 300
YNGGNGEWVP IQGMIDFFRI EQSEVWTVEQ CRDLIKKYHH ERLDCISFEN
310 320 330 340 350
FEEFICGEAN LAQYPHTSTV YQDTSKPLSY YFINSSHNTY LSGHQLKGLS
360 370 380 390 400
TSEMYTNTLR QGCKCVELDV WDGNDGDPII FHGNTLTSQI KFSHVCETIK
410 420 430 440 450
ARGFETSPYP VILSLEVHCS VPQQIMMANH MKEIFGEMLP TPLPEGTKEL
460 470 480 490 500
PTLDSLKYKI LLKGHTSHTH VSAVGNSSAS SSQSNIQTDD NDDDGAVDLT
510 520 530 540 550
EYDEVDDRSA SSSSSSFSLS FGSSGKKKKI TKIKIAPEFE ELIYLVSHGF
560 570 580 590 600
KSGNTTKEIP SYKIHSLVEE KVKQLVQSEP REVVEASQNH LLRVYPRGTR
610 620 630 640 650
FDSSNFDPMP GWSIGCQLAA LNQQTSSEPM WINDGMFSDN GGCGYVLKPP
660 670 680 690 700
CLLPGECETY DPTSPERIKS SKYSRLIVNV ISARQLPKYT KSTKGEVIDP
710 720 730 740 750
YVTLSIVGTH FDQKVEKTKV IDNNGFNPHW GEEFEFPLYN SQLSMLLIRV
760 770 780 790 800
DDKDKVGHNR IGHHCIRVEN IRPGYRILKL KNNFNRTIPL ANLLCKFTFV

E
Length:801
Mass (Da):91,280
Last modified:October 1, 1993 - v1
Checksum:iDB4FA8C829812DD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95783 mRNA. Translation: AAA33235.1.
AY392433 Genomic DNA. Translation: AAQ98875.1.
AAFI02000196 Genomic DNA. Translation: EAL61042.1.
PIRiA44165.
RefSeqiXP_629476.1. XM_629474.1.

Genome annotation databases

EnsemblProtistsiEAL61042; EAL61042; DDB_G0292736.
GeneIDi8628866.
KEGGiddi:DDB_G0292736.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPLC_DICDI
AccessioniPrimary (citable) accession number: Q02158
Secondary accession number(s): Q54CR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 7, 2017
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

It is uncertain whether Met-1 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase