Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamine synthetase 2

Gene

glnII

Organism
Rhizobium leguminosarum bv. phaseoli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulationi

Transferase activity is inhibited by NH4Cl.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase 2 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase II
Glutamine synthetase II
Short name:
GSII
Gene namesi
Name:glnII
OrganismiRhizobium leguminosarum bv. phaseoli
Taxonomic identifieri385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 326325Glutamine synthetase 2PRO_0000153224Add
BLAST

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliQ02154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKFKLEYIW LDGYTPVPNL RGKTQIKEFD EFPTLEQLPL WGFDGSSTMQ
60 70 80 90 100
AEGSSDCVLK PVAIYPDPAR TNGALVMCEV MMPDGHAHAS NARATILDDE
110 120 130 140 150
DAWFGFEQEY FFYQNGRPLG FPEQGYPAPQ PYYTGVGYSN VGDVAREIVE
160 170 180 190 200
EHLDLCLAAG INHEGINAEV AKGQWEFQIF GKGSKKAADQ IWMARYLLQR
210 220 230 240 250
LTEKYGIDIE YHCKPLGDTD WNGSGMHCNF STKYLREVGG KEYFEALMAS
260 270 280 290 300
SDKNLMDHIA VYGPDNDKRL TGKHETAPWN KFSYGVADRG ASIRVPHSFI
310 320
KNDYKGYLED RRPNSQGDPY QIVRRF
Length:326
Mass (Da):36,979
Last modified:January 23, 2007 - v3
Checksum:i637AE03B83D1F840
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67296 Genomic DNA. Translation: CAA47710.1.
PIRiS26216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67296 Genomic DNA. Translation: CAA47710.1.
PIRiS26216.

3D structure databases

ProteinModelPortaliQ02154.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Activation of the Rhizobium leguminosarum glnII gene by NtrC is dependent on upstream DNA sequences."
    Patriarca E.J., Chiurazzi M., Manco G., Riccio A., Lamberti A., Paolis A., Rossi M., Defez R., Iaccarino M.
    Mol. Gen. Genet. 234:337-345(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LPR1105.
  2. "Dissociation by NH4Cl treatment of the enzymic activities of glutamine synthetase II from Rhizobium leguminosarum biovar viceae."
    Manco G., Rossi M., Defez R., Lamberti A., Percuoco G., Iaccarino M.
    J. Gen. Microbiol. 138:1453-1460(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-27.
    Strain: LPR1105.

Entry informationi

Entry nameiGLNA2_RHILP
AccessioniPrimary (citable) accession number: Q02154
Secondary accession number(s): Q9R5J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 72 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.