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Protein

Histidinol-phosphatase

Gene

hisK

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-histidinol phosphate + H2O = L-histidinol + phosphate.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 8 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase regulatory subunit (hisZ)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
  6. Imidazoleglycerol-phosphate dehydratase (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. Histidinol-phosphatase (hisK)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00031; UER00013.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidinol-phosphatase (EC:3.1.3.15)
Short name:
HolPase
Gene namesi
Name:hisK
Ordered Locus Names:LL1216
ORF Names:L37351
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001223191 – 269Histidinol-phosphataseAdd BLAST269

Proteomic databases

PaxDbiQ02150.

Interactioni

Protein-protein interaction databases

STRINGi272623.L37351.

Structurei

Secondary structure

1269
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 29Combined sources10
Beta strandi33 – 42Combined sources10
Helixi54 – 67Combined sources14
Turni68 – 71Combined sources4
Beta strandi73 – 81Combined sources9
Helixi84 – 86Combined sources3
Helixi87 – 94Combined sources8
Beta strandi100 – 105Combined sources6
Helixi119 – 121Combined sources3
Helixi126 – 143Combined sources18
Beta strandi148 – 150Combined sources3
Helixi155 – 158Combined sources4
Beta strandi159 – 161Combined sources3
Helixi170 – 172Combined sources3
Helixi173 – 185Combined sources13
Beta strandi189 – 193Combined sources5
Turni195 – 198Combined sources4
Helixi200 – 215Combined sources16
Beta strandi220 – 224Combined sources5
Beta strandi228 – 230Combined sources3
Helixi234 – 246Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GC3X-ray1.32A2-269[»]
4GK8X-ray1.93A2-269[»]
4GYFX-ray1.65A2-269[»]
ProteinModelPortaliQ02150.
SMRiQ02150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PHP hydrolase family. HisK subfamily.Curated

Phylogenomic databases

eggNOGiENOG4107RVV. Bacteria.
COG1387. LUCA.
HOGENOMiHOG000244025.
KOiK04486.
OMAiYHIHSNF.

Family and domain databases

InterProiIPR010140. Histidinol_P_phosphatase_HisJ.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
[Graphical view]
PANTHERiPTHR21039. PTHR21039. 1 hit.
PfamiPF02811. PHP. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR01856. hisJ_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02150-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLDYHFHS HFSADSEELP RKHVTEAIAH GLEEICFTEH RDFYFPGMDF
60 70 80 90 100
SLNLPEYFQE INRLQAEFKD KIKIKIGLEM GIDLRFKSEI NQFIDSAPFD
110 120 130 140 150
FVIASVHEIG DIEVYDGTEF YLQKIKEEAQ REYLLACLDV VQNFENYNSF
160 170 180 190 200
GHLDYVARYG PYTDKSIKFA ENREILFEIL RALASKEKAL EINTRLFDDP
210 220 230 240 250
KTEQFYSDLL INFKKLGGKF ITLGTDSHIA KRDWLSIHKA RTLIKKAGFH
260
ELATFSGMKI DKNKKSIKE
Length:269
Mass (Da):31,473
Last modified:September 26, 2001 - v2
Checksum:iCF51601DEDD5B8C2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25T → I in AAB81911 (PubMed:1400209).Curated1
Sequence conflicti30H → Y in AAB81911 (PubMed:1400209).Curated1
Sequence conflicti71 – 73KIK → EIN in AAB81911 (PubMed:1400209).Curated3
Sequence conflicti125I → T in AAB81911 (PubMed:1400209).Curated1
Sequence conflicti146N → T in AAB81911 (PubMed:1400209).Curated1
Sequence conflicti168K → T in AAB81911 (PubMed:1400209).Curated1
Sequence conflicti187E → G in AAB81911 (PubMed:1400209).Curated1
Sequence conflicti203E → G in AAB81911 (PubMed:1400209).Curated1
Sequence conflicti215K → R in AAB81911 (PubMed:1400209).Curated1
Sequence conflicti250H → R in AAB81911 (PubMed:1400209).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92974 Genomic DNA. Translation: AAB81911.1.
AE005176 Genomic DNA. Translation: AAK05314.1.
PIRiD47754.
H86776.
RefSeqiNP_267372.1. NC_002662.1.
WP_003131121.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05314; AAK05314; L37351.
GeneIDi1114864.
KEGGilla:L37351.
PATRICi22294810. VBILacLac136773_1313.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92974 Genomic DNA. Translation: AAB81911.1.
AE005176 Genomic DNA. Translation: AAK05314.1.
PIRiD47754.
H86776.
RefSeqiNP_267372.1. NC_002662.1.
WP_003131121.1. NC_002662.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GC3X-ray1.32A2-269[»]
4GK8X-ray1.93A2-269[»]
4GYFX-ray1.65A2-269[»]
ProteinModelPortaliQ02150.
SMRiQ02150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L37351.

Proteomic databases

PaxDbiQ02150.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05314; AAK05314; L37351.
GeneIDi1114864.
KEGGilla:L37351.
PATRICi22294810. VBILacLac136773_1313.

Phylogenomic databases

eggNOGiENOG4107RVV. Bacteria.
COG1387. LUCA.
HOGENOMiHOG000244025.
KOiK04486.
OMAiYHIHSNF.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00013.

Family and domain databases

InterProiIPR010140. Histidinol_P_phosphatase_HisJ.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
[Graphical view]
PANTHERiPTHR21039. PTHR21039. 1 hit.
PfamiPF02811. PHP. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR01856. hisJ_fam. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHIS9_LACLA
AccessioniPrimary (citable) accession number: Q02150
Secondary accession number(s): Q9CG89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: September 26, 2001
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.