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Q02131 (HIS4_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

EC=5.3.1.16
Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
Gene names
Name:hisA
Ordered Locus Names:LL1213
ORF Names:L0070
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length239 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_01014

Pathway

Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP-Rule MF_01014

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01014.

Sequence similarities

Belongs to the HisA/HisF family.

Caution

This protein is inactive in the dairy strain IL1403. The histidine biosynthesis pathway is not functional in the dairy strain IL1403.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Histidine biosynthesis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processhistidine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2392391-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP-Rule MF_01014
PRO_0000142012

Sites

Active site81Proton acceptor By similarity
Active site1311Proton donor By similarity

Natural variations

Natural variant311N → T in strain: IL1403.
Natural variant54 – 563GAK → DAN in strain: IL1403.
Natural variant651L → I in strain: IL1403.
Natural variant711D → E in strain: IL1403.
Natural variant125 – 1273RIV → KII in strain: IL1403.
Natural variant2271G → K in strain: IL1403.

Experimental info

Sequence conflict731T → S Ref.2
Sequence conflict1911N → S in AAK05311. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q02131 [UniParc].

Last modified January 4, 2005. Version 3.
Checksum: 6690A27AF8FB0411

FASTA23926,896
        10         20         30         40         50         60 
MKIIPAIDLQ NGEAVRLYKG DYDKKTVYSK NPLEIAQKFE RMGATDLHLV DLDGAKIGQT 

        70         80         90        100        110        120 
RNLELVRKIK DETRLKIEIG GGIRDFDTVR MYLEQIGVER VILGTAAVEK PDFLKELLIK 

       130        140        150        160        170        180 
YGPSRIVVGV DIREGFVSTS GWLEKTSLPY LSFLKKLERI GVKTTIITDI SKDGTLTGPN 

       190        200        210        220        230 
FKLYDEISKE NSLNVIISGG VKDNSDIQRA TRSDFYGIIV GKAYYEGKIN LEKEFRNAN 

« Hide

References

« Hide 'large scale' references
[1]"Histidine biosynthesis genes in Lactococcus lactis subsp. lactis."
Delorme C., Ehrlich S.D., Renault P.
J. Bacteriol. 174:6571-6579(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCDO 2118.
[2]"Gene inactivation in Lactococcus lactis: histidine biosynthesis."
Delorme C., Godon J.-J., Ehrlich S.D., Renault P.
J. Bacteriol. 175:4391-4399(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: IL1403.
[3]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U92974 Genomic DNA. Translation: AAB81908.1.
AE005176 Genomic DNA. Translation: AAK05311.1.
PIRA47754.
E86776.
RefSeqNP_267369.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ02131.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L0070.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK05311; AAK05311; L0070.
GeneID1114861.
KEGGlla:L0070.
PATRIC22294804. VBILacLac136773_1310.

Phylogenomic databases

eggNOGCOG0106.
HOGENOMHOG000224614.
KOK01814.
OrthoDBEOG6H1Q3W.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-1301-MONOMER.
UniPathwayUPA00031; UER00009.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01014. HisA.
InterProIPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR006063. HisA.
IPR023016. Isoase_HisA.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamPF00977. His_biosynth. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 1 hit.
TIGRFAMsTIGR00007. TIGR00007. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHIS4_LACLA
AccessionPrimary (citable) accession number: Q02131
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 4, 2005
Last modified: May 14, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways