Q02129 (HIS1_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP phosphoribosyltransferase
Short name=ATP-PRT
Short name=ATP-PRTase
EC=2.4.2.17

Gene names

Name:	hisG
Ordered Locus Names:	LL1208
ORF Names:	L0066

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity. HAMAP MF_01018
Catalytic activity	1-(5-phospho-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01018
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9. HAMAP MF_01018
Subunit structure	Heterooctamer composed of four hisG and four hisZ subunits Probable. Ref.4
Subcellular location	Cytoplasm By similarity HAMAP MF_01018.
Domain	Lacks the C-terminal regulatory region which is replaced by hisZ. HAMAP MF_01018
Miscellaneous	The stability and homogeneity of the hisG-hisZ complex is apparently increased by ATP and 5-phosphoribose 1-diphosphate but decreased in the presence of the regulatory inhibitor histidine. HAMAP MF_01018
Sequence similarities	Belongs to the ATP phosphoribosyltransferase family. Short subfamily.
Sequence caution	The sequence AAK05306.1 differs from that shown. Reason: Frameshift at position 174.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 208

208

ATP phosphoribosyltransferase HAMAP MF_01018

PRO_0000151911

Experimental info

Sequence conflict

51

1

P → A in AAB81903. Ref.1

Sequence conflict

86

1

Y → D in AAB81903. Ref.1

Sequence conflict

110

1

H → R in AAB81903. Ref.1

Secondary structure

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208

Helix Strand Turn

Details...

Beta strand

4 – 7

4

Helix

10 – 20

11

Turn

21 – 23

3

Beta strand

36 – 38

3

Beta strand

46 – 49

4

Helix

51 – 59

9

Beta strand

64 – 69

6

Helix

70 – 75

6

Beta strand

81 – 87

7

Beta strand

93 – 98

6

Helix

100 – 104

5

Beta strand

111 – 116

6

Helix

118 – 127

10

Beta strand

132 – 136

5

Helix

143 – 146

4

Beta strand

151 – 160

10

Helix

161 – 164

4

Turn

165 – 167

3

Beta strand

169 – 176

8

Beta strand

179 – 184

6

Helix

185 – 202

18

Sequences

	Sequence		Length	Mass (Da)
	Q02129 [UniParc]. Last modified September 26, 2001. Version 2. Checksum: 8CE4CD0A16D39FEF	FASTA	208	23,677
	10 20 30 40 50 60 MIKIAITKGR IQKQVTKLLE NADYDVEPIL NLGRELQIKT KDDLQIIFGK PNDVITFLEH 70 80 90 100 110 120 GIVDIGFVGK DTLDENDFDD YYELLYLKIG QCIFALASYP DFSNKNFQRH KRIASKYPRV 130 140 150 160 170 180 TKKYFAQKQE DIEIIKLEGS VELGPVVGLA DAIVDIVETG NTLSANGLEV IEKISDISTR 190 200 MIVNKSSFKF KKDKIIEMVE RLEDAQTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Histidine biosynthesis genes in Lactococcus lactis subsp. lactis." Delorme C., Ehrlich S.D., Renault P. J. Bacteriol. 174:6571-6579(1992) [PubMed: 1400209] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCDO 2118.
[2]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
[3]	"An aminoacyl-tRNA synthetase paralog with a catalytic role in histidine biosynthesis." Sissler M., Delorme C., Bond J., Ehrlich S.D., Renault P., Francklyn C. Proc. Natl. Acad. Sci. U.S.A. 96:8985-8990(1999) [PubMed: 10430882] [Abstract] Cited for: CHARACTERIZATION.
[4]	"The quaternary structure of the HisZ-HisG N-1-(5'-phosphoribosyl)-ATP transferase from Lactococcus lactis." Bovee M.L., Champagne K.S., Demeler B., Francklyn C.S. Biochemistry 41:11838-11846(2002) [PubMed: 12269828] [Abstract] Cited for: SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U92974 Genomic DNA. Translation: AAB81903.1.
AE005176 Genomic DNA. Translation: AAK05306.1. Frameshift.

PIR

D45734.
H86775.

RefSeq

NP_267364.1. NC_002662.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z7M	X-ray	2.90	E/F/G/H	1-208	[»]
1Z7N	X-ray	3.25	E/F/G/H	1-208	[»]

ProteinModelPortal

Q02129.

SMR

Q02129. Positions 1-205.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1114855.

GenomeReviews

Gene locus LL1208 in contig AE005176_GR.

KEGG

lla:L0066.

PATRIC

22294790. VBILacLac136773_1303.

Phylogenomic databases

HOGENOM

HBG391868.

ProtClustDB

PRK01686.

Enzyme and pathway databases

BioCyc

LLAC272623:L0066-MONOMER.

Family and domain databases

HAMAP

MF_01018. HisG_Short.
[Tree]

InterPro

IPR013820. ATP_PRibTrfase_cat.
IPR018198. ATP_PRibTrfase_CS.
IPR001348. ATP_PRibTrfase_HisG.
IPR024893. ATP_PRibTrfase_HisG_short.
[Graphical view]

KO

K00765.

PANTHER

PTHR21403. ATP_phspho_trans. 1 hit.

Pfam

PF01634. HisG. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00070. HisG. 1 hit.

PROSITE

PS01316. ATP_P_PHORIBOSYLTR. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HIS1_LACLA

Accession

Primary (citable) accession number: Q02129
Secondary accession number(s): Q9CG94

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	September 26, 2001
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program