Q02137 (ILVB_LACLA) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 95.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Acetolactate synthase large subunit Short name=AHAS EC=2.2.1.6 Alternative name(s): Acetohydroxy-acid synthase large subunit Short name=ALS | ||||||
| Gene names |
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| Organism | Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) | ||||||
| Taxonomic identifier | 272623 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus |
Protein attributes
| Sequence length | 575 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | 2 pyruvate = 2-acetolactate + CO2. |
| Cofactor | Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity. |
| Pathway | Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4. |
| Subunit structure | Dimer of large and small chains. |
| Miscellaneous | Contains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry By similarity. |
| Sequence similarities | Belongs to the TPP enzyme family. |
| Sequence caution | The sequence AAK05322.1 differs from that shown. Reason: Erroneous termination at position 5. Translated as Lys. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Branched-chain amino acid biosynthesis |
| Ligand | FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate |
| Molecular function | Transferase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | acetolactate synthase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide bindingInferred from electronic annotation. Source: InterPro magnesium ion bindingInferred from electronic annotation. Source: InterPro thiamine pyrophosphate bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 575 | 575 | Acetolactate synthase large subunit | PRO_0000090785 | |||||
Regions | |||||||||
| Nucleotide binding | 265 – 286 | 22 | FAD By similarity | ||||||
| Nucleotide binding | 308 – 327 | 20 | FAD By similarity | ||||||
| Region | 395 – 475 | 81 | Thiamine pyrophosphate binding | ||||||
Sites | |||||||||
| Metal binding | 446 | 1 | Magnesium By similarity | ||||||
| Metal binding | 473 | 1 | Magnesium By similarity | ||||||
| Binding site | 57 | 1 | Thiamine pyrophosphate By similarity | ||||||
| Binding site | 159 | 1 | FAD By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 111 – 112 | 2 | PL → RQ in AAB81919. Ref.1 | ||||||
| Sequence conflict | 298 | 1 | V → F in AAB81919. Ref.1 | ||||||
| Sequence conflict | 353 – 354 | 2 | TK → IE in AAB81919. Ref.1 | ||||||
| Sequence conflict | 557 | 1 | S → N in AAB81919. Ref.1 | ||||||
| Sequence conflict | 568 | 1 | E → K in AAB81919. Ref.1 | ||||||
| Sequence conflict | 572 | 1 | V → I in AAB81919. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp. lactis." Godon J.-J., Chopin M.-C., Ehrlich S.D. J. Bacteriol. 174:6580-6589(1992) [PubMed: 1400210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCDO 2118. |
| [2] | "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U92974 Genomic DNA. Translation: AAB81919.1. AE005176 Genomic DNA. Translation: AAK05322.1. Sequence problems. |
| PIR | H86777. S35138. |
| RefSeq | NP_267380.1. NC_002662.1. |
3D structure databases | |
| ProteinModelPortal | Q02137. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1114873. |
| GenomeReviews | Gene locus LL1224 in contig AE005176_GR. |
| KEGG | lla:L0078. |
| NMPDR | fig|272623.1.peg.1259. |
| PATRIC | 22294830. VBILacLac136773_1323. |
Phylogenomic databases | |
| HOGENOM | HBG323037. |
| ProtClustDB | PRK07282. |
Enzyme and pathway databases | |
| BioCyc | LLAC272623:L0078-MONOMER. |
Family and domain databases | |
| InterPro | IPR012846. Acetolactate_synth_lsu. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view] |
| KO | K01652. |
| PANTHER | PTHR18968:SF13. PTHR18968:SF13. 1 hit. |
| Pfam | PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00118. Acolac_lg. 1 hit. |
| PROSITE | PS00187. TPP_ENZYMES. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ILVB_LACLA | ||||||||
| Accession | Primary (citable) accession number: Q02137 Secondary accession number(s): Q9CG84 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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