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Q02153 (GCYB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanylate cyclase soluble subunit beta-1

Short name=GCS-beta-1
EC=4.6.1.2
Alternative name(s):
Guanylate cyclase soluble subunit beta-3
Short name=GCS-beta-3
Soluble guanylate cyclase small subunit
Gene names
Name:GUCY1B3
Synonyms:GUC1B3, GUCSB3, GUCY1B1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length619 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

GTP = 3',5'-cyclic GMP + diphosphate.

Cofactor

Binds 1 or 2 heme groups per heterodimer By similarity.

Enzyme regulation

Activated by nitric oxide in the presence of magnesium or manganese ions.

Subunit structure

Heterodimer of an alpha and a beta chain.

Subcellular location

Cytoplasm.

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Sequence similarities

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Contains 1 guanylate cyclase domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform HSGC-1 (identifier: Q02153-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform HSGC-2 (identifier: Q02153-2)

The sequence of this isoform differs from the canonical sequence as follows:
     393-425: Missing.
Isoform 3 (identifier: Q02153-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: MYGFVNHALELLVIRNYGPEVWEDIK → MLMCFI
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 619619Guanylate cyclase soluble subunit beta-1
PRO_0000074116

Regions

Domain421 – 554134Guanylate cyclase

Sites

Metal binding1051Iron (heme proximal ligand) By similarity

Natural variations

Alternative sequence1 – 2626MYGFV…WEDIK → MLMCFI in isoform 3.
VSP_054365
Alternative sequence393 – 42533Missing in isoform HSGC-2.
VSP_001813

Secondary structure

............................... 619
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform HSGC-1 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 231E4E660DE02AA1

FASTA61970,514
        10         20         30         40         50         60 
MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD LVAAASKVLN 

        70         80         90        100        110        120 
LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN LDALHDHLAT IYPGMRAPSF 

       130        140        150        160        170        180 
RCTDAEKGKG LILHYYSERE GLQDIVIGII KTVAQQIHGT EIDMKVIQQR NEECDHTQFL 

       190        200        210        220        230        240 
IEEKESKEED FYEDLDRFEE NGTQESRISP YTFCKAFPFH IIFDRDLVVT QCGNAIYRVL 

       250        260        270        280        290        300 
PQLQPGNCSL LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKL ECEDELTGTE 

       310        320        330        340        350        360 
ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR DLVLLGEQFR 

       370        380        390        400        410        420 
EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTLLYSVLPP SVANELRHKR PVPAKRYDNV 

       430        440        450        460        470        480 
TILFSGIVGF NAFCSKHASG EGAMKIVNLL NDLYTRFDTL TDSRKNPFVY KVETVGDKYM 

       490        500        510        520        530        540 
TVSGLPEPCI HHARSICHLA LDMMEIAGQV QVDGESVQIT IGIHTGEVVT GVIGQRMPRY 

       550        560        570        580        590        600 
CLFGNTVNLT SRTETTGEKG KINVSEYTYR CLMSPENSDP QFHLEHRGPV SMKGKKEPMQ 

       610 
VWFLSRKNTG TEETKQDDD 

« Hide

Isoform HSGC-2 [UniParc].

Checksum: 59818154086FD4A3
Show »

FASTA58666,738
Isoform 3 [UniParc].

Checksum: 93F6129D51BECB23
Show »

FASTA59968,165

References

« Hide 'large scale' references
[1]"Molecular cloning of the cDNAs coding for the two subunits of soluble guanylyl cyclase from human brain."
Giuili G., Scholl U., Bulle F., Guellaeen G.
FEBS Lett. 304:83-88(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]Gansemans Y., Brouckaert P., Fiers W.
Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM HSGC-2).
Tissue: Kidney.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Tongue.
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM HSGC-1).
Tissue: Brain.
[6]"Heterogeneity in human soluble guanylate cyclase due to alternative splicing."
Chhajilani V., Fraendberg P.-A., Ahlner J., Axelsson K.L., Wikberg J.E.S.
FEBS Lett. 290:157-158(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 337-545, ALTERNATIVE SPLICING.
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X66533 mRNA. Translation: CAA47144.1.
AF020340 mRNA. Translation: AAB94877.1.
AK296680 mRNA. Translation: BAH12412.1.
AC114761 Genomic DNA. No translation available.
BC047620 mRNA. Translation: AAH47620.2.
CCDSCCDS47154.1. [Q02153-1]
PIRS23097.
RefSeqNP_000848.1. NM_000857.3. [Q02153-1]
UniGeneHs.77890.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WZ1X-ray1.63A/B408-619[»]
3UVJX-ray2.08B/D408-619[»]
4NI2X-ray1.90B408-608[»]
ProteinModelPortalQ02153.
SMRQ02153. Positions 1-182, 212-334, 348-408, 412-610.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109238. 6 interactions.
IntActQ02153. 1 interaction.
STRING9606.ENSP00000264424.

Chemistry

BindingDBQ02153.
ChEMBLCHEMBL2111348.

PTM databases

PhosphoSiteQ02153.

Polymorphism databases

DMDM399328.

Proteomic databases

MaxQBQ02153.
PaxDbQ02153.
PRIDEQ02153.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264424; ENSP00000264424; ENSG00000061918. [Q02153-1]
ENST00000503520; ENSP00000420842; ENSG00000061918. [Q02153-2]
ENST00000505764; ENSP00000426319; ENSG00000061918.
GeneID2983.
KEGGhsa:2983.
UCSCuc003ipc.3. human. [Q02153-1]
uc010iqf.3. human. [Q02153-2]

Organism-specific databases

CTD2983.
GeneCardsGC04P156680.
HGNCHGNC:4687. GUCY1B3.
HPACAB010890.
HPA020870.
MIM139397. gene.
neXtProtNX_Q02153.
PharmGKBPA29068.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2114.
HOGENOMHOG000220903.
HOVERGENHBG051715.
InParanoidQ02153.
KOK12319.
OrthoDBEOG7BS48T.
PhylomeDBQ02153.
TreeFamTF351403.

Enzyme and pathway databases

ReactomeREACT_604. Hemostasis.

Gene expression databases

ArrayExpressQ02153.
BgeeQ02153.
CleanExHS_GUCY1B3.
GenevestigatorQ02153.

Family and domain databases

Gene3D3.30.70.1230. 1 hit.
InterProIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011645. Haem_no_assoc-bd.
IPR011644. Heme_NO-bd.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
[Graphical view]
PfamPF00211. Guanylate_cyc. 1 hit.
PF07700. HNOB. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]
SMARTSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ02153.
GeneWikiGUCY1B3.
GenomeRNAi2983.
NextBio11832.
PROQ02153.
SOURCESearch...

Entry information

Entry nameGCYB1_HUMAN
AccessionPrimary (citable) accession number: Q02153
Secondary accession number(s): B7Z426, Q86WY5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 9, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM