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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase

Gene

plc

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Caution

It is uncertain whether Met-1 is the initiator.Curated

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei337PROSITE-ProRule annotation1
Active sitei382PROSITE-ProRule annotation1
Binding sitei463SubstrateBy similarity1
Binding sitei566SubstrateBy similarity1
Binding sitei593SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi490 – 501PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • establishment of protein localization Source: dictyBase
  • inositol trisphosphate biosynthetic process Source: dictyBase
  • intracellular signal transduction Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
  • negative regulation of chemotaxis Source: dictyBase
  • regulation of phagocytosis Source: dictyBase
  • sorocarp development Source: dictyBase

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DDI-112043 PLC beta mediated events
R-DDI-114604 GPVI-mediated activation cascade
R-DDI-1855204 Synthesis of IP3 and IP4 in the cytosol
R-DDI-202433 Generation of second messenger molecules
R-DDI-399997 Acetylcholine regulates insulin secretion
R-DDI-416476 G alpha (q) signalling events
R-DDI-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-DDI-5607764 CLEC7A (Dectin-1) signaling

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C
Short name:
PLC
Gene namesi
Name:plc
Synonyms:pipA
ORF Names:DDB_G0292736
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292736 plc

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
  • membrane Source: dictyBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000885091 – 8011-phosphatidylinositol 4,5-bisphosphate phosphodiesteraseAdd BLAST801

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei524PhosphoserineSequence analysis1
Modified residuei531Phosphothreonine; by PKA and PKGSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ02158

Expressioni

Developmental stagei

Expressed at all stages; increase in expression in the culminating fruiting body and during starvation.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0201656

Structurei

3D structure databases

ProteinModelPortaliQ02158
SMRiQ02158
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini206 – 241EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini322 – 464PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST143
Domaini542 – 652PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST111
Domaini661 – 765C2PROSITE-ProRule annotationAdd BLAST105

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
InParanoidiQ02158
KOiK05857
OMAiMTHPLND
PhylomeDBiQ02158

Family and domain databases

Gene3Di2.30.29.30, 1 hit
2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR002048 EF_hand_dom
IPR011993 PH-like_dom_sf
IPR001192 PI-PLC_fam
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q02158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTLTNSQDY SNVDLSLESL AEELYNIQSF NKDVILDSFD IDDYDHTQLD
60 70 80 90 100
TTIDFNKFKI GLTILKISSK GKPQKKKLIF DLARNQIVCG KKKKVNFSEI
110 120 130 140 150
DEIRVGHKTN IFNQFKSSKN LKEDIESIQQ SFSILFSGNL RKTMDFVCSD
160 170 180 190 200
IPERRQIVSA LYHVVQESKS VNNEYNFVKR EWDRVGKDSI DFSTLKKILA
210 220 230 240 250
RLNFTTSDAV LHNLMKFSDS NSDYHLDFSE FSNLLKLLRS HPEMKPVFYK
260 270 280 290 300
YNGGNGEWVP IQGMIDFFRI EQSEVWTVEQ CRDLIKKYHH ERLDCISFEN
310 320 330 340 350
FEEFICGEAN LAQYPHTSTV YQDTSKPLSY YFINSSHNTY LSGHQLKGLS
360 370 380 390 400
TSEMYTNTLR QGCKCVELDV WDGNDGDPII FHGNTLTSQI KFSHVCETIK
410 420 430 440 450
ARGFETSPYP VILSLEVHCS VPQQIMMANH MKEIFGEMLP TPLPEGTKEL
460 470 480 490 500
PTLDSLKYKI LLKGHTSHTH VSAVGNSSAS SSQSNIQTDD NDDDGAVDLT
510 520 530 540 550
EYDEVDDRSA SSSSSSFSLS FGSSGKKKKI TKIKIAPEFE ELIYLVSHGF
560 570 580 590 600
KSGNTTKEIP SYKIHSLVEE KVKQLVQSEP REVVEASQNH LLRVYPRGTR
610 620 630 640 650
FDSSNFDPMP GWSIGCQLAA LNQQTSSEPM WINDGMFSDN GGCGYVLKPP
660 670 680 690 700
CLLPGECETY DPTSPERIKS SKYSRLIVNV ISARQLPKYT KSTKGEVIDP
710 720 730 740 750
YVTLSIVGTH FDQKVEKTKV IDNNGFNPHW GEEFEFPLYN SQLSMLLIRV
760 770 780 790 800
DDKDKVGHNR IGHHCIRVEN IRPGYRILKL KNNFNRTIPL ANLLCKFTFV

E
Length:801
Mass (Da):91,280
Last modified:October 1, 1993 - v1
Checksum:iDB4FA8C829812DD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95783 mRNA Translation: AAA33235.1
AY392433 Genomic DNA Translation: AAQ98875.1
AAFI02000196 Genomic DNA Translation: EAL61042.1
PIRiA44165
RefSeqiXP_629476.1, XM_629474.1

Genome annotation databases

EnsemblProtistsiEAL61042; EAL61042; DDB_G0292736
GeneIDi8628866
KEGGiddi:DDB_G0292736

Similar proteinsi

Entry informationi

Entry nameiPLC_DICDI
AccessioniPrimary (citable) accession number: Q02158
Secondary accession number(s): Q54CR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health