Guanylate cyclase soluble subunit beta-1

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Regions

Sites

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Preferred order of sections

Molecule processing

Regions

Sites

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

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Q02153 (GCYB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 128. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
Guanylate cyclase soluble subunit beta-1
Short name=GCS-beta-1
EC=4.6.1.2

Alternative name(s):
Guanylate cyclase soluble subunit beta-3
Short name=GCS-beta-3
Soluble guanylate cyclase small subunit

Gene names

Name:	GUCY1B3
Synonyms:	GUC1B3, GUCSB3, GUCY1B1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Catalytic activity	GTP = 3',5'-cyclic GMP + diphosphate.
Cofactor	Binds 1 or 2 heme groups per heterodimer By similarity.
Enzyme regulation	Activated by nitric oxide in the presence of magnesium or manganese ions.
Subunit structure	Heterodimer of an alpha and a beta chain.
Subcellular location	Cytoplasm.
Miscellaneous	There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 1 guanylate cyclase domain.

Keywords
Biological process	cGMP biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Heme Iron Metal-binding Nucleotide-binding
Molecular function	Lyase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	blood circulation Traceable author statement PubMed 9742212. Source: ProtInc blood coagulation Traceable author statement. Source: Reactome nitric oxide mediated signal transduction Traceable author statement PubMed 9742212. Source: ProtInc
Cellular_component	guanylate cyclase complex, soluble Traceable author statement Ref.1. Source: ProtInc intracellular membrane-bounded organelle Inferred from direct assay. Source: HPA
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate cyclase activity Traceable author statement PubMed 9742212. Source: ProtInc heme binding Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW receptor activity Traceable author statement PubMed 9742212. Source: ProtInc
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 619

619

Guanylate cyclase soluble subunit beta-1

PRO_0000074116

Domain

421 – 554

134

Guanylate cyclase

Metal binding

105

Iron (heme proximal ligand) By similarity

Alternative sequence

393 – 425

Missing in isoform HSGC-2.

VSP_001813

619

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform HSGC-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 231E4E660DE02AA1 Show »	FASTA	619	70,514
10 20 30 40 50 60 MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD LVAAASKVLN 70 80 90 100 110 120 LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN LDALHDHLAT IYPGMRAPSF 130 140 150 160 170 180 RCTDAEKGKG LILHYYSERE GLQDIVIGII KTVAQQIHGT EIDMKVIQQR NEECDHTQFL 190 200 210 220 230 240 IEEKESKEED FYEDLDRFEE NGTQESRISP YTFCKAFPFH IIFDRDLVVT QCGNAIYRVL 250 260 270 280 290 300 PQLQPGNCSL LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKL ECEDELTGTE 310 320 330 340 350 360 ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR DLVLLGEQFR 370 380 390 400 410 420 EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTLLYSVLPP SVANELRHKR PVPAKRYDNV 430 440 450 460 470 480 TILFSGIVGF NAFCSKHASG EGAMKIVNLL NDLYTRFDTL TDSRKNPFVY KVETVGDKYM 490 500 510 520 530 540 TVSGLPEPCI HHARSICHLA LDMMEIAGQV QVDGESVQIT IGIHTGEVVT GVIGQRMPRY 550 560 570 580 590 600 CLFGNTVNLT SRTETTGEKG KINVSEYTYR CLMSPENSDP QFHLEHRGPV SMKGKKEPMQ 610 VWFLSRKNTG TEETKQDDD « Hide
Isoform HSGC-2 [UniParc]. Checksum: 59818154086FD4A3 Show »	FASTA	586	66,738
10 20 30 40 50 60 MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD LVAAASKVLN 70 80 90 100 110 120 LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN LDALHDHLAT IYPGMRAPSF 130 140 150 160 170 180 RCTDAEKGKG LILHYYSERE GLQDIVIGII KTVAQQIHGT EIDMKVIQQR NEECDHTQFL 190 200 210 220 230 240 IEEKESKEED FYEDLDRFEE NGTQESRISP YTFCKAFPFH IIFDRDLVVT QCGNAIYRVL 250 260 270 280 290 300 PQLQPGNCSL LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKL ECEDELTGTE 310 320 330 340 350 360 ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR DLVLLGEQFR 370 380 390 400 410 420 EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTGIVGFNAF CSKHASGEGA MKIVNLLNDL 430 440 450 460 470 480 YTRFDTLTDS RKNPFVYKVE TVGDKYMTVS GLPEPCIHHA RSICHLALDM MEIAGQVQVD 490 500 510 520 530 540 GESVQITIGI HTGEVVTGVI GQRMPRYCLF GNTVNLTSRT ETTGEKGKIN VSEYTYRCLM 550 560 570 580 SPENSDPQFH LEHRGPVSMK GKKEPMQVWF LSRKNTGTEE TKQDDD « Hide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the cDNAs coding for the two subunits of soluble guanylyl cyclase from human brain." Giuili G., Scholl U., Bulle F., Guellaeen G. FEBS Lett. 304:83-88(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	Gansemans Y., Brouckaert P., Fiers W. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM HSGC-2). Tissue: Kidney.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM HSGC-1). Tissue: Brain.
[4]	"Heterogeneity in human soluble guanylate cyclase due to alternative splicing." Chhajilani V., Fraendberg P.-A., Ahlner J., Axelsson K.L., Wikberg J.E.S. FEBS Lett. 290:157-158(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 337-545, ALTERNATIVE SPLICING. Tissue: Lung.
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

X66533 mRNA. Translation: CAA47144.1.
AF020340 mRNA. Translation: AAB94877.1.
BC047620 mRNA. Translation: AAH47620.2.

IPI

IPI00215651.
IPI00289033.

PIR

S23097.

RefSeq

NP_000848.1. NM_000857.2.

UniGene

Hs.77890.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WZ1	X-ray	1.63	A/B	408-619	[»]
3UVJ	X-ray	2.08	B/D	408-619	[»]

ProteinModelPortal

Q02153.

ModBase

Search...

STRING

9606.ENSP00000264424.

PhosphoSite

Q02153.

DMDM

399328.

PaxDb

Q02153.

PRIDE

Q02153.

StructuralBiologyKnowledgebase

Search...

Ensembl

ENST00000264424; ENSP00000264424; ENSG00000061918.
ENST00000503520; ENSP00000420842; ENSG00000061918.

GeneID

2983.

KEGG

hsa:2983.

UCSC

uc003ipc.3. human.
uc010iqf.3. human.

CTD

2983.

GeneCards

GC04P156680.

HGNC

HGNC:4687. GUCY1B3.

HPA

CAB010890.
HPA020870.

MIM

139397. gene.

neXtProt

NX_Q02153.

PharmGKB

PA29068.

GenAtlas

Search...

eggNOG

COG2114.

HOGENOM

HOG000220903.

HOVERGEN

HBG051715.

InParanoid

Q02153.

K12319.

OrthoDB

EOG41RPTG.

Reactome

REACT_604. Hemostasis.

ArrayExpress

Q02153.

Bgee

Q02153.

CleanEx

HS_GUCY1B3.

Genevestigator

Q02153.

GermOnline

ENSG00000061918. Homo sapiens.

Gene3D

3.30.70.1230. 1 hit.

InterPro

IPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011645. Haem_no_assoc-bd.
IPR011644. Heme_NO-bd.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
[Graphical view]

Pfam

PF00211. Guanylate_cyc. 1 hit.
PF07700. HNOB. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]

SMART

SM00044. CYCc. 1 hit.
[Graphical view]

SUPFAM

SSF55073. A/G_cyclase. 1 hit.
SSF111126. Haem_NO-bd. 1 hit.

PROSITE

PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

BindingDB

Q02153.

ChEMBL

CHEMBL2046.

EvolutionaryTrace

Q02153.

GenomeRNAi

2983.

NextBio

11832.

SOURCE

Search...

Entry name

GCYB1_HUMAN

Accession

Primary (citable) accession number: Q02153
Secondary accession number(s): Q86WY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 128 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform HSGC-1 (identifier: Q02153-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform HSGC-2 (identifier: Q02153-2)

The sequence of this isoform differs from the canonical sequence as follows:
393-425: Missing.