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Protein

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Gene

hisA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Caution

This protein is inactive in the dairy strain IL1403. The histidine biosynthesis pathway is not functional in the dairy strain IL1403.Curated

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase regulatory subunit (hisZ)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
  6. Imidazoleglycerol-phosphate dehydratase (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. Histidinol-phosphatase (hisK)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei8Proton acceptorBy similarity1
Active sitei131Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciLLAC272623:L0070-MONOMER
UniPathwayiUPA00031; UER00009

Names & Taxonomyi

Protein namesi
Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16)
Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
Gene namesi
Name:hisA
Ordered Locus Names:LL1213
ORF Names:L0070
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001420121 – 2391-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomeraseAdd BLAST239

Proteomic databases

PaxDbiQ02131

Interactioni

Protein-protein interaction databases

STRINGi272623.L0070

Structurei

3D structure databases

ProteinModelPortaliQ02131
SMRiQ02131
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HisA/HisF family.Curated

Phylogenomic databases

eggNOGiENOG4105CJV Bacteria
COG0106 LUCA
HOGENOMiHOG000224614
KOiK01814

Family and domain databases

CDDicd04732 HisA, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01014 HisA, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006062 His_biosynth
IPR006063 HisA
IPR023016 Isoase_HisA
IPR011060 RibuloseP-bd_barrel
PfamiView protein in Pfam
PF00977 His_biosynth, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR00007 TIGR00007, 1 hit

Sequencei

Sequence statusi: Complete.

Q02131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIIPAIDLQ NGEAVRLYKG DYDKKTVYSK NPLEIAQKFE RMGATDLHLV
60 70 80 90 100
DLDGAKIGQT RNLELVRKIK DETRLKIEIG GGIRDFDTVR MYLEQIGVER
110 120 130 140 150
VILGTAAVEK PDFLKELLIK YGPSRIVVGV DIREGFVSTS GWLEKTSLPY
160 170 180 190 200
LSFLKKLERI GVKTTIITDI SKDGTLTGPN FKLYDEISKE NSLNVIISGG
210 220 230
VKDNSDIQRA TRSDFYGIIV GKAYYEGKIN LEKEFRNAN
Length:239
Mass (Da):26,896
Last modified:January 4, 2005 - v3
Checksum:i6690A27AF8FB0411
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73T → S (PubMed:7687248).Curated1
Sequence conflicti191N → S in AAK05311 (PubMed:11337471).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti31N → T in strain: IL1403. 1
Natural varianti54 – 56GAK → DAN in strain: IL1403. 3
Natural varianti65L → I in strain: IL1403. 1
Natural varianti71D → E in strain: IL1403. 1
Natural varianti125 – 127RIV → KII in strain: IL1403. 3
Natural varianti227G → K in strain: IL1403. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92974 Genomic DNA Translation: AAB81908.1
AE005176 Genomic DNA Translation: AAK05311.1
PIRiA47754
E86776
RefSeqiNP_267369.1, NC_002662.1

Genome annotation databases

EnsemblBacteriaiAAK05311; AAK05311; L0070
GeneIDi1114861
KEGGilla:L0070
PATRICifig|272623.7.peg.1310

Similar proteinsi

Entry informationi

Entry nameiHIS4_LACLA
AccessioniPrimary (citable) accession number: Q02131
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 4, 2005
Last modified: June 20, 2018
This is version 122 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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