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Protein

Histidinol dehydrogenase

Gene

hisD

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.By similarity

Catalytic activityi

L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei129 – 1291NADBy similarity
Binding sitei191 – 1911NADBy similarity
Binding sitei214 – 2141NADBy similarity
Binding sitei237 – 2371SubstrateBy similarity
Metal bindingi259 – 2591ZincBy similarity
Binding sitei259 – 2591SubstrateBy similarity
Metal bindingi262 – 2621ZincBy similarity
Binding sitei262 – 2621SubstrateBy similarity
Active sitei327 – 3271Proton acceptorBy similarity
Active sitei328 – 3281Proton acceptorBy similarity
Binding sitei328 – 3281SubstrateBy similarity
Metal bindingi361 – 3611ZincBy similarity
Binding sitei361 – 3611SubstrateBy similarity
Binding sitei415 – 4151SubstrateBy similarity
Metal bindingi420 – 4201ZincBy similarity
Binding sitei420 – 4201SubstrateBy similarity

GO - Molecular functioni

  1. histidinol dehydrogenase activity Source: UniProtKB-HAMAP
  2. NAD binding Source: InterPro
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. histidine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1296-MONOMER.
UniPathwayiUPA00031; UER00014.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidinol dehydrogenase (EC:1.1.1.23)
Short name:
HDH
Gene namesi
Name:hisD
Ordered Locus Names:LL1209
ORF Names:L0067
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431Histidinol dehydrogenasePRO_0000135783Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272623.L0067.

Structurei

3D structure databases

ProteinModelPortaliQ02136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidinol dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG0141.
HOGENOMiHOG000243914.
KOiK00013.
OrthoDBiEOG6CVVCR.

Family and domain databases

HAMAPiMF_01024. HisD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR001692. Histidinol_DH_CS.
IPR022695. Histidinol_DH_monofunct.
IPR012131. Hstdl_DH.
[Graphical view]
PfamiPF00815. Histidinol_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000099. Histidinol_dh. 1 hit.
PRINTSiPR00083. HOLDHDRGNASE.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00069. hisD. 1 hit.
PROSITEiPS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q02136-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKQIDYQGK LEEIAEKFQG RKTEVSKEVN KTVQQIVADI QKNGDTALFN
60 70 80 90 100
YAKKFDGYDV NTSNLLVTRM EREAGLEQID EDYFRILRRT KSQIEEFHKH
110 120 130 140 150
QLGNSWNIFK ENGVIMGQIA RPLERVALYV PGGTAAYPST VIMNAVPALL
160 170 180 190 200
AGVKEIIMIT PVKADGKVNP NILAAAEVCG IETIYKVGGA QGVAAVAYGT
210 220 230 240 250
ESIPKVDKIV GPGNIFVATA KKICYGVVDI DMIAGPSEVL VIADKTAKPK
260 270 280 290 300
YIAADLMAQA EHDKLASAIL VTTSEKLVQQ VDEELNRQVQ NLERREIIES
310 320 330 340 350
SIRNYGGAIV VKNIDDAFDV SNQLAPEHLE VLTSEPLTQL PKIKNAGSIF
360 370 380 390 400
IGEYTPEPLG DYMSGSNHVL PTGGTAKFYS GLGVYNFIKY LTYSYYPKEV
410 420 430
LADFKEDVET FAKSEGLTAH ANSISVRFDE M
Length:431
Mass (Da):47,411
Last modified:January 3, 2005 - v3
Checksum:i3FFD2B5C75F9D1B0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251V → L in AAK05307 (PubMed:11337471).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti38 – 381A → E in strain: IL1403.
Natural varianti43 – 431N → S in strain: IL1403.
Natural varianti69 – 691R → P in strain: IL1403.
Natural varianti74 – 741A → T in strain: IL1403.
Natural varianti164 – 1641A → T in strain: IL1403.
Natural varianti245 – 2451K → E in strain: IL1403.
Natural varianti248 – 2481K → N in strain: IL1403.
Natural varianti295 – 2951R → S in strain: IL1403.
Natural varianti301 – 3033SIR → YIS in strain: IL1403.
Natural varianti316 – 3161D → E in strain: IL1403.
Natural varianti403 – 4031D → A in strain: IL1403.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92974 Genomic DNA. No translation available.
AE005176 Genomic DNA. Translation: AAK05307.1.
PIRiA86776.
E45734.
RefSeqiNP_267365.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05307; AAK05307; L0067.
GeneIDi1114856.
KEGGilla:L0067.
PATRICi22294792. VBILacLac136773_1304.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92974 Genomic DNA. No translation available.
AE005176 Genomic DNA. Translation: AAK05307.1.
PIRiA86776.
E45734.
RefSeqiNP_267365.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ02136.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L0067.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05307; AAK05307; L0067.
GeneIDi1114856.
KEGGilla:L0067.
PATRICi22294792. VBILacLac136773_1304.

Phylogenomic databases

eggNOGiCOG0141.
HOGENOMiHOG000243914.
KOiK00013.
OrthoDBiEOG6CVVCR.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00014.
BioCyciLLAC272623:GHSH-1296-MONOMER.

Family and domain databases

HAMAPiMF_01024. HisD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR001692. Histidinol_DH_CS.
IPR022695. Histidinol_DH_monofunct.
IPR012131. Hstdl_DH.
[Graphical view]
PfamiPF00815. Histidinol_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000099. Histidinol_dh. 1 hit.
PRINTSiPR00083. HOLDHDRGNASE.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00069. hisD. 1 hit.
PROSITEiPS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Histidine biosynthesis genes in Lactococcus lactis subsp. lactis."
    Delorme C., Ehrlich S.D., Renault P.
    J. Bacteriol. 174:6571-6579(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCDO 2118.
  2. "Gene inactivation in Lactococcus lactis: histidine biosynthesis."
    Delorme C., Godon J.-J., Ehrlich S.D., Renault P.
    J. Bacteriol. 175:4391-4399(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: IL1403.
  3. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiHISX_LACLA
AccessioniPrimary (citable) accession number: Q02136
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1993
Last sequence update: January 3, 2005
Last modified: January 6, 2015
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

This protein is inactive in the dairy strain IL1403. The histidine biosynthesis pathway is not functional in the dairy strain IL1403.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.