Reviewed, UniProtKB/Swiss-Prot Q02110 (HPPD_PIG)

Names and origin

Protein names

Recommended name:
    4-hydroxyphenylpyruvate dioxygenase
    EC=1.13.11.27
Alternative name(s):
    4-hydroxyphenylpyruvic acid oxidase
      Short name=HPPDase
      Short name=4HPPD
      Short name=HPD

Gene names

Name:

HPD

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	4-hydroxyphenylpyruvate + O(2) = homogentisate + CO(2).
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 3/6.
Subunit structure	Homodimer.
Tissue specificity	Liver and kidney.
Sequence similarities	Belongs to the 4HPPD family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-hydroxyphenylpyruvate dioxygenase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 393	392	4-hydroxyphenylpyruvate dioxygenase		PRO_0000088390
Sites
	Metal binding	183	1	Iron By similarity
	Metal binding	266	1	Iron By similarity
	Metal binding	349	1	Iron By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine
	Modified residue	250	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02110-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: C1A1D42EB2600182	FASTA	393	45,064
	10 20 30 40 50 60 MTSYSDKGEK PERGRFLHFH SVTFWVGNAK QAASYYCSKI GFEPLAYKGL ETGSREVVSH 70 80 90 100 110 120 VVKQDKIVFV FSSALNPWNK EMGDHLVKHG DGVKDIAFEV EDCDYIVQKA RERGAIIVRE 130 140 150 160 170 180 EVCCAADVRG HHTPLDRARQ VWEGTLVEKM TFCLDSRPQP SQTLLHRLLL SKLPKCGLEI 190 200 210 220 230 240 IDHIVGNQPD QEMESASQWY MRNLQFHRFW SVDDTQIHTE YSALRSVVMA NYEESIKMPI 250 260 270 280 290 300 NEPAPGKKKS QIQEYVDYNG GAGVQHIALK TEDIITAIRS LRERGVEFLA VPFTYYKQLQ 310 320 330 340 350 360 EKLKSAKIRV KESIDVLEEL KILVDYDEKG YLLQIFTKPM QDRPTVFLEV IQRNNHQGFG 370 380 390 AGNFNSLFKA FEEEQELRGN LTDTDPNGVP FRL

References

[1]

"Primary structure deduced from complementary DNA sequence and expression in cultured cells of mammalian 4-hydroxyphenylpyruvic acid dioxygenase. Evidence that the enzyme is a homodimer of identical subunits homologous to rat liver-specific alloantigen F." Endo F., Awata H., Tanoue A., Ishiguro M., Eda Y., Titani K., Matsuda I. J. Biol. Chem. 267:24235-24240(1992) [PubMed: 1339442] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ACETYLATION AT THR-2, PARTIAL PROTEIN SEQUENCE. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13390 mRNA. Translation: BAA02660.1.
PIR	S32821.
RefSeq	NP_999389.1.
UniGene	Ssc.16162
3D structure databases
HSSP	HSSP built from PDB template 1JC4 based on UniProtKB Q8VQN0.
SMR	Q02110. Positions 8-366.
ModBase	Search...
Genome annotation databases
GeneID	397443.
KEGG	ssc:397443.
Phylogenomic databases
HOVERGEN	Q02110.
Family and domain databases
InterPro	IPR005956. 4OHPhenylPyrv_dOase. IPR004360. Glyas_bleo-R_dOase. [Graphical view]
PANTHER	PTHR11959. HPP_dOase. 1 hit.
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
PIRSF	PIRSF009283. HPP_dOase. 1 hit.
TIGRFAMs	TIGR01263. 4HPPD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPD_PIG

Accession

Primary (citable) accession number: Q02110

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 58 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)