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Q02136 (HISX_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histidinol dehydrogenase

Short name=HDH
EC=1.1.1.23
Gene names
Name:hisD
Ordered Locus Names:LL1209
ORF Names:L0067
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length431 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine By similarity. HAMAP-Rule MF_01024

Catalytic activity

L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH. HAMAP-Rule MF_01024

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01024

Pathway

Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9. HAMAP-Rule MF_01024

Sequence similarities

Belongs to the histidinol dehydrogenase family.

Caution

This protein is inactive in the dairy strain IL1403. The histidine biosynthesis pathway is not functional in the dairy strain IL1403.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Histidine biosynthesis
   LigandMetal-binding
NAD
Zinc
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processhistidine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionNAD binding

Inferred from electronic annotation. Source: InterPro

histidinol dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 431431Histidinol dehydrogenase HAMAP-Rule MF_01024
PRO_0000135783

Sites

Active site3271Proton acceptor By similarity
Active site3281Proton acceptor By similarity
Metal binding2591Zinc By similarity
Metal binding2621Zinc By similarity
Metal binding3611Zinc By similarity
Metal binding4201Zinc By similarity
Binding site1291NAD By similarity
Binding site1911NAD By similarity
Binding site2141NAD By similarity
Binding site2371Substrate By similarity
Binding site2591Substrate By similarity
Binding site2621Substrate By similarity
Binding site3281Substrate By similarity
Binding site3611Substrate By similarity
Binding site4151Substrate By similarity
Binding site4201Substrate By similarity

Natural variations

Natural variant381A → E in strain: IL1403.
Natural variant431N → S in strain: IL1403.
Natural variant691R → P in strain: IL1403.
Natural variant741A → T in strain: IL1403.
Natural variant1641A → T in strain: IL1403.
Natural variant2451K → E in strain: IL1403.
Natural variant2481K → N in strain: IL1403.
Natural variant2951R → S in strain: IL1403.
Natural variant301 – 3033SIR → YIS in strain: IL1403.
Natural variant3161D → E in strain: IL1403.
Natural variant4031D → A in strain: IL1403.

Experimental info

Sequence conflict251V → L in AAK05307. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q02136 [UniParc].

Last modified January 4, 2005. Version 3.
Checksum: 3FFD2B5C75F9D1B0

FASTA43147,411
        10         20         30         40         50         60 
MLKQIDYQGK LEEIAEKFQG RKTEVSKEVN KTVQQIVADI QKNGDTALFN YAKKFDGYDV 

        70         80         90        100        110        120 
NTSNLLVTRM EREAGLEQID EDYFRILRRT KSQIEEFHKH QLGNSWNIFK ENGVIMGQIA 

       130        140        150        160        170        180 
RPLERVALYV PGGTAAYPST VIMNAVPALL AGVKEIIMIT PVKADGKVNP NILAAAEVCG 

       190        200        210        220        230        240 
IETIYKVGGA QGVAAVAYGT ESIPKVDKIV GPGNIFVATA KKICYGVVDI DMIAGPSEVL 

       250        260        270        280        290        300 
VIADKTAKPK YIAADLMAQA EHDKLASAIL VTTSEKLVQQ VDEELNRQVQ NLERREIIES 

       310        320        330        340        350        360 
SIRNYGGAIV VKNIDDAFDV SNQLAPEHLE VLTSEPLTQL PKIKNAGSIF IGEYTPEPLG 

       370        380        390        400        410        420 
DYMSGSNHVL PTGGTAKFYS GLGVYNFIKY LTYSYYPKEV LADFKEDVET FAKSEGLTAH 

       430 
ANSISVRFDE M 

« Hide

References

« Hide 'large scale' references
[1]"Histidine biosynthesis genes in Lactococcus lactis subsp. lactis."
Delorme C., Ehrlich S.D., Renault P.
J. Bacteriol. 174:6571-6579(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCDO 2118.
[2]"Gene inactivation in Lactococcus lactis: histidine biosynthesis."
Delorme C., Godon J.-J., Ehrlich S.D., Renault P.
J. Bacteriol. 175:4391-4399(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: IL1403.
[3]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U92974 Genomic DNA. No translation available.
AE005176 Genomic DNA. Translation: AAK05307.1.
PIRA86776.
E45734.
RefSeqNP_267365.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ02136.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L0067.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK05307; AAK05307; L0067.
GeneID1114856.
KEGGlla:L0067.
PATRIC22294792. VBILacLac136773_1304.

Phylogenomic databases

eggNOGCOG0141.
HOGENOMHOG000243914.
KOK00013.
OrthoDBEOG6CVVCR.
ProtClustDBCLSK697630.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-1296-MONOMER.
UniPathwayUPA00031; UER00014.

Family and domain databases

HAMAPMF_01024. HisD.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR001692. Histidinol_DH_CS.
IPR022695. Histidinol_DH_monofunct.
IPR012131. Hstdl_DH.
[Graphical view]
PfamPF00815. Histidinol_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000099. Histidinol_dh. 1 hit.
PRINTSPR00083. HOLDHDRGNASE.
SUPFAMSSF53720. SSF53720. 1 hit.
TIGRFAMsTIGR00069. hisD. 1 hit.
PROSITEPS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHISX_LACLA
AccessionPrimary (citable) accession number: Q02136
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 4, 2005
Last modified: February 19, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways