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Protein

Guanylate cyclase soluble subunit beta-1

Gene

GUCY1B1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.By similarity1 Publication

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.1 Publication

Cofactori

hemeBy similarityNote: Binds 1 or 2 heme groups per heterodimer. Heme is required for responding to nitric oxide, but not for catalytic activity.By similarity

Enzyme regulationi

Activated by nitric oxide in the presence of magnesium or manganese ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105Iron (heme proximal ligand)By similarity1

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: UniProtKB
  • heme binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • signaling receptor activity Source: ProtInc

GO - Biological processi

  • blood circulation Source: ProtInc
  • cellular response to nitric oxide Source: UniProtKB
  • cGMP biosynthetic process Source: UniProtKB
  • nitric oxide-cGMP-mediated signaling pathway Source: UniProtKB
  • nitric oxide mediated signal transduction Source: ProtInc

Keywordsi

Molecular functionLyase
Biological processcGMP biosynthesis
LigandGTP-binding, Heme, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-392154 Nitric oxide stimulates guanylate cyclase
SIGNORiQ02153

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase soluble subunit beta-1Curated (EC:4.6.1.23 Publications)
Short name:
GCS-beta-1
Alternative name(s):
Guanylate cyclase soluble subunit beta-31 Publication
Short name:
GCS-beta-3
Soluble guanylate cyclase small subunit
Gene namesi
Name:GUCY1B1Imported
Synonyms:GUC1B3, GUCSB3, GUCY1B3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000061918.12
HGNCiHGNC:4687 GUCY1B1
MIMi139397 gene
neXtProtiNX_Q02153

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi2983
OpenTargetsiENSG00000061918
PharmGKBiPA29068

Chemistry databases

ChEMBLiCHEMBL3137281
GuidetoPHARMACOLOGYi1290

Polymorphism and mutation databases

BioMutaiGUCY1B3
DMDMi399328

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741161 – 619Guanylate cyclase soluble subunit beta-1Add BLAST619

Proteomic databases

MaxQBiQ02153
PaxDbiQ02153
PeptideAtlasiQ02153
PRIDEiQ02153

PTM databases

iPTMnetiQ02153
PhosphoSitePlusiQ02153

Expressioni

Tissue specificityi

Detected in brain cortex and cerebellum (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000061918
CleanExiHS_GUCY1B3
ExpressionAtlasiQ02153 baseline and differential
GenevisibleiQ02153 HS

Organism-specific databases

HPAiCAB010890
HPA020870

Interactioni

Subunit structurei

The active enzyme is formed by a heterodimer of an alpha and a beta subunit. Heterodimer with GUCY1A1 (PubMed:1352257, PubMed:23505436, PubMed:24669844). Can also form inactive homodimers in vitro (PubMed:23505436, PubMed:24669844).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GUCY1A1Q021082EBI-6911707,EBI-3910037

Protein-protein interaction databases

BioGridi109238, 16 interactors
CORUMiQ02153
IntActiQ02153, 2 interactors
STRINGi9606.ENSP00000264424

Chemistry databases

BindingDBiQ02153

Structurei

Secondary structure

1619
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi415 – 427Combined sources13
Helixi430 – 436Combined sources7
Helixi443 – 461Combined sources19
Turni463 – 465Combined sources3
Beta strandi470 – 472Combined sources3
Beta strandi479 – 487Combined sources9
Helixi492 – 507Combined sources16
Beta strandi519 – 533Combined sources15
Beta strandi535 – 537Combined sources3
Beta strandi539 – 544Combined sources6
Helixi545 – 555Combined sources11
Beta strandi561 – 565Combined sources5
Helixi566 – 571Combined sources6
Turni575 – 577Combined sources3
Beta strandi582 – 590Combined sources9
Beta strandi599 – 607Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WZ1X-ray1.63A/B408-619[»]
3UVJX-ray2.08B/D408-619[»]
4NI2X-ray1.90B408-608[»]
ProteinModelPortaliQ02153
SMRiQ02153
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ02153

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini421 – 554Guanylate cyclasePROSITE-ProRule annotationAdd BLAST134

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4171 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000118959
HOGENOMiHOG000220903
HOVERGENiHBG051715
InParanoidiQ02153
KOiK12319
PhylomeDBiQ02153
TreeFamiTF351403

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
3.90.1520.10, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR038158 H-NOX_domain_sf
IPR011644 Heme_NO-bd
IPR011645 HNOB_dom_associated
IPR024096 NO_sig/Golgi_transp_ligand-bd
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00211 Guanylate_cyc, 1 hit
PF07700 HNOB, 1 hit
PF07701 HNOBA, 1 hit
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF111126 SSF111126, 1 hit
SSF55073 SSF55073, 1 hit
PROSITEiView protein in PROSITE
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform HSGC-1 (identifier: Q02153-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD
60 70 80 90 100
LVAAASKVLN LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN
110 120 130 140 150
LDALHDHLAT IYPGMRAPSF RCTDAEKGKG LILHYYSERE GLQDIVIGII
160 170 180 190 200
KTVAQQIHGT EIDMKVIQQR NEECDHTQFL IEEKESKEED FYEDLDRFEE
210 220 230 240 250
NGTQESRISP YTFCKAFPFH IIFDRDLVVT QCGNAIYRVL PQLQPGNCSL
260 270 280 290 300
LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKL ECEDELTGTE
310 320 330 340 350
ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR
360 370 380 390 400
DLVLLGEQFR EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTLLYSVLPP
410 420 430 440 450
SVANELRHKR PVPAKRYDNV TILFSGIVGF NAFCSKHASG EGAMKIVNLL
460 470 480 490 500
NDLYTRFDTL TDSRKNPFVY KVETVGDKYM TVSGLPEPCI HHARSICHLA
510 520 530 540 550
LDMMEIAGQV QVDGESVQIT IGIHTGEVVT GVIGQRMPRY CLFGNTVNLT
560 570 580 590 600
SRTETTGEKG KINVSEYTYR CLMSPENSDP QFHLEHRGPV SMKGKKEPMQ
610
VWFLSRKNTG TEETKQDDD
Length:619
Mass (Da):70,514
Last modified:July 1, 1993 - v1
Checksum:i231E4E660DE02AA1
GO
Isoform HSGC-2 (identifier: Q02153-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     393-425: Missing.

Show »
Length:586
Mass (Da):66,738
Checksum:i59818154086FD4A3
GO
Isoform 3 (identifier: Q02153-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: MYGFVNHALELLVIRNYGPEVWEDIK → MLMCFI

Note: No experimental confirmation available.
Show »
Length:599
Mass (Da):68,165
Checksum:i93F6129D51BECB23
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0543651 – 26MYGFV…WEDIK → MLMCFI in isoform 3. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_001813393 – 425Missing in isoform HSGC-2. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66533 mRNA Translation: CAA47144.1
AF020340 mRNA Translation: AAB94877.1
AK296680 mRNA Translation: BAH12412.1
AC114761 Genomic DNA No translation available.
BC047620 mRNA Translation: AAH47620.2
CCDSiCCDS47154.1 [Q02153-1]
CCDS77976.1 [Q02153-2]
CCDS77977.1 [Q02153-3]
PIRiS23097
RefSeqiNP_000848.1, NM_000857.3 [Q02153-1]
NP_001278881.1, NM_001291952.1 [Q02153-3]
NP_001278882.1, NM_001291953.1
NP_001278883.1, NM_001291954.1 [Q02153-2]
UniGeneiHs.77890

Genome annotation databases

EnsembliENST00000264424; ENSP00000264424; ENSG00000061918 [Q02153-1]
ENST00000503520; ENSP00000420842; ENSG00000061918 [Q02153-2]
ENST00000505764; ENSP00000426319; ENSG00000061918 [Q02153-3]
GeneIDi2983
KEGGihsa:2983
UCSCiuc003ipc.4 human [Q02153-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGCYB1_HUMAN
AccessioniPrimary (citable) accession number: Q02153
Secondary accession number(s): B7Z426, Q86WY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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