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Q02158

- PLC_DICDI

UniProt

Q02158 - PLC_DICDI

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase

Gene

plc

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei337 – 3371PROSITE-ProRule annotation
    Active sitei382 – 3821PROSITE-ProRule annotation
    Binding sitei463 – 4631SubstrateBy similarity
    Binding sitei566 – 5661SubstrateBy similarity
    Binding sitei593 – 5931SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi490 – 50112PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: dictyBase
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. establishment of protein localization Source: dictyBase
    2. inositol trisphosphate biosynthetic process Source: dictyBase
    3. intracellular signal transduction Source: InterPro
    4. lipid catabolic process Source: UniProtKB-KW
    5. negative regulation of chemotaxis Source: dictyBase
    6. sorocarp development Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_208797. Acetylcholine regulates insulin secretion.
    REACT_222661. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
    REACT_222710. Synthesis of IP3 and IP4 in the cytosol.
    REACT_227026. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C
    Short name:
    PLC
    Gene namesi
    Name:plc
    Synonyms:pipA
    ORF Names:DDB_G0292736
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0292736. plc.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8018011-phosphatidylinositol 4,5-bisphosphate phosphodiesterasePRO_0000088509Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei524 – 5241PhosphoserineSequence Analysis
    Modified residuei531 – 5311Phosphothreonine; by PKA and PKGSequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Developmental stagei

    Expressed at all stages; increase in expression in the culminating fruiting body and during starvation.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0201656.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02158.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini206 – 24136EF-handPROSITE-ProRule annotationAdd
    BLAST
    Domaini322 – 464143PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini542 – 652111PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini661 – 765105C2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    KOiK05857.
    OMAiEPMWIND.
    PhylomeDBiQ02158.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q02158-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDTLTNSQDY SNVDLSLESL AEELYNIQSF NKDVILDSFD IDDYDHTQLD    50
    TTIDFNKFKI GLTILKISSK GKPQKKKLIF DLARNQIVCG KKKKVNFSEI 100
    DEIRVGHKTN IFNQFKSSKN LKEDIESIQQ SFSILFSGNL RKTMDFVCSD 150
    IPERRQIVSA LYHVVQESKS VNNEYNFVKR EWDRVGKDSI DFSTLKKILA 200
    RLNFTTSDAV LHNLMKFSDS NSDYHLDFSE FSNLLKLLRS HPEMKPVFYK 250
    YNGGNGEWVP IQGMIDFFRI EQSEVWTVEQ CRDLIKKYHH ERLDCISFEN 300
    FEEFICGEAN LAQYPHTSTV YQDTSKPLSY YFINSSHNTY LSGHQLKGLS 350
    TSEMYTNTLR QGCKCVELDV WDGNDGDPII FHGNTLTSQI KFSHVCETIK 400
    ARGFETSPYP VILSLEVHCS VPQQIMMANH MKEIFGEMLP TPLPEGTKEL 450
    PTLDSLKYKI LLKGHTSHTH VSAVGNSSAS SSQSNIQTDD NDDDGAVDLT 500
    EYDEVDDRSA SSSSSSFSLS FGSSGKKKKI TKIKIAPEFE ELIYLVSHGF 550
    KSGNTTKEIP SYKIHSLVEE KVKQLVQSEP REVVEASQNH LLRVYPRGTR 600
    FDSSNFDPMP GWSIGCQLAA LNQQTSSEPM WINDGMFSDN GGCGYVLKPP 650
    CLLPGECETY DPTSPERIKS SKYSRLIVNV ISARQLPKYT KSTKGEVIDP 700
    YVTLSIVGTH FDQKVEKTKV IDNNGFNPHW GEEFEFPLYN SQLSMLLIRV 750
    DDKDKVGHNR IGHHCIRVEN IRPGYRILKL KNNFNRTIPL ANLLCKFTFV 800
    E 801
    Length:801
    Mass (Da):91,280
    Last modified:October 1, 1993 - v1
    Checksum:iDB4FA8C829812DD9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95783 mRNA. Translation: AAA33235.1.
    AY392433 Genomic DNA. Translation: AAQ98875.1.
    AAFI02000196 Genomic DNA. Translation: EAL61042.1.
    PIRiA44165.
    RefSeqiXP_629476.1. XM_629474.1.

    Genome annotation databases

    EnsemblProtistsiDDB0201656; DDB0201656; DDB_G0292736.
    GeneIDi8628866.
    KEGGiddi:DDB_G0292736.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95783 mRNA. Translation: AAA33235.1 .
    AY392433 Genomic DNA. Translation: AAQ98875.1 .
    AAFI02000196 Genomic DNA. Translation: EAL61042.1 .
    PIRi A44165.
    RefSeqi XP_629476.1. XM_629474.1.

    3D structure databases

    ProteinModelPortali Q02158.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0201656.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0201656 ; DDB0201656 ; DDB_G0292736 .
    GeneIDi 8628866.
    KEGGi ddi:DDB_G0292736.

    Organism-specific databases

    dictyBasei DDB_G0292736. plc.

    Phylogenomic databases

    eggNOGi NOG149692.
    KOi K05857.
    OMAi EPMWIND.
    PhylomeDBi Q02158.

    Enzyme and pathway databases

    Reactomei REACT_208797. Acetylcholine regulates insulin secretion.
    REACT_222661. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
    REACT_222710. Synthesis of IP3 and IP4 in the cytosol.
    REACT_227026. G alpha (q) signalling events.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of a phosphoinositide-specific phospholipase C of Dictyostelium discoideum."
      Drayer A.L., van Haastert P.J.
      J. Biol. Chem. 267:18387-18392(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: NC-4.
    2. "Identification of genes dependent on the MADS box transcription factor SrfA in Dictyostelium discoideum development."
      Escalante R., Iranfar N., Sastre L., Loomis W.F.
      Eukaryot. Cell 3:564-566(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiPLC_DICDI
    AccessioniPrimary (citable) accession number: Q02158
    Secondary accession number(s): Q54CR5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3