Q02131 (HIS4_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
EC=5.3.1.16

Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	hisA
Ordered Locus Names:	LL1213
ORF Names:	L0070

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. lactis

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_01014
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP-Rule MF_01014
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HisA/HisF family.
Caution	This protein is inactive in the dairy strain IL1403. The histidine biosynthesis pathway is not functional in the dairy strain IL1403.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP-Rule MF_01014		PRO_0000142012
Sites
	Active site	8	1	Proton acceptor By similarity
	Active site	131	1	Proton donor By similarity
Natural variations
	Natural variant	31	1	N → T in strain: IL1403.
	Natural variant	54 – 56	3	GAK → DAN in strain: IL1403.
	Natural variant	65	1	L → I in strain: IL1403.
	Natural variant	71	1	D → E in strain: IL1403.
	Natural variant	125 – 127	3	RIV → KII in strain: IL1403.
	Natural variant	227	1	G → K in strain: IL1403.
Experimental info
	Sequence conflict	73	1	T → S Ref.2
	Sequence conflict	191	1	N → S in AAK05311. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q02131 [UniParc]. Last modified January 4, 2005. Version 3. Checksum: 6690A27AF8FB0411	FASTA	239	26,896
	10 20 30 40 50 60 MKIIPAIDLQ NGEAVRLYKG DYDKKTVYSK NPLEIAQKFE RMGATDLHLV DLDGAKIGQT 70 80 90 100 110 120 RNLELVRKIK DETRLKIEIG GGIRDFDTVR MYLEQIGVER VILGTAAVEK PDFLKELLIK 130 140 150 160 170 180 YGPSRIVVGV DIREGFVSTS GWLEKTSLPY LSFLKKLERI GVKTTIITDI SKDGTLTGPN 190 200 210 220 230 FKLYDEISKE NSLNVIISGG VKDNSDIQRA TRSDFYGIIV GKAYYEGKIN LEKEFRNAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Histidine biosynthesis genes in Lactococcus lactis subsp. lactis." Delorme C., Ehrlich S.D., Renault P. J. Bacteriol. 174:6571-6579(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCDO 2118.
[2]	"Gene inactivation in Lactococcus lactis: histidine biosynthesis." Delorme C., Godon J.-J., Ehrlich S.D., Renault P. J. Bacteriol. 175:4391-4399(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IL1403.
[3]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U92974 Genomic DNA. Translation: AAB81908.1. AE005176 Genomic DNA. Translation: AAK05311.1.
PIR	A47754. E86776.
RefSeq	NP_267369.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q02131.
ModBase	Search...
Protein-protein interaction databases
STRING	272623.L0070.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK05311; AAK05311; L0070.
GeneID	1114861.
KEGG	lla:L0070.
PATRIC	22294804. VBILacLac136773_1310.
Phylogenomic databases
eggNOG	COG0106.
HOGENOM	HOG000224614.
KO	K01814.
ProtClustDB	PRK00748.
Enzyme and pathway databases
BioCyc	LLAC272623:GHSH-1342-MONOMER.
UniPathway	UPA00031; UER00009.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01014. HisA.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR006063. HisA. IPR023016. Isoase_HisA. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00007. TIGR00007. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_LACLA

Accession

Primary (citable) accession number: Q02131

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program