Reviewed, UniProtKB/Swiss-Prot Q02152 (5HT2B_MOUSE)

Names and origin

Protein names

Recommended name:
    5-hydroxytryptamine receptor 2B
      Short name=5-HT-2B
Alternative name(s):
    5-HT-2F
    Serotonin receptor 2B
    NP75 protein

Gene names

Name:

Htr2b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subunit structure	Interacts with MPDZ By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Intestine and heart, but also in brain and kidney.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence CAA78824.1 differs from that shown. Reason: Erroneous termination at position 480. Translated as stop.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Gene Ontology (GO)
Biological process	activation of phospholipase C activity by serotonin receptor signaling pathway Traceable author statement. Source: MGI heart development Inferred from mutant phenotype. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serotonin receptor activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	5-hydroxytryptamine receptor 2B		PRO_0000068954
Regions
	Topological domain	1 – 55	55	Extracellular By similarity
	Transmembrane	56 – 78	23	1 By similarity
	Topological domain	79 – 90	12	Cytoplasmic By similarity
	Transmembrane	91 – 112	22	2 By similarity
	Topological domain	113 – 128	16	Extracellular By similarity
	Transmembrane	129 – 150	22	3 By similarity
	Topological domain	151 – 170	20	Cytoplasmic By similarity
	Transmembrane	171 – 191	21	4 By similarity
	Topological domain	192 – 215	24	Extracellular By similarity
	Transmembrane	216 – 238	23	5 By similarity
	Topological domain	239 – 323	85	Cytoplasmic By similarity
	Transmembrane	324 – 344	21	6 By similarity
	Topological domain	345 – 359	15	Extracellular By similarity
	Transmembrane	360 – 382	23	7 By similarity
	Topological domain	383 – 479	97	Cytoplasmic By similarity
	Motif	477 – 479	3	PDZ-binding
Amino acid modifications
	Lipidation	396	1	S-palmitoyl cysteine Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Disulfide bond	127 ↔ 206		By similarity
Experimental info
	Sequence conflict	167	1	S → T in CAA78824. Ref.1
	Sequence conflict	227	1	V → A Ref.3
	Sequence conflict	227	1	V → A Ref.4
	Sequence conflict	227	1	V → A Ref.5
	Sequence conflict	449	1	S → C in CAA78824. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q02152-1 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: FDB030D809079B6D	FASTA	479	53,625
	10 20 30 40 50 60 MASSYKMSEQ STTSEHILQK TCDHLILTNR SGLETDSVAE EMKQTVEGQG HTVHWAALLI 70 80 90 100 110 120 LAVIIPTIGG NILVILAVAL EKRLQYATNY FLMSLAIADL LVGLFVMPIA LLTIMFEAIW 130 140 150 160 170 180 PLPLALCPAW LFLDVLFSTA SIMHLCAISL DRYIAIKKPI QANQCNSRAT AFIKITVVWL 190 200 210 220 230 240 ISIGIAIPVP IKGIETDVIN PHNVTCELTK DRFGSFMVFG SLAAFFVPLT IMVVTYFLTI 250 260 270 280 290 300 HTLQKKAYLV KNKPPQRLTR WTVPTVFLRE DSSFSSPEKV AMLDGSHRDK ILPNSSDETL 310 320 330 340 350 360 MRRMSSVGKR SAQTISNEQR ASKALGVVFF LFLLMWCPFF ITNLTLALCD SCNQTTLKTL 370 380 390 400 410 420 LEIFVWIGYV SSGVNPLIYT LFNKTFREAF GRYITCNYRA TKSVKALRKF SSTLCFGNSM 430 440 450 460 470 VENSKFFTKH GIRNGINPAM YQSPMRLRSS TIQSSSIILL DTLLTENDGD KAEEQVSYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New mouse 5-HT2-like receptor. Expression in brain, heart and intestine." Loric S., Launay J.-M., Colas J.-F., Maroteaux L. FEBS Lett. 312:203-207(1992) [PubMed: 1426253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Genomic sequence of the 5-HT2B receptor locus." Choi D.S., Maroteaux L. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[3]	"High-throughput sequence identification of gene coding variants within alcohol-related QTLs." Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M. Mamm. Genome 12:657-663(2001) [PubMed: 11471062] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ILS and ISS.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cecum.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z15119 mRNA. Translation: CAA78824.1. Sequence problems. AJ012488 Genomic DNA. Translation: CAA10051.1. AF498254 mRNA. Translation: AAM22971.1. AF498255 mRNA. Translation: AAM22972.1. AK033713 mRNA. Translation: BAC28441.1. BC023690 mRNA. Translation: AAH23690.1.
IPI	IPI00124615.
PIR	S27269.
RefSeq	NP_032337.2.
UniGene	Mm.439747
3D structure databases
HSSP	HSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSMUSG00000026228. Mus musculus. [Contig view]
GeneID	15559.
KEGG	mmu:15559.
Organism-specific databases
MGI	MGI:109323. Htr2b.
Phylogenomic databases
HOGENOM	Q02152.
HOVERGEN	Q02152.
Gene expression databases
ArrayExpress	Q02152.
Bgee	Q02152.
CleanEx	MM_HTR2B.
GermOnline	ENSMUSG00000026228. Mus musculus.
Family and domain databases
InterPro	IPR000482. 5HT2B_rcpt. IPR002231. 5HT_rcpt. IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00651. 5HT2BRECEPTR. PR01101. 5HTRECEPTOR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	288504.
SOURCE	Search...

Entry information

Entry name

5HT2B_MOUSE

Accession

Primary (citable) accession number: Q02152
Secondary accession number(s): Q8JZK5, Q9QWS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	June 21, 2005
Last modified:	June 16, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)