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Protein

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Gene

hisA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei8 – 81Proton acceptorBy similarity
Active sitei131 – 1311Proton donorBy similarity

GO - Molecular functioni

  1. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. histidine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1301-MONOMER.
UniPathwayiUPA00031; UER00009.

Names & Taxonomyi

Protein namesi
Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16)
Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
Gene namesi
Name:hisA
Ordered Locus Names:LL1213
ORF Names:L0070
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2392391-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerasePRO_0000142012Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272623.L0070.

Structurei

3D structure databases

ProteinModelPortaliQ02131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HisA/HisF family.Curated

Phylogenomic databases

eggNOGiCOG0106.
HOGENOMiHOG000224614.
KOiK01814.
OrthoDBiEOG6H1Q3W.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01014. HisA.
InterProiIPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR006063. HisA.
IPR023016. Isoase_HisA.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00977. His_biosynth. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00007. TIGR00007. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02131-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIIPAIDLQ NGEAVRLYKG DYDKKTVYSK NPLEIAQKFE RMGATDLHLV
60 70 80 90 100
DLDGAKIGQT RNLELVRKIK DETRLKIEIG GGIRDFDTVR MYLEQIGVER
110 120 130 140 150
VILGTAAVEK PDFLKELLIK YGPSRIVVGV DIREGFVSTS GWLEKTSLPY
160 170 180 190 200
LSFLKKLERI GVKTTIITDI SKDGTLTGPN FKLYDEISKE NSLNVIISGG
210 220 230
VKDNSDIQRA TRSDFYGIIV GKAYYEGKIN LEKEFRNAN
Length:239
Mass (Da):26,896
Last modified:January 4, 2005 - v3
Checksum:i6690A27AF8FB0411
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731T → S(PubMed:7687248)Curated
Sequence conflicti191 – 1911N → S in AAK05311. (PubMed:11337471)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti31 – 311N → T in strain: IL1403.
Natural varianti54 – 563GAK → DAN in strain: IL1403.
Natural varianti65 – 651L → I in strain: IL1403.
Natural varianti71 – 711D → E in strain: IL1403.
Natural varianti125 – 1273RIV → KII in strain: IL1403.
Natural varianti227 – 2271G → K in strain: IL1403.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92974 Genomic DNA. Translation: AAB81908.1.
AE005176 Genomic DNA. Translation: AAK05311.1.
PIRiA47754.
E86776.
RefSeqiNP_267369.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05311; AAK05311; L0070.
GeneIDi1114861.
KEGGilla:L0070.
PATRICi22294804. VBILacLac136773_1310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92974 Genomic DNA. Translation: AAB81908.1.
AE005176 Genomic DNA. Translation: AAK05311.1.
PIRiA47754.
E86776.
RefSeqiNP_267369.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ02131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L0070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05311; AAK05311; L0070.
GeneIDi1114861.
KEGGilla:L0070.
PATRICi22294804. VBILacLac136773_1310.

Phylogenomic databases

eggNOGiCOG0106.
HOGENOMiHOG000224614.
KOiK01814.
OrthoDBiEOG6H1Q3W.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00009.
BioCyciLLAC272623:GHSH-1301-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01014. HisA.
InterProiIPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR006063. HisA.
IPR023016. Isoase_HisA.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00977. His_biosynth. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00007. TIGR00007. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Histidine biosynthesis genes in Lactococcus lactis subsp. lactis."
    Delorme C., Ehrlich S.D., Renault P.
    J. Bacteriol. 174:6571-6579(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCDO 2118.
  2. "Gene inactivation in Lactococcus lactis: histidine biosynthesis."
    Delorme C., Godon J.-J., Ehrlich S.D., Renault P.
    J. Bacteriol. 175:4391-4399(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: IL1403.
  3. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiHIS4_LACLA
AccessioniPrimary (citable) accession number: Q02131
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 4, 2005
Last modified: January 7, 2015
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

This protein is inactive in the dairy strain IL1403. The histidine biosynthesis pathway is not functional in the dairy strain IL1403.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.