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Protein

Histidine biosynthesis bifunctional protein HisIE

Gene

hisI

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate.
1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase regulatory subunit (hisZ)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
  6. Imidazoleglycerol-phosphate dehydratase (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. Histidinol-phosphatase (hisK)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1303-MONOMER.
UniPathwayiUPA00031; UER00007.
UPA00031; UER00008.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine biosynthesis bifunctional protein HisIE
Including the following 2 domains:
Phosphoribosyl-AMP cyclohydrolase (EC:3.5.4.19)
Short name:
PRA-CH
Phosphoribosyl-ATP pyrophosphatase (EC:3.6.1.31)
Short name:
PRA-PH
Gene namesi
Name:hisI
Synonyms:hisIE
Ordered Locus Names:LL1215
ORF Names:L0072
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Histidine biosynthesis bifunctional protein HisIEPRO_0000136418Add
BLAST

Proteomic databases

PaxDbiQ02130.

Interactioni

Protein-protein interaction databases

STRINGi272623.L0072.

Structurei

3D structure databases

ProteinModelPortaliQ02130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 109109Phosphoribosyl-AMP cyclohydrolaseAdd
BLAST
Regioni110 – 212103Phosphoribosyl-ATP pyrophosphohydrolaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the PRA-CH family.Curated
In the C-terminal section; belongs to the PRA-PH family.Curated

Phylogenomic databases

eggNOGiENOG4105K8F. Bacteria.
COG0139. LUCA.
COG0140. LUCA.
HOGENOMiHOG000277503.
KOiK11755.
OMAiERSCFHQ.
OrthoDBiEOG6PGKB6.

Family and domain databases

HAMAPiMF_01019. HisIE.
MF_01020. HisE.
MF_01021. HisI.
InterProiIPR023019. His_synth_HisIE.
IPR008179. HisE.
IPR026660. PRA-CH.
IPR021130. PRib-ATP_PPHydrolase-like.
IPR002496. PRib_AMP_CycHydrolase_dom.
[Graphical view]
PfamiPF01502. PRA-CH. 1 hit.
PF01503. PRA-PH. 1 hit.
[Graphical view]
ProDomiPD002610. PRA_CycHdrlase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03188. histidine_hisI. 1 hit.

Sequencei

Sequence statusi: Complete.

Q02130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPDFHKQEL IPVIVQDYQT NQVLMLAYTN EVAFEKMLET GETWFWSRSR
60 70 80 90 100
QKLWHKGEES GHFQKIKGMR LDCDQDTLLV FVEQIGNACH TGTYSCFYDE
110 120 130 140 150
LIPFDDSDIF SELEKQIIDR KLHPVEKSYT NYLLGEGIDK VLKKVGEEAS
160 170 180 190 200
EVIIASKNSD KGELLGEIDD LLYHLFVLMN QQGISLEEVR QKAKERHQLE
210
GNKKEFHTRT AD
Length:212
Mass (Da):24,717
Last modified:April 27, 2001 - v2
Checksum:i7686EB6B923323E1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 931T → A in AAB81910 (PubMed:1400209).Curated
Sequence conflicti153 – 1531I → T in AAB81910 (PubMed:1400209).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92974 Genomic DNA. Translation: AAB81910.1.
AE005176 Genomic DNA. Translation: AAK05313.1.
PIRiC47754.
G86776.
RefSeqiNP_267371.1. NC_002662.1.
WP_003131119.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05313; AAK05313; L0072.
GeneIDi1114863.
KEGGilla:L0072.
PATRICi22294808. VBILacLac136773_1312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92974 Genomic DNA. Translation: AAB81910.1.
AE005176 Genomic DNA. Translation: AAK05313.1.
PIRiC47754.
G86776.
RefSeqiNP_267371.1. NC_002662.1.
WP_003131119.1. NC_002662.1.

3D structure databases

ProteinModelPortaliQ02130.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L0072.

Proteomic databases

PaxDbiQ02130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05313; AAK05313; L0072.
GeneIDi1114863.
KEGGilla:L0072.
PATRICi22294808. VBILacLac136773_1312.

Phylogenomic databases

eggNOGiENOG4105K8F. Bacteria.
COG0139. LUCA.
COG0140. LUCA.
HOGENOMiHOG000277503.
KOiK11755.
OMAiERSCFHQ.
OrthoDBiEOG6PGKB6.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00007.
UPA00031; UER00008.
BioCyciLLAC272623:GHSH-1303-MONOMER.

Family and domain databases

HAMAPiMF_01019. HisIE.
MF_01020. HisE.
MF_01021. HisI.
InterProiIPR023019. His_synth_HisIE.
IPR008179. HisE.
IPR026660. PRA-CH.
IPR021130. PRib-ATP_PPHydrolase-like.
IPR002496. PRib_AMP_CycHydrolase_dom.
[Graphical view]
PfamiPF01502. PRA-CH. 1 hit.
PF01503. PRA-PH. 1 hit.
[Graphical view]
ProDomiPD002610. PRA_CycHdrlase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03188. histidine_hisI. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Histidine biosynthesis genes in Lactococcus lactis subsp. lactis."
    Delorme C., Ehrlich S.D., Renault P.
    J. Bacteriol. 174:6571-6579(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCDO 2118.
  2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiHIS2_LACLA
AccessioniPrimary (citable) accession number: Q02130
Secondary accession number(s): Q9CG90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 27, 2001
Last modified: July 6, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.