Q02150 (HIS9_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidinol-phosphatase
Short name=HolPase
EC=3.1.3.15

Gene names

Name:	hisK
Ordered Locus Names:	LL1216
ORF Names:	L37351

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. lactis

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-histidinol phosphate + H2O = L-histidinol + phosphate.
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.
Sequence similarities	Belongs to the PHP hydrolase family. HisK subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: InterPro histidinol-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 269

269

Histidinol-phosphatase

PRO_0000122319

Experimental info

Sequence conflict

25

1

T → I in AAB81911. Ref.1

Sequence conflict

30

1

H → Y in AAB81911. Ref.1

Sequence conflict

71 – 73

3

KIK → EIN in AAB81911. Ref.1

Sequence conflict

125

1

I → T in AAB81911. Ref.1

Sequence conflict

146

1

N → T in AAB81911. Ref.1

Sequence conflict

168

1

K → T in AAB81911. Ref.1

Sequence conflict

187

1

E → G in AAB81911. Ref.1

Sequence conflict

203

1

E → G in AAB81911. Ref.1

Sequence conflict

215

1

K → R in AAB81911. Ref.1

Sequence conflict

250

1

H → R in AAB81911. Ref.1

Secondary structure

1

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269

Helix Strand Turn

Details...

Helix

20 – 30

11

Beta strand

33 – 42

10

Helix

54 – 67

14

Turn

68 – 71

4

Beta strand

73 – 81

9

Helix

84 – 86

3

Helix

87 – 95

9

Beta strand

100 – 105

6

Helix

119 – 121

3

Helix

126 – 143

18

Beta strand

148 – 150

3

Helix

155 – 158

4

Beta strand

159 – 161

3

Helix

170 – 172

3

Helix

173 – 185

13

Beta strand

189 – 193

5

Turn

195 – 198

4

Helix

200 – 215

16

Beta strand

221 – 224

4

Beta strand

227 – 230

4

Helix

234 – 246

13

Sequences

	Sequence		Length	Mass (Da)
	Q02150 [UniParc]. Last modified September 26, 2001. Version 2. Checksum: CF51601DEDD5B8C2	FASTA	269	31,473
	10 20 30 40 50 60 MKKLDYHFHS HFSADSEELP RKHVTEAIAH GLEEICFTEH RDFYFPGMDF SLNLPEYFQE 70 80 90 100 110 120 INRLQAEFKD KIKIKIGLEM GIDLRFKSEI NQFIDSAPFD FVIASVHEIG DIEVYDGTEF 130 140 150 160 170 180 YLQKIKEEAQ REYLLACLDV VQNFENYNSF GHLDYVARYG PYTDKSIKFA ENREILFEIL 190 200 210 220 230 240 RALASKEKAL EINTRLFDDP KTEQFYSDLL INFKKLGGKF ITLGTDSHIA KRDWLSIHKA 250 260 RTLIKKAGFH ELATFSGMKI DKNKKSIKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Histidine biosynthesis genes in Lactococcus lactis subsp. lactis." Delorme C., Ehrlich S.D., Renault P. J. Bacteriol. 174:6571-6579(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCDO 2118.
[2]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U92974 Genomic DNA. Translation: AAB81911.1.
AE005176 Genomic DNA. Translation: AAK05314.1.

PIR

D47754.
H86776.

RefSeq

NP_267372.1. NC_002662.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GC3	X-ray	1.32	A	2-269	[»]
4GK8	X-ray	1.93	A	2-269	[»]
4GYF	X-ray	1.65	A	2-269	[»]

ProteinModelPortal

Q02150.

ModBase

Search...

Protein-protein interaction databases

STRING

272623.L37351.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK05314; AAK05314; L37351.

GeneID

1114864.

KEGG

lla:L37351.

PATRIC

22294810. VBILacLac136773_1313.

Phylogenomic databases

eggNOG

COG1387.

HOGENOM

HOG000244025.

KO

K04486.

OMA

LDYPARY.

ProtClustDB

CLSK697637.

Enzyme and pathway databases

BioCyc

LLAC272623:GHSH-1345-MONOMER.

UniPathway

UPA00031; UER00013.

Family and domain databases

InterPro

IPR010140. Histidinol_P_phosphatase_HisJ.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
[Graphical view]

PANTHER

PTHR21039. PTHR21039. 1 hit.

Pfam

PF02811. PHP. 1 hit.
[Graphical view]

SMART

SM00481. POLIIIAc. 1 hit.
[Graphical view]

SUPFAM

SSF89550. PHP-like. 1 hit.

TIGRFAMs

TIGR01856. hisJ_fam. 1 hit.

ProtoNet

Search...

Entry information

Entry name

HIS9_LACLA

Accession

Primary (citable) accession number: Q02150
Secondary accession number(s): Q9CG89

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	September 26, 2001
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program