Reviewed, UniProtKB/Swiss-Prot Q02126 (E13C_HORVU)

Names and origin

Protein names	Recommended name:     Glucan endo-1,3-beta-glucosidase GIII     EC=3.2.1.39 Alternative name(s):     (1->3)-beta-glucan endohydrolase GIII     (1->3)-beta-glucanase isoenzyme GIII     Beta-1,3-endoglucanase GIII
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May provide a degree of protection against microbial invasion of germinated barley grain through its ability to degrade fungal cell wall polysaccharides.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Developmental stage	Accumulates in developing leaves.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.3
	Chain	26 – 330	305	Glucan endo-1,3-beta-glucosidase GIII		PRO_0000011849
Sites
	Active site	255	1	Nucleophile By similarity
	Active site	312	1	Proton donor By similarity
Experimental info
	Sequence conflict	169	1	D → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q02126-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: B8A262CF345A79E7	FASTA	330	34,887
	10 20 30 40 50 60 MARKGVDVAV ALVLVALAAF PAVHSIGVCN GVLGNNLPAP SDVVTLYRSK RIDAMRIYEP 70 80 90 100 110 120 ESKVLTALSG TGIAVLMDVG PALPSLASSP SAAAAWVKAN VSSFPGVSFR YIAVRNEVMD 130 140 150 160 170 180 SAGQSTILPA MRNVQRALAA AGSPIKVSTS VRFDVFNNTS PPSNGVLADK SGFLRPILNF 190 200 210 220 230 240 LARPARPLLA NVYPYFAYKG NPRDIQLTFA TFVPGSTTVN DNGLTYTNLF DAMVDSIYAA 250 260 270 280 290 300 LEKAGTPGVK VVISESGWPS DQGFGATAQN ARAYNQGLIN HVGNGSPKKA GALESYIFAM 310 320 330 FNENLKDGDE LEKNFGLFKP NMSPAYAITF

References

[1]	"Purification, characterization and gene structure of (1-->3)-beta-glucanase isoenzyme GIII from barley (Hordeum vulgare)." Wang J., Xu P., Fincher G.B. Eur. J. Biochem. 209:103-109(1992) [PubMed: 1396688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Clipper and cv. NK 1558.
[2]	"Evolution and differential expression of the (1-->3)-beta-glucan endohydrolase-encoding gene family in barley, Hordeum vulgare." Xu P., Wang J., Fincher G.B. Gene 120:157-165(1992) [PubMed: 1398132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-330.
[3]	"Purification and properties of three (1-->3)-beta-D-glucanase isoenzymes from young leaves of barley (Hordeum vulgare)." Hrmova M., Fincher G.B. Biochem. J. 289:453-461(1993) [PubMed: 8424790] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-37. Strain: cv. Clipper.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67099 Genomic DNA. Translation: CAA47473.1.
PIR	S29311.
3D structure databases
SMR	Q02126. Positions 26-330.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Organism-specific databases
Gramene	Q02126.
Enzyme and pathway databases
BRENDA	3.2.1.39. 283.
Gene expression databases
Genevestigator	Q02126.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13C_HORVU

Accession

Primary (citable) accession number: Q02126

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)