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Protein

Glutamine synthetase

Gene

glnII

Organism
Rhizobium leguminosarum bv. phaseoli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.1 Publication

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Transferase activity is inhibited by NH4Cl.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107MagnesiumBy similarity1
Metal bindingi109MagnesiumBy similarity1
Binding sitei164ATPBy similarity1
Metal bindingi169MagnesiumBy similarity1
Metal bindingi176MagnesiumBy similarity1
Binding sitei275L-glutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase1 Publication (EC:6.3.1.2By similarity)
Short name:
GS1 Publication
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase II1 Publication
Short name:
GSII1 Publication
Gene namesi
Name:glnII1 Publication
OrganismiRhizobium leguminosarum bv. phaseoli
Taxonomic identifieri385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001532242 – 326Glutamine synthetaseAdd BLAST325

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein NtrC.1 Publication

Interactioni

Subunit structurei

Homooctamer and homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ02154.
SMRiQ02154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKFKLEYIW LDGYTPVPNL RGKTQIKEFD EFPTLEQLPL WGFDGSSTMQ
60 70 80 90 100
AEGSSDCVLK PVAIYPDPAR TNGALVMCEV MMPDGHAHAS NARATILDDE
110 120 130 140 150
DAWFGFEQEY FFYQNGRPLG FPEQGYPAPQ PYYTGVGYSN VGDVAREIVE
160 170 180 190 200
EHLDLCLAAG INHEGINAEV AKGQWEFQIF GKGSKKAADQ IWMARYLLQR
210 220 230 240 250
LTEKYGIDIE YHCKPLGDTD WNGSGMHCNF STKYLREVGG KEYFEALMAS
260 270 280 290 300
SDKNLMDHIA VYGPDNDKRL TGKHETAPWN KFSYGVADRG ASIRVPHSFI
310 320
KNDYKGYLED RRPNSQGDPY QIVRRF
Length:326
Mass (Da):36,979
Last modified:January 23, 2007 - v3
Checksum:i637AE03B83D1F840
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67296 Genomic DNA. Translation: CAA47710.1.
PIRiS26216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67296 Genomic DNA. Translation: CAA47710.1.
PIRiS26216.

3D structure databases

ProteinModelPortaliQ02154.
SMRiQ02154.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA2_RHILP
AccessioniPrimary (citable) accession number: Q02154
Secondary accession number(s): Q9R5J0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: April 12, 2017
This is version 78 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.