Reviewed, UniProtKB/Swiss-Prot Q02111 (KPCT_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein kinase C theta type
    EC=2.7.11.13
Alternative name(s):
    nPKC-theta

Gene names

Name:	Prkcq
Synonyms:	Pkcq

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. Ref.1 Ref.2 PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Ref.1 Ref.2
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Enzyme regulation	Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation By similarity.
Subunit structure	Interacts with TXNL2/PICOT By similarity.
Tissue specificity	T-lymphocytes and skeletal muscle. Ref.1 Ref.2
Domain	The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
Post-translational modification	Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from mutant phenotype. Source: MGI membrane protein ectodomain proteolysis Inferred from direct assay. Source: UniProtKB positive regulation of T cell proliferation Inferred from mutant phenotype. Source: MGI positive regulation of interleukin-2 biosynthetic process Inferred from mutant phenotype. Source: MGI protein amino acid phosphorylation Inferred from direct assay. Source: MGI
Cellular component	cytoplasm Inferred from direct assay. Source: MGI immunological synapse Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: UniProtKB protein kinase C activity Inferred from direct assay. Source: MGI zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	Protein kinase C theta type		PRO_0000055709
Regions
	Domain	8 – 123	116	C2
	Domain	380 – 634	255	Protein kinase
	Domain	635 – 706	72	AGC-kinase C-terminal
	Zinc finger	159 – 209	51	Phorbol-ester/DAG-type 1
	Zinc finger	231 – 281	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	386 – 394	9	ATP By similarity
Sites
	Active site	504	1	Proton acceptor By similarity
	Binding site	409	1	ATP By similarity
Amino acid modifications
	Modified residue	219	1	Phosphothreonine; by autocatalysis By similarity
	Modified residue	348	1	Phosphoserine By similarity
	Modified residue	384	1	N6-acetyllysine By similarity
	Modified residue	536	1	Phosphothreonine By similarity
	Modified residue	538	1	Phosphothreonine By similarity
	Modified residue	657	1	Phosphoserine By similarity
	Modified residue	676	1	Phosphoserine By similarity
	Modified residue	685	1	Phosphoserine By similarity
	Modified residue	695	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02111-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 7A16492116CD2880	FASTA	707	81,573
	10 20 30 40 50 60 MSPFLRIGLS NFDCGTCQAC QGEAVNPYCA VLVKEYVESE NGQMYIQKKP TMYPPWDSTF 70 80 90 100 110 120 DAHINKGRVM QIIVKGKNVD LISETTVELY SLAERCRKNN GRTEIWLELK PQGRMLMNAR 130 140 150 160 170 180 YFLEMSDTKD MSEFENEGFF ALHQRRGAIK QAKVHHVKCH EFTATFFPQP TFCSVCHEFV 190 200 210 220 230 240 WGLNKQGYQC RQCNAAIHKK CIDKVIAKCT GSAINSRETM FHKERFKIDM PHRFKVYNYK 250 260 270 280 290 300 SPTFCEHCGT LLWGLARQGL KCDACGMNVH HRCQTKVANL CGINQKLMAE ALAMIESTQQ 310 320 330 340 350 360 ARSLRDSEHI FREGPVEIGL PCSTKNETRP PCVPTPGKRE PQGISWDSPL DGSNKSAGPP 370 380 390 400 410 420 EPEVSMRRTS LQLKLKIDDF ILHKMLGKGS FGKVFLAEFK RTNQFFAIKA LKKDVVLMDD 430 440 450 460 470 480 DVECTMVEKR VLSLAWEHPF LTHMFCTFQT KENLFFVMEY LNGGDLMYHI QSCHKFDLSR 490 500 510 520 530 540 ATFYAAEVIL GLQFLHSKGI VYRDLKLDNI LLDRDGHIKI ADFGMCKENM LGDAKTNTFC 550 560 570 580 590 600 GTPDYIAPEI LLGQKYNHSV DWWSFGVLVY EMLIGQSPFH GQDEEELFHS IRMDNPFYPR 610 620 630 640 650 660 WLEREAKDLL VKLFVREPEK RLGVRGDIRQ HPLFREINWE ELERKEIDPP FRPKVKSPYD 670 680 690 700 CSNFDKEFLS EKPRLSFADR ALINSMDQNM FSNFSFINPG METLICS

References

[1]	"A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle." Osada S., Mizuno K., Saido T.C., Suzuki K., Kuroki T., Ohno S. Mol. Cell. Biol. 12:3930-3938(1992) [PubMed: 1508194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AUTOPHOSPHORYLATION, TISSUE SPECIFICITY.
[2]	"PKC-theta is required for TCR-induced NF-kappaB activation in mature but not immature T lymphocytes." Sun Z., Arendt C.W., Ellmeier W., Schaeffer E.M., Sunshine M.J., Gandhi L., Annes J., Petrzilka D., Kupfer A., Schwartzberg P.L., Littman D.R. Nature 404:402-407(2000) [PubMed: 10746729] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11091 mRNA. Translation: BAA01864.1.
IPI	IPI00124496.
PIR	A44500.
RefSeq	NP_032885.1.
UniGene	Mm.329993
3D structure databases
SMR	Q02111. Positions 3-126, 144-213, 157-252, 227-284, 375-700.
ModBase	Search...
Protein-protein interaction databases
STRING	Q02111.
PTM databases
PhosphoSite	Q02111.
Proteomic databases
PRIDE	Q02111.
Genome annotation databases
Ensembl	ENSMUST00000028118; ENSMUSP00000028118; ENSMUSG00000026778; Mus musculus. [Genome view]
GeneID	18761.
KEGG	mmu:18761.
UCSC	uc008iic.1. mouse.
Organism-specific databases
CTD	18761.
MGI	MGI:97601. Prkcq.
Phylogenomic databases
eggNOG	roNOG07577.
HOGENOM	HBG755340.
HOVERGEN	Q02111.
InParanoid	Q02111.
OMA	AKTNTFC.
PhylomeDB	Q02111.
Enzyme and pathway databases
BRENDA	2.7.11.13. 244.
Gene expression databases
ArrayExpress	Q02111.
Bgee	Q02111.
CleanEx	MM_PRKCQ.
Genevestigator	Q02111.
GermOnline	ENSMUSG00000026778. Mus musculus.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR020454. DAG/PE_bd. IPR011009. Kinase-like_dom. IPR015745. PKC. IPR017892. Pkinase_C. IPR014376. Prot_kin_PKC_delta. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000551. PKC_delta. 1 hit.
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. False negative. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	294949.
SOURCE	Search...

Entry information

Entry name

KPCT_MOUSE

Accession

Primary (citable) accession number: Q02111

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	February 9, 2010
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)