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Q02154

- GLNA2_RHILP

UniProt

Q02154 - GLNA2_RHILP

Protein

Glutamine synthetase 2

Gene

glnII

Organism
Rhizobium leguminosarum bv. phaseoli
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Enzyme regulationi

    Transferase activity is inhibited by NH4Cl.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase 2 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase II
    Glutamine synthetase II
    Short name:
    GSII
    Gene namesi
    Name:glnII
    OrganismiRhizobium leguminosarum bv. phaseoli
    Taxonomic identifieri385 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 326325Glutamine synthetase 2PRO_0000153224Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ02154.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q02154-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKFKLEYIW LDGYTPVPNL RGKTQIKEFD EFPTLEQLPL WGFDGSSTMQ    50
    AEGSSDCVLK PVAIYPDPAR TNGALVMCEV MMPDGHAHAS NARATILDDE 100
    DAWFGFEQEY FFYQNGRPLG FPEQGYPAPQ PYYTGVGYSN VGDVAREIVE 150
    EHLDLCLAAG INHEGINAEV AKGQWEFQIF GKGSKKAADQ IWMARYLLQR 200
    LTEKYGIDIE YHCKPLGDTD WNGSGMHCNF STKYLREVGG KEYFEALMAS 250
    SDKNLMDHIA VYGPDNDKRL TGKHETAPWN KFSYGVADRG ASIRVPHSFI 300
    KNDYKGYLED RRPNSQGDPY QIVRRF 326
    Length:326
    Mass (Da):36,979
    Last modified:January 23, 2007 - v3
    Checksum:i637AE03B83D1F840
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67296 Genomic DNA. Translation: CAA47710.1.
    PIRiS26216.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67296 Genomic DNA. Translation: CAA47710.1 .
    PIRi S26216.

    3D structure databases

    ProteinModelPortali Q02154.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Activation of the Rhizobium leguminosarum glnII gene by NtrC is dependent on upstream DNA sequences."
      Patriarca E.J., Chiurazzi M., Manco G., Riccio A., Lamberti A., Paolis A., Rossi M., Defez R., Iaccarino M.
      Mol. Gen. Genet. 234:337-345(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LPR1105.
    2. "Dissociation by NH4Cl treatment of the enzymic activities of glutamine synthetase II from Rhizobium leguminosarum biovar viceae."
      Manco G., Rossi M., Defez R., Lamberti A., Percuoco G., Iaccarino M.
      J. Gen. Microbiol. 138:1453-1460(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-27.
      Strain: LPR1105.

    Entry informationi

    Entry nameiGLNA2_RHILP
    AccessioniPrimary (citable) accession number: Q02154
    Secondary accession number(s): Q9R5J0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 70 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3